New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2897623 =93 (1.700)5 (0.100) 5/278 6.3 5.3% intergenic (+890/+483) ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
?CP009273 2897641 = 90 (1.730)intergenic (+908/+465) ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTATGTTTAGAGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAACCTGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2897777‑2897623
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcggggaACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTTTTGCGATTGCCCGGTTTTTGCCGGTTTATCCCCGCTGGCGCGGGGAACACTCTAAACATAACCTATT  >  CP009273/2897641‑2897783
                                                                                                                                                                                                                                                                                                          
TTATGTTTAGAGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAGAAAGGCGTAATGTGTTCCCCGCGCCGGCGGGGATAAACCTGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAG                                                                                                                                                       >  1:608858/1‑149
TTATGTTTAGAGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAACCTGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAG                                                                                                                                                       >  1:382720/1‑149
  ATGTTTAGAGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAACCTGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCG                                                                                                                                                     <  1:897733/149‑1
    GTTTAGAGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAACATGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGG                                                                                                                                                   >  1:524090/1‑149
    GTTTAGAGTGTCCCCCGCGCACGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAAACCGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGG                                                                                                                                                   <  1:31098/149‑1
         ggGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAACCTGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGG                                                                                                                                                   <  2:67737/142‑1
                                                                                                            CGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTTTTGCGATTGCCCGGTTTTTGCCGGgt                                           >  1:838222/1‑147
                                                                                                              GCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCAAGCGGGGAACACGAAATGCTGGTGAGCGTTAATGACGCAAACACAGGTTTATCCCCGCTGGGGCGGGGAACACATTACGACTTTTTGTGTTTGCCGGGTTTTTGCCa                                              >  2:772380/1‑143
                                                                                                                       CTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTTTTGCGATTGCCCGGTTTTTGCCGGTTTATCCCCGCTG                                >  1:348141/1‑149
                                                                                                                        TCACCAGCATTTCGTGTTCCCCGCGCCAGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCAGCAAACACAGGATTATCCCCGCTGGCGCGGGGAACACATTACGCCTTTTTGCGATTGCCCGGTTTTTGCCGGTTTATCCCC                                    >  2:697925/1‑144
                                                                                                                                      TGTTCCCCGCGCCAGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTTTTGCGATTGCCCGGTTTTTGCCGGTTTATCCCCGCTGGCGCGGGGAACACTC                 >  1:354252/1‑149
                                                                                                                                                          AACACGAAATGCTGGTGAGCGTTAATGCCGCCAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTCCGCCTTTTTGCGACTGCCCGGTTTCTGCCGGCTTATCCCCCCTGGCCCGGGGCAACCGCTAAACATAACCCATT  >  2:153230/1‑144
                                                                                                                                                                                                                                                                                                          
TTATGTTTAGAGTGTTCCCCGCGCCAGCGGGGATAAACCGGCAAAAACCGGGCAATCGCAAAAAGGCGTAATGTGTTCCCCGCGCCAGCGGGGATAAACCTGTGTTTGCGGCATTAACGCTCACCAGCATTTCGTGTTCCCCGCGCCAGCGGGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2897777‑2897623
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcggggaACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTTTTGCGATTGCCCGGTTTTTGCCGGTTTATCCCCGCTGGCGCGGGGAACACTCTAAACATAACCTATT  >  CP009273/2897641‑2897783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.