Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP009273 | 777,282 | A→T | 6.8% | intergenic (+174/‑259) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase, subunit A |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 777,282 | 0 | A | T | 6.8% | 249.6 / 5.5 | 98 | intergenic (+174/‑259) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
Reads supporting (aligned to +/- strand): ref base A (39/50); new base T (3/4); total (43/55) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 |
TTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGA > CP009273/777134‑777422 | ttttttGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTa > 1:943764/1‑149 (MQ=32) tttttGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa < 1:634999/149‑1 (MQ=32) tATCTGCGATACTCAAAGATTCGAACCTGCCGCAGGGTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCT > 2:692105‑M1/1‑149 (MQ=255) tATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGTGAGTAATCCTGCTCGCACCACCAATGTAAAAAAGCAACCTAAAAGCGCT > 1:165751‑M1/1‑149 (MQ=255) tATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATGTAAACAAGCCCCCTAAAGGCGCT > 1:45069‑M1/1‑149 (MQ=255) tATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCT > 1:1114198‑M1/1‑149 (MQ=255) aTCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTT > 2:207219‑M1/1‑149 (MQ=255) tCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTT < 1:1217546‑M1/149‑1 (MQ=255) tCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCTTT > 2:1063447‑M1/1‑149 (MQ=255) tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT > 1:938576‑M1/1‑149 (MQ=255) tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT > 2:1005570‑M1/1‑149 (MQ=255) tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT > 2:194382‑M1/1‑149 (MQ=255) tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT > 1:162782‑M1/1‑149 (MQ=255) gCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTT < 2:32258‑M1/149‑1 (MQ=255) gCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCCTATTT > 2:935768‑M1/1‑149 (MQ=255) tCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGACATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGCCCCTTTTTTACTATTc > 2:383892/1‑149 (MQ=9) cAAAGATTCGCACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGCCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa < 1:289496/149‑1 (MQ=12) cAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa < 2:1023133/149‑1 (MQ=32) cAAACATTCGCAGCTGCAGCAGGTTTTAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa < 1:1090575/149‑1 (MQ=255) aaaGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAg > 2:385073/1‑149 (MQ=32) tttgtaCCCGCAGCAGGTTTGGGTTGAGCGCAGGGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc < 2:314910/144‑1 (MQ=255) ttCGAACCTGACGGAGGTTTGAGTTGAGCGCAGCCAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACTCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc < 2:815303/149‑1 (MQ=11) tcccaaCCTACAGCAATTTTCAGTCGAGCGCACCGAAACAACGGAGCCCCACGCGGCCACGGCCCAAAGAGCGATCGAAGCTAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCATGCATc < 2:521586/145‑1 (MQ=11) gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTc > 1:1209755/1‑149 (MQ=39) gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCAGCCTc > 1:628386/1‑149 (MQ=37) tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAAttt < 2:922917/149‑1 (MQ=255) cgcagGTTTGGGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAc < 1:249039/148‑1 (MQ=255) agcagTTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCCAAAGGCGCTTTTTTACTATTCAGGCATCCTCAATTTCAc > 2:868637/1‑149 (MQ=255) cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAGTTTCACtt > 1:194346/1‑149 (MQ=255) cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACtt > 1:1117339/1‑149 (MQ=255) ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgc < 1:1063447‑M1/149‑30 (MQ=255) ttgagttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTaa > 1:319575/1‑149 (MQ=255) gggttgggCGCGGGGAAACAACGGAGCCGGTCAGGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCTCCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc < 2:697290/147‑1 (MQ=17) gggttgagCGCCGCGCAACAAGGGCGCCGCTCGCGGCGACGGCTCGAACGGCGAGCGACGCGTCTATGCCTGTATTACCCACCAATGTAAAAAAGCGCCCTAACCGCGCTTTCTTGCTATTCAGGCATCCTTAATTTCACtttttccac < 2:571627/147‑7 (MQ=9) gagttgcgCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc < 2:735217/149‑1 (MQ=255) gagttgagCGCCGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGAGCGAGCGAAGCGACTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc < 2:886736/149‑1 (MQ=255) gagttgagCGCAGCGAAACAACGGCGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc < 2:894774/149‑1 (MQ=255) agttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgcttcgct < 2:1103703‑M1/149‑36 (MQ=255) gttgagCGCAGCGAAACAACGGAGCCGCTAGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCt > 1:556480/1‑149 (MQ=255) ggtgagcgGAGCGAAGCAAGGGGGCCGGTCGCGGCGACGGCCCGATGGGCGGGGGAGGCGAGTCATCCTGCAGGACCCACGTATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgcttcgctc < 2:1084301‑M1/147‑37 (MQ=255) ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGTGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg > 1:550191/1‑149 (MQ=255) ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTAACTTTGTAAACCtg > 1:582197/1‑149 (MQ=255) ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCCCTTTGTCAACCtc > 1:618843/1‑148 (MQ=255) ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg > 1:489856/1‑149 (MQ=255) ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg > 1:458886/1‑149 (MQ=255) tgagCTCCGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGTGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGCAAAAACGCGCCTTTAGGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgcttcgctcgc < 1:1121106‑M1/149‑39 (MQ=255) gagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgat < 2:331148/149‑1 (MQ=255) gcgcAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgctga > 1:103728/1‑149 (MQ=255) cgcAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGAc > 1:1128919/1‑149 (MQ=255) gcAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACa < 2:497805/149‑1 (MQ=255) caacaccGGAGCCGCTCGTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGCCa < 2:1038066/148‑1 (MQ=255) acaacGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCaga < 1:385073/149‑1 (MQ=255) caacGGTGCCGCTCGCGGCGGCGGACCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCagag < 1:553121/149‑1 (MQ=255) caacGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCagag < 2:336256/149‑1 (MQ=255) ggAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACCTTGTAAACCTGATGACATCGTCAGAGCTTa < 1:650342/149‑1 (MQ=255) ggAGCCGCCCGCGGCGGCGCCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACCGCCCTTTGTTTCTATTATGGGATCCTCAATTTCACTTTGTAAACCTTATGACATCGTCAGAGCTTa < 1:601591/149‑1 (MQ=18) gtgCCGTTCGCGGCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTAc < 2:173923/147‑1 (MQ=255) gAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTAc > 1:983307/1‑149 (MQ=255) aGCCGCTCGCGGCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGAGGACATCGTCAGTGCTTACt < 2:843255/149‑1 (MQ=255) tCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaa < 2:642446/149‑1 (MQ=255) cgcgGCGACGGCCCGAAGGGCGAGCGAAGCTAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag < 1:500528/149‑1 (MQ=255) cggcgACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagca > 1:1156430/1‑149 (MQ=255) cgACGGCCCGAAGGGCGAGCTAAACTACTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAAct < 2:1178495/149‑1 (MQ=255) ccGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTCAAGGCGCTTTTTTGCTATTCCGGAATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAAGCCTTACTGTGCAAGCAACTCTATGTc > 1:289881/1‑149 (MQ=25) cGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCg < 2:372798/149‑1 (MQ=255) gAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCgg < 1:655541/149‑1 (MQ=255) aaGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGt < 2:279349/149‑1 (MQ=255) aGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTg < 2:559777/149‑1 (MQ=255) gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa < 1:328722/149‑1 (MQ=255) gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa > 2:1154635/1‑149 (MQ=255) gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa < 2:556480/149‑1 (MQ=255) gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGATATTCAGGCATCCTCAATTTACCTTTGTAAACCTGATCAAATCGTCAAAGCTTCCTGTGACGGCAAATCTATGTCGGTGGaa > 1:723852/1‑149 (MQ=21) gcgagcgaAGCGAGTCATCCGGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTCTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa < 2:556511/149‑1 (MQ=255) cgagggaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAAt < 2:1209811/149‑1 (MQ=255) gagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACACTCTGTCGCTGGAAtt > 2:730613/1‑149 (MQ=255) agcgaagcgaGTCATCCTGCACGACCCACCCCTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCTTCCCCAAGTTCACTTTGTAAACCTCATGAACTGGTCAGTGCTTACTGTGCAAGCAACTCTATGTCGATGGCAtga > 2:619741/1‑147 (MQ=21) cgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAGCGCTAAGTCCGGGGAATTAgg > 2:967992/1‑149 (MQ=37) cgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCCTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAgg < 2:619935/149‑1 (MQ=255) tCATCCTGCACGACCCACCAATGTACCAAGGAAACTTTTAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAATTTGTAAACCTGATGAAATCGTCAGAGCGTACTGTGCAACCAGCTCTCCGTCGGTGTGAGTAGGCGTAAAATg > 1:299396/1‑149 (MQ=255) tCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTATACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTGGGCGTAAAATg < 2:147059/149‑1 (MQ=255) cATCCTGCACGACCCGCCAATGTAATAAAGCGCCCTTAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGa < 1:748721/149‑1 (MQ=255) cATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGa < 1:202515/149‑1 (MQ=255) aTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGAc < 2:1209755/149‑1 (MQ=255) tCCTGCACTACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACg < 1:1004232/149‑1 (MQ=255) cgtGCACGACCCACCAGTAAAAAATGTCGCCCTAATGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGc < 2:1178117/147‑1 (MQ=255) cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCa > 2:209575/1‑149 (MQ=255) ccGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACACAGTCAGAGCTTACTGTGCAAGCAACCCTATGTCCGTGGAATTAGGCGTAAAATGCCggacccc > 2:40366/2‑143 (MQ=38) aCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCt < 1:1085687/149‑1 (MQ=255) aCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGATTACCTTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCt < 1:659528/149‑1 (MQ=255) aCGACCCACCAAGGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCt < 1:5844/149‑1 (MQ=255) gACCCACCAATGTAAAAAAACGACCTAAAGGCGCTGTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCCGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGc > 2:912603/1‑149 (MQ=255) accaATGTAAAAAAGCGCCCTAAAGGCGCGTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACAtt < 2:491072/149‑1 (MQ=255) accaATGGAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTAATCAGGCATCCTCAATTTCCCTTTGTAAACCTGAAGACATCGTCAGAGCTTACTGTTCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACAtt < 1:21717/149‑1 (MQ=255) aTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGc < 1:271950/149‑1 (MQ=255) tAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCGTCCTGCACATTAGGCGTa > 1:93544/1‑149 (MQ=255) tAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTa > 1:93388/1‑149 (MQ=255) aaaaaGCGCCTTTAGGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt > 2:814983/1‑149 (MQ=255) aatccacccTGAAGGCGCTTTTTTGCTATTCAGGCATCCTCAACTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACAGTAGGCGTAATTc < 2:237486/143‑1 (MQ=255) aaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTc < 1:880263/149‑1 (MQ=255) aGCGCCCTAAAGGCGCTTTTTTacattggtgggtcgtgtaggatgactcgcttcgctcgcccttcgggccgtcgccgcgagcggctccgttgtttcgctgcgctcaactcaaacctgctgcaggttcgaatcttttagtatcgcagata > 2:267227‑M1/1‑22 (MQ=255) | TTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGA > CP009273/777134‑777422 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |