Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,419,720 T→C 23.9% D183G (GAT→GGT)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,419,7200TC23.9% 65.6 / 15.9 41D183G (GAT→GGT) hisJhistidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (13/17);  new base C (4/6);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGC  >  CP009273/2419579‑2419858
                                                                                                                                             |                                                                                                                                          
tCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGaa                                                                                                                                     <  1:335163/149‑1 (MQ=255)
   cgcAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTg                                                                                                                                        <  2:198992/143‑1 (MQ=255)
     cAGTTCGTTATCTTCTTTACACAGGCCCATGCCGGTCCCTACGCCAAACAGTCTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCgg                                                                                                                                <  1:292028/149‑1 (MQ=255)
        ttCCTTATCTCCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcg                                                                                                                                   <  2:14348/143‑1 (MQ=255)
                 ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCaa                                                                                                                          <  2:224154/143‑1 (MQ=255)
                     ttACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCGGCGACCTCATCATGGAACGCGGCATCAATACg                                                                                                                      >  2:258747/1‑143 (MQ=255)
                         gCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtc                                                                                                            >  1:328289/1‑149 (MQ=255)
                          cAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAGCCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCgg                                                                                                                 <  2:237339/143‑1 (MQ=255)
                          cAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtca                                                                                                           >  1:95602/1‑149 (MQ=255)
                             gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAg                                                                                                              <  2:303209/143‑1 (MQ=255)
                             gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggt                                                                                                        >  1:115516/1‑149 (MQ=255)
                                 aTGCCGGGCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtcag                                                                                                          <  2:160435/143‑1 (MQ=255)
                                          ccTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAACCCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgtt                                                                                           <  1:233605/149‑1 (MQ=255)
                                                        tttttCATCTTTAACAGACGGTCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATTCGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGcc                                                                                   <  2:75206/143‑1 (MQ=255)
                                                        tttttCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATAAGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGcc                                                                                   >  2:115116/1‑143 (MQ=255)
                                                         ttttCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGTGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGccc                                                                                  <  2:238954/143‑1 (MQ=255)
                                                            tCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTGCGGCAGTCAGGTCAGAATAAATGGTGTCCTGCCCCTGATACga                                                                         >  1:326461/1‑149 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCCGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                    >  1:16814/1‑149 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                    >  1:273931/1‑149 (MQ=255)
                                                                     aCAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGAc                                                                      <  2:172109/143‑1 (MQ=255)
                                                                                aCCGAATTTGTAATCTGTACCGCCAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTg                                                     <  1:111082/149‑1 (MQ=255)
                                                                                 ccGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTgg                                                    <  1:85189/149‑1 (MQ=255)
                                                                                  cGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGt                                                   >  1:333100/1‑149 (MQ=255)
                                                                                  cGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGGCCTGCCCCTGATACGAGACGATTTCAATGCCt                                                         >  2:48878/1‑143 (MQ=255)
                                                                                       ttGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCCGGTCAGAATCAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTgg                                                    <  2:135303/143‑1 (MQ=255)
                                                                                           aagcTTTGCCGAGAGGTTGTCTGAGGAAACCTTCGCTGGCAGCGACCTCCTCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAGTGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGcc                                                <  2:155713/140‑1 (MQ=255)
                                                                                               tttACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATATATGTTGTCCTGCCCCTGATACGGGACGATTTCAATGCCTTTTGGTGCCCCATGTTCAt                                      >  1:180485/1‑149 (MQ=255)
                                                                                                  aCCGCCACGTTGTTTGAGGAAACCTTCGCTGGCCGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATCAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTAc                                   <  1:121779/149‑1 (MQ=255)
                                                                                                  aCCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTACTCCGAGGCCATTTCAATGCCTTTTGGTGCCTAATGTTCATTAc                                   >  1:160366/1‑149 (MQ=255)
                                                                                                         ggTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTAcc                                  <  2:259838/143‑1 (MQ=255)
                                                                                                          gttggttgAGGAAACCTGCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGTTACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCg                                 <  2:85411/143‑1 (MQ=255)
                                                                                                            tgttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGaa                               <  2:293980/143‑1 (MQ=255)
                                                                                                             gttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCGCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTcc                        <  1:197894/149‑1 (MQ=255)
                                                                                                             gttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGACAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAAc                              >  2:242190/1‑143 (MQ=255)
                                                                                                                tGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGACCGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGGCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTgg                     <  1:305943/149‑1 (MQ=255)
                                                                                                                  aGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCATTGTTCAGTACCGAACGtctt                         >  2:45969/1‑142 (MQ=255)
                                                                                                                          ttCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAGGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCt           >  1:287222/1‑149 (MQ=255)
                                                                                                                              cTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGcc             >  2:23139/1‑143 (MQ=255)
                                                                                                                                   agtgACCTCACACTGGAACCCGGCATCTATACGTCCGGCCGTCAGGTCCGAATAAATGTTGTCCTGCCCCTCATACGAGACGATTTCACTGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGc  <  1:77225/146‑1 (MQ=255)
                                                                                                                                   aGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCGCAAGGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCACTACGc  >  1:303248/1‑149 (MQ=255)
                                                                                                                                      gcccTCAACCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTGGTCCTGCCCCTGCTACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATa     <  2:158820/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
TCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGC  >  CP009273/2419579‑2419858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑