Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	CP009273	4,145,501	Δ320 bp	coding (573‑892/1005 nt)	argC →	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4145501	4145820	320	61 [0]	[0] 61	argC	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4145500	0 (0.000)	57 (1.090)	45/278	0.2	100%	coding (572/1005 nt)	argC	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding
	?	CP009273	4145821 =	0 (0.000)					coding (893/1005 nt)	argC	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

TCGGGTGTGACCCAGGCGCAAGTCGCGCAAGTGTTACAGCAGGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTG  >  CP009273/4145673‑4145967                                                                                                                                                     |                                                                                                                                                   aattaaaagaagcgaatttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcga                                                                                                                                                    <  1:870824‑M2/1‑1 (MQ=255)   ttaaaagaagcgaatttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccgggtgtctacgccaccagcggcgtgagcggtgcagggcgAGc                                                                                                                                                  >  1:630208‑M2/147‑149 (MQ=255)    taaaagaagcgaatttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCa                                                                                                                                                 >  1:129003‑M2/146‑149 (MQ=255)        agaagcggattggagggcggggccggggtggtatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaagcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGc                                                                                                                                                  <  2:797298‑M2/3‑1 (MQ=255)             cgaatttgattgcggtgccgggctgttaccagacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGattat                                                                                                                                        <  1:863528‑M2/13‑1 (MQ=255)                 tttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATtgtg                                                                                                                                    <  1:539012‑M2/17‑1 (MQ=255)                 tttgattccggtgccgggctgttatccgacggtggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATtgtg                                                                                                                                    <  1:474031‑M2/17‑1 (MQ=255)                    gattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGCTTATtg                                                                                                                                      <  2:542065‑M2/15‑1 (MQ=255)                      ttgcggtgccaggctgtaaaccgaaagcggaacagctggagctaaaaccattgattgatgccgatcttcttgacctcactcagtggccggtgatcaacgccaccagcggcgtgagctgtgcagggcgAGCATCTGATTATTGTGGCGAc                                                                                                                               <  1:886973‑M2/22‑1 (MQ=255)                             gccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCg                                                                                                                             <  2:314064‑M2/24‑1 (MQ=255)                              tcgggctgttatcggacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACa                                                                                                                       <  1:53500‑M2/30‑1 (MQ=255)                                 ggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGa                                                                                                                         <  2:315023‑M2/28‑1 (MQ=255)                                 ggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACt                                                                                                                    <  1:128456‑M2/33‑1 (MQ=255)                                  gctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGAc                                                                                                                        <  2:263625‑M2/29‑1 (MQ=255)                                   ctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgccctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAAATTa                                                                                                                  >  1:243885‑M2/115‑149 (MQ=255)                                    tgttatccgacggcggcacagctggcgcggaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatccacgcaacgagcggcatgcatgatccagggcgAGCATCTGATTATTGTGTCGACCGAAGACAACTTAc                                                                                                                 <  1:681010‑M2/36‑1 (MQ=255)                                         tccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCGTCTGAGTATTGTGGCGACCGAAGGCAACTTAc                                                                                                                 >  2:403826‑M2/109‑144 (MQ=255)                                                 cgggcccgcgggcgctgaaaccgttgattgatgccgatctccttgacctcaatcagtggccggtgatcaacgccaccaccggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCgcggc                                                                                                    <  1:67874‑M2/49‑1 (MQ=255)                                                   gcacagctggcgctgaaaccgttgaatgataccgatcttcttgacctcaatcagtggccggtgatccacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGcggcgg                                                                                                  >  1:481619‑M2/99‑149 (MQ=255)                                                    cacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggcggc                                                                                                 >  1:927018‑M2/98‑149 (MQ=255)                                                      cagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatccgtggccggtgatcaacgctaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCgcggc                                                                                                    >  2:820076‑M2/96‑144 (MQ=255)                                                       agctggcgctgaaaccgtggattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCgcggcg                                                                                                   <  2:793425‑M2/50‑1 (MQ=255)                                                         ctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggcggc                                                                                                 >  2:212597‑M2/93‑144 (MQ=255)                                                          tggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGc                                                                                           >  1:232664‑M2/92‑149 (MQ=255)                                                               ctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGCAAGGGGCGGCGGCCCAAGc                                                                                           >  2:207386‑M2/87‑144 (MQ=255)                                                                tgaaaccgttgattgatgccgatcttcttgacctcaatccgtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCg                                                                                          <  2:840553‑M2/59‑1 (MQ=255)                                                                tgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGAAAGTTTTTTTGTTGGCGCGGCGGCACAAGCGGTACa                                                                                     <  1:225307‑M2/64‑1 (MQ=255)                                                                tgaaaccgttgatcgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACa                                                                                     <  1:268003‑M2/64‑1 (MQ=255)                                                                cgaaaccgctgattgatgcctatcttctcgccctcaatcagtggccggtgaccaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGCACGGTCTCAACCGACTGAAAGGCGCGGCGGCACAAGCGGTACa                                                                                     <  1:224156‑M2/64‑1 (MQ=255)                                                                    accgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTgc                                                                                 >  1:462053‑M2/82‑149 (MQ=255)                                                                             tgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATAtt                                                                        >  1:925896‑M2/73‑149 (MQ=255)                                                                             tgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATAtt                                                                        >  1:608869‑M2/73‑149 (MQ=255)                                                                                         tcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCg                                                                 <  2:418872‑M2/84‑1 (MQ=255)                                                                                            tgactcctatcagtggccggtgatcaaccccaccacgggcgtgggcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCt                                                              <  2:124483‑M2/87‑1 (MQ=255)                                                                                               cctccatcaggggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATCATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATg                                                           <  2:113610‑M2/90‑1 (MQ=255)                                                                                                ctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGAACGAAGACAACTTACTGAAAGGCGCGGCGGCACCAGCGGTGCAGTGCGCCAATATGGCTTGCGGCTAtgg                                                          >  2:32895‑M2/54‑143 (MQ=255)                                                                                                 tcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAAc                                                    >  1:603677‑M2/53‑149 (MQ=255)                                                                                                 tcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGAGCGGCGGCACAACCGGTACAGCCGGCCAGTCCCCGTGTCGGCTatgcggggag                                                    >  1:398019‑M2/53‑145 (MQ=255)                                                                                                 ccagtcggggtccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACCGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCg                                                         <  2:537209‑M2/92‑1 (MQ=255)                                                                                                  caatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCgg                                                        <  2:472433‑M2/93‑1 (MQ=255)                                                                                                         tggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACGTAGTGAAGGGCAGGACGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCCGtctc                                            >  1:445666‑M2/45‑149 (MQ=255)                                                                                                            ccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTa                                         >  1:537443‑M2/42‑149 (MQ=255)                                                                                                             cggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAACATGCGGTTCGGCTATGCGGAAACTCAgtcg                                             >  2:472095‑M2/41‑143 (MQ=255)                                                                                                                 gatcaaggcgaccagcggggtgagcggtgcggggcgAGCGTCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTa                                         <  2:56506‑M2/108‑1 (MQ=255)                                                                                                                  atcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGcggaaacgcaccactccgc                                        <  2:46886‑M2/109‑10 (MQ=255)                                                                                                                   tcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAgg                                  >  1:855183‑M2/35‑149 (MQ=255)                                                                                                                   tcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGAGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAgg                                  >  1:663489‑M2/35‑149 (MQ=255)                                                                                                                     aacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGACAATACTCGGTTCGGCTATGCGGAAACGCAGCCtcctactga                                     >  2:506430‑M2/33‑140 (MQ=255)                                                                                                                     aacgccaccagcggcgtgagcggtgaagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTGCGGCTCTGCGGAACCGCAGCCCCTTCTTTAAGGGt                                >  1:554164‑M2/33‑149 (MQ=255)                                                                                                                        gccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACACGCGGTGCAGTGCGCCAATATTCGTTTCGGCTATGAGGAAAAGCAGTCTCTTATTTAAGGGGGCa                             >  1:13679‑M2/30‑149 (MQ=255)                                                                                                                        gccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGgtgga                             >  1:273686‑M2/30‑147 (MQ=255)                                                                                                                         ccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCaa                            >  1:802408‑M2/29‑149 (MQ=255)                                                                                                                                     tgagcggttcagggcgAGGATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCat                <  1:59077‑M2/133‑1 (MQ=255)                                                                                                                                        gcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATCTTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCattaa             <  1:872792‑M2/136‑1 (MQ=255)                                                                                                                                         cggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATgaatacc                 >  2:368220‑M2/13‑141 (MQ=255)                                                                                                                                          ggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCat                <  2:521307‑M2/133‑1 (MQ=255)                                                                                                                                            tgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGCAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAAGTAt         >  1:767613‑M2/10‑149 (MQ=255)                                                                                                                                              cagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCa       >  1:529659‑M2/8‑149 (MQ=255)                                                                                                                                                 ggcgGGCATCTGATTATTGTGGCGACCGAAGACAATTTACAGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTAt         <  2:88714‑M2/140‑1 (MQ=255)                                                                                                                                                   cgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGTCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCCTTAATTATCAAACTg  >  1:627512‑M2/3‑149 (MQ=255)                                                                                                                                                     aGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTCTCcaa     <  2:784714/144‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TCGGGTGTGACCCAGGCGCAAGTCGCGCAAGTGTTACAGCAGGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTG  >  CP009273/4145673‑4145967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑