Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP009273 | 188,226 | T→C | 4.7% | intergenic (+31/‑116) | tsf → / → pyrH | translation elongation factor EF‑Ts/uridylate kinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 188,226 | 0 | T | C | 4.7% | 247.8 / 2.9 | 86 | intergenic (+31/‑116) | tsf/pyrH | translation elongation factor EF‑Ts/uridylate kinase |
Reads supporting (aligned to +/- strand): ref base T (48/33); new base C (2/2); total (50/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01 |
CTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTC > CP009273/188079‑188365 | ctgAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGcttc > 1:182686/1‑149 (MQ=255) aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttt > 1:541720/1‑149 (MQ=255) aaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGAAGCCG‑CCCTCAGGCGGCTCCtttt < 1:1094476/149‑1 (MQ=255) agagCATAACGCTGAAGTGACTGGCTCCACCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTCGAGCCTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttttt < 1:51626/149‑1 (MQ=255) agagCAAAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttttt < 2:246194/149‑1 (MQ=255) gagCATTACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTtg < 1:20170/149‑1 (MQ=255) gCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTtgtg > 2:98248/1‑149 (MQ=255) cATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGc < 1:618095/149‑1 (MQ=255) cATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGAAGCCGCCCTCA‑GGCGGCTCCTTTTtgata < 2:454996/149‑4 (MQ=38) tAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAGGGGATTGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGccc > 1:1173720/1‑149 (MQ=255) cGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGGCTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTGAATTATCACAAAGGAGCCG‑CCTGAGGGCGCCTTCTTTTTGTGCCCATc > 1:373674/1‑149 (MQ=255) tGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTg < 1:51022/149‑1 (MQ=255) cgTGACTGGTTTCAGGCGCGTTGAAGGGGGTGAACGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTaa < 1:1188003/148‑1 (MQ=255) aGTGACTGGCTTCATCCGCTTCCAAGTGGGTGAAGGCATCGATAAAGCTGAGACTGACTCTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGACTTCTTTTTGAGCCCATCTTGTaa < 2:785821/149‑1 (MQ=255) gACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAAtt > 1:174943/1‑149 (MQ=255) aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTc < 1:1141491/149‑1 (MQ=255) aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTc > 2:206037/1‑149 (MQ=255) tGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCACGCAGTGTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAg < 2:994125/149‑1 (MQ=255) tGGCGTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAg < 1:1202711/149‑1 (MQ=255) ggCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCGATCTTGTAAATTCAGc < 2:216498/149‑1 (MQ=255) ggCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGc < 1:875007/149‑1 (MQ=255) gCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCt > 2:1003972/1‑149 (MQ=255) tCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGGTGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTGTTTGTGCCCATCTTGTAAATTCAGCTAACCCt > 2:515688/1‑149 (MQ=255) tCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCt > 1:1017567/1‑149 (MQ=255) gCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgt < 1:448028/149‑1 (MQ=255) ctacGAAGGGGGTGAAGGCATCGAGAAAGTTGAGACTGTTTATGCATGAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgtg < 2:871657/146‑1 (MQ=255) cTTCGAAGTGGGTGAAGGCATCGGGAAAGTTGAGAGTGACTTTGCAGCAGAAGTTGCTGCGAAGTCCAAGCAGTCTGAATTATCAAAAAGGTGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgtg < 1:426108/149‑1 (MQ=255) cTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTACATTCAGCTAACCCTtgtg > 2:460085/1‑149 (MQ=255) cTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCAGCTTGTAAATTCAGCTAACCCTggtg > 2:277262/1‑149 (MQ=255) tGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGa > 2:239011/1‑149 (MQ=255) tGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGa > 1:1129213/1‑149 (MQ=255) ggCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTACGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAg < 1:1019819/149‑1 (MQ=255) aTCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGa > 1:1122570/1‑149 (MQ=255) tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCATGCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc > 2:421675/1‑149 (MQ=255) tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc < 1:436799/149‑1 (MQ=255) tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTCCGCTGAAAAGCGAc > 2:168959/1‑149 (MQ=255) tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGGCCAAGAAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc < 1:394119/149‑1 (MQ=255) cGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTCTCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAACTCAGCTACCCCTTGTGGGGCTGCGCTGAAAAGCGACg < 1:803843/149‑1 (MQ=255) agaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTa > 1:656970/1‑149 (MQ=255) agaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTa > 1:794385/1‑149 (MQ=255) gaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTAc > 1:494606/1‑149 (MQ=255) aaaGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACa > 1:501279/1‑149 (MQ=255) aaaGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTGAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTGCTGCCCAGCCTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACa > 2:223875/1‑149 (MQ=255) gagaCTGACTTGGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAACGTCg < 1:192611/149‑1 (MQ=255) gactgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCt > 1:460722/1‑149 (MQ=255) gactTTGCAGCAGAAGTTGCTGCGATGTCCAGGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTa > 2:1001936/1‑149 (MQ=255) gcagcaGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAAtt > 1:173906/1‑149 (MQ=255) gcagcaGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCATGTGGGGCTGCGCTGAAAAGCGACGTACAATGTTGCTAGTATTAAtt > 1:916062/1‑149 (MQ=255) gcagcaGAAGTTGCTGCGATGTCCAAGCAGGCTTAATGATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTGTTAAtt > 2:113055/1‑149 (MQ=255) gcagAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAt > 2:1008709/1‑149 (MQ=255) gcagAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAt > 1:771537/1‑149 (MQ=255) gcagAAGTTGCGGCGATGTCCAAGCAGTCTTAATTAGCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAt > 2:958281/1‑149 (MQ=255) agAAGTTGCTGCCATGTCAAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTGTTGTGCCCATCTTGGAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAATAACGACGTACTATGTCGCTAGTATTAATTCAttt < 1:299498/149‑1 (MQ=255) gTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAAt < 2:264197/149‑1 (MQ=255) ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt > 1:816413/1‑149 (MQ=255) ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt < 1:716941/149‑1 (MQ=255) gctaTGACCAAGCAGTATTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGa < 1:45615/146‑1 (MQ=255) cGATGTCCAAGCAGTCTTAATTATCAAAGAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGAc > 2:729896/1‑149 (MQ=255) cGATGTCCAAGCAGTCTTAATTATCAAAATGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGGACAATGTCGCTAGTATTAATTCATTTCAATCGTTgcc > 1:807223/1‑147 (MQ=255) cGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGAACAATGTCGCTAGTATTAATTCATTTCAATCGTTAAc > 1:116827/1‑149 (MQ=255) cAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAATCTCa > 1:93228/1‑149 (MQ=255) cAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATATTGTAAATTCAGCTAAACCTTTTGGGGCTGCGCTGAAAAGCGAGGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCa > 1:1218591/1‑149 (MQ=255) aaGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCCATCGTTGACAGTCTCAg > 1:448018/1‑149 (MQ=255) aaGCAGTCTTAATTATCAAAAAGGAGCCG‑CATGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAg > 2:386898/1‑149 (MQ=255) ctgTCTTAATTATCAAAAAGGAGCCC‑CCTGAGGGCGGCGTCTCTTTGCGCCGATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaa < 2:261553/147‑1 (MQ=255) cAGTCTTAATTATCAAAAACGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaa < 2:26126/149‑1 (MQ=255) aGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaa < 1:981673/149‑1 (MQ=255) ttAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATCAATTTCAATCGTTGACAGTCTCAGgcaaaaaa > 2:309125/1‑149 (MQ=255) aaTTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATGAATCCATTTCAATCGTTGACAGTCTCAGGAAAGAAACa < 1:667979/149‑1 (MQ=255) tATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATgg > 2:583168/1‑149 (MQ=255) aTCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGc > 2:420374/1‑149 (MQ=255) acAAAAAGAAGCCGCCCTCA‑GGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGGTGCGCCGAAAAGCGCAGTACAATGTCGCAAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCt > 1:562566/2‑149 (MQ=37) acAAAAAGAAGCCGCCCTCA‑GGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCt > 1:1237133/2‑149 (MQ=38) cAAAACGGAGCCG‑CCTGATGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTa < 1:792126/149‑1 (MQ=255) cAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTa < 1:239011/149‑1 (MQ=255) aaaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACCGTCTCGGGAAACAAACCAGGCTAc > 2:188357/1‑149 (MQ=255) aaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc > 2:505736/1‑149 (MQ=255) aaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCaa > 2:827700/1‑149 (MQ=255) aaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATGCATTCCAATCGTTGACAGTAGCAGGAAAGAAACATGGCAACCaa > 1:124745/1‑149 (MQ=255) ggAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATg < 1:984081/149‑1 (MQ=255) ccg‑ccTGAGGGCGGCTTCTTTTTGTGCCCATATTGAAAGCACTTCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCCATGCaaa > 1:605800/1‑149 (MQ=255) cttaGGGGGGCTTCTTTTTGCGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCGTTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAccc < 2:451812/146‑1 (MQ=255) ccgAGGGCGTGTTCTTTTTGGGCCCATCTTGTAAATGCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTGGTTCCTTCCAATCTTTGACAGTCTCTGGAAAGAAACATTGCTACCAATGCAAAAccc < 2:799464/147‑1 (MQ=255) tGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAATGAAACGTGGCTACCAATGCAAAACCCg > 1:861922/1‑149 (MQ=255) tGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTCCCAATGCAAAACCCg > 2:403476/1‑149 (MQ=255) gAGGGCGGCTGCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTGGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTACTTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGt < 2:232036/149‑1 (MQ=255) aGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTAGTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAGCCCGTc > 2:984504/1‑149 (MQ=255) | CTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTC > CP009273/188079‑188365 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |