Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,919,680 C→T A46T (GCC→ACC)  mioC ← FMN‑binding protein MioC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,919,6800CT97.5% 378.4 / NA 120A46T (GCC→ACC) mioCFMN‑binding protein MioC
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base T (66/49);  minor base G (0/3);  total (66/54)
Fisher's exact test for biased strand distribution p-value = 8.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCA  >  CP009273/3919539‑3919824
                                                                                                                                             |                                                                                                                                                
    gCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGAGTGGCGCTGTTAACCAGCCAGATCCCTGAGGTAGGTAAAtctt                                                                                                                                       >  2:1842249/1‑149 (MQ=255)
     ccAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAAtcttc                                                                                                                                      >  2:1882973/1‑149 (MQ=255)
       caTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTa                                                                                                                                    <  2:1519867/148‑1 (MQ=255)
        aTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTaa                                                                                                                                   <  2:820706/149‑1 (MQ=255)
         tACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAAtctt                                                                                                                                       <  1:89977/144‑1 (MQ=255)
          gccGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAAtcttc                                                                                                                                      <  1:512210/143‑1 (MQ=255)
          aCCGAAAGCGCCAAAGCGGCATGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAAtcttc                                                                                                                                      >  1:1304889/1‑144 (MQ=255)
            cGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCCATGCTTCATAGAAAGGGGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGc                                                                                                                               >  2:778664/1‑149 (MQ=255)
               ttGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGa                                                                                                                            <  2:857814/149‑1 (MQ=255)
                   gcCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGt                                                                                                                        <  2:874836/149‑1 (MQ=255)
                    ccAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCTCCGTGGTTGTAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGa                                                                                                                            <  1:559894/144‑1 (MQ=255)
                     cAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCTATGCTTCAACGCAAGGAGAAAGGTTGTCCGGAGTATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCGTATCCCTGAGGTAGGTAAATCTTCTAGCCGAGGAc                                                                                                                           >  1:188077/1‑144 (MQ=255)
                     cAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGAc                                                                                                                           >  1:106746/1‑144 (MQ=255)
                     cAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGc                                                                                                                      >  2:884360/1‑149 (MQ=255)
                        aGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGc                                                                                                                   <  2:1643310/149‑1 (MQ=255)
                          cGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGt                                                                                                                 >  2:1828664/1‑149 (MQ=255)
                           ggACTGCAGAAAGATCGGGCTTCTGTGCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGGGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGtt                                                                                                                <  2:576750/149‑1 (MQ=255)
                            gACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGttt                                                                                                               <  2:709709/149‑1 (MQ=255)
                            gACTGCAGAAAGATCGGGCCTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGttt                                                                                                               <  2:709372/149‑1 (MQ=255)
                                gCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGGAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggt                                                                                                           <  2:442369/149‑1 (MQ=255)
                                    aaaGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTa                                                                                                       <  2:1074688/149‑1 (MQ=255)
                                    aaaGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTa                                                                                                       >  2:1823622/1‑149 (MQ=255)
                                     aaGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggt                                                                                                           <  1:1322932/144‑1 (MQ=255)
                                     aaGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggt                                                                                                           <  1:1650663/144‑1 (MQ=255)
                                       gATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTaaaa                                                                                                    <  2:431144/149‑1 (MQ=255)
                                        aTCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggtggt                                                                                                        >  1:563761/1‑144 (MQ=255)
                                        aTCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCgggggt                                                                                                        >  1:1122208/1‑144 (MQ=255)
                                         tCGGGCTTCTGTGCCCGCAATGCTTCATATAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGACAACCAGCCAGGTCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTa                                                                                                       <  1:388257/144‑1 (MQ=255)
                                               ttCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGCTCTTGGAGGTAGTGAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCctc                                                                                            >  2:953410/1‑149 (MQ=255)
                                               ttCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCctc                                                                                            >  2:1470075/1‑149 (MQ=255)
                                               ttCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCctc                                                                                            >  2:1227063/1‑149 (MQ=255)
                                                  tGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTc                                                                                         <  2:992225/149‑1 (MQ=255)
                                                   gTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCct                                                                                             >  1:1175102/1‑144 (MQ=255)
                                                    ttCCTGCAATGCTTTATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCa                                                                                       <  2:1660573/149‑1 (MQ=255)
                                                    ttCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCa                                                                                       >  2:1323674/1‑149 (MQ=255)
                                                      ccTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGTATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGTGTTTCGGTTGTAAAACCCGCCTCTTCCAGc                                                                                     <  2:1039435/149‑1 (MQ=255)
                                                       caactaTGCTTCATAGATGGGTGGAGGGTTGTCCGTAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTc                                                                                         >  1:1536347/6‑144 (MQ=255)
                                                       cTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGTAATATCTCCGGCACCGGGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTc                                                                                         >  1:1536175/1‑144 (MQ=255)
                                                        tGCAATGCTTCATAGAAAGGAGAACGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAACACCGCCTCTTCCAGCtt                                                                                   >  2:195093/1‑149 (MQ=255)
                                                         gCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGGAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCTGCCCCTTCCAGCttt                                                                                  >  2:1295225/1‑149 (MQ=255)
                                                              gCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGc                                                                             <  2:1129054/149‑1 (MQ=255)
                                                                ttCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCCCTTCGAGCTTTTCAGCCa                                                                           >  2:1974407/1‑149 (MQ=255)
                                                                  cATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAg                                                                              >  1:1363084/1‑144 (MQ=255)
                                                                  cATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAAGCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAg                                                                              >  1:1363112/1‑144 (MQ=255)
                                                                       aaaggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAgg                                                                         >  1:1313832/1‑144 (MQ=255)
                                                                         aggagaaaggTTGGCCGGAATATCTCCGGCACCGTGGGTTGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTACATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTg                                                                  <  2:752879/149‑1 (MQ=255)
                                                                          ggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACGCGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGc                                                                 <  2:1537953/149‑1 (MQ=255)
                                                                          ggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGc                                                                 >  2:1187371/1‑149 (MQ=255)
                                                                              aaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCCGCTTTTCAGCCAGGCGTTCTg                                                                  >  1:817775/1‑144 (MQ=255)
                                                                              aaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTg                                                                  >  1:1638915/1‑144 (MQ=255)
                                                                                  gTTGTCCGGAATATTTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGCTCCCTGAGGTAGGTAAATCTTCTAACAGCTGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATAtt                                                         <  2:770722/149‑1 (MQ=255)
                                                                                  gTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTAc                                                              >  1:1611094/1‑144 (MQ=255)
                                                                                  gTTGTCCGGAATATCTCCCGCACCGTGGGTGGAGCTGATACCCGGATCGAACCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATAtt                                                         >  2:1453684/1‑149 (MQ=255)
                                                                                   ttGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTc                                                        <  2:1146177/149‑1 (MQ=255)
                                                                                   ttGTCCGGAATATCTCCGGCACCGTGGGGGGAGCTGATAACCAGCCAGCTCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGGGGTAAAACCCGCCTCTTCCCGCTTTTCAGCCAGGTGTTCTGCTACa                                                             >  1:666291/1‑144 (MQ=255)
                                                                                    tGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCg                                                       <  2:1821708/149‑1 (MQ=255)
                                                                                      tCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACatat                                                          <  1:1699136/144‑1 (MQ=255)
                                                                                      tCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCCACatat                                                          >  1:192786/1‑144 (MQ=255)
                                                                                        cGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATCTTc                                                        >  1:1004427/1‑144 (MQ=255)
                                                                                        cGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGCAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTGTTCAGCCAGGAGTTCGACTACATACTc                                                        >  1:732754/1‑144 (MQ=255)
                                                                                             tatCTCCAGCACCGTGGCTGGAGCTCATAACCAGCCACATCCCTGAGGTAGGGAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCAccgccg                                              <  2:263225/149‑1 (MQ=255)
                                                                                               tctcCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAg                                            <  2:715638/149‑1 (MQ=255)
                                                                                                 tcCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATTTTCGGCAccgcc                                               >  1:456054/1‑144 (MQ=255)
                                                                                                 tcCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCAccgcc                                               >  1:220431/1‑144 (MQ=255)
                                                                                                 tcCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAggg                                          >  2:1477415/1‑149 (MQ=255)
                                                                                                 tcCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAggg                                          >  2:1507995/1‑149 (MQ=255)
                                                                                                  ccGGCACCGTGGGTGGAGCTGATAACCAGTCAGATCCCTGAGGTAGGTAAATCGTCTAACAGCGGACCGTGCTGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCAccgccg                                              <  1:215820/144‑1 (MQ=255)
                                                                                                  ccGGCACCGTGGGTGCAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCCTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCGGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCAccgccg                                              >  1:669844/1‑144 (MQ=255)
                                                                                                   cGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtg                                        >  2:1654919/1‑149 (MQ=255)
                                                                                                   cGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtg                                        >  2:765795/1‑149 (MQ=255)
                                                                                                    ggCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtga                                       >  2:1075057/1‑148 (MQ=255)
                                                                                                     gCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtgct                                      >  2:243829/1‑149 (MQ=255)
                                                                                                      cACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtgctg                                     <  2:1715969/149‑1 (MQ=255)
                                                                                                          gtgggtggAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCATTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCCCCGAGGGTGCTGCGGc                                 >  2:1199836/1‑149 (MQ=255)
                                                                                                           tggggggAGCTGGAACCCGCCCAGGTCCCTGAGGGAGGTAAATCTTCTACCAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACAGCCGAggttgttg                                     <  1:1685523/144‑7 (MQ=255)
                                                                                                             gggggAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGa                              <  2:131799/146‑1 (MQ=255)
                                                                                                              gtggGGCTCAGAACCAGCCAGAGCGCTGAGGTAGGTAAATCTCCTAACAGCGGACCGTGCGGCGGTTCGGTGGTAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCg                                  <  1:781839/144‑1 (MQ=255)
                                                                                                              gtggAGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACCTATTCGGCCCCGCCGAGGGTGCTGCCg                                  >  1:730991/1‑144 (MQ=255)
                                                                                                                ggcactggacaCCAGCACGATCCCGGATGTAGGCAACCCGTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTCTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCt                                <  1:115605/134‑1 (MQ=255)
                                                                                                                  accTGATACCCGTCCAGATCCCTGAGGCAGGTAAATCTCCTACCAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAaga                         <  2:373420/147‑1 (MQ=255)
                                                                                                                  aGCTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACACATCCGGCACCGCCGAGGGTGCTGCCGCTGATAaga                         <  2:1961080/149‑1 (MQ=255)
                                                                                                                   gcggacaaCCAGCCAGACCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGGGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGAt                             <  1:446127/138‑1 (MQ=255)
                                                                                                                    cTGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGt                       >  2:33768/1‑149 (MQ=255)
                                                                                                                     tGATAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGGGTg                      >  2:1847753/1‑149 (MQ=255)
                                                                                                                       aTAACCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAaga                         >  1:201606/1‑144 (MQ=255)
                                                                                                                        tAACCAGCCAGAGCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGata                   <  2:124298/149‑1 (MQ=255)
                                                                                                                         aaCCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGTACCGCCGAGGGTGCTGCCGCTGATAAGAGTGatct                  >  2:316033/1‑147 (MQ=255)
                                                                                                                         aaCCAGCCAGATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCGGCCGCTGATAAGAGt                       >  1:1591863/1‑144 (MQ=255)
                                                                                                                            cagccagTTCCCTGAGGTAGGTAAATCTTCTTACCGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGat                    <  1:30684/144‑1 (MQ=255)
                                                                                                                            cagccagATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTggt                    >  1:1696805/1‑142 (MQ=255)
                                                                                                                            cagccagATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGat                    >  1:1689299/1‑144 (MQ=255)
                                                                                                                            cagccagATCCCTGAGGTAGGTAAAGCTACTAACAGCGGACCGTGCAACGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTg               >  2:1113458/1‑149 (MQ=255)
                                                                                                                              gccagATCCTTGACGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGcc             <  2:205012/149‑1 (MQ=255)
                                                                                                                              gccagATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCCGGTGTTCTGCTACATATTCGGCACAGCCGGGGGTGCTGCCGCTGATAAGAGTGATCTCTGcc             >  2:605578/1‑149 (MQ=255)
                                                                                                                                  ggtcgcggAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGc              <  1:1751425/137‑1 (MQ=255)
                                                                                                                                  gATCGCTGAGGGAGGTAAGTCCTGTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATCCGCCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGc              <  1:1677753/144‑1 (MQ=255)
                                                                                                                                  gATCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGGTAGGAGTGGTATCTGGCATaa         >  2:568977/1‑149 (MQ=255)
                                                                                                                                   aTCCCTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATaaa        >  2:574067/1‑149 (MQ=255)
                                                                                                                                     cccTGAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCAt           >  1:154581/1‑144 (MQ=255)
                                                                                                                                         gAGGTAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGGGCAACCGTTGGTGATGCCGCTGCTAAGAGTGATATCTGCCATAAACCGCCa  >  2:1933058/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCA  >  CP009273/3919539‑3919824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑