| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,444,286 | C→T | 26.7% | A296V (GCC→GTC) | yjfF → | inner membrane putative ABC superfamily sugar transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,444,286 | 0 | C | T | 26.7% | 13.5 / 5.3 | 15 | A296V (GCC→GTC) | yjfF | inner membrane putative ABC superfamily sugar transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (7/4); new base T (2/2); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01 | |||||||||||
AGTGGTAATTGGCGGTACGCTTTTGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAG > CP009273/4444149‑4444423 | ccgatctaTTGGCGGTACGCTTTTGAGCGGTGGCGTTGGGACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGATGAGTTCCTGGTGGACGAAAATCGTCATCg < 2:234791/136‑1 (MQ=255)ccgatctaTTGGCGGTACGCTTTTGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGTCATCg < 2:302056/136‑1 (MQ=255) gTAATTGGCGGTACGCTTTTGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTTTTGTTGCTATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGtgt > 2:162504/1‑141 (MQ=255) gTAATTGGCGGTACGCTTTTGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAGATCGCCAGCGGTATTttgtt > 1:14935/1‑149 (MQ=255) ttGGCGGTACGCTTTTGAGCGGTGGCGTTGGGACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGTCATCggagatcggaagagc > 1:262827/1‑136 (MQ=255) ttGGCGGTACGCTTTTGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGTCATCggagatcggaagggc > 1:335101/1‑136 (MQ=255) ttttGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAGGAAAATCGCAATCGGTATTTTGTTGATTAttttt > 2:331706/1‑143 (MQ=255) tttGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCa < 1:343600/149‑1 (MQ=255) gtggcgATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGCTTAACGGTGCTGTGGGAGAAGCGTCAGAGTTc > 1:220555/1‑149 (MQ=255) ggACTGATTCAGACTTACATAACCTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATGACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGt < 2:133951/143‑1 (MQ=255) aaaCTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCCTTACAGCGTGGATTAACGGTGCTGTGCGAGAATCGCCTGAGTTCGCTACTGACAAGAGTCAAAATTgctgcccgat > 1:160833/1‑141 (MQ=255) ttGACGGCACGCTGAGTTCCTGGTGGACGAAAACCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATaaaaa < 1:19028/149‑1 (MQ=255) tGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTTTAGCATTTCAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAGGAGTAAACATTGCGCACCAATGAAAACGCctc > 2:163751/1‑143 (MQ=255) ggtggACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAAAAAAAACGCCTCCCCGCGTgg > 2:122616/1‑143 (MQ=255) gACGCAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGGGGATAATTGGTGCCTTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAg < 1:177159/149‑1 (MQ=255) | AGTGGTAATTGGCGGTACGCTTTTGAGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAG > CP009273/4444149‑4444423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |