Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,282,421 A→G 13.9% intergenic (+439/+122) narI → / ← tpr nitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,282,4210AG13.9% 127.1 / 8.4 80intergenic (+439/+122)narI/tprnitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base A (25/36);  new base G (8/5);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTC  >  CP009273/1282273‑1282566
                                                                                                                                                     |                                                                                                                                                  
tcctCCTTACACGCTGGGCCAGAAGATAAGATTGGGGCTGCGTTGCAGAATTCCGTGGTTTTTGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGa                                                                                                                                                    <  2:1042058/146‑1 (MQ=14)
    ccgtccacgtgtGGGCAGCAGGTTTAATTGGGTATTCGTTTGAGAATTCCGTGGTTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                                                <  2:208507/138‑1 (MQ=14)
      ttCCCCGCTTGGGCAGAATATTTGATTGGGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGCGTCGTTGCCTGCGGCAACGCTCTCTCGCTGAcgcgcg                                                                                                                                              >  1:808579/1‑149 (MQ=255)
      ctCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCGTTTGAAAGTGATGGTGGGGGGGGAAGGATCACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCg                                                                                                                                              >  2:924492/2‑149 (MQ=255)
       tCCCCGCTTGGGCAGAATATTTGACTGCGGATTCGTTTGAGAATTCCGGGGTTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCCGGAATGGGCCTGCGGCAACGCTCTCTCGCTGACGCTCGa                                                                                                                                             <  1:606676/149‑1 (MQ=14)
         cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAgt                                                                                                                                           <  2:498521/149‑2 (MQ=255)
          ccGCTTGGGCAGATTATTTGATTGCGGTGTCGTGTGAGAATTCCGGGGCTTGTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTACCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                                          <  1:35190/149‑1 (MQ=14)
            gCTGGGGCAGAATGTTTGATTGGGGATTCGTTGGAGAATGCCGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCTTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGa                                                                                                                                        <  1:1105523/149‑1 (MQ=14)
             cTTGGGCAGCATCGTTGATTGCGGATTCGTTTGAGAATTCCGGGGGGTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                       <  2:69394/149‑1 (MQ=14)
               tGGGCGAAATGTATGATTGGCGAGTGGTTTGAGGTTTTGGTGACTTGTGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAcc                                                                                                                                     <  2:578339/149‑1 (MQ=11)
               tGGGCAGAATATTTGATTGTGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAcc                                                                                                                                     >  1:1196467/1‑149 (MQ=255)
                   cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTGGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCttgg                                                                                                                                 >  2:1138464/1‑147 (MQ=255)
                    aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                                                >  1:977489/1‑149 (MQ=255)
                      aaTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGCAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCg                                                                                                                              >  1:722521/1‑149 (MQ=255)
                             gcttGCGGTTTCGTTGGAGGATTCCGGGGCTTGTGAAAGTGATGGTGGTGGGGGAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                       <  2:1052661/147‑1 (MQ=17)
                             gATTGCGGATTCGTTTCAGAGTTCCGGGGCTGTGGGAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTGCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                       <  1:529838/149‑1 (MQ=14)
                                   ggggtCGTTTGAGGATTCCGGGGCTTTTGGGAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                <  2:616728/145‑1 (MQ=255)
                                         gTTTGAGGATTCCGGGGCTTTTGGAAGGGATGGTGGTGGGGGAAGGAGTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                          <  1:598590/149‑1 (MQ=14)
                                         gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                          >  1:1015840/1‑149 (MQ=255)
                                         gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                          <  1:61802/149‑1 (MQ=255)
                                         gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGGCGAAGCTTCTCATCCTTcccc                                                                                                          >  1:933938/1‑149 (MQ=255)
                                         gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTCCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                          <  1:976431/149‑1 (MQ=255)
                                         gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTTCGGGTCGTTGCCTCCGGCAACGCCCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCTTCTCAGCCTTcccc                                                                                                          >  1:113819/1‑149 (MQ=14)
                                          tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCGTCAGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCGCATCCTGCCCCg                                                                                                         >  1:233494/1‑149 (MQ=255)
                                          tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCAGCTCGCTTGGGCGTTGGGGAGGTTACGTGCGGCACCGCTAGTGCGGTGCCGCGCGGGCCGCGCCTCAGTCGAAACTTCTCATCCTTCCCCg                                                                                                         >  2:200835/1‑149 (MQ=9)
                                              agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGGCAGGGATGGGGACCTTGCCCTTGAGGGCGTTGCCTGCAGCGACGCTCTCGCGGTGAGGCGCGAGCCGAAAATTAGACGAAGCTTCTCGTCCTTCCCCGCAtc                                                                                                     >  2:570640/1‑148 (MQ=9)
                                               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTgg                                                                                                    >  1:579646/1‑149 (MQ=255)
                                               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGGACCGTAGTCGAAGCTTCTCATCCTTCCCCGCGTgg                                                                                                    >  1:78039/1‑149 (MQ=14)
                                                gttctgggggGTTTGGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                   <  2:1073419/143‑1 (MQ=11)
                                                aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGGTCTCTCGCTGGCGCGCGGGCCGAACACTAGTCGAAGCCTCTGATCCTTCCCCGCAAggg                                                                                                   >  2:1176466/1‑149 (MQ=14)
                                                aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATAACTCAACGCCGCGCGCTACGGCCGTGGGTCAGCACCCGGCTACAACGCTCTGTCGGCCGCACCCTAGACGAACAATAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                   >  2:173822/1‑149 (MQ=2)
                                                          cTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGGGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                         <  2:248513/149‑1 (MQ=255)
                                                            tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAGCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGACAGACTAtttca                                                                                       >  2:1033147/1‑147 (MQ=255)
                                                                aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGc                                                                                   <  1:500707/149‑1 (MQ=255)
                                                                 aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCGTTCGGGTCGTTGCCTGCGGCACCGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAgtacggaattgcg                                                                                  >  2:5797/1‑139 (MQ=11)
                                                                       tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                                          <  2:448969/88‑1 (MQ=38)
                                                                          tggtggGGGAAGGATTCCTCAGCGCTGCGCTCTTCGTCTTTCGGTACGTCGCCTGCCGAGACGCTCTCTCGCTGTCGCACGCGCTCCCTACTCGTCGCTCTTCCGATCTCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                         <  2:808579/149‑1 (MQ=2)
                                                                          tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCTGAATATTTGATTGCGGATTCGCtt                                                                         <  1:924492/149‑1 (MQ=255)
                                                                               ggggAAGGCTTACTCCGCGGATTTCGTTTCTCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATGTGATTGCGGATTCGCTTGtgag                                                                    <  2:562576/149‑1 (MQ=14)
                                                                               ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                   >  1:1176315/1‑118 (MQ=40)
                                                                                     ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTGGCTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTGGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATGTTTGATTGCGGGTTCGGTTGATGGTTCAgg                                                              >  1:606033/1‑149 (MQ=14)
                                                                                     ggATTACTCAGCGCTGCGCACTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGATAATCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGCGTTCAgg                                                              >  1:902340/1‑149 (MQ=17)
                                                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAggg                                                             >  2:1238206/1‑149 (MQ=255)
                                                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAGTATTTGATTGGGGGTTCGCTTGGGGGTTCAggg                                                             >  2:936312/1‑149 (MQ=14)
                                                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATGTTTGATTGCGGATTCGCTGGGGAGTTCAggg                                                             >  2:636850/1‑149 (MQ=255)
                                                                                       cttACTCAGCGCTCCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTTCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                            <  1:887344/148‑1 (MQ=255)
                                                                                       aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                            <  1:899955/149‑1 (MQ=255)
                                                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                        <  2:921516/149‑1 (MQ=255)
                                                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                        <  1:1236931/149‑1 (MQ=255)
                                                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                        <  1:339131/149‑1 (MQ=255)
                                                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTGATTGCGGATTCGCTGGGGAGTTCAGGGGCTTTTGcaa                                                   >  2:451664/1‑149 (MQ=11)
                                                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGCTTCGCTTGAGACGTCAGGGACCTTTGaaa                                                   >  2:1147652/1‑149 (MQ=255)
                                                                                                cgcTGCGCGCTTCGCCCTTCGGGTCACTGCCTGCGGCAACGCTCTCTCGCGGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                   <  2:198868/149‑1 (MQ=255)
                                                                                                  cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                                 <  1:999482/149‑1 (MQ=255)
                                                                                                         gttCGCCCTTCGGGTCGGTGCCGGCGGTAACGCTCTCCCGCTGACGCCCGAGCCGAACCTTAGTCGAAGCTTCTCAGCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtg                                          <  2:180570/148‑1 (MQ=17)
                                                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCCCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtgg                                         <  1:29794/149‑1 (MQ=255)
                                                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCCCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtgg                                         <  1:1160131/149‑1 (MQ=255)
                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                >  2:502632/1‑75 (MQ=25)
                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                <  1:502632/75‑1 (MQ=255)
                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                      <  2:664291/149‑1 (MQ=255)
                                                                                                               cgttCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                    <  2:1103084/147‑1 (MQ=255)
                                                                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                    <  2:1102951/149‑1 (MQ=255)
                                                                                                                    gggTCCTAGCCTGCCGCAACGCCCTCCCCCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATAGCCAGAATATTGGAATCCGGATTCGCTTGAGAATTCAGAGACCTTCGAAAGCGATGGCGGTGGCGGAAgg                               <  2:664794/149‑1 (MQ=14)
                                                                                                                     ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                              <  2:98681/149‑1 (MQ=255)
                                                                                                                      gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGAAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                             <  1:570640/149‑1 (MQ=255)
                                                                                                                       tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGGTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAGGTGATGGTGGTGGGGGAAGGGtt                            >  1:401963/1‑149 (MQ=255)
                                                                                                                       tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGGTGGTGGTGGGGGGAGGAtt                            >  1:274422/1‑149 (MQ=255)
                                                                                                                        cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGTACCTTGGTCGGAGCTTCTCCTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATGCGGTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                           <  2:1026302/149‑1 (MQ=11)
                                                                                                                        cGTTGCCGCCGGCAACGCTCCCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTGATTGTGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                           <  2:172433/149‑1 (MQ=11)
                                                                                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACTCTCGCCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAAGTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                     >  2:323035/1‑149 (MQ=14)
                                                                                                                                tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTaggccgct                    >  2:888205/1‑142 (MQ=255)
                                                                                                                                tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTGCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACCCAgcg                    <  1:200835/149‑1 (MQ=255)
                                                                                                                                   ggCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg                 <  2:1043267/149‑1 (MQ=255)
                                                                                                                                          ctgtctcGCTGCCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTCCCCCGCATGGGCAGAATATTTGATTGCGGATCCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt          <  1:564462/146‑1 (MQ=14)
                                                                                                                                          ctctctCGCTGTCGCTCGAGCCGGACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGGTTGCGGATACGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt          <  2:387451/149‑1 (MQ=255)
                                                                                                                                          ctctctCGCTGACTATCGAGCCGACGCTAATTTTTATCCACTCGACCTTCCACGCTCGGGCCCTACATGTGGCTGCGGAGTCGTTTGAGGTTTCAGGGACTTTTGGAAGTGATGGTGGTGGGGGAAGGTATACTCAGCGCTgcgagatg          <  2:566063/149‑6 (MQ=2)
                                                                                                                                           tctctcGCTGACTCGCGAGCCGAACCTTAGTCGAACCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc         <  1:691012/149‑1 (MQ=255)
                                                                                                                                             tctcGCTGCCGCTCCAGCCGACCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc       <  1:684506/149‑1 (MQ=255)
                                                                                                                                             tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                                                >  2:1061246/1‑60 (MQ=255)
                                                                                                                                             tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGATTAGTCCGCGCTacgcgcctggc       >  2:363441/1‑144 (MQ=17)
                                                                                                                                                  cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACATCGGAAAGGGGTGGTGGAGGGGAAAGGAGTACTCAGCGCTGCGCGCCTCGCCGTTc  >  2:873011/1‑149 (MQ=17)
                                                                                                                                                     |                                                                                                                                                  
TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTC  >  CP009273/1282273‑1282566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: