Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,305,853 C→T D126N (GAC→AAC)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,8530CT98.7% 246.7 / NA 74D126N (GAC→AAC) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (43/30);  minor base A (1/0);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGA  >  CP009273/2305711‑2305992
                                                                                                                                              |                                                                                                                                           
ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCa                                                                                                                                       <  2:411461/149‑1 (MQ=255)
 tACCCTGGTACTGAACAGCAAAGTTAAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAg                                                                                                                                      >  1:660453/1‑149 (MQ=255)
   cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGa                                                                                                                                    <  1:39956/149‑1 (MQ=255)
    ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGaa                                                                                                                                   >  2:894650/1‑149 (MQ=255)
      tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCGCCGAATTCTGGCAGTACGTTGGTCCAGGAAGt                                                                                                                                 >  1:24125/1‑149 (MQ=255)
        gtccgtaaCAGCAAGGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTa                                                                                                                               <  1:688278/143‑1 (MQ=255)
           cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGt                                                                                                                            <  1:529892/149‑1 (MQ=255)
            tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTCCGTTGGTCCAGGCAGTTACGTc                                                                                                                           >  1:551153/1‑149 (MQ=255)
                   cAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTCCCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAacca                                                                                                                    >  2:7224/1‑149 (MQ=255)
                    aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAaacaa                                                                                                                   <  1:913978/149‑1 (MQ=255)
                    aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGCCAGTACGTTGGTCCAGGAAGTTACGTCATAaacaa                                                                                                                   >  2:403695/1‑149 (MQ=255)
                       gTTCAGGCCGTCAACCCGACCGAAGAAGGCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCCGGAAGTTACGTCATAATCAACGc                                                                                                                <  2:650936/149‑1 (MQ=255)
                          cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCGTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGGACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGt                                                                                                             >  1:994071/1‑149 (MQ=255)
                             gCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                                                                                          >  1:190746/1‑149 (MQ=255)
                             gCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                                                                                          >  1:566225/1‑149 (MQ=255)
                               cGTCAACCATACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGTTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                                                                                        <  1:96815/149‑1 (MQ=255)
                                     ccAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCTCCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg                                                                                                  <  2:263900/149‑1 (MQ=255)
                                     ccAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg                                                                                                  >  1:128103/1‑149 (MQ=255)
                                     ccAGACCGAAGAAGTCAGTGTTACAGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTATGCCAGTGCGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg                                                                                                  >  1:981981/1‑149 (MQ=255)
                                     ccAGAACGAAGAAATCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGGAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg                                                                                                  >  2:818203/1‑149 (MQ=255)
                                        gACCGAAGCAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg                                                                                               >  2:159784/1‑149 (MQ=255)
                                            gaagaaGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa                                                                                           <  1:955178/149‑1 (MQ=255)
                                            gaagaaGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGCCGa                                                                                           >  1:330327/1‑149 (MQ=255)
                                               gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                                                                                        >  1:134100/1‑149 (MQ=255)
                                                   tCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTGGTCGAAAGAAcc                                                                                    >  1:23408/1‑149 (MQ=255)
                                                   tCAGTGTTACGGTAGGGCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAcc                                                                                    <  1:1273017/149‑1 (MQ=255)
                                                    cAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTATGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAccc                                                                                   >  2:656021/1‑149 (MQ=255)
                                                    cAGTGTTACGGTAGGGCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAccc                                                                                   <  2:644190/149‑1 (MQ=255)
                                                     aGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCca                                                                                  <  1:1251242/149‑1 (MQ=255)
                                                       tgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCcaca                                                                                >  2:573844/1‑149 (MQ=255)
                                                            cGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg                                                                           <  2:672983/149‑1 (MQ=255)
                                                            cGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg                                                                           <  1:1063342/149‑1 (MQ=255)
                                                                  gTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt                                                                     >  1:616630/1‑149 (MQ=255)
                                                                  gTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAAGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt                                                                     >  2:990626/1‑149 (MQ=255)
                                                                   ccgcgAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACTACCGTAGTCTAAAGAACCCACATCCTGGAATTTc                                                                    <  1:672336/148‑1 (MQ=255)
                                                                      cgAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa                                                                 <  2:678790/149‑1 (MQ=255)
                                                                       gAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCCCATCCTGGAATTTCAGAc                                                                >  1:1196914/1‑149 (MQ=255)
                                                                        aaGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGaat                                                               >  2:658802/1‑147 (MQ=255)
                                                                               tACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAt                                                        <  2:838719/149‑1 (MQ=255)
                                                                               tACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAt                                                        <  1:74243/149‑1 (MQ=255)
                                                                               tACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAt                                                        <  1:754635/149‑1 (MQ=255)
                                                                                 ccACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGc                                                      <  1:397543/149‑1 (MQ=255)
                                                                                  cACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGcc                                                     >  2:1095798/1‑149 (MQ=255)
                                                                                  cACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGAGAATGcc                                                     >  2:927099/1‑149 (MQ=255)
                                                                                        gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCGGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg                                               <  2:459983/149‑1 (MQ=255)
                                                                                             aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTTCAGGAAGTTGCGTCATAAAAAGAGTCGCGGTTCCGGCCCTAGGTTAACGGTTCAATTTCCTGGAATTTCAGCCTTGCGAATGCCACACGGGTCCa                                          <  2:1270725/149‑1 (MQ=255)
                                                                                             aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCa                                          <  2:1232634/149‑1 (MQ=255)
                                                                                              ttaacttGTCAGAACCGCAGGTGTCACCCCCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAg                                         <  1:747778/144‑1 (MQ=255)
                                                                                              tGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGGGAATGCCCCACGGGGCCAg                                         >  2:1255087/1‑149 (MQ=255)
                                                                                              tGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgggccgac                                         >  2:1322295/1‑144 (MQ=255)
                                                                                              gttatgtttCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGCCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAg                                         <  2:660453/141‑1 (MQ=255)
                                                                                               gAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGCCCGATGGTCCAGGAAGTTCCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCCCCTCCTGGAATTTCAGACCTGCGAATGCCACCCGGAGCCAgg                                        >  2:824385/1‑149 (MQ=255)
                                                                                                  gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATCAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgt                                     >  1:38464/1‑149 (MQ=255)
                                                                                                  gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgt                                     >  2:90427/1‑149 (MQ=255)
                                                                                                   ttGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACCCGGGTCCAGGGgtt                                    >  1:566814/1‑149 (MQ=255)
                                                                                                   ttGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTCCGAATGCCACACGGTGCCGGGAgtg                                    >  1:278708/1‑148 (MQ=255)
                                                                                                   gtGTCAGCACCGGAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgtt                                    <  1:571433/148‑1 (MQ=255)
                                                                                                      tCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTCGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCGGGAgttgtt                                 >  2:257833/1‑149 (MQ=255)
                                                                                                        ggAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCAAGGAGTTgtttt                               >  1:118609/2‑149 (MQ=255)
                                                                                                         gAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACGGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGGGGTgttttc                              >  1:317205/1‑149 (MQ=255)
                                                                                                             cGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttcgttt                          >  2:1116597/1‑149 (MQ=255)
                                                                                                               tAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACTAGGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttcgttttc                        >  1:379397/1‑149 (MQ=255)
                                                                                                                aGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTGGTTTTCa                       >  1:258685/1‑149 (MQ=255)
                                                                                                                aGGTGTCAACACCGAATTCTGGCAGTACGTAGGTCAAGGAAGTTACGTCATAAAAAAGCACGTAGTTACGACAGAAACAGAAAGAACACACATCCTGGAATTTCAGACCTGCAAATGCCACAGGGGACCAGGCTTGGTTTTCGGTTTCa                       >  2:62535/1‑149 (MQ=255)
                                                                                                                         caccGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGACTGCCACACGGGTCCAGGGGTTGTTTTCGTTTTCAGCGCTGTTg              >  2:435572/1‑149 (MQ=255)
                                                                                                                         caccGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCCGGAGTTGTTTTCGTTTTCAGCGCTGTTg              >  2:722385/1‑149 (MQ=255)
                                                                                                                         caccGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTGTTCAGCGCTGGTg              >  1:623171/1‑149 (MQ=255)
                                                                                                                          accGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGc             <  1:821129/149‑1 (MQ=255)
                                                                                                                               aTTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCAAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTgg        >  1:765534/1‑149 (MQ=255)
                                                                                                                                ttCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGGGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGa       >  1:381137/1‑149 (MQ=255)
                                                                                                                                 tCTGGCAGTACGTTGGTCCAGGAAGCTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGTATTTCTGACCTGCGAATGCCACACGGGTCCAGGAGTTCTTTTCGTTTTCAGCGCTGTTGCCCTGGAt      <  1:813753/149‑1 (MQ=255)
                                                                                                                                   tGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCt    >  1:954423/1‑149 (MQ=255)
                                                                                                                                     gaaGTACGCTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGa  <  1:894650/147‑1 (MQ=255)
                                                                                                                                     gCAGTACGTTGGTCCAGGAAGTTACGTCATAAACACCGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGa  <  2:156258/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGA  >  CP009273/2305711‑2305992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑