Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,929,980 (G)7→8 intergenic (‑84/‑145) RPPX_17505 ← / → RPPX_17510 ATPase AAA/type IV secretion protein Rhs

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,929,9731.G88.5% 494.6 / 65.7 156intergenic (‑77/‑152)RPPX_17505/RPPX_17510ATPase AAA/type IV secretion protein Rhs
Reads supporting (aligned to +/- strand):  ref base . (6/12);  new base G (103/35);  total (109/47)
Fisher's exact test for biased strand distribution p-value = 7.37e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

ACCAGTTTCTCCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTCGGACAAGCAGGA  >  CP009974/3929823‑3930118
                                                                                                                                                        |                                                                                                                                                 
aCCAGTTTCTCCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATcc                                                                                                                                                    <  1:1273115/151‑1 (MQ=255)
  cAGTTTCTCCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCgg                                                                                                                                                 <  2:1631442/151‑1 (MQ=255)
  cAGTTTCTCCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCgg                                                                                                                                                 >  2:591223/1‑151 (MQ=255)
   ggTTTCTCCTGGCCTAGGATCTGGGTAGAAAGAAACGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCggg                                                                                                                                                <  2:2002501/150‑1 (MQ=255)
     tttCTCCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCggggg                                                                                                                                              <  1:1246308/151‑1 (MQ=255)
       tctcCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCggggggg                                                                                                                                            <  1:1847699/151‑1 (MQ=255)
       tccccTGGCCTAGGATCGGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCggggggg                                                                                                                                            <  1:2293705/148‑1 (MQ=255)
        ctcCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCgggggggg                                                                                                                                           >  1:2032116/1‑150 (MQ=255)
           cTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCggggggggcga                                                                                                                                        >  1:419301/1‑147 (MQ=255)
            gggCCGAGGAGCTGGGGAGAAAGGAAAGGGCGCGGCGCGGTCAGCGCTGCACGGTGTTCCATGGGCGACGGTTCCTGGGAGGGGGCCGGAGGCGGCGTGGGGCAGCGGCCTTGGCAAGGGGGGATACTTTAATCTATCCggggggggcgac                                                                                                                                       <  2:3592403/150‑6 (MQ=255)
             ggCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGcgacg                                                                                                                                       >  2:2101024/1‑151 (MQ=255)
                  aGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCaa                                                                                                                                  >  2:270420/1‑151 (MQ=255)
                   ggATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAAt                                                                                                                                 >  2:2948733/1‑151 (MQ=255)
                   ggATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAAt                                                                                                                                 >  1:1406856/1‑151 (MQ=255)
                    gATCTGGGTAGAATGTTTAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATg                                                                                                                                >  2:1237383/1‑151 (MQ=255)
                      tCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATggg                                                                                                                              >  1:3444233/1‑150 (MQ=255)
                         ggggAGCAAGAAGTGGGCGGAGCGCGATGGGGGCGTCACGGTGTTCCAGCGGCGACGGGTCGTGGTAGGGGGCCTGAGGCGGCGGGAGGCAGCAGCCTTGGGAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATggcggc                                                                                                                           <  2:3064987/151‑1 (MQ=255)
                           ggggaaagaagAGTGCGCAGGGGGATCGGGGCTTCAGGGTGTTCCATCGGGGGGGGTTCGTGGTAGGGGGCCGGGGGCGGCGTGAGGCGGCAGCCTTGGCAGGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGcggcgg                                                                                                                         <  2:2217684/148‑1 (MQ=255)
                            tAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTATTCTATCCGGGGGGGGCGACGACCAATGGCGGCggg                                                                                                                        >  2:722410/1‑151 (MQ=255)
                            gagaaagagAAGGGGGCGGCGCGAGCAGCGCTTCACGGGGTGCCAGCGGGGAGGGTGCCGGGTAGGGGGCCGGAGGCGGCGTGAGGCAGAGGGCTTGGAAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCggg                                                                                                                        <  2:4341575/150‑1 (MQ=255)
                             agaaagaaAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGt                                                                                                                       >  2:4728061/1‑151 (MQ=255)
                                aagaaaAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTaaa                                                                                                                    >  2:2339491/1‑151 (MQ=255)
                                 agaaaAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAAt                                                                                                                   >  1:1156605/1‑151 (MQ=255)
                                 agaaaAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTCGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTagcc                                                                                                                   >  2:4076673/1‑148 (MQ=255)
                                      ggTGCGCGGGGCGATCAGCGCTTCACGGTGTCCCTCGGGGGCCGGTTCCTGGGAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATc                                                                                                              <  2:4705467/150‑1 (MQ=255)
                                      aGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATc                                                                                                              >  2:1770443/1‑151 (MQ=255)
                                      aGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATc                                                                                                              >  1:4991683/1‑151 (MQ=255)
                                      aGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATc                                                                                                              >  1:151801/1‑151 (MQ=255)
                                       gTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCg                                                                                                             >  1:476597/1‑151 (MQ=255)
                                         gcgcAGCGCGAGCAGCGCGTCAGGGTGTTCCAGCGGGGGGGGTTCCGGGTAGGGGGCGTGAGGCGGCGGGAGGGAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGtt                                                                                                           <  1:2971398/151‑1 (MQ=255)
                                          cgcAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTg                                                                                                          >  1:432207/1‑151 (MQ=255)
                                             aGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAg                                                                                                       >  2:1416790/1‑151 (MQ=255)
                                              gggggATCAGCGCTTCACGGTGGTCCATCGGCGGCGGTTCCGGGTAGGGGGCTGGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGa                                                                                                      <  2:5061531/147‑1 (MQ=255)
                                               ggcgATCGGGGCTGCACGGTGTTCCGGCGGCGACGGTTCCTGGGAGGGGGCCTGGGGCGGGGTGGGGCAGCAGCCTTGGCAGGGGGGGATACTTTAATCTATCGGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGAc                                                                                                     <  1:661617/150‑1 (MQ=255)
                                               cgcgATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATGGTTGCAGAc                                                                                                     >  2:4110027/1‑151 (MQ=255)
                                               cccgctCACCGCTCCGCGGTGTTCCATGGGCGACGGTTCCTGGTTGGGGGCCGGTGGCGGCGTGGGGCGGCAGCCTTGGCAAGGGGGGATCCTTTAATCAATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGAc                                                                                                     <  1:1313671/146‑1 (MQ=255)
                                                gcgATCAGCGCTTCTCGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTCTCCGGGTGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACgg                                                                                                   >  2:3623115/1‑151 (MQ=255)
                                                gcgATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACg                                                                                                    >  2:242387/1‑151 (MQ=255)
                                                   aTCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGc                                                                                                 >  1:608749/1‑151 (MQ=255)
                                                     cAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCt                                                                                               >  1:4389382/1‑151 (MQ=255)
                                                      aGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTg                                                                                              >  1:4663454/1‑151 (MQ=255)
                                                      aGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTg                                                                                              >  1:4663305/1‑151 (MQ=255)
                                                      aGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGACCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGCCCAATGGCGGCGCGCAAATTTATCGTTGCAGACGGGCCTg                                                                                              >  2:5222815/1‑151 (MQ=255)
                                                         gcTTCACGGTGTTCCATCGGCGACGGTTCCCGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATAGTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTACATTTATCGTTGCAGACGGGCCTGCgg                                                                                           >  2:3122228/1‑151 (MQ=255)
                                                          gggcACGGTGTTCCATGGGCGACGGTTCTTGGTAGGGGGCCTGAGGCGGCGGGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCggg                                                                                          <  2:1840792/148‑1 (MQ=255)
                                                          cTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCggg                                                                                          >  2:1413059/1‑151 (MQ=255)
                                                               gggTGTTCCAGCGGCGGCGGTTCCTGGGAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAg                                                                                     <  2:1880172/150‑1 (MQ=255)
                                                               cGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGTGGTGTGAGGCAGCTGCCTTGGCAAGGGGGGTTACTTTAATCTATCCGGGGGGGGTGACGACCAATGGCGGCGGGTAAAGTTATCGTTGCAGACGGGCCTGCGGGCATAg                                                                                     >  2:1316610/1‑151 (MQ=255)
                                                               cGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGa                                                                                    >  2:120967/1‑151 (MQ=255)
                                                                   gtTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAAt                                                                                 >  1:4250415/1‑151 (MQ=255)
                                                                      cgaTCGGCGAGGGTTCCTGGAAGGGGGCCGGAGGCGGGGGGGGGCAGCAGCAGTGGAAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTc                                                                              <  1:285333/149‑1 (MQ=255)
                                                                       gggcGGCGACGGTGGCGGGTGGGGGGTCGGAGGGGGCGGGAGGCAGCAGCCTTGGCAGGGGGGGATACTTTGATCTATCGGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTcc                                                                             <  2:2787689/148‑1 (MQ=255)
                                                                        aTCGGGGAGGGTTGTTGGTAGGGGGCCGGAGGGGGGGGGACGCGGCAGCGTTGGCAGGGGGGGATACTTTAATCTATCGGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTccc                                                                            <  1:3642437/151‑1 (MQ=255)
                                                                          gggCGACGGTTGCTGGTAGGGGGTCTGAGGCGGGGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACg                                                                         <  2:3244256/150‑1 (MQ=255)
                                                                          cGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGCCGACCAATGGCGGCGGGGACATTTATCGTTGCAGACGGGCCTGCGGGCCAAGAAATGTccccc                                                                          >  2:2655556/1‑149 (MQ=255)
                                                                           ggCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACg                                                                         >  1:3873342/1‑151 (MQ=255)
                                                                           ggCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACg                                                                         >  1:2936640/1‑151 (MQ=255)
                                                                             cGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCa                                                                       >  2:810343/1‑151 (MQ=255)
                                                                                 ggTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCAtggc                                                                   >  2:3377211/1‑151 (MQ=255)
                                                                                 ggTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCAtggc                                                                   >  2:2387198/1‑151 (MQ=255)
                                                                                 ggTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCAtggct                                                                  >  2:4717373/1‑151 (MQ=255)
                                                                                   taccgggTAGGGGGCGTGAGGCGGCGTGAGGCAGCAGCCATGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCAtggctg                                                                 <  2:409832/146‑1 (MQ=255)
                                                                                     ccTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAACTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCAtggctggc                                                               >  2:405721/1‑151 (MQ=255)
                                                                                     ccTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGcc                                                              >  1:4422807/1‑151 (MQ=255)
                                                                                      gtGGGAGGGGGCCTGGGGCGGCGCGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATGTATCGGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGcc                                                              <  2:4981611/150‑1 (MQ=255)
                                                                                       tGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCt                                                             >  1:3032891/1‑151 (MQ=255)
                                                                                       ggggaGGGGGCGGGGGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTAGCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCt                                                             <  2:1268778/147‑1 (MQ=255)
                                                                                          tAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCTAAGAAATGTCCCACGCCTGGCTGGCCTTTg                                                          >  2:2329953/1‑151 (MQ=255)
                                                                                          tAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACCCTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCCTTGCAGACGGGCCTGCGGGCAAGGGAAAGGTCCACCCCTGGCTGGCCttcg                                                          >  2:963275/1‑149 (MQ=255)
                                                                                           aGGGAGCCTGAGGCGGCGTGAGACAGCATCCTTGACAAGGGGTGATACTTACTCCTACCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGc                                                         <  2:2768036/151‑1 (MQ=255)
                                                                                             ggggCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCt                                                       >  1:3676804/1‑151 (MQ=255)
                                                                                                  cTGAGGCGACGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGACATGTCCCACGCATCGCTGGCCTTTGCCTGACgg                                                  >  2:3119563/1‑151 (MQ=255)
                                                                                                   tGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAACGGCGGCGGGTAAATCTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTCGCCTTTGCCTGATggc                                                 >  2:1929539/1‑150 (MQ=255)
                                                                                                    gAGGCGGCGTGAGGCAGCGGCCTTGGCAAGGGGGGATACTTTGAGCGATCCGGGGGGGGCGACGACCATTGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTc                                                <  2:2021017/151‑1 (MQ=255)
                                                                                                     gggcggcGTGGGGCAGCAGCCTGGGCAAGGGGGGATACTTTAATGTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTcc                                               <  1:117632/150‑1 (MQ=255)
                                                                                                     agcgggcgggcgGCAGCGGCCGTGGCAAGGGGGGATACTTTAGTCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTAGCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTcc                                               <  2:4837836/147‑1 (MQ=255)
                                                                                                         ggcgTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGa                                           >  1:3919397/1‑151 (MQ=255)
                                                                                                           cgaggggcggcAGCGTGGGCACGGGGGGCGATGTTAGTCGATCGGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACg                                         <  1:4885683/146‑1 (MQ=255)
                                                                                                            gTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTGCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACgg                                        >  2:105284/1‑151 (MQ=255)
                                                                                                            gTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACgg                                        >  1:4167465/1‑151 (MQ=255)
                                                                                                              gAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCg                                      >  1:3865381/1‑151 (MQ=255)
                                                                                                               aGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGt                                     <  2:2900222/151‑1 (MQ=255)
                                                                                                                ggCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTg                                    >  2:3971000/1‑151 (MQ=255)
                                                                                                                   ggcagcCTTGGCAAGGGGGGATACTTTAATCTAGCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTg                                 <  1:2686317/150‑1 (MQ=255)
                                                                                                                   agcagcCTGGGAAAGGGGGGAGACTTTAATCTGTCGGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTg                                 <  1:2685333/151‑1 (MQ=255)
                                                                                                                      agcCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGgcc                              >  2:1769055/1‑150 (MQ=255)
                                                                                                                       gcCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGgcgc                             >  2:4209533/1‑151 (MQ=255)
                                                                                                                         tttgctaaGGGGGGATACGTTACGCTCTCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCt                           <  1:357206/145‑1 (MQ=255)
                                                                                                                         cTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCGGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGccc                           >  2:3507843/1‑150 (MQ=255)
                                                                                                                            ggCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCt                        >  1:3193504/1‑151 (MQ=255)
                                                                                                                             gCAAGGGGGGATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCtt                       >  2:4802508/1‑151 (MQ=255)
                                                                                                                                  gggggATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCt                  >  2:1672806/1‑151 (MQ=255)
                                                                                                                                    gggATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCttt                >  1:4395068/1‑151 (MQ=255)
                                                                                                                                     ggATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCtttt               >  1:2652457/1‑151 (MQ=255)
                                                                                                                                      gATACTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTc              <  1:619899/151‑1 (MQ=255)
                                                                                                                                         aCTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTCggg           >  2:398333/1‑150 (MQ=255)
                                                                                                                                         aCTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTCGGa           >  2:4409355/1‑151 (MQ=255)
                                                                                                                                          cTTTAATCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGATTTTCGGAc          >  2:614221/1‑151 (MQ=255)
                                                                                                                                                tCTATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTCGGACAAGCag    >  1:3608082/1‑151 (MQ=255)
                                                                                                                                                  tATCCGGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTCGGACAAGCagga  >  2:3052857/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                 
ACCAGTTTCTCCTGGCCTAGGATCTGGGTAGAAAGAAAAGTGCGCAGCGCGATCAGCGCTTCACGGTGTTCCATCGGCGACGGTTCCTGGTAGGGGGCCTGAGGCGGCGTGAGGCAGCAGCCTTGGCAAGGGGGGATACTTTAATCTATCCGGGGGGGCGACGACCAATGGCGGCGGGTAAATTTATCGTTGCAGACGGGCCTGCGGGCAAAGAAATGTCCCACGCATGGCTGGCCTTTGCCTGATGGTCCGTGACGGCGTGCTGGCGCCTGCTTGTGCTTTTCGGACAAGCAGGA  >  CP009974/3929823‑3930118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: