Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,726,790 T→A 5.4% N410K (AAT→AAA xylG → fused D‑xylose transporter subunits of ABC superfamily: ATP‑binding components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,726,7900TA 5.4% 455.9 / 2.6 160N410K (AAT→AAAxylGfused D‑xylose transporter subunits of ABC superfamily: ATP‑binding components
Reads supporting (aligned to +/- strand):  ref base T (75/73);  new base A (2/8);  total (77/83)
Fisher's exact test for biased strand distribution p-value = 9.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 163 total aligned reads displayed.

TTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCGTTATT  >  CP009273/3726642‑3726937
                                                                                                                                                    |                                                                                                                                                   
ttACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAAt                                                                                                                                                     >  2:758596/1‑149 (MQ=255)
tgaccgctgcctcACGCAATACATTTACCGGTGGCATGAGCCAGCTGGATGACGCGGCAGAGCAAAAATGTATTTTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAAt                                                                                                                                                     <  2:855983/141‑1 (MQ=255)
      tCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAACGTTAAAACGTCGTCCCCCGACCTTACTATAGAACGCTTGAGCGTCGGCACTCAGCaa                                                                                                                                               >  2:556956/1‑149 (MQ=255)
       cgccgcACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCaaa                                                                                                                                              <  1:188740/149‑1 (MQ=255)
         ccgcACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCaaaaa                                                                                                                                            >  2:481590/1‑149 (MQ=255)
         ccgcACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCaaaaa                                                                                                                                            >  2:387327/1‑149 (MQ=255)
          cgcACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGAACCTGACTGTTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCaaaagc                                                                                                                                           <  1:1213361/149‑3 (MQ=255)
          cgcACTCAATAAATTTACCGGTGCCATTAGCACGCTTGATGGCACGGCAGATCAAAATTTTATTCTGTAATCAAGCCTTCAGCTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAg                                                                                                                                           <  2:723278/149‑1 (MQ=255)
           gcACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGc                                                                                                                                          <  1:351087/149‑1 (MQ=255)
            cACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAATACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCg                                                                                                                                         <  1:722104/149‑1 (MQ=255)
               tCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATc                                                                                                                                      <  1:488555/149‑1 (MQ=255)
                cAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATCGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATcc                                                                                                                                     >  1:552066/1‑149 (MQ=255)
                 aaTAAATGTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGGTCct                                                                                                                                    >  2:699472/1‑149 (MQ=255)
                    aaaTTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCctcgc                                                                                                                                 >  2:906694/1‑149 (MQ=255)
                    aaaTTGACCGGTGGCATTAGCCAGCTTGATGACGAGGCAGAGAAAAAATGTATTCTGGAATCAAACCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGAGAGAAAAAAAAAAAAGCGATCctcgc                                                                                                                                 <  2:845047/149‑1 (MQ=255)
                      aTTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCctcgctc                                                                                                                               >  1:983732/1‑149 (MQ=255)
                           ccGGTGGCATTAGCCAACTTGATGACACGGCAGAGCAGAAATGTAATCTGGAATCAATCCCGCCAAACAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGCCGGCAAACAGCAAAAAGCGATCCCCGCACGctgt                                                                                                                          >  1:785897/1‑149 (MQ=255)
                             ggTGGCATTCGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAACGCAATCctagccccccgtac                                                                                                                        >  2:1171003/1‑140 (MQ=255)
                               tGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGctgtctgt                                                                                                                      >  1:817949/1‑149 (MQ=255)
                                 tcATTAGGCAGCTTGATGACGCGGCAGAGCAAAAATGTATGCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCTCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTa                                                                                                                    <  2:952142/148‑1 (MQ=255)
                                 gCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAACGCGATCCTCGCTCGCTGTCTGTTa                                                                                                                    <  2:1196440/149‑1 (MQ=255)
                                    ttAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTCTTACtt                                                                                                                 >  1:12612/1‑149 (MQ=255)
                                     tAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTa                                                                                                                <  1:84197/149‑1 (MQ=255)
                                           gcgtgatgaCGCGTCAGAGCAAAAATGACTGCTGGAAACGAGAGAGACACGCAGAGATAAAACGTCGTCCCCCGACCTTTCTTTCGGGCGTTTGAGCGGCCGCAAACAGAAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCcgc                                                                                                          <  2:379982/146‑1 (MQ=255)
                                           gCTTGATGACGCGGTAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCAGAAAAAAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCcgc                                                                                                          <  2:434993/149‑1 (MQ=255)
                                            cTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCTCTCGCTGTCTGTTACTTAACCcgcg                                                                                                         <  1:620625/149‑1 (MQ=255)
                                              tgaggaCGGGGCAGAGCAAAAATGCATTCGGGAATCAATCCAGCACCTCAAAGTTAAAACGTCGGCCCCCGACCTGGCTATGGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGca                                                                                                       <  2:244509/149‑1 (MQ=255)
                                                atgaCGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAAACAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGcatt                                                                                                     >  2:1122236/1‑149 (MQ=255)
                                                aagaCGCGGCAGAGCAAAAATGAATTCTGGAATCACTCCAGCAACTCACAGTTCAACCGTCGTCACCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAACGATCCTCGCTCGCTGTCTCGTACTTAACCCGCCcata                                                                                                     >  2:159873/3‑148 (MQ=255)
                                                  gaCGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAGCTCAAAGTTAAACCGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAGTCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCAGCGgattct                                                                                                   >  1:204766/1‑149 (MQ=255)
                                                     gcgGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGcattctcat                                                                                                >  1:1107709/1‑149 (MQ=255)
                                                        gCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGcgttctccttct                                                                                             >  1:175856/1‑149 (MQ=255)
                                                        gCAGAGCAAAAACGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAACGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGcattctcattct                                                                                             <  2:226272/149‑1 (MQ=255)
                                                          agagCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtg                                                                                           <  1:1109224/149‑1 (MQ=255)
                                                          agagCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCAGTCTGTTACTTAACCCGCGCATTCCCATTCTtg                                                                                           <  1:906694/149‑1 (MQ=255)
                                                          agagCAAAAATGTATTCTGGAATAACTCCACGCCGTAAAAGTTTAAGCTTCTTCACCCAACCTTACAATTTTACGTTGAAGCGTCTGCAATCATCAAAATGCTATCCTCTCTCTCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtg                                                                                           <  2:1031353/149‑1 (MQ=255)
                                                          agacCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGTCCTTGCTATTGGCCGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtg                                                                                           <  1:1129985/149‑1 (MQ=255)
                                                           gagCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtga                                                                                          <  1:387327/149‑1 (MQ=255)
                                                            cgCAAAAATGTATTCTGGAATCAATCCAGCACCCCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCAAAAAAAAACAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtgat                                                                                         <  2:758127/148‑1 (MQ=255)
                                                            agCAAAAATGTATTCTGGAATCAATCCAGCACCTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtgat                                                                                         <  1:566503/149‑1 (MQ=255)
                                                              cAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCGCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTtgatga                                                                                       >  2:1129614/1‑149 (MQ=255)
                                                               aaaaaTGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTCGCCCGCGCATTCTCATTATTGAGGaa                                                                                      >  1:895133/1‑149 (MQ=255)
                                                                aaaaTGCATTCTGGAATCAAGCTAGCAACTCAAAGTGAAAACGTCGTCCCGCGACCTTGCTATTGGACGTTTGAGCGGCGACAATAAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAAc                                                                                     <  2:652203/149‑1 (MQ=255)
                                                                    tgcaTTCGGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACccac                                                                                 <  2:725080/146‑1 (MQ=255)
                                                                       aTTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTGGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCCCATTCTCATCCTTGATGACCCCACCAg                                                                              >  2:446575/1‑149 (MQ=255)
                                                                        ttCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATAAACCCACCAgg                                                                             >  1:506855/1‑149 (MQ=255)
                                                                         tCTGGAATCAATCCAGCAACTCAAAGTTACAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAggg                                                                            >  1:758310/1‑149 (MQ=255)
                                                                         tCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAggg                                                                            <  2:1057033/149‑1 (MQ=255)
                                                                           tGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGt                                                                          <  1:944155/149‑1 (MQ=255)
                                                                           tGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCCCATTCTTGATGAACCCACCAgggat                                                                          >  2:305049/1‑147 (MQ=255)
                                                                               atcaatcCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTAGCTTTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCg                                                                      >  1:901582/1‑149 (MQ=255)
                                                                                   atcCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGatat                                                                  >  1:362754/1‑149 (MQ=255)
                                                                                   atcCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGatat                                                                  <  1:580320/149‑1 (MQ=255)
                                                                                    tcCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTACCGATAtt                                                                 >  1:471849/1‑149 (MQ=255)
                                                                                     ccAGCCGCTCAAAGTTAACAGGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTg                                                                <  2:738610/149‑1 (MQ=255)
                                                                                     ccAGCATCTCAAATTTTTATCGTCGTCCCCCGGCCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTg                                                                <  1:149110/149‑1 (MQ=255)
                                                                                     ccAGCAACTCAAAGTTAAACCGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTg                                                                <  2:936426/149‑1 (MQ=255)
                                                                                     ccAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTg                                                                <  1:1188124/149‑1 (MQ=255)
                                                                                     ccAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTg                                                                >  2:426150/1‑149 (MQ=255)
                                                                                      cAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGAAATTgg                                                               >  1:508128/1‑149 (MQ=255)
                                                                                       aGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCCGCAAAAAGCGATCCTCGCTCGCTATCTGTGACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGgc                                                              >  2:444553/1‑149 (MQ=255)
                                                                                        tcAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGgcg                                                             <  2:293245/148‑1 (MQ=255)
                                                                                        gCAACGCAAAGTGAAAACGTCGTCCCCCGACCTTGCGATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGAGCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGgcg                                                             <  2:1143564/149‑1 (MQ=255)
                                                                                            cTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGaa                                                         >  2:655031/1‑149 (MQ=255)
                                                                                             tCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGaaa                                                        >  2:1213375/1‑149 (MQ=255)
                                                                                             tCAAAGGTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGGAATTAGCAAAAAGCGATCCTCACACGCTGTCTGTTACTTAACGCGCGCATTCACATTCTTGATGAACACACCAAGGGAAGCGATATTAGCACGaaa                                                        >  2:806116/1‑149 (MQ=255)
                                                                                                  gTTAAACCGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACga                                                   <  2:48966/149‑1 (MQ=255)
                                                                                                  gTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACga                                                   <  2:1205554/149‑1 (MQ=255)
                                                                                                  gTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACga                                                   <  2:830715/149‑1 (MQ=255)
                                                                                                    tAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACgaga                                                 <  1:731102/149‑1 (MQ=255)
                                                                                                    tAAAACGGCGCCCCCCGCCCTTGCGAGTGGACGGTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACgaga                                                 <  2:279696/149‑1 (MQ=255)
                                                                                                      aaaCGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATc                                               <  1:124993/149‑1 (MQ=255)
                                                                                                       aaCGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGCTATTGGCGCGAAATACGAGATCt                                              >  1:198840/1‑149 (MQ=255)
                                                                                                         cgtcgtCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTAc                                            <  1:301361/149‑1 (MQ=255)
                                                                                                             gtcCCCCGACCTTGCTATTGGACGTTTGGGCGGCGGCAATCAGCAAAAAGCCATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAaat                                        <  2:98831/149‑1 (MQ=255)
                                                                                                             gtcCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAaat                                        <  1:560162/149‑1 (MQ=255)
                                                                                                                ccccGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACGCACCAGGGGTATCGATATTGGCGCGAAATACGAGATGTACAaattaa                                     >  1:194378/1‑149 (MQ=255)
                                                                                                                ccccGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGCAATACGAGACCTACAaattaa                                     >  2:599944/1‑149 (MQ=255)
                                                                                                                ccccGACCTTGCGATTGGACGTTTGAGCGGCGGCAATCAGTAAAAAGCGATCCTCGCTCGCTGTCTTTTACTTAACCCGCTCATTCTCATTCTTGATGAACCCACCTTTTTTATTGATGTTGCGGCGACATCCGAGATCTACAcattaa                                     <  2:236042/149‑1 (MQ=255)
                                                                                                                      ccgtGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCCCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAAcc                               <  1:310311/146‑1 (MQ=255)
                                                                                                                         tGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAGAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAAc                            >  2:770308/1‑149 (MQ=255)
                                                                                                                         tGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTCCAAATTACTTAACCaat                            >  2:866569/1‑148 (MQ=255)
                                                                                                                          gCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGCTCCTCGCTCGCTGTCTGTTACTGAACCCTCTCGGTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATCCGAGATCTGCAAATTAATTAACCAACt                           >  2:876938/1‑149 (MQ=255)
                                                                                                                           cTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCGGGGGTGTCGGTCTTGGCGCGAAATACGAGAACGGACAATTAATTAACCAACTc                          >  1:459793/1‑149 (MQ=255)
                                                                                                                              ttGGCCGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTCTATGAACCCACTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACATTAATTAACCAACTCGTc                       <  2:453676/149‑1 (MQ=255)
                                                                                                                                 gACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACAACATCTTCcag                    >  1:975734/1‑149 (MQ=255)
                                                                                                                                  aCGTTTGAGCGTCGGCACTCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCcagc                   <  1:1177425/149‑1 (MQ=255)
                                                                                                                                    gTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAGATACGAGATCTACAAATTAATTAACCAACTCgacaagaaa                 >  1:698110/1‑141 (MQ=255)
                                                                                                                                    gTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCcagcag                 >  1:1021619/1‑149 (MQ=255)
                                                                                                                                      ttGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAggg               >  2:432635/1‑149 (MQ=255)
                                                                                                                                           cggcggCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTg          >  2:1222279/1‑149 (MQ=255)
                                                                                                                                              cggcAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATGGCCg       >  1:693682/1‑149 (MQ=255)
                                                                                                                                               ggcAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCGACAAATTAAATAACCAACTCATCaagcagaacaatgccgc      >  1:335225/1‑138 (MQ=255)
                                                                                                                                                gcAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAGATACGAGATCTACAAATTGATAAACCAACTCGTCCAGCAGGATATTGACgat     >  1:407247/1‑147 (MQ=255)
                                                                                                                                                gcAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACCCGTCCAGCAGGGTCTTGCCGtt     >  1:248734/1‑149 (MQ=255)
                                                                                                                                                gcAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGGATCGATATTGGCGCGAAATACGAGAACTACAAATTAAGTAACCAACTCGCCCAGCCGGGTATTGCCGtt     >  2:602047/1‑149 (MQ=255)
                                                                                                                                                gcAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGATACTTAACCCGCGCATTCTCGTCATTGATGACCCCTATATGGTTATCGATATTGGCGCGAAATACGAGAGCTTCAAATTAAGTAACCAACACGTCCAGCAGGGGATTGCCGtt     >  2:200225/1‑149 (MQ=255)
                                                                                                                                                  aaTCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATGCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTGCAGCTGGGTATTGCCGTTAt   >  2:805469/1‑149 (MQ=255)
                                                                                                                                                   aTCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTCCAAATTAATTAACCAACTCGCCCCGAAGGGTATTGCCGATagt  >  1:105534/1‑147 (MQ=255)
                                                                                                                                                   aTCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCTTCCAGCAGGGTATTGCCGTTAtt  <  1:651654/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAAAAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCGTTATT  >  CP009273/3726642‑3726937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: