Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 903,388 A→G 4.1% H117H (CAT→CAC ybjT ← putative NAD‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273903,3880AG 4.1% 267.2 / 2.8 99H117H (CAT→CACybjTputative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (47/46);  new base G (2/2);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-02

AGGTTGTAGACCATATCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTA  >  CP009273/903242‑903535
                                                                                                                                                  |                                                                                                                                                    
aGGTTGTAGACCATATCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGTTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACGCCTCAtg                                                                                                                                                    >  1:239623/1‑149 (MQ=255)
   ttGTAGACCATACCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTgg                                                                                                                                                 <  1:931269/149‑1 (MQ=255)
    tGTAGACCATATCGCGCATGACTTCGGACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGc                                                                                                                                                <  2:829194/149‑1 (MQ=255)
     gcaGACCAGATCGCGCATGACTTCGAACGCCTGGGAACCTGCGCCAACGACAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCGTCACGCAGAATGTCCCATGGTGCGAGTCGAGCACGCAGATGATCCGACTGCTCATGTGGCg                                                                                                                                               <  1:297416/147‑1 (MQ=255)
     gTAGACCCTATCGCGCATGACTTCGACCGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCg                                                                                                                                               <  1:734578/149‑1 (MQ=255)
     gTAGACCATATCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCgg                                                                                                                                              <  1:264990/149‑1 (MQ=255)
      tAGACCATATCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTGGCCTGACGAGCACGCAGATGATCCGGCTGCTCATGTGGCgg                                                                                                                                              >  1:87242/1‑149 (MQ=255)
         aCCATATCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTGGGCGGACGAGGACGCAGATGATCCGACTGCCCATGTGGCGGGGc                                                                                                                                          >  1:342236/1‑149 (MQ=255)
           ccttgtgcggATGACTGCGACCGCCGCGGCACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACCCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCTACTGCTCATGTGGCGGGGccc                                                                                                                                        <  2:433990/143‑2 (MQ=255)
           cctagcgctCATGACTTCGAACGCCGCGGAACCCGCGCCAACGATAATTCCGTCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACTAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGccc                                                                                                                                        <  1:279235/144‑2 (MQ=255)
             tctCGCGCAGGACTTCAAGCTGCCCAGAGCCGGCGCCAACGATAATTCCGGCCCGGAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGGCTGCTCATGTGGCGGGGCCTGc                                                                                                                                      <  2:562481/147‑1 (MQ=255)
             tatCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGc                                                                                                                                      <  2:739029/149‑1 (MQ=255)
               tCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCGGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCaa                                                                                                                                    >  1:197508/1‑149 (MQ=255)
               tCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCaa                                                                                                                                    <  1:1121405/149‑1 (MQ=255)
                  cgcATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCCCGCAGCTGATCCCACTGCTCATGTGGCGGGGCCTGCAActa                                                                                                                                 >  1:427673/1‑147 (MQ=255)
                  cgcATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGa                                                                                                                                 <  2:257310/149‑1 (MQ=255)
                  cgcATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCAGCCGTTGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGa                                                                                                                                 >  1:1212618/1‑149 (MQ=255)
                      tGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGTATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTa                                                                                                                             <  1:81955/149‑1 (MQ=255)
                      tGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTa                                                                                                                             >  1:752407/1‑149 (MQ=255)
                      tGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGCCGGGGCCTGCAACGAACTa                                                                                                                             >  1:158446/1‑149 (MQ=255)
                         cTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTaaga                                                                                                                          >  1:1017906/1‑149 (MQ=255)
                          ttCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCCACGAACTaagaa                                                                                                                         >  1:609513/1‑149 (MQ=255)
                            cGAACGCCGCGGAACCTGCGCCAACGATAGTTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGGCGAGCACGCAGATGATCCGACTGCTCCTGTGACGGGGCCTGGAACGAACTaaggaag                                                                                                                       >  1:753158/1‑149 (MQ=255)
                             gAACGCCGCGGAAACTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTaagaaaga                                                                                                                      >  2:756220/1‑149 (MQ=255)
                               aCGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTGATGTGGCGGGTCCTGCAACGAACTAAGAAGGAtt                                                                                                                    >  2:508237/1‑149 (MQ=255)
                                  gagcgcAACCTGCGTCAACGATAAGTCCGGCCCGCAGCTCGGTCACGGGGACAGTAGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGAGGATCCTAATGCTCATGTGGCGGGGGCTGCAACGAACTAAGAAAGATTAAt                                                                                                                 <  2:623890/147‑1 (MQ=255)
                                     cgGAACCTGCGCCAACGATGATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGc                                                                                                              <  1:702572/149‑1 (MQ=255)
                                     cgGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAAGGAACTAAGAAAGATTAATTGc                                                                                                              >  2:412555/1‑149 (MQ=255)
                                     cgGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGc                                                                                                              <  2:122176/149‑1 (MQ=255)
                                     cgGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAACCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGGACTCAGAAAGATTAAtttg                                                                                                              >  1:223528/1‑147 (MQ=255)
                                         aCCTGGGCCAACAATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTaa                                                                                                          <  2:309367/149‑1 (MQ=255)
                                           cTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACAGTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACt                                                                                                        >  2:572995/1‑149 (MQ=255)
                                            tGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGACGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGACCCAAGGAAGATTAATTGCTTAAcgg                                                                                                       >  1:721295/1‑147 (MQ=255)
                                             gcgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCCTGTGGCGGGGCCTGCAACGAACTAAGAAAGATTGATTGCTTAACTgg                                                                                                      >  1:772045/1‑149 (MQ=255)
                                             gcgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTgg                                                                                                      <  2:863410/149‑1 (MQ=255)
                                             gcgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTgg                                                                                                      <  1:139031/149‑1 (MQ=255)
                                             gcgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTgg                                                                                                      <  1:886170/149‑1 (MQ=255)
                                             gcgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGAACCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTgg                                                                                                      >  2:807438/1‑149 (MQ=255)
                                             gcgcCAACGATAATTCCGGCCCGCAGTTCGGCCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTgg                                                                                                      >  2:162917/1‑149 (MQ=255)
                                              cgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGt                                                                                                     >  2:904578/1‑149 (MQ=255)
                                              cgcCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAAATTAATTGCTTGACTGGt                                                                                                     >  1:469453/1‑149 (MQ=255)
                                                  aaCGATAATTCCGGCCCGAAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACtt                                                                                                 >  2:799995/1‑149 (MQ=255)
                                                     gATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCAc                                                                                              >  1:1155280/1‑149 (MQ=255)
                                                      aTAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACg                                                                                             <  1:974751/149‑1 (MQ=255)
                                                      aTAATTCCGGCCCGCAGTTCGGTCACGGGTACATTAGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACg                                                                                             <  1:692362/149‑1 (MQ=255)
                                                      aTAATTCCGGCCCGCAGTTCGGGCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACg                                                                                             <  1:526890/149‑1 (MQ=255)
                                                          ttCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAgc                                                                                         >  2:483766/1‑149 (MQ=255)
                                                              ggCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCaa                                                                                     >  2:134885/1‑148 (MQ=255)
                                                                  cGCAGTTCGGTGACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATcgcg                                                                                 <  2:491553/149‑1 (MQ=255)
                                                                  cGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCCCGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATcgcg                                                                                 >  2:560241/1‑149 (MQ=255)
                                                                   gCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCCTCGCgg                                                                                >  2:554591/1‑149 (MQ=255)
                                                                    cAGTTCGGTCACTGGCACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCATGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGa                                                                               <  1:501001/149‑1 (MQ=255)
                                                                    cAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGa                                                                               <  1:586129/149‑1 (MQ=255)
                                                                         cGGTCACGTGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTg                                                                          <  2:238127/149‑1 (MQ=255)
                                                                         cGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATTCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCACATCGCGGACGTTg                                                                          <  2:1056299/149‑1 (MQ=255)
                                                                           gTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCGTGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTgag                                                                        >  2:1107735/1‑149 (MQ=255)
                                                                           gTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCGTGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTgag                                                                        >  2:934069/1‑149 (MQ=255)
                                                                           gTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTgag                                                                        <  1:1221981/149‑1 (MQ=255)
                                                                           gTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTgag                                                                        >  2:815284/1‑149 (MQ=255)
                                                                           gTCACGGGTACATTCGCTTCACGCAGAATGTACCTGGTGGTGTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATGGCGGGCGTTgag                                                                        >  2:575056/1‑149 (MQ=255)
                                                                            tCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCACCGCGGCCGTTggga                                                                       >  2:39109/1‑149 (MQ=255)
                                                                                  gTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCAcc                                                                 <  1:508237/149‑1 (MQ=255)
                                                                                      aTTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTAATGTGGCGGGGCCTGCAACGAACTCCGGAAGATTAATCGGTTAACTGGTACTTCACGTAGCGCACCGCGGGCGTAGAGGGCGACCCGgc                                                             >  2:961794/1‑149 (MQ=255)
                                                                                         cGCTTCGCGCAGAATGGCCGCGGGAGCCGGACGAGCCAGCACATGCACCACCTGCTCAGGTGGCGGCGGCAGGCAGCACGTGGGTATGGTTAGGGGCTTCACTGGTACTTCCCGTAGCGCATCGCGGACGTTGAGCGCCAGGTGGctct                                                          <  2:810361/149‑1 (MQ=255)
                                                                                          gCTTCACGCATAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATGAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTc                                                         <  1:930830/149‑1 (MQ=255)
                                                                                           cTTCACGCAGAATGTACGAAGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCAGACGTTGAGAGCCACCTGGCGCTca                                                        >  1:911098/1‑148 (MQ=255)
                                                                                               aCGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCCCCTGAg                                                    >  1:279281/1‑149 (MQ=255)
                                                                                               aCGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGGACTTCACGGAGCACATCGCGGACGTGGAGAGCCACCTGGCGCTCCGGAg                                                    >  1:871506/1‑149 (MQ=255)
                                                                                                 gCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGGTTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGGGCg                                                  >  1:118933/1‑149 (MQ=255)
                                                                                                         tCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATc                                          >  2:839124/1‑149 (MQ=255)
                                                                                                                  gCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAAGCGCCGCCTTc                                 >  1:436348/1‑149 (MQ=255)
                                                                                                                   ccTGCCGATCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCg                                <  2:390166/149‑1 (MQ=255)
                                                                                                                     tGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACTTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGcc                              <  1:799995/149‑1 (MQ=255)
                                                                                                                     tGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGcc                              <  2:1017906/149‑1 (MQ=255)
                                                                                                                     tGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGcc                              <  1:1103067/149‑1 (MQ=255)
                                                                                                                     tGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGcc                              <  1:1102940/149‑1 (MQ=255)
                                                                                                                           gCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAAAGGCCCAACAAAGATTAACTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGGTAAAATCGCCGCCTTCGCCCATGCt                        >  1:1220069/1‑149 (MQ=255)
                                                                                                                            cACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGCGAGACATCTGGGCGTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCtg                       >  2:566407/1‑149 (MQ=255)
                                                                                                                             aCGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCtgt                      <  2:593142/149‑1 (MQ=255)
                                                                                                                             aCGCAGATGATCCGACTGCTCATGGGGCGGCGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCtgt                      <  2:343261/149‑1 (MQ=255)
                                                                                                                              cGCAGATGATCCGACTGCTCGTGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCtgtg                     <  1:1107735/149‑1 (MQ=255)
                                                                                                                              cGCAGATGATCCGACTGCTCGTGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCtgtg                     <  1:934069/149‑1 (MQ=255)
                                                                                                                               gCAGAGGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGGTGAAACCGCCGCCTTCGCCCATCCCGTGc                    >  2:1074064/1‑149 (MQ=255)
                                                                                                                                cAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAAATGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCa                   <  1:839578/149‑1 (MQ=255)
                                                                                                                                cAGATGACCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCa                   <  1:747977/149‑1 (MQ=255)
                                                                                                                                 aGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCAc                  <  2:338690/149‑1 (MQ=255)
                                                                                                                                  gatgatCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATACAATCGCCGCCTTCCCCCATGCTCGGCAcc                 >  2:1117418/1‑149 (MQ=255)
                                                                                                                                  gatgatCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCAcc                 <  2:158446/149‑1 (MQ=255)
                                                                                                                                   atgatCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCa                >  2:301592/1‑149 (MQ=255)
                                                                                                                                   atgatCCGACTGCGCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCa                >  1:253809/1‑149 (MQ=255)
                                                                                                                                      atCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGCAAGAGTAATTGCTTAACTGGGACTTCACGTAGCGCATCGCGGACGTGGAGAGGCAACAGGAGCTCCTGAGCGATAAAACCGCCGCCTTCGCCCATGCCGTGCACCAGaa             >  2:504834/1‑149 (MQ=255)
                                                                                                                                      atCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGaa             >  2:657801/1‑149 (MQ=255)
                                                                                                                                      atCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGaa             <  2:353581/149‑1 (MQ=255)
                                                                                                                                        ccGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGaaaa           >  1:832513/1‑149 (MQ=255)
                                                                                                                                             tGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTGACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCGGGCGCTCCTGGGCGATAAAATCGCCGCATTCGCCCATGCTCGGCACCAGACAATAAAc      >  2:1014405/1‑149 (MQ=255)
                                                                                                                                             tGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGTCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGAc      <  1:807438/149‑1 (MQ=255)
                                                                                                                                             ggCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGAc      <  1:162917/148‑1 (MQ=255)
                                                                                                                                              gCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCCGAAAATAGAcc     >  1:1091818/1‑149 (MQ=255)
                                                                                                                                                 cATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCCGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTa  <  2:667806/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGGTTGTAGACCATATCGCGCATGACTTCGAACGCCGCGGAACCTGCGCCAACGATAATTCCGGCCCGCAGTTCGGTCACGGGTACATTCGCTTCACGCAGAATGTCCGCCGTAGCCTGACGAGCACGCAGATGATCCGACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTA  >  CP009273/903242‑903535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: