Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,632,532 G→A 3.1% intergenic (+31/+213) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,632,5320GA 3.1% 578.2 / 5.4 196intergenic (+31/+213)pitA/uspBphosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base G (94/95);  new base A (2/4);  total (96/100)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-01

Only 100 of 199 total aligned reads displayed.

GGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTG  >  CP009273/3632385‑3632679
                                                                                                                                                   |                                                                                                                                                   
ggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgga                                                                                                                                                    <  2:216947/149‑2 (MQ=255)
 gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCCCCAAAACGAGCGGGTCAGCTGGcc                                                                                                                                                   >  1:598747/1‑149 (MQ=255)
    gcggtTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGc                                                                                                                                                <  2:83121/149‑1 (MQ=255)
      ggccgaCAGCGTAAAACCGTGCCGCGCATTCCGAGGGCCTGGGTGGTTGCCCTCCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCtt                                                                                                                                              <  1:1090106/144‑1 (MQ=255)
       gcctACAGCGTAAACCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCCGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGCCAGCTGACCCGCtcg                                                                                                                                             <  2:1133322/146‑3 (MQ=255)
         ttACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTCCTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAg                                                                                                                                           <  2:539417/149‑1 (MQ=255)
          tACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCCTccgc                                                                                                                                          >  2:295062/1‑146 (MQ=255)
            cAGCGTAAAACCGTGCCCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAtt                                                                                                                                        <  2:863919/149‑1 (MQ=255)
            cAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCCGAtt                                                                                                                                        >  2:1173288/1‑149 (MQ=255)
            cAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAtt                                                                                                                                        <  1:776762/149‑1 (MQ=255)
             aGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATtg                                                                                                                                       <  1:940098/149‑1 (MQ=255)
               cgcaaaaCCGTCACCAGCATTCTGAGGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATtgtg                                                                                                                                     <  2:553414/146‑1 (MQ=255)
               cGTAATACCGTGATCAGCATTCTGTTGGCCTGGGTGTTTACCCTTTGGGGTGCGGTACTGCTTTGCGGCGGGCTGTCCTGGCTCTCCTTGAAGTGCCTGTAATCGTACGCACCGAACCGAGCGGGTCAGCTGGCCCGCTTCTGTTtgtg                                                                                                                                     >  2:756009/1‑149 (MQ=255)
               cGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCCGTTCCTGTAATCGTGCGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCCGATtgtg                                                                                                                                     >  2:325022/1‑149 (MQ=255)
                  aaaaCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACa                                                                                                                                  <  1:134302/149‑1 (MQ=255)
                   aaaCCGTGACCAGCCTTCTGATGGCCTGGGTTTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGGCTTGGCTATCCTTGGGGAGCACGTGATGGCGATCGCCTAAACGAGCGGGGCGGCTGGCCCGCTTCAGATTGGGAcct                                                                                                                                 >  2:70435/1‑147 (MQ=255)
                         tGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTgcg                                                                                                                           >  2:168956/1‑149 (MQ=255)
                         tGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCCGGCCCGCTTCAGATTGTGACATAGTgcg                                                                                                                           >  2:718578/1‑149 (MQ=255)
                         tGACCACTATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTgcg                                                                                                                           <  1:456551/149‑1 (MQ=255)
                           aCCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCCTCAGATTGTGACACAGTgcgcc                                                                                                                         >  2:1086346/1‑148 (MQ=255)
                           aCCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGATCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGTCCCGCTTCAGATTGTGACATAGTGCGCt                                                                                                                         <  1:748199/149‑1 (MQ=255)
                                cagtgtggtggcgagggTGTTTTCCCTTCCGGCTGAGGTCCTGCTGTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGTACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGtt                                                                                                                    <  2:130361/135‑1 (MQ=255)
                                  ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCCCCAAAACGAGCGGGTCGGCTGGCCCCCTGCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                                  >  2:64760/1‑149 (MQ=255)
                                  ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                                  <  2:214193/149‑1 (MQ=255)
                                  ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                                  >  1:573306/1‑149 (MQ=255)
                                  ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCa                                                                                                                  <  2:344340/149‑1 (MQ=255)
                                   tCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGGGCGGGTCAACTGGCCCGCTTCAGATTGTGACATAGGGCGCTTTGTTCAt                                                                                                                 >  1:1155077/1‑149 (MQ=255)
                                       aTGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCg                                                                                                             <  2:256739/149‑1 (MQ=255)
                                       aTGGCCTGGGTGCTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGAGATAGTGCGCCTTGTTCATGCCg                                                                                                             <  2:11600/149‑1 (MQ=255)
                                           ccTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATg                                                                                                         >  2:595333/1‑149 (MQ=255)
                                            cTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCTTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACAGAGTGCGCTTTGTTCATGCCGGATgc                                                                                                        >  1:763615/1‑149 (MQ=255)
                                              ggttgtTTCCCCTTCCGGCTGCGGTACTGCTTTCCGGTGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGCCCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATgcgg                                                                                                      <  2:911387/146‑1 (MQ=255)
                                               ggtggtTACCCTTCCGGCTGCGGTACTGCTTTCCGGCTGGCTGTACTGGCTCTCCATGCCGTTCCGGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAAATGGTGACGTTGCCGGCTTTGTTCATGCCAGATgcggc                                                                                                     >  2:387994/1‑149 (MQ=255)
                                                gtgtTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATgcggcg                                                                                                    >  2:310487/1‑149 (MQ=255)
                                                   tttACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTCGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGCCAGCTGGCCCGCTCCAGATTGTGACATAGCGCGCTCTGTTCATGCCGGATGCGCCGTGa                                                                                                 >  2:284555/1‑149 (MQ=255)
                                                    ttACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCACGCCGCATCCGGCATGaa                                                                                                <  1:1119063/149‑1 (MQ=255)
                                                     tccccTTCCGGCGGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGGGCGGGTCAGCTGGCCCGCTTCAGATTGTGACACAGTGCGCTTTGTTCATGCCGGATGCGGCGTTAAc                                                                                               <  1:352044/147‑1 (MQ=255)
                                                       cccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCCCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                             <  1:180268/149‑1 (MQ=255)
                                                        ccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCTGCGTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                            <  1:853833/149‑1 (MQ=255)
                                                        ccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                            >  1:448408/1‑149 (MQ=255)
                                                        ccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTCCAGAGTGTGACATAGTGCGCTTTGTTCACGCCGCATCCGGCATGAAcaaa                                                                                            <  2:933102/149‑4 (MQ=255)
                                                          ttCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                                          >  1:363487/1‑149 (MQ=255)
                                                               gCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGGGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                     <  1:149028/149‑1 (MQ=255)
                                                               gCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                     <  1:1025240/149‑1 (MQ=255)
                                                               gCTGCGGGACTGCTTGCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                     <  2:494821/149‑1 (MQ=255)
                                                                 tGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                   <  1:232124/149‑1 (MQ=255)
                                                                   cGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGATTTGTTCATGCCGGATGCGGCGGGAACGCCTTATCCGGCCt                                                                                 >  1:214151/1‑149 (MQ=255)
                                                                   cGGTACTGCTTTCCGGCGGGCTGTACTGGCTCACGACACAGGTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                 >  1:1225144/1‑149 (MQ=38)
                                                                          gactgccGGCGGGCGGGTCTGGCGCTCCTTGCGGTTCCTGTAATCGTACTCACCAAAACGGGCTGGCCCCCTCGATCGCGTTAGTTTTGGATATACTGCGCTTTGTTCAGGCCGGATGCGGCGTGATCTCCTTATCCGGCCTACaaaaa                                                                          <  2:445726/144‑1 (MQ=12)
                                                                          gCTTTCCGGCGGGCTGGACTGGCTCTCCGTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACCTAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaaa                                                                          <  1:345174/149‑1 (MQ=255)
                                                                            tttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACa                                                                        <  2:415493/149‑1 (MQ=255)
                                                                             ttCCGGGCGGCTGGACTGGCTCTCCTTTCGTGTTCTGGAATCGGCCGCCCCAAGACGAGCGGGTCAGCGGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACAt                                                                       <  2:654815/149‑1 (MQ=21)
                                                                             ttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACAt                                                                       <  1:279462/149‑1 (MQ=255)
                                                                             ttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACAt                                                                       <  1:295062/149‑1 (MQ=255)
                                                                              tCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATg                                                                      <  1:715796/149‑1 (MQ=255)
                                                                                cggcggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCa                                                                    <  1:259959/149‑1 (MQ=255)
                                                                                cggcggGCCGTACTGGCTCTCCTTGCCGTTCCTCTATTCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCa                                                                    <  1:841018/149‑1 (MQ=255)
                                                                                       cTGTACTGGCTCTCCTTGCAGTCCCCGTAATCGTACGCACCAAAACGAGAGGGTCAGCTGGCCCGCTTCCGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGACAGCCTGATCCGGCCTACAAACACATGCAAACTCaa                                                             >  2:883678/1‑149 (MQ=37)
                                                                                        tGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCGAAATCaag                                                            >  2:1094634/1‑148 (MQ=255)
                                                                                         gTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATa                                                           <  2:1040842/149‑1 (MQ=255)
                                                                                          tACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAg                                                          >  1:782821/1‑149 (MQ=255)
                                                                                          tACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAg                                                          >  1:239058/1‑149 (MQ=255)
                                                                                           aCTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGa                                                         <  1:588101/149‑1 (MQ=255)
                                                                                           aCTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGa                                                         <  1:1171307/149‑1 (MQ=255)
                                                                                            cTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGAAGGCTCTATTTTTATCGCCTTATCCGGCCTACAATAACATGCAAATTCAATAGAt                                                        >  1:1208160/1‑149 (MQ=255)
                                                                                                ctctCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                    >  1:386056/1‑149 (MQ=255)
                                                                                                ctctCCTGGCAGCTCCGGTAATCGTACGCACCAAAACCAGCGGGTCAGCTGGCCCGCCTCAGATTGGGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                    <  2:8450/149‑1 (MQ=25)
                                                                                                ccctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                    <  1:253/147‑1 (MQ=255)
                                                                                                 tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCag                                                   >  2:489738/1‑149 (MQ=255)
                                                                                                  ctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCaaa                                                  >  2:794311/1‑147 (MQ=255)
                                                                                                   tcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGGCGGATGCGGCGTGAACGCCTTCTCCGGCCTACCAAAACATGTCACGTCAATAGATTGctagt                                                 >  1:196753/1‑145 (MQ=37)
                                                                                                   tcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCATCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCagag                                                 <  1:720709/149‑1 (MQ=255)
                                                                                                    ccTTGCAGTTCCTGTAATCGTACGAACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGGTGAGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATGCAATAGATAGCAgaga                                                >  2:240156/1‑149 (MQ=37)
                                                                                                          aGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTGCAAAAACATGCAATTTCTATTGATTGCAGGGGTTATGt                                          >  1:534119/1‑149 (MQ=37)
                                                                                                           tttCCCGTAATCGTACGCACCAAAACGAGCGGGTCCGCTGGCCCGCTTCAGATTGGGACATAGTGCGCTTTGTTCATGCAGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTa                                         <  1:70435/148‑1 (MQ=255)
                                                                                                           gTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTa                                         <  1:321391/149‑1 (MQ=255)
                                                                                                             tCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAgg                                       >  2:406167/1‑149 (MQ=255)
                                                                                                                     tagTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGGGCGTGACATCGTGCGCTTTGTTCCTGCGGGATGCGTCGTGAACGCCTTATCCGGCCTACACAAACATGCGACATCAATAGACTGCTGAGATGCTGTAGGCCTGaaaa                               >  2:1029446/3‑149 (MQ=21)
                                                                                                                     tCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACAGAGTCAACTTGGTGCAGGCCGGAGGCGGCGTGAACGCCTTATCAGGCCTCCAAAAACATGCAAATTCAATGGGTTGCGCAGGTTCGGTAGGccgggtga                               >  2:624014/1‑143 (MQ=255)
                                                                                                                       gTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAACACCATGCAACTTCAATCGATTGCAGAGATTACGTAGGCTTGATAAGc                             <  2:1225127/149‑1 (MQ=25)
                                                                                                                       gTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGAGAAGc                             >  1:931097/1‑149 (MQ=255)
                                                                                                                           gCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAGCCGGCCTACAAAAACATGCAAATTCAATATATTGCAGAGATTATGTAGGCCTGATAAGCGTAg                         <  1:907326/149‑1 (MQ=255)
                                                                                                                             aCCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg                       >  1:882183/1‑149 (MQ=255)
                                                                                                                             aCCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg                       >  2:745196/1‑149 (MQ=255)
                                                                                                                             aCCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg                       <  1:483858/149‑1 (MQ=255)
                                                                                                                              ccAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTATGTTGATTCTGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAACTTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcgc                      >  1:967302/1‑149 (MQ=25)
                                                                                                                               cAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAACATGCAAATTCACTAGATTGCAGAGCGTATGTAGGCCTGATAAGCGTAGCGCa                     >  2:1120244/1‑149 (MQ=255)
                                                                                                                               cAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCa                     >  2:781467/1‑149 (MQ=255)
                                                                                                                                aaaaCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAAATTGCCGAGATTATGTAGGCCTNATAAGCGTGGCGCAt                    >  1:981911/1‑149 (MQ=255)
                                                                                                                                 agaCGGGCGGGTCAGCGGGGCCGCGTCAGATGGTGGCATAGTGCGCTTTGTTGATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATc                   <  1:1024899/147‑1 (MQ=18)
                                                                                                                                  aaCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCa                  >  2:1103824/1‑149 (MQ=255)
                                                                                                                                     gAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCAATAGGCGGCTTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGc               >  1:1164098/1‑149 (MQ=25)
                                                                                                                                     aaGCGGGCCAGCTGACCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGc               >  1:1140862/2‑149 (MQ=255)
                                                                                                                                             cggttGGCCCGCTTCAGAGTTTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTTGCGCATCAGGCAATTTTGc       <  2:515794/145‑1 (MQ=255)
                                                                                                                                              aGCTGGCCCGCTTCAGTTTGTGACTTAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCg      <  2:856815/149‑1 (MQ=255)
                                                                                                                                              aGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCg      >  1:1038839/1‑149 (MQ=255)
                                                                                                                                                 tGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGttt   <  1:660053/149‑1 (MQ=255)
                                                                                                                                                 tGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGttt   >  1:999645/1‑149 (MQ=255)
                                                                                                                                                  ggCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTg  <  2:573306/149‑1 (MQ=255)
                                                                                                                                                  ggCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTg  <  1:497215/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTG  >  CP009273/3632385‑3632679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: