Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,632,525 T→C 2.9% intergenic (+24/+220) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,632,5250TC 2.9% 547.4 / 2.7 196intergenic (+24/+220)pitA/uspBphosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base T (93/95);  new base C (2/5);  total (96/100)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 199 total aligned reads displayed.

TGGTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAA  >  CP009273/3632377‑3632668
                                                                                                                                                    |                                                                                                                                               
tggtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGt                                                                                                                                                 <  1:1004786/149‑1 (MQ=255)
 ggtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTc                                                                                                                                                >  2:627883/1‑149 (MQ=255)
    aGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAACCGCGCGGGTCAGc                                                                                                                                             >  2:37035/1‑149 (MQ=255)
       agctggCGGCGTACTGCGTAGAACTGTGTCAAAAATTTTCTGGACCGGCGTGTTTATCCGTCCGGCTTCTGTACTGCTTTCCGGCGGGCTGTTCTCGCTTTCCTGTCAGTTCCCGAAATGGTACGCACCAAGACAAACAGGTCAgttta                                                                                                                                          >  2:577061/4‑145 (MQ=255)
        ggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgga                                                                                                                                         <  2:216947/149‑2 (MQ=255)
         gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGcc                                                                                                                                        >  1:777616/1‑149 (MQ=255)
         gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGcc                                                                                                                                        <  1:1155221/149‑1 (MQ=255)
          tggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCACAACGAGCGGGTCAGCGCGccc                                                                                                                                       >  2:672193/1‑149 (MQ=255)
            gcgggTTATAGGGGAAAGCCGTGACCGGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGc                                                                                                                                     <  2:1108684/149‑1 (MQ=255)
              ggccgaCAGCGTAAAACCGTGCCGCGCATTCCGAGGGCCTGGGTGGTTGCCCTCCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCtt                                                                                                                                   <  1:1090106/144‑1 (MQ=255)
              ggcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCtt                                                                                                                                   <  1:57720/149‑1 (MQ=255)
               gcctACAGCGTAAACCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCCGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGCCAGCTGACCCGCtcg                                                                                                                                  <  2:1133322/146‑3 (MQ=255)
               gcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGCCAGCTGACCCGCtcg                                                                                                                                  <  2:643918/149‑3 (MQ=255)
                 ttACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTCCTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAg                                                                                                                                <  2:539417/149‑1 (MQ=255)
                  tACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCCTccgc                                                                                                                               >  2:295062/1‑146 (MQ=255)
                    cAGCGTAAAACCGTGCCCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAtt                                                                                                                             <  2:863919/149‑1 (MQ=255)
                    cAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCCGAtt                                                                                                                             >  2:1173288/1‑149 (MQ=255)
                    cAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAtt                                                                                                                             <  1:1229714/149‑1 (MQ=255)
                     aGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATtg                                                                                                                            <  1:940098/149‑1 (MQ=255)
                     aGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACCCACCAAAACCAGCGGGTCCGCTGGCCCGCTTccgctgg                                                                                                                            >  1:1151021/1‑143 (MQ=255)
                       cgcaaaaCCGTCACCAGCATTCTGAGGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATtgtg                                                                                                                          <  2:553414/146‑1 (MQ=255)
                       cGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCCGTTCCTGTAATCGTGCGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCCGATtgtg                                                                                                                          >  2:325022/1‑149 (MQ=255)
                         tAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTGCTGCTTGCCGGCGGGCTGTACTGGCGCTCCTTGCAGTTCCTGTAATCCTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGAc                                                                                                                        >  2:512548/1‑149 (MQ=255)
                             aCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAg                                                                                                                    >  2:715796/1‑149 (MQ=255)
                                 tgacacgCATCCTGATGGCCTGGGTGGTTACCCTTCCGGCGGCGGTACTACTTTCCGGCGGGCCAGACAGGCGCGCCTTGACGTACCCGTAAACGTCAGCACCGACACGAGCGGGTCAGCTGGCCCGCGTCAGATTGTGACAGAGTgcg                                                                                                                >  2:490028/7‑149 (MQ=255)
                                 tGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTgcg                                                                                                                >  2:168956/1‑149 (MQ=255)
                                 tGACCACTATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTgcg                                                                                                                <  1:456551/149‑1 (MQ=255)
                                      aGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTCCAGATTGTGACATAGTGCGCTTTg                                                                                                           >  1:805035/1‑149 (MQ=255)
                                       gCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGt                                                                                                          >  2:483858/1‑149 (MQ=255)
                                        cagtgtggtggcgagggTGTTTTCCCTTCCGGCTGAGGTCCTGCTGTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGTACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGtt                                                                                                         <  2:130361/135‑1 (MQ=255)
                                          ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                       <  1:1132586/149‑1 (MQ=255)
                                          ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                       <  2:214193/149‑1 (MQ=255)
                                          ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                       <  2:344340/149‑1 (MQ=255)
                                             tGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGc                                                                                                    >  1:208163/1‑149 (MQ=255)
                                               aTGGGCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCCGTACTGGCTCGCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCg                                                                                                  <  2:1002112/149‑1 (MQ=255)
                                               aTGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCg                                                                                                  <  2:256739/149‑1 (MQ=255)
                                               aTGGCCTGGGTGCTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGAGATAGTGCGCCTTGTTCATGCCg                                                                                                  <  2:11600/149‑1 (MQ=255)
                                                  gCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTACTCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGAt                                                                                               <  1:568947/149‑1 (MQ=255)
                                                    cTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACAGATTGCGCTTTGTTCATCCCGGTTgc                                                                                             >  1:1030045/1‑149 (MQ=255)
                                                      ggttgtTTCCCCTTCCGGCTGCGGTACTGCTTTCCGGTGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGCCCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATgcgg                                                                                           <  2:911387/146‑1 (MQ=255)
                                                        gtgtTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATgcggcg                                                                                         >  2:310487/1‑149 (MQ=255)
                                                         tgtTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGt                                                                                        <  1:1109809/149‑1 (MQ=255)
                                                           tttACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGGGTGa                                                                                      >  1:141264/1‑149 (MQ=255)
                                                           tttACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTCGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGCCAGCTGGCCCGCTCCAGATTGTGACATAGCGCGCTCTGTTCATGCCGGATGCGCCGTGa                                                                                      >  2:284555/1‑149 (MQ=255)
                                                             tccccTTCCGGCGGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGGGCGGGTCAGCTGGCCCGCTTCAGATTGTGACACAGTGCGCTTTGTTCATGCCGGATGCGGCGTTAAc                                                                                    <  1:352044/147‑1 (MQ=255)
                                                             tccccGTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTCCAGTTCCTGAAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATAAGGCGttcac                                                                                    <  1:884593/147‑5 (MQ=255)
                                                                ccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                 >  1:448408/1‑149 (MQ=255)
                                                                ccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTCCAGAGTGTGACATAGTGCGCTTTGTTCACGCCGCATCCGGCATGAAcaaa                                                                                 <  2:933102/149‑4 (MQ=255)
                                                                ccTTCCGGCTGCGGTACTGCNTNCCGNCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                 <  1:855259/149‑1 (MQ=255)
                                                                  ttCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                               >  1:363487/1‑149 (MQ=255)
                                                                  ttCCGGCTGCGGTACTCCTTTCCGACGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATTCCGGATGCGGCGTAGACGACtt                                                                               >  2:836786/1‑149 (MQ=255)
                                                                      ggCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc                                                                           <  2:389263/149‑1 (MQ=255)
                                                                      ggCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACACAGTGCGCGTTGTTCATGCCGGGTGCGGCGTGAACGCCTTATcc                                                                           >  2:745268/1‑149 (MQ=255)
                                                                       gCTGCGGTACTGCGTTCCGGCGGGCGGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                          <  1:761269/149‑1 (MQ=255)
                                                                         tGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                        >  1:767660/1‑149 (MQ=255)
                                                                         tGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                        <  1:232124/149‑1 (MQ=255)
                                                                          gCGGTCCTCCTTTCCGGCGGGCTGTACTGGCTGTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                       <  2:651044/149‑1 (MQ=255)
                                                                          gCGGGACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAGTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                       <  2:1040767/149‑1 (MQ=255)
                                                                           cGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGATTTGTTCATGCCGGATGCGGCGGGAACGCCTTATCCGGCCt                                                                      >  1:214151/1‑149 (MQ=255)
                                                                                  gCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaaa                                                               <  1:158066/149‑1 (MQ=255)
                                                                                  gCTTTCCGGCGGGCTGGACTGGCTCTCCGTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACCTAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaaa                                                               <  1:345174/149‑1 (MQ=255)
                                                                                  gCTTTCCGCCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGGAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaaa                                                               <  1:191687/149‑1 (MQ=255)
                                                                                   cgttCCGGCGGGCTGCACTGGCTCTCCTTGCAGTCCCTTGACCCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAAc                                                              <  1:842259/147‑1 (MQ=37)
                                                                                     ttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACAt                                                            <  1:295062/149‑1 (MQ=255)
                                                                                     ttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACAt                                                            <  1:279462/149‑1 (MQ=255)
                                                                                      tCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATg                                                           <  1:715796/149‑1 (MQ=255)
                                                                                        cggcggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCa                                                         <  1:259959/149‑1 (MQ=255)
                                                                                              gCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCa                                                   <  1:1211188/149‑1 (MQ=255)
                                                                                               cTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAACGGCTCCGCTGGCCCGCTTCAGCTTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCaa                                                  >  2:430698/1‑149 (MQ=37)
                                                                                               cTGTACTGGCTCTCCTTGCAGTCCCCGTAATCGTACGCACCAAAACGAGAGGGTCAGCTGGCCCGCTTCCGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGACAGCCTGATCCGGCCTACAAACACATGCAAACTCaa                                                  >  2:883678/1‑149 (MQ=37)
                                                                                                  tACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAg                                               >  2:59258/1‑149 (MQ=255)
                                                                                                  tACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAg                                               >  1:239058/1‑149 (MQ=255)
                                                                                                      ggCTCTGCTTGCAATTCCTGAAATCGTACGCACCAAAACGAGCGGGTCAGTTGGACCGCTTCAGATTGTGACCTAGTGCGCTTTGTTCATGTCGGATGCGGCGTGAACGTATTAAAAAAAAAACAAAAACATGCAAATTCAATAGATTg                                           <  2:822733/149‑1 (MQ=255)
                                                                                                      ggCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAACCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTg                                           <  1:604509/149‑1 (MQ=255)
                                                                                                       gCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGc                                          >  2:1095661/1‑149 (MQ=255)
                                                                                                        ctctCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                         >  1:386056/1‑149 (MQ=255)
                                                                                                        ccctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                         <  1:253/147‑1 (MQ=255)
                                                                                                         tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGGCATTGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGGCTACGAAAACATGCAAATTCAATAGATTGCag                                        >  2:622975/1‑149 (MQ=37)
                                                                                                         tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCag                                        >  2:856529/1‑149 (MQ=255)
                                                                                                         tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGGGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCag                                        >  1:1169761/1‑149 (MQ=255)
                                                                                                           tcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCATCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCagag                                      <  1:720709/149‑1 (MQ=255)
                                                                                                            ccTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATGCAATAGATTGCagggc                                     >  1:119381/1‑146 (MQ=255)
                                                                                                            ccTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTGGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCagagg                                     >  2:1079454/1‑148 (MQ=255)
                                                                                                            ccTTGCAGTTCCTGTAATCGTACGAACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGGTGAGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATGCAATAGATAGCAgaga                                     >  2:240156/1‑149 (MQ=37)
                                                                                                              ttGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCagagtgt                                   >  1:532230/1‑146 (MQ=255)
                                                                                                                  aGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTACAACTTGTGACATAGGGCGCCTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGGAAATTCAATGGTTTGCAGCGATCATGt                               >  2:787880/1‑149 (MQ=25)
                                                                                                                   tttCCCGTAATCGTACGCACCAAAACGAGCGGGTCCGCTGGCCCGCTTCAGATTGGGACATAGTGCGCTTTGTTCATGCAGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTa                              <  1:70435/148‑1 (MQ=255)
                                                                                                                   gTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTa                              <  1:321391/149‑1 (MQ=255)
                                                                                                                        tGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCt                         >  2:770204/1‑149 (MQ=255)
                                                                                                                        tGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCt                         >  2:726312/1‑149 (MQ=255)
                                                                                                                               gTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAACACCATGCAACTTCAATCGATTGCAGAGATTACGTAGGCTTGATAAGc                  <  2:1225127/149‑1 (MQ=25)
                                                                                                                               gTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGAGAAGc                  >  1:931097/1‑149 (MQ=255)
                                                                                                                                  cGCACCAAAACGGGCGGTTCAGCTGGCCCGCTTCTGTTTTTGACATAGTCCGCTTTGTTCATGCCGGATGCGGCCTGAACGCCTTATCCGGCCTACAGAAACATGTAAATTCAATACATTGCAGAGATGATGTAGGCCTGATAAGCGTa               <  2:414227/149‑1 (MQ=18)
                                                                                                                                   gCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAGCCGGCCTACAAAAACATGCAAATTCAATATATTGCAGAGATTATGTAGGCCTGATAAGCGTAg              <  1:907326/149‑1 (MQ=255)
                                                                                                                                    cACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgc             <  2:1091059/149‑1 (MQ=255)
                                                                                                                                     aCCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg            <  1:483858/149‑1 (MQ=255)
                                                                                                                                      ccAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTATGTTGATTCTGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAACTTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcgc           >  1:967302/1‑149 (MQ=25)
                                                                                                                                        aaaaCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAAATTGCCGAGATTATGTAGGCCTNATAAGCGTGGCGCAt         >  1:981911/1‑149 (MQ=255)
                                                                                                                                         agaCGGGCGGGTCAGCGGGGCCGCGTCAGATGGTGGCATAGTGCGCTTTGTTGATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATc        <  1:1024899/147‑1 (MQ=18)
                                                                                                                                           aCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCGGCAGGCGTCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAg      <  1:595333/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGGTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAA  >  CP009273/3632377‑3632668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: