Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,233,804 1557 bp→GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT coding (47‑1603/1650 nt) pgi → glucosephosphate isomerase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4233804 4235360 1557 96 [0] [0] 77 pgi glucosephosphate isomerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 42338030 (0.000)57 (0.800)
+GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT
38/330 0.5 100% coding (46/1650 nt) pgi glucosephosphate isomerase
?NC_000913 4235361 = 0 (0.000)coding (1604/1650 nt) pgi glucosephosphate isomerase

CGCGAAGTGGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAT  >  NC_000913/4235066‑4235659
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
tttttcatccactcctggcggtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctnaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGa                                                                                                                                                                                                                                                                                                       >  1:998810‑M2/296‑301 (MQ=255)
      atccactcctggcggtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATg                                                                                                                                                                                                                                                                                                 >  1:830171‑M2/290‑301 (MQ=255)
         cacttctcgcggtaagcagtgcatctaataaatgcttcacagcgctaaggattaaccctcaacatgccgctagcggaactaaaaccagcacatttttctgttactggcgctacaatctgccaaagtcacaattctcaaaatcagaagagtattgctcatgaaaaacagcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttccaagttcctatacagtctagagaataggaacttcgaacttcaggtcgacggatccccggaatGCTCGACCAATGGTc                                                                                                                                                                                                                                                                                              <  1:699178‑M2/15‑1 (MQ=255)
                  cggtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAact                                                                                                                                                                                                                                                                                     <  1:114299‑M2/24‑2 (MQ=255)
                   ggtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCg                                                                                                                                                                                                                                                                                    >  1:517905‑M2/277‑301 (MQ=255)
                                      aaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcttggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTATGATTAACCGCTATAAAGCGTGGCGCGGt                                                                                                                                                                                                                                                                 >  1:899482‑M2/258‑301 (MQ=255)
                                        atgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCgg                                                                                                                                                                                                                                                                  >  1:788351‑M2/256‑298 (MQ=255)
                                         tgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTc                                                                                                                                                                                                                                                                                              <  1:50168‑M2/15‑1 (MQ=255)
                                              cactgcgctaagggtttacactcaacattacgctaacgtcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagatgagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGGCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatcatc                                                                                                                                                                                                                                                         <  1:611822‑M2/52‑1 (MQ=255)
                                                  gcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCAtcgtc                                                                                                                                                                                                                                                      >  1:229692‑M2/246‑300 (MQ=255)
                                                         gggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCtat                                                                                                                                                                                                                                                                                <  1:696960‑M2/29‑1 (MQ=255)
                                                         gggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTa                                                                                                                                                                                                                                              >  1:361996‑M2/239‑301 (MQ=255)
                                                          ggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAg                                                                                                                                                                                                                                             >  1:561563‑M2/238‑301 (MQ=255)
                                                           gtttaccatcaacattaagctaacggcacgaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                               <  1:773753‑M2/62‑1 (MQ=255)
                                                             ttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGcc                                                                                                                                                                                                                                          <  1:104438‑M2/67‑1 (MQ=255)
                                                             ttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgatacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGcc                                                                                                                                                                                                                                          <  1:224368‑M2/67‑1 (MQ=255)
                                                                      acattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTACTCATCGTCGATATGTAGGACGGATAaggg                                                                                                                                                                                                                                 >  1:250274‑M2/226‑300 (MQ=255)
                                                                      acattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAaggg                                                                                                                                                                                                                                 >  1:404464‑M2/226‑300 (MQ=255)
                                                                       cattacgctaacggcactaaaaccatcacatttttctgtgactcgcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                                                                                                                                                                                <  1:822449‑M2/77‑1 (MQ=255)
                                                                            cgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                                                                                                                                                                                           <  1:710340‑M2/82‑1 (MQ=255)
                                                                                    gcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctattctttctagagaataggaacttcgaactgacggacgacggatccccggaatGCTCgan                                                                                                                                                                                                                                                                                                      >  2:717590‑M2/212‑217 (MQ=255)
                                                                                    gcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                               >  1:418131‑M2/212‑273 (MQ=255)
                                                                                                             tgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATaa                                                                                                                                                                                                                                                                              <  2:79602‑M2/31‑1 (MQ=255)
                                                                                                             ggactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                                                                                                                                           <  1:400427‑M2/114‑1 (MQ=255)
                                                                                                                        ncaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCgg                                                                                                                                                                                                                                                                  <  2:788331‑M2/43‑1 (MQ=255)
                                                                                                                           atcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  1:569572‑M2/129‑1 (MQ=255)
                                                                                                                           atcttccaaagtcacaattctcaaaatcagaagagtatggctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  1:61807‑M2/129‑1 (MQ=255)
                                                                                                                                      ccacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggaactacggtgtaggctggagctgcttcgaagttcatatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                                                                                                                                 <  1:229463‑M2/140‑1 (MQ=255)
                                                                                                                                           attctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                               <  2:517897‑M2/62‑1 (MQ=255)
                                                                                                                                           attctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                               <  2:418124‑M2/62‑1 (MQ=255)
                                                                                                                                               tcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatacttgctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGc                                                                                                                                                                                                                                           <  2:5356‑M2/66‑1 (MQ=255)
                                                                                                                                                        gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAagn                                                                                                                                                                                                                                  >  2:982926‑M2/144‑217 (MQ=255)
                                                                                                                                                         aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                                                                                                                                 <  2:105491‑M2/76‑1 (MQ=255)
                                                                                                                                                         aagagtattgctaatgaaaaacatcaagccaacgcagaccactgcctggcaggcactacggtgtaggctggggctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                <  1:349520‑M2/157‑1 (MQ=255)
                                                                                                                                                            agtattgctaatgaaaagcatcaatccaacgcagaccgctgcctggcaggcgctacggtgtaggctggagctgcttcgaagttcctatgctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                                                                                                                                                                                <  2:335115‑M2/77‑1 (MQ=255)
                                                                                                                                                            agtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttccgatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                                                                                                                                 <  2:893522‑M2/76‑1 (MQ=255)
                                                                                                                                                            agtattgcgaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcacgacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagcaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTNATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                                                                                                                                            <  1:986408‑M2/161‑1 (MQ=255)
                                                                                                                                                                   ctaacgaaaaacatcaatccaactcagaccgctgccgggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctatagaataggaacttcgaactgcaggtcgacggatccccgggatGCTCGACCAATGGTCTGATTAACCGCTATAAGGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                    <  1:390382‑M2/169‑1 (MQ=255)
                                                                                                                                                                   cgaaggaaaaccagcaatccaacgcagacagctgcagggaaggcaatacggtgtaggctggagctgcttcgaagttcctatactgtctagagaataggaactgcgaactggaggtcgaaggatccccggaatGCTCGACCAATTGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                    <  1:179741‑M2/169‑1 (MQ=255)
                                                                                                                                                                          aacacatcaatccaacgcatgccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagtgactagactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCACTGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                                                                                                                             <  1:604666‑M2/176‑1 (MQ=255)
                                                                                                                                                                            aacgacaatcctacgcagcccgctgcctggcaggcactacggtgtaggcgggagcttcttcgaagttcctatactttcgagagaataggtactgcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                           <  1:613636‑M2/178‑1 (MQ=255)
                                                                                                                                                                            aacatcaatccaacgcagaccgctgccgggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                           <  1:242960‑M2/178‑1 (MQ=255)
                                                                                                                                                                            aacatcaagccaccgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                           <  1:648996‑M2/178‑1 (MQ=255)
                                                                                                                                                                            aaaatcaagcccacgcagacagctgcttgccaggtacgacggtgtaggctggagctgcgtcaaagttcctatactttctagagaatagtaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCTTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                           <  1:858136‑M2/178‑1 (MQ=255)
                                                                                                                                                                             acatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                                                                                                                             >  2:412848‑M2/123‑218 (MQ=255)
                                                                                                                                                                              catcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                                         <  1:920387‑M2/180‑1 (MQ=255)
                                                                                                                                                                              catcaatccaacgcagaccgctgcctggcaggaattacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                                         <  1:525666‑M2/180‑1 (MQ=255)
                                                                                                                                                                                 caatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                                                                                                                                         <  2:292333‑M2/100‑1 (MQ=255)
                                                                                                                                                                                   atccacagcagaccgctgcctggcaggcaatacggtgtaggctggagctgcttcgaagttactatactttatagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAc                                                                                                                    <  1:746747‑M2/185‑1 (MQ=255)
                                                                                                                                                                                   atccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGNGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAc                                                                                                                    <  1:982951‑M2/185‑1 (MQ=255)
                                                                                                                                                                                      caacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat                                                                                                                 <  1:265305‑M2/188‑1 (MQ=255)
                                                                                                                                                                                              accgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgatt                                                                                                            <  1:960251‑M2/193‑1 (MQ=255)
                                                                                                                                                                                                            ggaacgacggtgaacgctggagctgattcgaagtgcctatactttatagagaataggaacttcgaactgcaggtcgacggatccccggaatGCACGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAAc                                                                                            <  1:213838‑M2/209‑1 (MQ=255)
                                                                                                                                                                                                             tgactacgttgtaggctggagctgcttcgaagtttctatactttctatagaataggaacttcgaaccgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAAc                                                                                            <  1:1013159‑M2/209‑1 (MQ=255)
                                                                                                                                                                                                              nactagggttagcgctggcgctgcttcgaagttcctagactttctagagaataggaatttcgaactgcaggtcgacggatcccgggaatGCTAGACCAAGGGTCTGTTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  2:762645‑M2/129‑1 (MQ=255)
                                                                                                                                                                                                              nactacggtgtatgctggagcttcttcgaagttcctatgcgttctagagaataggaacttctaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTAGAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  2:952469‑M2/129‑1 (MQ=255)
                                                                                                                                                                                                              cactaaggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  2:69223‑M2/129‑1 (MQ=255)
                                                                                                                                                                                                                  acggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCg                                                                                     >  1:909615‑M2/86‑301 (MQ=255)
                                                                                                                                                                                                                        taggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGTTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                                                                                                                                  <  2:361989‑M2/139‑1 (MQ=255)
                                                                                                                                                                                                                        taggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                                                                                                                                  <  2:404457‑M2/139‑1 (MQ=255)
                                                                                                                                                                                                                           nctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                                                                                                                               <  2:998775‑M2/142‑1 (MQ=255)
                                                                                                                                                                                                                                      ttcgaagttcctatactttctagagaataggaacttcgaacggcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCatttttg                                                                 <  1:135447‑M2/236‑2 (MQ=255)
                                                                                                                                                                                                                                          aagttcctatgctttctagagaataggaacttctaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTAGAAGGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTC‑‑‑TCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                              <  2:406612‑M2/156‑1 (MQ=255)
                                                                                                                                                                                                                                             ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                              <  2:246477‑M2/159‑1 (MQ=255)
                                                                                                                                                                                                                                             ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCCATTAATACATAGCACGATTGATTACATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGa                                                          >  1:217464‑M2/59‑301 (MQ=255)
                                                                                                                                                                                                                                                              gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTAGGCCTTTTTTCATTAGATAAATCCCCTTGt                                             >  1:481313‑M2/42‑297 (MQ=255)
                                                                                                                                                                                                                                                               aataggaactgcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTAGAAGGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAACCGAGGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                           <  2:157431‑M2/178‑1 (MQ=255)
                                                                                                                                                                                                                                                                  atggactgcaggccgcagggcgacggatccccggaacGGTAGACCAATGGTATGATTAACCGCTATAAAGCGTGGCGCGGGTAATAATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTTTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAAt                                      <  1:601404‑M2/263‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                                         <  2:706350‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tcgaactgcaggtcgacgtatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATAGGTAGGCCGGATAAGGCGTTCACGCCGCAGCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat                                                                                                                 <  2:49757‑M2/188‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ncgaactgcaggtcgacggatccccggaacGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATCAATACATAGCACgat                                                                                                                 <  2:1012134‑M2/188‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cagctcgacggatccccggattGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATAGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgatt                                                                                                            <  2:561555‑M2/193‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    gtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAAt                                                                                                        <  2:589629‑M2/197‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              cccgtgaatGATCGACCAATGGGCTGATGAACAGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGAAACCGATGCCTGATGCGACGCGCTCGCGTCTTAGCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAAc                                                                                            <  2:54249‑M2/209‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                ccggaagGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACaa                                                                                          <  2:229687‑M2/211‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     atGCTCGACCAATGGTCTGATGAACCGCTATAAAGCGGGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAt  <  1:183105‑M2/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     atGCTCGACCAATGGACTGATTAACCGCGATAAAGCGTGGCGCGGTTAATAATCGGAGATATGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAt  <  1:717603‑M2/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
CGCGAAGTGGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAT  >  NC_000913/4235066‑4235659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: