New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 3128417 =57 (1.200)2 (0.050) 3/86 NT 4.8% coding (455/1173 nt) yghO predicted DNA‑binding transcriptional regulator
?W3110S.gb 3128445 = 31 (0.780)coding (427/1173 nt) yghO predicted DNA‑binding transcriptional regulator
Rejected: Frequency below/above cutoff threshold.

GTCACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGGAAAGCGGATTACTGATTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3128488‑3128417
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgattgaTATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCA  >  W3110S.gb/3128445‑3128512
                                                                                                                                            
GTCACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGGAAGCGGATTACTGATTGA                                                                      <  1:3242305/71‑1
 TCACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGTAATCCGCTTTCCTGATTGA                                                                      <  1:3459102/71‑1
 TCACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGGAAAGCGGATTACTGATTGA                                                                      <  1:1896209/71‑1
  CACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGGAAAGCGGATTACTGATTG                                                                       >  1:3963115/1‑69
   ACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGGAAAGCGGATTACTGATTG                                                                       >  1:3822871/1‑68
          GCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGTAATCCGCTTTCCTGATTGA                                                                      <  1:4359743/62‑1
                                TCAGCCTGAATATCAATCAGGAAAGCGGATTACTGA                                                                          >  1:1464225/1‑36
                                                TCAGGAAAGCGGATTACTGATTGATATTCAGGCTGAAAGTAC                                                    >  1:4104100/1‑42
                                                 CAGGAAAGCGGATTACTGATTGATATTCAGGCTGAAAG                                                       >  1:2848131/1‑38
                                                                CTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACACTTCCGCTG       >  1:22052/1‑71
                                                                CTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCA                      <  1:2853628/56‑1
                                                                CTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTA                                         >  1:4264873/1‑37
                                                                  GATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCAC                                  >  1:2481106/1‑42
                                                                  GATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCAC                                  >  1:1432773/1‑42
                                                                  GATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCA                                   >  1:2601492/1‑41
                                                                    TTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGC    >  1:431747/1‑70
                                                                    TTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCG     >  1:2826415/1‑69
                                                                    TTGATATTCAGGCTGAAAGGACAGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGC    >  1:2420882/1‑70
                                                                      GATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCA  <  1:2024859/70‑1
                                                                      GATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCA  >  1:167570/1‑70
                                                                      GATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGC               >  1:1823015/1‑57
                                                                                                                                            
GTCACAAGGTGCAAGTAAGATCAGCGGTCCTTTCAGCCTGAATATCAATCAGGAAAGCGGATTACTGATTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3128488‑3128417
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgattgaTATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCA  >  W3110S.gb/3128445‑3128512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.