Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,529,197	A→G	S11P (TCA→CCA)	envZ ←	sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,529,197	0	A	G	100.0%	293.3 / NA	91	S11P (TCA→CCA)	envZ	sensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/45);  total (46/45)

GTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGA  >  CP009273/3529052‑3529342                                                                                                                                                  |                                                                                                                                                  gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAGAATCGCGAAGTGCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACt                                                                                                                                                >  2:236711/1‑149 (MQ=255) gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACt                                                                                                                                                >  1:547170/1‑149 (MQ=255) gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACt                                                                                                                                                >  1:818323/1‑149 (MQ=255)    atcaACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCg                                                                                                                                             >  1:344080/1‑149 (MQ=255)    atcaACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCg                                                                                                                                             >  2:1227459/1‑149 (MQ=255)     tcaACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGt                                                                                                                                            >  1:587911/1‑149 (MQ=255)     tcaACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAAAGTACGGGCAAATGGACTTCGt                                                                                                                                            >  2:445061/1‑149 (MQ=255)        aCATCCGCACCTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGc                                                                                                                                         <  2:992740/149‑1 (MQ=255)        aCATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGc                                                                                                                                         <  1:1186221/149‑1 (MQ=255)             cGCACTTCGTCACCGAGGAATTGATCAAACTGCTGGAGGCTCGGCAAAATCCTGAAGTTCCGCACCACCAGTTAAGTCGTCACCAGCCCCGCGAACAGCAAGGTGACGATGAGCAATAACGTCCGGGCAAATGGACTTCGTGGCGAGaa                                                                                                                                    <  2:372016/149‑1 (MQ=255)             cGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGaa                                                                                                                                    <  1:1122643/149‑1 (MQ=255)              gCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTAAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAg                                                                                                                                   >  1:447893/1‑149 (MQ=255)                aCTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAgcg                                                                                                                                 <  1:293121/149‑1 (MQ=255)                    cggaCGCGAGGACTTTATTAGACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAAt                                                                                                                             <  1:137730/146‑1 (MQ=255)                    cGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAAt                                                                                                                             <  1:455631/149‑1 (MQ=255)                      tACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACCTCGGGGCCagcagccccaccg                                                                                                                           >  2:1288372/1‑142 (MQ=255)                         gcgAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCt                                                                                                                        >  1:132653/1‑149 (MQ=255)                         gcgAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAACCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCt                                                                                                                        <  2:153022/149‑1 (MQ=255)                          cgAGGCCTTTATGACCTTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCgagataattttgcttttt                                                                                                                       <  2:1215164/149‑15 (MQ=255)                          cgAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTc                                                                                                                       <  2:1280629/149‑1 (MQ=255)                          cgAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTc                                                                                                                       <  1:69326/149‑1 (MQ=255)                              gtcTTTATTAACGTGCTGGCGGCTCGGCAAATTCGCGAAGTTCAGCACCACCACATCAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATTGACCTCGTGGCGAGAAGCGCAATCGCCTCATGc                                                                                                                   <  2:742928/147‑1 (MQ=255)                                gttgatagggcggctgGGGGCTCGGCAAAATCGCGAAGTTCAGGAACGCCAGATAGGGCGTCACCAGGCTGGGGAACAGCAAGGTGGCGATGAGCAATACCGTACGGGCAAATGGGCTTCGTGGCGAGAAGCGCAATCGCCTCATGCtt                                                                                                                 <  2:1236/137‑1 (MQ=255)                                 tttATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGGTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGAGAGAAGCGCAATCGCCTCATGCttt                                                                                                                >  1:815359/1‑149 (MQ=255)                                 tttATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGCGACGATGAGCAATAGCGTACGGGCAAAGGGACTTCGTGGCGAGGAGCGCAATCGCCTCCTGCttt                                                                                                                >  2:442864/1‑149 (MQ=255)                                  ttattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTa                                                                                                               <  1:377839/149‑1 (MQ=255)                                   tattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTag                                                                                                              <  1:1172200/149‑1 (MQ=255)                                     ttaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTagag                                                                                                            <  1:1043723/149‑1 (MQ=255)                                       aaaCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGCAGCGCAATCGCCCCATGCTTTAGAGcc                                                                                                          >  1:256368/1‑149 (MQ=255)                                        aacgcccggAGGCTCGGCAAAATCGCGAAGTTCAGCACGCCCAGATAAGTCGTCACCAGGCCGGCGAACAGCAAGGTGACGAGGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCg                                                                                                         <  2:697070/142‑1 (MQ=255)                                        aaCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCg                                                                                                         >  2:622689/1‑149 (MQ=255)                                              tgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGt                                                                                                   >  1:988331/1‑149 (MQ=255)                                              tgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAACGACGTCCggc                                                                                                   >  2:582088/1‑148 (MQ=255)                                                       ggCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACg                                                                                          >  1:756651/1‑149 (MQ=255)                                                        gCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGt                                                                                         >  2:150412/1‑149 (MQ=255)                                                        gCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGAAGt                                                                                         >  2:140538/1‑149 (MQ=255)                                                         cAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTa                                                                                        >  2:791473/1‑149 (MQ=255)                                                          aaaaTCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAg                                                                                       <  2:425302/149‑1 (MQ=255)                                                           aaaTCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGc                                                                                      <  2:474000/149‑1 (MQ=255)                                                           aaaTCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCACATGCTTTAGAGCCGTCCGGTACAAAGACGTAGc                                                                                      >  1:569717/1‑149 (MQ=255)                                                            aaTCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcc                                                                                     <  1:221079/149‑1 (MQ=255)                                                              tCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGccca                                                                                   <  1:1108124/149‑1 (MQ=255)                                                                gcgAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccaga                                                                                 <  1:570420/149‑1 (MQ=255)                                                                  gAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCACTAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCGTGCTTTTGGACCGTCCGGTACAAAGACGTGGcccggacc                                                                               >  1:1023098/1‑149 (MQ=255)                                                                   aaGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccc                                                                              >  1:722247/1‑149 (MQ=255)                                                                      ttCAGCACCACCAGATAAGGCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATGACCTCATGCTTTAGAGCCGTCCGGTCCAAAGGCGTGGcccagacccaag                                                                           >  1:504402/1‑149 (MQ=255)                                                                        cAGCACCACCAGATAAGTCGTCACCTTGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTAACAAGACGTAGCCCTGA‑CCCAGACg                                                                        >  1:630825/1‑149 (MQ=255)                                                                           caccacCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGt                                                                      >  2:343944/1‑149 (MQ=255)                                                                               accaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCACACCCCAGACGGTCTGa                                                                  <  2:818323/149‑1 (MQ=255)                                                                               accaGATAAGTCGTCACCAGGCTGCTGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGa                                                                  >  1:671224/1‑149 (MQ=255)                                                                               accaGATAAGCCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCTTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGa                                                                  <  2:850051/149‑1 (MQ=255)                                                                                ccaGGTAGGCCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGaa                                                                 <  2:1294436/149‑1 (MQ=255)                                                                                ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATCACGTACGGGGAAATGGACTTCGTGGCGAGACGCGCAATCGCCTCATGGTTTAGAGCCGTCCGGTACACAGACGCAGCCCAGACCCCAGCCGGCCTGaa                                                                 >  2:504985/1‑149 (MQ=255)                                                                                ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGaa                                                                 >  2:180729/1‑149 (MQ=255)                                                                                ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAAAGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGCCGTAGCCCAGACCCCACACGGCCTGaa                                                                 >  2:51792/1‑149 (MQ=255)                                                                                    aTAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACCAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTa                                                             >  1:901334/1‑149 (MQ=255)                                                                                          cgtcACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCTTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCgg                                                       <  1:582088/149‑1 (MQ=255)                                                                                            tcACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGCCGGTCTGAATATAAGGCCGAt                                                     >  2:101245/1‑149 (MQ=255)                                                                                                   gCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTgg                                              <  1:150412/149‑1 (MQ=255)                                                                                                   gCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTgg                                              <  1:533326/149‑1 (MQ=255)                                                                                                   gCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTgg                                              >  2:63657/1‑149 (MQ=255)                                                                                                   gCTGGCGAACAGCAAGGCGACGACGAGCAATAACGTCCGGGCAAATGGACTTCGGGGCCAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGCCCCCAGACGGTCTGAATGTAACGCGGATGCGCTgg                                              <  2:1003477/149‑1 (MQ=255)                                                                                                    cTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCGGAATGTAAAGCGGATGCGCTGGa                                             >  2:771365/1‑149 (MQ=255)                                                                                                    cTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCACATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGa                                             <  2:569717/149‑1 (MQ=255)                                                                                                     tGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGCAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAt                                            >  1:843956/1‑149 (MQ=255)                                                                                                     tGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAt                                            <  1:969669/149‑1 (MQ=255)                                                                                                       gCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCAAGACGGTCTGAATGTGACGCGGATGCGCAGGAtct                                          >  1:26901/1‑149 (MQ=255)                                                                                                           aCAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATcttctt                                      >  1:884343/1‑149 (MQ=255)                                                                                                              gCAAGGTGACGATGAGCAATAACGTACGGGCAACTGGACATCCACGACAAAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAATACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTcca                                   <  2:281605/149‑1 (MQ=255)                                                                                                              gCAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAGCGCGGATGCGCTGGATCTTCTTcca                                   <  1:791473/149‑1 (MQ=255)                                                                                                               cAAGGTGACGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTccac                                  <  1:79784/149‑1 (MQ=255)                                                                                                                      aCGATGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTCGATAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATgcgg                           <  1:311566/149‑1 (MQ=255)                                                                                                                       ggggggggaagaaCGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATgcggc                          <  1:1288372/138‑1 (MQ=255)                                                                                                                         agtagaaaTAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGgcgc                        <  2:72758/143‑1 (MQ=255)                                                                                                                          tGAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTAGAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCa                       <  1:382809/149‑1 (MQ=255)                                                                                                                           gAGCAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAg                      >  1:220865/1‑149 (MQ=255)                                                                                                                              cAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcg                   >  1:1259868/1‑149 (MQ=255)                                                                                                                              cAATAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcg                   >  2:1301444/1‑149 (MQ=255)                                                                                                                               aaTAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCGATCGCCTCACGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcgc                  >  1:84777/1‑149 (MQ=255)                                                                                                                                 tAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGa                >  1:1063226/1‑149 (MQ=255)                                                                                                                                 tAACGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGa                <  2:1079207/149‑1 (MQ=255)                                                                                                                                  aaCGTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGaa               <  2:587911/149‑1 (MQ=255)                                                                                                                                    cGTACGGGCAAATGGACTTCGTGGTGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAAt             <  1:445061/149‑1 (MQ=255)                                                                                                                                     gTACGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATc            <  2:344080/149‑1 (MQ=255)                                                                                                                                       tcGGGCAAAGGGACTGCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTg          <  1:427206/148‑1 (MQ=255)                                                                                                                                        cGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGc         <  2:447893/149‑1 (MQ=255)                                                                                                                                        cGGGCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGc         <  1:1058441/149‑1 (MQ=255)                                                                                                                                         gggCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGTTGCGCTGGATCTTGTTCCATCATGCGGCGAAGGCGTGAAATCTGCa        >  1:352219/1‑149 (MQ=255)                                                                                                                                           gCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACg      >  1:1054843/1‑149 (MQ=255)                                                                                                                                           gCAAATGGACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACg      >  2:850249/1‑149 (MQ=255)                                                                                                                                               aTGGACTTCGCGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCGGTATTTATTGCGGAGTCGCGGGGTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCCCGTCGa  >  1:385856/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGA  >  CP009273/3529052‑3529342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑