Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,676,832 G→T 100% intergenic (‑25/+158) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,676,8320GT94.9% 182.4 / 0.4 64intergenic (‑25/+158)dctA/yhjKC4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (38/22);  total (41/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Not supported by required number of reads on both strands.

CCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATC  >  CP009273/3676689‑3676977
                                                                                                                                               |                                                                                                                                                 
ccGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGTATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATGTGGTGTCCTTTAGTAAAACCACATGTCtt                                                                                                                                              <  1:304675/149‑1 (MQ=255)
 cGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTg                                                                                                                                             >  2:460163/1‑149 (MQ=255)
          gCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                    <  1:269246/149‑1 (MQ=255)
              tttCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAACACCACATGTCTTGTAAGGTTATgggg                                                                                                                                >  2:115310‑M1/1‑145 (MQ=255)
                  aTTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTCAGGTCATggagtacc                                                                                                                            >  1:435048‑M1/1‑141 (MQ=255)
                        tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggg                                                                                                                      <  2:299760‑M1/149‑15 (MQ=255)
                           ccTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATAGGGTACCTGCGGCCtt                                                                                                                   >  1:136424/1‑149 (MQ=255)
                              aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGATGATTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacct                                                                                                                <  2:376199‑M1/149‑21 (MQ=255)
                               tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGGTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctg                                                                                                               >  1:414875‑M1/1‑128 (MQ=255)
                                        cgcgagtgcgaaggagaataccAATGGCTCGCGCTGTCAGGACCTGAGAGTAAAGGCTTTTAAACAGGGAGGTGTTCATGGGGTGTCCTGTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaa                                                                                                      <  2:302953‑M1/139‑31 (MQ=255)
                                         gAAATGGCCAAGGAGAATACCAAGGGCTATCGCTGTCAGGACCTGTAACTAGAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGCCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaa                                                                                                     <  1:13469‑M1/149‑32 (MQ=255)
                                          aaaTGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaa                                                                                                    >  1:315167‑M1/1‑117 (MQ=255)
                                          aaaTGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATACCACCCCGa                                                                                                    >  2:344091/1‑149 (MQ=255)
                                          aaaTGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGGTTTCATAGGGTGTCCTTTAGTGAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtgcctgcggccgtgaaa                                                                                                    >  1:391040‑M1/1‑117 (MQ=255)
                                             tGGCCAAGGAGAATACCAATGGCTATCGCTGCCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataa                                                                                                 <  2:256321‑M1/149‑36 (MQ=255)
                                                ccAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaacataacac                                                                                              >  1:435245‑M1/1‑111 (MQ=255)
                                                ccAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacac                                                                                              <  1:405121‑M1/149‑39 (MQ=255)
                                                ccAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacac                                                                                              >  2:45892‑M1/1‑111 (MQ=255)
                                                 caaggtgtctaccaatggcTATCGCGGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCATGTAAGGTTATggggtagctgcgggggacctgcgggcgtaaaataagacc                                                                                             >  2:311040‑M1/10‑110 (MQ=255)
                                                 cAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacc                                                                                             >  1:86266‑M1/1‑110 (MQ=255)
                                                 cAAGGAAAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTCTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtatctgcagggtacctgcggccttaaaatcacacg                                                                                             >  2:406961‑M1/1‑110 (MQ=255)
                                                   aGGAGAATACCCATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacaccca                                                                                           <  2:426919‑M1/149‑42 (MQ=255)
                                                   aGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacaccca                                                                                           >  1:250701‑M1/1‑108 (MQ=255)
                                                     gagaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAGACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccaga                                                                                         >  1:334678‑M1/1‑106 (MQ=255)
                                                     gagaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccaga                                                                                         >  2:434320‑M1/1‑106 (MQ=255)
                                                        aaTACCAATGGCTATCGCTGTCAGGACTTGAAAGTAAAGGCTTTTAAAGAGAGAGGTTTTCATAGGGGTTCATTTAGTAAAACCACAGGTGTTGTAGGGCGATGGGGTATCTGCGGGCTTAAAATAACACCCGGGCAACATCATAGaag                                                                                      >  1:159252/1‑148 (MQ=255)
                                                        aaTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaa                                                                                      >  2:260712‑M1/1‑103 (MQ=255)
                                                             cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGCGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacaccccgacaacatca                                                                                 <  1:137791‑M1/149‑52 (MQ=255)
                                                             cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacacca                                                                                 >  2:246584‑M1/1‑98 (MQ=255)
                                                              aagggctaTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcac                                                                                <  1:377253‑M1/146‑53 (MQ=255)
                                                               aTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcaca                                                                               <  1:449171‑M1/149‑54 (MQ=255)
                                                                    tATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaat                                                                          <  1:444745‑M1/149‑59 (MQ=255)
                                                                    tATCGCTGCCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAACACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgtggccttaaaataacacccagacaacatcacagaaat                                                                          <  1:142566‑M1/149‑59 (MQ=255)
                                                                         cTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacc                                                                     >  1:264723‑M1/1‑86 (MQ=255)
                                                                            tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTCTggcgtgccttcggggtacctgcggccttaaaataaaacccagacaaaatcacagaaatgtacctgg                                                                  >  1:109550‑M1/1‑83 (MQ=255)
                                                                            tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctgg                                                                  >  1:452436‑M1/1‑83 (MQ=255)
                                                                            tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctgg                                                                  >  1:452415‑M1/1‑83 (MQ=255)
                                                                              aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggat                                                                >  1:165751‑M1/1‑81 (MQ=255)
                                                                              aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggat                                                                <  1:210813‑M1/149‑69 (MQ=255)
                                                                              aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggat                                                                <  1:135179‑M1/149‑69 (MQ=255)
                                                                                     gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataatt                                                         <  1:468573‑M1/149‑76 (MQ=255)
                                                                                     gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataatt                                                         >  2:18613‑M1/1‑74 (MQ=255)
                                                                                      aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacaccccgacaacatcacagaaatgtacctggatcgtaattg                                                        >  1:403926‑M1/1‑73 (MQ=255)
                                                                                          tAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaac                                                    >  2:256810‑M1/1‑69 (MQ=255)
                                                                                               gCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaa                                               >  2:236443‑M1/1‑64 (MQ=255)
                                                                                                 ttttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaagg                                             >  1:417916‑M1/1‑62 (MQ=255)
                                                                                                  tttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaagga                                            >  1:263389‑M1/1‑61 (MQ=255)
                                                                                                   ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccaggcaacatcacagaaatgtacctggatcataattgaaacaaaaaggtt                                           <  1:390650‑M1/149‑90 (MQ=255)
                                                                                                       aCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctgggtaataattgaaacaaaaaggataaaa                                       >  1:2325‑M1/1‑56 (MQ=255)
                                                                                                          gagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagt                                    <  2:26023‑M1/149‑97 (MQ=255)
                                                                                                           agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtt                                   <  1:45892‑M1/149‑98 (MQ=255)
                                                                                                            gagGTTTGCATAAGGTTGCCTTTAGTAAAACAAAATGTATTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcatagaaatgtacctggattataattaaaacaaaaaggttaaaaagttt                                  <  2:406648‑M1/149‑99 (MQ=255)
                                                                                                             agGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcgggttatctgcggccttaaaataacagccagacaacatcacaggaaggtacctggagcataattgaaccaaagaggttaaaacgtttg                                 >  2:375500‑M1/1‑50 (MQ=255)
                                                                                                                      tAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctc                        >  2:109214‑M1/1‑41 (MQ=255)
                                                                                                                         ggTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaatcacacccagacaacatcacagcaatgtacctggatcatcattgaaacaaaaagggtaaaaagttttagttggctcggt                     >  2:16637‑M1/1‑38 (MQ=255)
                                                                                                                             tCCTTTAGTAAAACCACATGTCTTGCAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacgtcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaa                 <  1:287413‑M1/149‑116 (MQ=255)
                                                                                                                               cTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtagctgaggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttacgctggctcgcacaaacc               >  1:366116‑M1/1‑32 (MQ=255)
                                                                                                                                tttAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtagctgcggccttaaaataacacccagacaacatcacagaaatgtacctgggtcataattgacacaccaagcataaaaagattgagctggctcgcacaaacca              >  1:242889‑M1/1‑31 (MQ=255)
                                                                                                                                tttAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaacggttcaaaagtttgagctggctcgcaccaacca              >  2:394789‑M1/1‑31 (MQ=255)
                                                                                                                                tttAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaacca              >  2:296550‑M1/1‑31 (MQ=255)
                                                                                                                                   aGTAAAGCCACATGTCTTGTAAGGGTATggggtacgtgaggggtacctgcggccataaaataacacccagagaacatcacagaaatgtacttggatcaaaaaaaaaaaaaaaaggttaaaaagtttgagctggctcgcacaaaccagca           <  2:245888‑M1/149‑122 (MQ=255)
                                                                                                                                    gTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaaccagcac          >  1:116230‑M1/1‑27 (MQ=255)
                                                                                                                                     tAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaaccagcact         >  1:3717‑M1/1‑26 (MQ=255)
                                                                                                                                            acaTGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaaccagcactttttaaa  >  1:428388‑M1/1‑19 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATC  >  CP009273/3676689‑3676977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: