Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,824 C→T 31.1% E214E (GAG→GAA recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,8240CT31.1% 99.6 / 53.4 81E214E (GAG→GAArecJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base C (26/29);  new base T (9/16);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCG  >  CP009273/3030676‑3030971
                                                                                                                                                    |                                                                                                                                                   
tCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGc                                                                                                                                                     <  1:624489/149‑1 (MQ=255)
cccccGGACGGCACCTCCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGc                                                                                                                                                     <  1:121706/148‑1 (MQ=255)
  cccGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGACTGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTTCGATGTTACGCta                                                                                                                                                   >  2:239272/1‑148 (MQ=255)
  cccGGACGGCACTTTCCGGCTCGAACGCGAGTCACCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCTCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCtc                                                                                                                                                   <  2:808538/149‑1 (MQ=255)
       aCGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCaa                                                                                                                                              <  2:221630/149‑1 (MQ=255)
       aCGGCACTTTCCGGCTCGGATGCGACCCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCaa                                                                                                                                              <  1:478560/149‑1 (MQ=255)
           cACTTCCCGGCTCGGAGGCGACCCACCCCCTGCCAGGTCAGACTGCGATTTTTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCa                                                                                                                                          <  2:983792/149‑1 (MQ=255)
            gccttCCGGCTCGGAGGCGACTCATCCCCTGCCAGGCCAGAATGCGATTATTAGCGTCCAGCGGCACGACGGCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAg                                                                                                                                         <  1:976401/146‑1 (MQ=255)
             cTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGc                                                                                                                                        <  1:576830/149‑1 (MQ=255)
                      tCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGCTcgc                                                                                                                               >  1:198331/1‑149 (MQ=255)
                       cGGATGCGACTCGTCCCCTGCCCGGTCAGAATGCGTTTACTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATcgcg                                                                                                                              <  2:870187/149‑1 (MQ=255)
                       cGGATGCGACTCATCTCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGGCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATcgcg                                                                                                                              <  1:870014/149‑1 (MQ=255)
                           tGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCaaa                                                                                                                          >  1:526118/1‑149 (MQ=255)
                            gCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCaaaa                                                                                                                         >  1:457122/1‑149 (MQ=255)
                             cGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGAAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCaaaaa                                                                                                                        >  1:836565/1‑149 (MQ=255)
                              gACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAg                                                                                                                       <  1:1228790/149‑1 (MQ=255)
                              caCTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAg                                                                                                                       <  1:671781/148‑1 (MQ=255)
                                cTCATCCCCTGCCAGGTCAGACTGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGt                                                                                                                     <  1:948141/149‑1 (MQ=255)
                                  cATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCGAGCGGCACGAAGTCGGCGGCTGTCCCGAGCGCGACCAGATCGAGCGGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCGGACCTGATCGCGCACAAAGGTgc                                                                                                                   >  2:606017/1‑149 (MQ=255)
                                  cATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTGCGTTCATCTAACCATCCCTGATCGCGCAAAAAGGTgg                                                                                                                   >  2:199141/1‑148 (MQ=255)
                                  cATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGGTCATCAAACCAGCCCTGATCGCGCCAAAAGCTgc                                                                                                                   >  2:390156/1‑149 (MQ=255)
                                   agccccTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTgcg                                                                                                                  <  1:1062909/147‑1 (MQ=255)
                                   aTCCCCTGCCAGGTCAGAATGCGATTCTTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTgcg                                                                                                                  <  1:1064096/149‑1 (MQ=255)
                                   aTCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTgcg                                                                                                                  <  1:412466/149‑1 (MQ=255)
                                        cTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAAGGGCCCAgcg                                                                                                             >  1:1016747/1‑149 (MQ=255)
                                         tGCCAGGTCAGCGTGGGATTATTCGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAgcgc                                                                                                            <  2:999927/149‑1 (MQ=255)
                                           ccAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGCTGTTACGCTCATCAAACCAGCCCTGAGAGCGCAAAAAGGCGCGGCGCGccc                                                                                                          >  2:1085421/1‑148 (MQ=255)
                                           ccAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCa                                                                                                          <  2:605345/149‑1 (MQ=255)
                                           ccAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCa                                                                                                          >  2:337602/1‑149 (MQ=255)
                                            cAGGTCAGAATGCGATTCTTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGATCAACAAGGGTACTCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAg                                                                                                         <  1:853644/149‑1 (MQ=255)
                                            cAGGTCAGAATGCGATTATCAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAg                                                                                                         <  1:335910/149‑1 (MQ=255)
                                             aGGTCAGAATGCGCTTATTAGTGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATACAGCAGTCCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGc                                                                                                        <  2:328909/149‑1 (MQ=255)
                                               gTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACGAGCCCTGATCCGGCAAAAAGGTGCGCGGCGCCAGCAt                                                                                                      >  2:767985/1‑149 (MQ=255)
                                                 cAGAATGCGATTATTAGCTTCCCGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCa                                                                                                    <  2:178660/149‑1 (MQ=255)
                                                    aaTGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGAt                                                                                                 <  1:839667/149‑1 (MQ=255)
                                                       gCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATaaa                                                                                              <  1:441090/149‑1 (MQ=255)
                                                       gCGATTATTACCGCCCAGCGGCACGACGTCCCCCACTGTCCCCAGTGCAACCAGATCCAGGAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCTTTGATTGCGCAATAATGTGCGCAGCGCCAGCATCAGATaaa                                                                                              <  2:358324/149‑1 (MQ=255)
                                                        cGATTATTAGCGTCCAGCGGCCCGACGTCCGCCACTGTCCCCAGCGCGACCAGCTCCCGCCGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCTCAAAAAGGTGCGCAGCGCCAGCATCAGATaaaa                                                                                             <  1:422779/149‑1 (MQ=255)
                                                        cGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATaaaa                                                                                             <  2:825968/149‑1 (MQ=255)
                                                          attatgAGCGTCCAGCGGCACGACGTCCGCGACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGCTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCAAAAAACAAAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACg                                                                                           <  2:94883/149‑1 (MQ=255)
                                                            tattaGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCCGTTCTGCCAGGTTAGGAATTGCGATGTTACACTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGAAGCGCAAGCATCACATGAAACGcc                                                                                         >  2:1020982/1‑149 (MQ=255)
                                                            tattaGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGcc                                                                                         >  2:447780/1‑149 (MQ=255)
                                                             attaGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCca                                                                                        <  1:782137/149‑1 (MQ=255)
                                                                  cGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAAGGCCACAccc                                                                                   >  2:296946/1‑149 (MQ=255)
                                                                   gTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCa                                                                                  >  1:502938/1‑149 (MQ=255)
                                                                    tCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATAACGATGTTACGCTCATCAAACCAGCCCTGATCGCGAAAAACAGTGAGCAGCGCCAGCATCCGATACAACGCCACAccccc                                                                                 >  2:34643/1‑147 (MQ=255)
                                                                    tCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTATGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATAGCGCAAAAAGGGCCGCAGCGCCAGCATCCGATAAAACGCCACACCAAc                                                                                 >  2:706430/1‑149 (MQ=255)
                                                                     ccAGCGGCACGACGTCCGCCACTGCCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACg                                                                                <  1:713408/149‑1 (MQ=255)
                                                                      cAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCGGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGc                                                                               <  2:640022/149‑1 (MQ=255)
                                                                      cAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGc                                                                               <  2:300764/149‑1 (MQ=255)
                                                                      cAGCCGCACGACGTCCCCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGc                                                                               <  2:355900/149‑1 (MQ=255)
                                                                              cgacgTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCCGATACAACGCCACACCCACGCCTGCCAga                                                                       >  1:381179/1‑148 (MQ=255)
                                                                                   tCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGAttt                                                                  <  1:823897/149‑1 (MQ=255)
                                                                                      gCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGa                                                               >  1:108872/1‑149 (MQ=255)
                                                                                      gCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAGCGCCACACCCACGCCTGCCAGTGATTTCGa                                                               >  1:279314/1‑149 (MQ=255)
                                                                                          cTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCTACGCCTGCCAGTGATTTCGACggg                                                           <  1:920536/149‑1 (MQ=255)
                                                                                           tGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTCACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGa                                                          >  2:390832/1‑149 (MQ=255)
                                                                                              ccccAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAAt                                                       <  2:507288/149‑1 (MQ=255)
                                                                                                ccAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGGCACGGATTTCGACGGCAAATTa                                                     >  1:271369/1‑149 (MQ=255)
                                                                                                 cAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTAc                                                    <  1:1007597/149‑1 (MQ=255)
                                                                                                 cAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACACTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTAc                                                    <  1:471135/149‑1 (MQ=255)
                                                                                                  atcgcgACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGAGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACa                                                   <  1:161714/147‑1 (MQ=255)
                                                                                                     gcgACCAGATCCAGCAGTTCTGCCAGGTTTGGGATTGTGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTc                                                <  1:955758/149‑1 (MQ=255)
                                                                                                     gcgACCAGATCCAGCAGTTCCGCCATGTTAGGAATTGCGATGCTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCCCTCCTGCCAGTGATTTCGACGGGAAATTACAGTc                                                <  2:1007208/149‑1 (MQ=255)
                                                                                                       gACCAGAGCCAGCAGTTCGGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTcgc                                              <  1:3274/149‑1 (MQ=255)
                                                                                                           aGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGCTCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAGTTACAGTCGCGCaa                                          >  1:105646/1‑149 (MQ=255)
                                                                                                           aGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCaa                                          >  1:1217289/1‑149 (MQ=255)
                                                                                                            gATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAg                                         >  2:115854/1‑149 (MQ=255)
                                                                                                             aTCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGt                                        >  1:396077/1‑149 (MQ=255)
                                                                                                                     gTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTa                                >  1:338284/1‑149 (MQ=255)
                                                                                                                      ttCTGCCAGGTTAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTAGACGAGCAAATACAGgcgcgctcggcttctaacg                               >  1:257477/1‑136 (MQ=255)
                                                                                                                      tgcagCCAGGTTAAGAATTGCGATGTTACGTCCAACAAACCAGCCCTCATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTaa                               <  1:220625/145‑1 (MQ=255)
                                                                                                                                ttAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCACGTTAGGGTTAATGATTGCtca                     >  2:1112666/1‑147 (MQ=255)
                                                                                                                                ttAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTc                     >  2:1236417/1‑149 (MQ=255)
                                                                                                                                 tAGGAATTGCGATGTTACGTTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTcc                    >  1:252538/1‑149 (MQ=255)
                                                                                                                                   ggAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGc                  >  1:959533/1‑149 (MQ=255)
                                                                                                                                   ggAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATATCGACGGGAAATTACAGTCGCGCAAGTTAAGGTTAATGATCGCTTCCGc                  >  1:520636/1‑149 (MQ=255)
                                                                                                                                        tGCTATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCgg             <  1:233390/149‑1 (MQ=255)
                                                                                                                                        tGCGATGTTCCGCTCATCAAACCCGCCCGGATCGCGCAAAAAGGCGCGGAGCGCCAGCATCAGATAAAACGCCACCGCCACGCCTGCGAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCgg             <  2:346637/149‑1 (MQ=255)
                                                                                                                                        tGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCgg             <  2:995683/149‑1 (MQ=255)
                                                                                                                                        tGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTACATCCGCCGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCgg             <  1:659350/149‑1 (MQ=255)
                                                                                                                                             tGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCACAGCACAACCATCAGATAAGACGCCACCCCCACGCAAGCCAGTTATTTCGACGGGCACTTACACTAGCGCGAGGTAGGGCTAGTGAACGCTTCCGCTGAGGGCAAt        >  2:345934/1‑149 (MQ=255)
                                                                                                                                                   gCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCg  >  1:888058/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCG  >  CP009273/3030676‑3030971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: