Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,384,896 C→G 4.2% W30S (TGG→TCG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,384,8960CG 4.2% 222.1 / 2.3 81W30S (TGG→TCG) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (31/46);  new base G (2/2);  total (33/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

CCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGCCAA  >  CP009273/2384751‑2385043
                                                                                                                                                 |                                                                                                                                                   
cccGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCTCAATAACCGTGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACg                                                                                                                                                  <  2:649138/149‑1 (MQ=255)
cccGGTGCAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACg                                                                                                                                                  <  1:215612/149‑1 (MQ=255)
 ccGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGc                                                                                                                                                 >  2:72230/1‑149 (MQ=255)
   ggggtAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAATCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCaa                                                                                                                                               <  2:1187867/146‑1 (MQ=255)
    gtgtAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAAt                                                                                                                                              <  2:747231/149‑1 (MQ=255)
    gtgtAAAGCATGGCGAAACCACCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCGGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGCAATGATTGCGTCGCCACGCAAt                                                                                                                                              <  1:491003/149‑1 (MQ=255)
    ggggaaaGCAGGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAAt                                                                                                                                              <  2:651947/145‑1 (MQ=255)
       tAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCACGGTAAGCCCAAAAACCGAGAGCGTAGCGTTGAGGAAATAATGGCGTCGCCAAGCAATGga                                                                                                                                           >  1:104101/1‑149 (MQ=255)
        aaaGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGGGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                                                                          <  1:1023813/149‑1 (MQ=255)
           gCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                                                                       <  1:62366/149‑8 (MQ=255)
            cATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGCAGCGAGGAGAACACGATGGCGTCGCGACGCAAAGGggagaa                                                                                                                                      >  2:712172/1‑147 (MQ=255)
             aTGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCAt                                                                                                                                     >  1:761538/1‑149 (MQ=255)
             aTGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCAt                                                                                                                                     <  2:130212/149‑1 (MQ=255)
              tGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACGAGAGCGAAACCTGCGCCGCGTTAAGCCCAATAACCGAGAGCGTGGCTATGTAATAAGTCTGGCGTCGCCACGCAATGGAGAGCATc                                                                                                                                    <  2:575912/149‑1 (MQ=255)
              tGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATc                                                                                                                                    <  1:899201/149‑1 (MQ=255)
               ggcgaaccccgtaaCCCGCAGCAGCCGCGTAACGGCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATACCCGACAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                                                                   <  1:175505/137‑1 (MQ=255)
               ggCGAAACCATCACCGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                                                                   <  1:10975/149‑1 (MQ=255)
                   aaaCCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAac                                                                                                                               >  1:315762/1‑149 (MQ=255)
                         tCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGAGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacaaccac                                                                                                                         >  1:109697/1‑149 (MQ=255)
                          cAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccacc                                                                                                                        <  1:562086/149‑1 (MQ=255)
                           aaCGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                                                                       <  2:92395/149‑1 (MQ=255)
                                 cATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                                                                 <  1:975706/149‑1 (MQ=255)
                                     aGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                                                                             <  1:1016705/149‑1 (MQ=255)
                                       cggcgTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGGAGCGTTGAGGAAATGATTGCGTCGCCACGCAAGGGAGCGCGTCACAACCACCACCGGCAAGCcgacga                                                                                                           >  2:127011/1‑149 (MQ=255)
                                        ggcgTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGAAAAGCACCACAACCACCACCGTCAAGcccccgaa                                                                                                          >  1:108781/1‑143 (MQ=255)
                                             aaCGCCCCTAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATACCCGAGAGCGTATCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCCAGCCGACGATCAgga                                                                                                     <  2:213515/149‑3 (MQ=255)
                                              aCGTCCATATCGCCCGCCTGGCCAACAAACCAGAGCGAAACAAGTGTTATGTCGAGCTTAGTAAGCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAACCCGACGATCCGCaa                                                                                                    >  1:293871/1‑149 (MQ=255)
                                               cGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATAAGCAAc                                                                                                   >  2:1028982/1‑149 (MQ=255)
                                                gTCCAGAGCGCCCGCCCGGCCAACAAACCAGATCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCCACGATCAGCAACg                                                                                                  <  1:845941/149‑1 (MQ=255)
                                                 tCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGCCGATCAGCAAcag                                                                                                 >  1:279734/1‑147 (MQ=255)
                                                            cgcaTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                                                                      <  2:611362/149‑1 (MQ=255)
                                                             gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGACATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGCCGAGCAGCAACGGTAGCAGTGCGag                                                                                     >  2:746993/1‑148 (MQ=255)
                                                             gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACACCCGCCGCCTTCAAGCCGACGATCAGCAACGGTAGCAGTGCGAt                                                                                     >  1:648231/1‑149 (MQ=255)
                                                                tGGCCAACACACCATAGCGAAACCAGCGCCGCGTTAAGCCCCATCACCGAGAGCGTAGCGTTGAGGAACGGCTGGCGTCGACACACAATGGAGATCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAg                                                                                  <  1:600656/149‑1 (MQ=255)
                                                                tGGCCAACAAACCAGGGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAg                                                                                  <  2:185479/149‑1 (MQ=255)
                                                                tGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAg                                                                                  <  2:315762/149‑1 (MQ=255)
                                                                  gCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                                                                                >  1:195435/1‑149 (MQ=255)
                                                                  gCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                                                                                >  1:513401/1‑149 (MQ=255)
                                                                    caacaaACCAGAGCGAAACCAGCGCTGCGTGAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGttt                                                                              >  1:639942/1‑149 (MQ=255)
                                                                       caaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgt                                                                           <  2:353378/149‑1 (MQ=255)
                                                                       caaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAAAGATTGCGCCGCCACGCAATGGAGAACATCAAAACAACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGCACGAACAgatatagt                                                                           >  2:165273/1‑142 (MQ=255)
                                                                        aaaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGGTTtgtg                                                                          >  1:934953/1‑149 (MQ=255)
                                                                         aaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTAAGACCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTgg                                                                         >  1:841908/1‑149 (MQ=255)
                                                                           ccAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGaa                                                                       >  1:949296/1‑148 (MQ=255)
                                                                           ccAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAg                                                                       <  1:188183/149‑1 (MQ=255)
                                                                               agCGAAACAAGCGCCGCGTTAAGCTCAATAACCGGTAGCGTAGCGTTGAGGAAATGTGTGCGTCGCCACGCAATGGAGAGCAGCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCACGTTTTGTGGAGTTAt                                                                   <  2:689629/149‑1 (MQ=255)
                                                                                gCGAAACAAGCGCCGCGTTAAGCGCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTGGTGGAGTTAtt                                                                  >  2:842499/1‑149 (MQ=255)
                                                                                ccGAAACCAGCGCCGCGTTAAGCCCAATGACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                                                                  <  2:387319/148‑1 (MQ=255)
                                                                                 cGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCGGTGCGATCAGGTTTTGGGGAGTGATTg                                                                 >  1:272514/1‑149 (MQ=255)
                                                                                  gAAACCAGCGCCGCGTCAAGCCCAATAACCGAGAGCGTAGCGTGGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCCCCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTGATTGt                                                                <  2:23357/149‑1 (MQ=255)
                                                                                    aaCCTGCGCCGCGTTAAGCCAACAAAGCGAGAGCGTAGCGTGGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCATCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa                                                              <  1:524158/149‑1 (MQ=255)
                                                                                    aaCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa                                                              <  1:108667/149‑1 (MQ=255)
                                                                                    aaCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGAAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCCCCACCGTCAAGCGGACGAGCAGCACCGGTAGCCGGGCGCGCAGGGTTTGTGGCGTTAGTGTcc                                                              >  2:431272/1‑148 (MQ=255)
                                                                                      ccatcgcgccgTCAACCCCAACAGCCGAGAGGGTAGCGTTGAGGAAATGATTGCGGCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg                                                            <  2:980785/140‑1 (MQ=255)
                                                                                      ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGACCAGGCTTTGTGGAGGTCTTGCCATg                                                            >  2:1216781/1‑149 (MQ=255)
                                                                                      ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAAGGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCCGTGCGATCAGGGTTTGTGGAGTAGTTGTCAtc                                                            >  2:818976/1‑148 (MQ=255)
                                                                                       cAGCGCCGCGTTAAGCCCAATAACCGAGGGCGTAGCGTTGAGGAAATCATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGGAGTTGTGCGATCAGGTTTTGTGGAGTTATTGTCATgg                                                           <  2:1190699/149‑1 (MQ=255)
                                                                                       cAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATgg                                                           <  2:233903/149‑1 (MQ=255)
                                                                                             cgcgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCCCGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAttt                                                     >  1:965115/1‑149 (MQ=255)
                                                                                                 ttAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACgg                                                 <  1:72230/149‑1 (MQ=255)
                                                                                                 ttAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACgg                                                 <  1:748297/149‑1 (MQ=255)
                                                                                                        cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgta                                          <  1:1136693/149‑1 (MQ=255)
                                                                                                        cAATAACCGAGAGCGGAGGGTTGATGAAATGATTGCGTCGGCACGTAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgta                                          <  2:788257/149‑1 (MQ=255)
                                                                                                         aaTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtag                                         >  1:512118/1‑149 (MQ=255)
                                                                                                             aCCGAGAGATAAGCGTTGGGGAAATGATTGCGCAGCCACGCAATGGAGAGCATCACAGCCACCACCGTCAAGCCGACGATCGGCAACGGTAGCAGTGCGATCAGGGTTAGTGGAGTTATGGTCATGGCGATTTACGGGCtgatagaagc                                     >  2:1074201/1‑140 (MQ=255)
                                                                                                              ccggcagcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagtagta                                    <  2:26851/144‑1 (MQ=255)
                                                                                                              ccGAGCGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagtagta                                    <  2:1008692/149‑1 (MQ=255)
                                                                                                              ccGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGGATCACAACCACCACCGGCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagtagta                                    >  2:609467/1‑149 (MQ=255)
                                                                                                               cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTCGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTaa                                   >  1:653504/1‑149 (MQ=255)
                                                                                                               cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTaa                                   >  2:929226/1‑149 (MQ=255)
                                                                                                                 agagCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACg                                 <  1:1201687/149‑1 (MQ=255)
                                                                                                                 agagCGTAGCGTTGAGGAAACGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACg                                 <  1:1010431/149‑1 (MQ=255)
                                                                                                                 agagCGTAGCGTGGAGGAAATGATTGCGTCGCCACGCAACGGAGAGCAGCACAACCACCACCGTCAAGCCGACGATCAGCAACGGGAGCAGTGCGATCAGGTTTTGTGGTGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACg                                 <  1:357804/149‑1 (MQ=255)
                                                                                                                    gcgtagcgtTGAGGAAGTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCCCCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGaa                              <  1:448945/149‑1 (MQ=255)
                                                                                                                            ttGAGGAAAGTATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACaaa                      <  2:742209/149‑1 (MQ=255)
                                                                                                                               tgctctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCa                   >  2:357229/9‑149 (MQ=255)
                                                                                                                                    atgatgGCTTCGACACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCActgct              <  1:222922/149‑1 (MQ=255)
                                                                                                                                    aTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCActgct              <  2:1207082/149‑1 (MQ=255)
                                                                                                                                         tGCGTCGCCACGCAATCGCGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGata         <  1:1084070/149‑1 (MQ=255)
                                                                                                                                            gTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTg      >  2:1123665/1‑149 (MQ=255)
                                                                                                                                             gcGCCCCGTAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGc     <  1:467374/148‑1 (MQ=255)
                                                                                                                                                ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGGCaa  >  1:181290/1‑149 (MQ=255)
                                                                                                                                                ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGCCaa  >  1:527945/1‑149 (MQ=255)
                                                                                                                                                ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGAAATTGcaag  >  1:781568/1‑146 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGCCAA  >  CP009273/2384751‑2385043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑