| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,305,945 | C→G | 69.3% | R95P (CGT→CCT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,305,945 | 0 | C | G | 69.3% | 54.8 / 33.6 | 39 | R95P (CGT→CCT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (3/9); new base G (9/18); total (12/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACAT > CP009273/2305805‑2306083 | tGAAGTTGTCAGAACCGTAGCTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg < 1:150057/143‑1 (MQ=255) ttGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAgtt > 2:437054/1‑149 (MQ=255) aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTTCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttcgt < 2:14673/149‑1 (MQ=255) aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTgttttcgt < 2:393314/149‑1 (MQ=255) ggTGTCACCACCGAATGCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTgttttcgt < 1:445713/143‑1 (MQ=255) ctgtCACCACCGAATTCTGGCAGCACCTCGGTGCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCACGAGTTgttttcgtt < 1:207887/142‑1 (MQ=255) tcgccaccGAAGTCGGGCAGTACGTCGGTCCAGGAAGTTCCGTCTTAAACATCGCCGGAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCACACCTGCTAATGCCACAGGCGTCCAGGAGTTgttttcgttttc < 1:376255/140‑1 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAgcg > 1:544105/1‑143 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTg > 1:381592/1‑143 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATc < 2:215099/149‑1 (MQ=255) tGGCAGTACGTCGGTCCGGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCACACCTGCGTATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCt < 1:355869/143‑1 (MQ=255) tGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCt < 2:486253/149‑1 (MQ=255) ggCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTg < 2:192302/149‑1 (MQ=255) gaaGTACGTCGGGCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTgg < 1:13992/141‑1 (MQ=255) gCAGTACGGCGGTCCAGGAAGTTACGTCATAGACAACGCCGTAGTTACGACCGTAGTCGTAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTgg < 1:427427/143‑1 (MQ=255) aGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGata > 2:605982/1‑149 (MQ=255) aGTACGCCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCCGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGAt < 1:523812/143‑1 (MQ=255) cggcGGCCCAGGAAGTGACGTCGTAACCAACGCGGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGa < 1:23665/140‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGGCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCtta < 1:70946/143‑3 (MQ=255) gTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCGGat > 1:434860/1‑143 (MQ=255) gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTg > 2:209537/1‑149 (MQ=255) ggAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCGCTGGCCGt > 2:34288/1‑149 (MQ=255) ggAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACATGCGAATGCCACCGGGGACCAGGAGCTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATCTTCCCCCTGGCCgg > 2:246102/1‑148 (MQ=255) aaGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTgg < 1:485031/143‑1 (MQ=255) cGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTc > 2:638226/1‑149 (MQ=255) aTAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCt < 2:390147/149‑1 (MQ=255) acaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTGCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAg < 1:183825/143‑1 (MQ=255) cGTAGTTACGAGCGGAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCa < 1:396448/143‑1 (MQ=255) aCCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc < 2:229168/149‑1 (MQ=255) gTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCAGCTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc < 1:376215/143‑1 (MQ=255) tCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAcc > 1:245609/1‑143 (MQ=255) ccACCTCCTGGACTTTCAGACGTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTTTTGCTCTGGTTCTTATATTCCCACCGGCCGTAACCGGTCAGCTGGCCAGTATCCTGAGtttttcggatttagcga < 1:236006/143‑15 (MQ=255) ccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCa < 1:208654/143‑1 (MQ=255) acaTCCTGGAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCAt < 2:602011/149‑1 (MQ=255) ggAATTTCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTAGGGAGCAACACTGATGACCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTc > 2:628482/1‑149 (MQ=255) ttCAGACCTGCGAATGCCACAGGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTc < 2:286640/149‑1 (MQ=255) ttCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCTCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGt < 1:228064/143‑1 (MQ=255) tGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGTTCAGCTGGTCAGTAACCTGAGTTCCGCAGCGCGAGCCAAGACGCATGTAGGTCTGGTCGc < 1:405544/143‑1 (MQ=255) cacaCGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACAt > 1:419917/1‑143 (MQ=255) | TGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACAT > CP009273/2305805‑2306083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |