Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,529,197 A→G 69.8% S11P (TCA→CCA)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,529,1970AG69.8% 114.4 / 49.4 88S11P (TCA→CCA) envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base A (13/14);  new base G (29/32);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATG  >  CP009273/3529053‑3529344
                                                                                                                                                |                                                                                                                                                    
tcatcaACATACGCACTTCGTACTGGAGGTCTTTATTTATCTGCTGGAGTCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt                                                                                                                                                  <  1:537855/149‑1 (MQ=255)
tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt                                                                                                                                                  <  1:676195/149‑1 (MQ=255)
tcatcaACATACGCACTTCGTACGCGAAGACTTTATTTCTCTGTTTGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt                                                                                                                                                  <  1:537915/149‑1 (MQ=255)
  atcaACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCg                                                                                                                                                <  1:517990/149‑1 (MQ=255)
    caACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTg                                                                                                                                              <  1:951705/149‑1 (MQ=255)
     aaCATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTgg                                                                                                                                             <  2:835525/149‑1 (MQ=255)
     aaCATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTgg                                                                                                                                             <  2:1094576/149‑1 (MQ=255)
     aaCATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTgg                                                                                                                                             <  1:511826/149‑1 (MQ=255)
     aaCATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGCCCGGCCAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTgg                                                                                                                                             <  1:243304/149‑1 (MQ=255)
     aaCATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCGCGGCAAAATCGCGAAGTTCATCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTgg                                                                                                                                             <  2:9386/149‑1 (MQ=255)
       cATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCg                                                                                                                                           >  2:613413/1‑149 (MQ=255)
        aTACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTCCGGGCCAATGGCCTTCGTGGCgc                                                                                                                                          >  1:93293/1‑148 (MQ=255)
        aTACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGACAAATGAACTTCGTGGCga                                                                                                                                          >  1:1040135/1‑149 (MQ=255)
         tACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACAGGCAAATGAACTTCGTgggcac                                                                                                                                         >  2:1125879/1‑145 (MQ=255)
         tACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAATTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATACCGTACGGGCAGATGAACTTCGTGGCgag                                                                                                                                         >  2:636952/1‑149 (MQ=255)
           cccACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGaa                                                                                                                                       <  2:159640/147‑1 (MQ=255)
           cccACTGCGTACGCGAGGACTTTATTTAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGCGCAATAACGGACGGGCAACTGGGCTTCGTGGCGGGaa                                                                                                                                       <  2:423057/147‑1 (MQ=255)
           cGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGaa                                                                                                                                       <  2:159674/149‑1 (MQ=255)
           cGCACCTCGTACGCGAGGACTTGAGTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGaa                                                                                                                                       <  2:503356/149‑1 (MQ=255)
             ctcTTCGTACGCGAGGACTCTATTAAACTGCTGGAGGCTCGGCAAACTGTCGACGTTCCGCACCACCAGATAAGTCGTCACCAGGCGGGCGAACAGCAAGGTGACGAGGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAgc                                                                                                                                     <  2:542795/147‑1 (MQ=255)
             cACTTCGTACGCGATGACTTTCACAAACTGCGGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAgc                                                                                                                                     <  2:1238899/149‑1 (MQ=255)
                         gAGGCCTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCa                                                                                                                         >  2:632747/1‑149 (MQ=255)
                               tttATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCttt                                                                                                                   <  2:484805/149‑1 (MQ=255)
                                ttattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTa                                                                                                                  <  2:243029/149‑1 (MQ=255)
                                ttattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGACGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTa                                                                                                                  <  2:297108/149‑1 (MQ=255)
                                 tattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTag                                                                                                                 >  1:395028/1‑149 (MQ=255)
                                  atgaaaCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTaga                                                                                                                <  1:824349/146‑1 (MQ=255)
                                   ttaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTagag                                                                                                               >  1:373244/1‑149 (MQ=255)
                                    taAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGc                                                                                                              >  1:1103503/1‑149 (MQ=255)
                                     aaaCTGTGGGGGGCGCGGCAAAATCGCGAAGTTCAGCATCACCAGACAAGTCGTCACCAGGCTGGCTAACAGCAAGTTTTCGAGTAGCAATACCGTACGGGCAAAGGAACTTCGTGTTGAGAAGCGCAATCGCCTCATGCTTTAGAGcc                                                                                                            <  2:544262/149‑1 (MQ=255)
                                        ctgctgGAGGCTGGGCAAAATCGCGAAGTTCAGCACCAGCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAAGCGCCTCATGCTTTAGAGCCGTc                                                                                                         <  2:824236/149‑1 (MQ=255)
                                          gctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCg                                                                                                       <  1:175658/149‑1 (MQ=255)
                                           ctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCgg                                                                                                      >  1:718741/1‑149 (MQ=255)
                                           ctgGAGGCTCGGCAAAATCGCGAAGTTCACCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAAGGGACTTCGTGGCGAGAAGCGCAATCGCCCCATGCGTTAGAGCCGTCCgg                                                                                                      >  1:778678/1‑149 (MQ=255)
                                                 gCTCGGCAAAATCGCGAAGTGCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAACGGGCAAAAGAGCAATTACGGAGGGGCAAATGGACTTCATGGCGAGACGGGCCACGGCCCGCTGCATTAGGACCGCCCGGTACaca                                                                                                >  2:441332/1‑147 (MQ=255)
                                                   tCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGa                                                                                              <  2:303439/149‑1 (MQ=255)
                                                   tCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGa                                                                                              >  1:572296/1‑149 (MQ=255)
                                                   tCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAAAGCCGTCCGGTACCAAGa                                                                                              >  1:272185/1‑149 (MQ=255)
                                                    cGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCACTAACGTACGGGCAAATGGACTTCGGGGCGAGCAGCGCAGTCGCCCCATGCTTTAGAGCCGTCCGGGACAAAGAc                                                                                             >  1:31226/1‑149 (MQ=255)
                                                     ggCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAGGCGCAATCGCCTCATGGTTTGACGCCGTCAGGTACAAAGACg                                                                                            >  2:652310/1‑149 (MQ=255)
                                                     gccataccatcgAGGTTCAGCACCACCCGAAAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGGGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGGACAAAGACg                                                                                            >  2:1135909/11‑149 (MQ=255)
                                                      gCAAAATCGCGAAGTTCAGCACCACTAGGTAAGTCGTCACCAGGCTGTCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTAGTGGCGAGAAGCGCAATTGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGt                                                                                           >  1:697314/1‑149 (MQ=255)
                                                      gCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGGCCGCGGTCGGTGAGCAATAACGGACGGGCAAATGGACCTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGt                                                                                           <  1:903637/149‑1 (MQ=255)
                                                      gCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCCCGCTTTCGAGCCGTCCGGTACCAAGACGt                                                                                           >  2:870694/1‑149 (MQ=255)
                                                       cAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTa                                                                                          >  1:147950/1‑149 (MQ=255)
                                                            tCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGAGAACAGACAGGTGACGATGAGCAATAAAATACGGGCAAAGGGACTTCGTGGCGAGAAGCGCAATCGCCTCCTGCTTTAAGCACGTCCGGGCAAAAGACGTaacaca                                                                                     >  2:1019711/1‑144 (MQ=255)
                                                               cgAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagac                                                                                  >  1:131725/1‑149 (MQ=255)
                                                               cgAAGTTCAGCACCACCAAATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagac                                                                                  >  1:910513/1‑149 (MQ=255)
                                                                    ttCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccag                                                                             >  2:264638/1‑149 (MQ=255)
                                                                          accaccAGATAAGGCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAACAGCGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGACCAGACCCCAGACGGTc                                                                       <  2:916111/149‑1 (MQ=255)
                                                                            caccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAACAGCGTACGGGCAAAAGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAAAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGGCTg                                                                     >  2:930479/1‑149 (MQ=255)
                                                                             agcaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGAGGGGCAATACCGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTGAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGa                                                                    <  2:60609/147‑1 (MQ=255)
                                                                             accaGGTAAGTCGGCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGa                                                                    <  2:384713/149‑1 (MQ=255)
                                                                             accaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGa                                                                    >  1:254374/1‑149 (MQ=255)
                                                                               caGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCAAGACCCCAGACGGGCTGAAt                                                                  >  1:790324/1‑149 (MQ=255)
                                                                                      gtcgtcACCAGGCTGTCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAACCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAAcgc                                                           >  2:781443/1‑149 (MQ=255)
                                                                                      gtcggcAACAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGGACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAAcgc                                                           <  1:479181/149‑1 (MQ=255)
                                                                                       tcgtcACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAAcgcg                                                          >  2:487091/1‑149 (MQ=255)
                                                                                               aGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCCTGGCGAGAAGCGCAATCGACTCATGCTTTAGAGCCGTCCGGTACAAAGACCTAGCCCAGACCCTAGACGGTCTGAATGTAACGCGGAAGCGCt                                                  >  2:792395/1‑149 (MQ=255)
                                                                                                ggCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGACAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTg                                                 <  2:1040135/149‑1 (MQ=255)
                                                                                                 gCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTgg                                                <  1:701310/149‑1 (MQ=255)
                                                                                                   tGGCGAACAGCAAGGGGACGAGGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAt                                              <  2:85576/149‑1 (MQ=255)
                                                                                                       gAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATTGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAtcttc                                          <  1:356639/149‑1 (MQ=255)
                                                                                                       gAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTAGCGTTTAGCGCAATCGCCTCATGCGTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAtcttc                                          <  1:544274/149‑1 (MQ=255)
                                                                                                       gAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAtcttc                                          >  2:1139854/1‑149 (MQ=255)
                                                                                                            gCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTGGTGGCGAGAAGCGCAATGGCCTCATGCTTTAGAGCCGTCCGGGACAAAGACGTAGCCCAGCCCCCAGACGGGCCGAATGTAACGCGGCAGCGCTGGATCTGCCTcca                                     >  2:381507/1‑149 (MQ=255)
                                                                                                            gCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCACTCGCCTCATGCATTAGAACCGTACGGTACAAAGACGTAGCCCAGCCCCCAGACGGGCTTAATGTCACGCGGAAGCGCTGGATCTTCTTcca                                     >  2:155138/1‑149 (MQ=255)
                                                                                                             cAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTccac                                    <  2:334272/149‑1 (MQ=255)
                                                                                                             cAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTccac                                    <  2:333966/149‑1 (MQ=255)
                                                                                                                       aTGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGgcgc                          <  2:965072/149‑1 (MQ=255)
                                                                                                                          ggCAATCACGTCCGGGCAAAGGGACTTCGCGGCGGGAAGCGCCATCGCCCTACTCTTTAGAGCCGCGCGGTACAACGACGTAGCCCAGGCCCCAGACGGTCTGAATGTAACGCGGATGCGCTAGATCGTCACACACCGTGCGGCGCAGa                       <  2:585633/148‑1 (MQ=255)
                                                                                                                           ggaaTAACGTACGGGCAAATGAATTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAc                      <  2:465159/147‑1 (MQ=255)
                                                                                                                           gCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAc                      <  2:826066/149‑1 (MQ=255)
                                                                                                                           gCAATAACGTACGGGCAAATGGACTCCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACTTAGCCCCGTCCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAc                      <  2:992715/149‑1 (MQ=255)
                                                                                                                           gCAATAACGCACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAACGACGTAGTCCAGACCCCCGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAc                      <  2:992762/149‑1 (MQ=255)
                                                                                                                           gCAACAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAGGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAc                      <  2:741586/149‑1 (MQ=255)
                                                                                                                             aaTAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcgc                    >  2:264454/1‑149 (MQ=255)
                                                                                                                               tAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGa                  >  2:539499/1‑149 (MQ=255)
                                                                                                                               tAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGGCTGCATGTAGCGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcgggg                  >  2:31003/1‑146 (MQ=255)
                                                                                                                                   gTACGGGCAAATGAACTTCGTGGCGAGAAGCGAAATCGACTCATGCTTTAGAGCCGTCCGGTACAAAGGCGTAGCCAAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCCCAGACGCGAAATc              >  1:170233/1‑149 (MQ=255)
                                                                                                                                    tACGGGCAAATGGACTTCGTGGCGAGAAGCGCAAGCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCt             >  2:772245/1‑149 (MQ=255)
                                                                                                                                     aCGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGGCGTAGCCCAGAGCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCCCCATGCGGCGCAGCAGCGCAACCTg            >  2:202807/1‑149 (MQ=255)
                                                                                                                                     aCGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGACCAGACGCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTg            >  2:751828/1‑149 (MQ=255)
                                                                                                                                          cAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCCTGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGt       <  2:1026845/149‑1 (MQ=255)
                                                                                                                                             aTGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGGGCTGGATCTTCTTCCACCATGCGGCGCAGGCGGGAAATCTGCACGTCgt    >  2:362104/1‑148 (MQ=255)
                                                                                                                                             aTGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCGGACGCGAAATCTGCACGTCGa    <  2:272185/149‑1 (MQ=255)
                                                                                                                                             aTGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGa    <  2:572296/149‑1 (MQ=255)
                                                                                                                                               gAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATg  >  1:745806/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATG  >  CP009273/3529053‑3529344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: