| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,313,853 | T→A | 57.5% | I65F (ATC→TTC) | trpC ← | fused indole‑3‑glycerolphosphate synthetase/N‑(5‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,313,853 | 0 | T | A | 57.5% | 2.1 / 10.2 | 14 | I65F (ATC→TTC) | trpC | fused indole‑3‑glycerolphosphate synthetase/N‑(5‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base A (4/4); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
AAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGATCACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCGGCTGAACCTCATTCTGAAAACTGGCCAGCGGTTGCTGCTGTTTGCGGGCTT > CP009273/1313709‑1313993 | aaaTCGGCGGCGGGGCGATCCGGCTGACGATGGGGAGGTTACTTCTACGACCGTTAAAATATTTCTCATCAGTCAGCACCGGAACTGCCGAAGCGGAAGGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGAACACg < 1:84665/149‑1 (MQ=255) tCGGCTGCGGGGTGATTTGGCTGACGATGGGGACGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGATCACGCCt < 1:1156/149‑1 (MQ=255) aaGCGCCCCTGGAAATATTGCTCACCAGTCAGCACCGAAATGGCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGAGCGAAATCATCACGGAACACGCCTTTCGACGGCGACGCTTTCTTGCACTCCAGAATaaa < 2:130099/143‑1 (MQ=255) aaaaTATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGAACACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCt > 1:157690/1‑149 (MQ=255) tatTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGAACACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCAcc < 2:172677/143‑1 (MQ=255) gCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGATCACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCgg < 1:341554/149‑1 (MQ=255) cGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGATCACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTCGCGCGTCATAAAAATGTCGCGTGCTCGGCTGAAcc > 1:9057/1‑149 (MQ=255) cGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCACCCCGAATCAGGACCATTGATGGGGACGTATTCTAGCATCCGAGAATACATGAACTTCGTTAACCCTGTAGCGCATCATAAAAATCTcgcggccccggc > 2:78902/1‑135 (MQ=255) tGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCAAGGAACACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCGGCTGAAc > 2:76725/1‑143 (MQ=255) cAATGCGTGCTGGATCGAAATCATCACGGATCACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGGGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCGGCTGAACCTCATTCTGAAAACTg < 2:952/143‑1 (MQ=255) ggATCGAAATCATCACGGAACACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGAGTGCTCGGCTGAACCTCATTCTGAAAACTGGCCAGCGGTtg > 2:8918/1‑143 (MQ=255) tcatcaCGGATCACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAATAATGTCGCGTGCTCGGCTGAACCTCATTCTGAAAACTGGCCAGCGGTTGCTGCTGTTTGCGGGc < 1:223684/149‑1 (MQ=255) atcaCGGAACACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCGGCTGAACCTCATTCTGAAAACTGGCCAGCGGTTGCTGCTGTTTGCGGGCtt > 1:50732/1‑149 (MQ=255) tcaCGGAACACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCGGCTGAACCTCATTCTGAAAACTGGCCAGCGGTTGCTGCTGTTTGCg < 2:330103/143‑1 (MQ=255) | AAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAAATGGCGGCAATGCGTGCTGGATCGAAATCATCACGGATCACGCCTTTTGACGGCGACGCTTTCTTGCACTCCAGAATAAACGCCGTGCGCGCACCCTGTAGCGCATCATAAAAATGTCGCGTGCTCGGCTGAACCTCATTCTGAAAACTGGCCAGCGGTTGCTGCTGTTTGCGGGCTT > CP009273/1313709‑1313993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |