Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,844,127 C→T intergenic (+345/‑1) RPPX_17120 → / → RPPX_17125 glutamyl‑tRNA synthetase/tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,844,1270CT99.1% 848.0 / ‑5.4 232intergenic (+345/‑1)RPPX_17120/RPPX_17125glutamyl‑tRNA synthetase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (111/118);  total (112/120)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 246 total aligned reads displayed.

GATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCC  >  CP009974/3843979‑3844277
                                                                                                                                                    |                                                                                                                                                      
gATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTgg                                                                                                                                                      >  1:5158636/1‑151 (MQ=255)
gATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTgg                                                                                                                                                      >  1:4349328/1‑151 (MQ=255)
 aTTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTggg                                                                                                                                                     >  2:4302421/1‑151 (MQ=255)
   tGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGc                                                                                                                                                   <  1:4859780/151‑1 (MQ=255)
     ttATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCta                                                                                                                                                 >  1:3443829/1‑151 (MQ=255)
       aTCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCtata                                                                                                                                               >  1:4382256/1‑151 (MQ=255)
       aTCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCtata                                                                                                                                               <  1:736480/151‑1 (MQ=255)
       aTCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCtata                                                                                                                                               >  1:3751653/1‑151 (MQ=255)
       aTCTGTGAAAAAATTTTTGAATATTTTTAGAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCtata                                                                                                                                               >  2:3083346/1‑151 (MQ=255)
        tCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAg                                                                                                                                              >  1:4582853/1‑151 (MQ=255)
         cTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGc                                                                                                                                             <  1:4738569/151‑1 (MQ=255)
         cTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGc                                                                                                                                             >  1:3317772/1‑151 (MQ=255)
         cTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGc                                                                                                                                             <  1:526486/151‑1 (MQ=255)
         cTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGc                                                                                                                                             <  2:4437572/151‑1 (MQ=255)
          tgtgAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCt                                                                                                                                            <  1:543888/151‑1 (MQ=255)
            tgAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCa                                                                                                                                          >  2:3353179/1‑151 (MQ=255)
                aaaaTTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTg                                                                                                                                      >  1:2064822/1‑151 (MQ=255)
                aaaaTTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTg                                                                                                                                      <  2:117976/151‑1 (MQ=255)
                   aTTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGAGGGGCTATAGCTCAGCTGGga                                                                                                                                   >  1:1073631/1‑151 (MQ=255)
                    tttttGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGgag                                                                                                                                  <  2:3887610/151‑1 (MQ=255)
                     ttttGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGgaga                                                                                                                                 <  1:2732715/151‑1 (MQ=255)
                      tttGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGagag                                                                                                                                >  2:5227716/1‑151 (MQ=255)
                      tttGAATATGTTTGAAAATGAGTTTGACAGCCCTGCAATCCGATCTTAATACGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGagag                                                                                                                                <  2:3452345/151‑1 (MQ=255)
                       ttGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAgc                                                                                                                               >  1:4489805/1‑151 (MQ=255)
                        tGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAgcg                                                                                                                              <  2:3373266/151‑1 (MQ=255)
                        tGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAgcg                                                                                                                              >  1:5201006/1‑151 (MQ=255)
                            tatTTTTTTAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACATCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGTCTATAGCTCAGCTGGGAGAGCGCCTg                                                                                                                          >  2:1212110/1‑151 (MQ=255)
                              tttttGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgca                                                                                                                        <  1:1815688/151‑1 (MQ=255)
                              tttttGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgca                                                                                                                        <  2:2715739/151‑1 (MQ=255)
                              tttttGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgca                                                                                                                        >  1:1911556/1‑151 (MQ=255)
                                tttGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatg                                                                                                                      >  1:869734/1‑151 (MQ=255)
                                tttGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatg                                                                                                                      >  1:4028475/1‑151 (MQ=255)
                                   gAAAGTTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggca                                                                                                                   <  2:1282941/151‑1 (MQ=255)
                                    aaaaTTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcat                                                                                                                  <  1:4237783/151‑1 (MQ=255)
                                     aaaTTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcatg                                                                                                                 >  1:515138/1‑151 (MQ=255)
                                      gaTTAGTTACACAGCCCTGCAATCCGATCTACATATGCGCCCGGTCCACAGCGATGAACGGAAACGAAGCGCCAGGCACCTAGCCAGCGATTCAGTTCAAAGCAACATTGCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGc                                                                                                                <  2:1860901/150‑1 (MQ=255)
                                      aaTTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGc                                                                                                                >  2:5117259/1‑151 (MQ=255)
                                      aaTTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGc                                                                                                                >  2:1136603/1‑151 (MQ=255)
                                       aTTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCa                                                                                                               >  1:688519/1‑151 (MQ=255)
                                          aGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagga                                                                                                            >  1:3292640/1‑151 (MQ=255)
                                               gACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagg                                                                                                             >  1:830573/1‑145 (MQ=255)
                                               gACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagg                                                                                                             <  2:830573/145‑1 (MQ=255)
                                               gACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCa                                                                                                       <  2:916838/151‑1 (MQ=255)
                                               gACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCa                                                                                                       <  1:466967/151‑1 (MQ=255)
                                                     ccTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGtt                                                                                                 >  1:4484657/1‑151 (MQ=255)
                                                      cTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                >  1:958085/1‑151 (MQ=255)
                                                      cTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGAGTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGATCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                >  1:1681512/1‑151 (MQ=255)
                                                       tGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCg                                                                                               >  1:4243599/1‑151 (MQ=255)
                                                       tGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCg                                                                                               <  1:4069114/151‑1 (MQ=255)
                                                           aTCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATcc                                                                                           <  1:2501000/151‑1 (MQ=255)
                                                            tCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCg                                                                                                    >  2:2369647/1‑141 (MQ=255)
                                                            tCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATccc                                                                                          >  1:5091948/1‑151 (MQ=255)
                                                            tCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATcac                                                                                          <  2:4582903/151‑3 (MQ=255)
                                                                   ttAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGc                                                                                   <  2:2175951/151‑1 (MQ=255)
                                                                    tAATATGCGCCCCGTCCACAGCGATGAACGAAACCGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGACTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTCGCt                                                                                  >  1:3667655/1‑151 (MQ=255)
                                                                        atGCGCCCCATCTACAGCGATGAACGAAAACGAATTGCAAGGCACCCAGCCAGCGATTCAGTTCAAAGATACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                                              <  2:1607241/151‑1 (MQ=37)
                                                                         tGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacc                                                                             >  2:20729/1‑151 (MQ=255)
                                                                          gcgcCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacca                                                                            <  1:3353179/151‑1 (MQ=255)
                                                                              cccGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAtttc                                                                        <  1:1129534/151‑2 (MQ=255)
                                                                                cgcccACAGCGATGAACAGAAACGAAGAGGCAGGCTCCTTACGAGCGAGGCAGTTCAAAGTTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTg                                                                      <  2:1007516/148‑1 (MQ=21)
                                                                                cGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattcc                                                                      >  1:3420157‑M1/1‑146 (MQ=255)
                                                                                  tCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGcc                                                                    >  2:3784451/1‑151 (MQ=255)
                                                                                   ccACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcc                                                                   >  1:4679088‑M1/1‑143 (MQ=255)
                                                                                       aGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagc                                                               <  1:1463342‑M1/151‑13 (MQ=255)
                                                                                        gCGATGAACGAAAACGGAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagca                                                              <  2:3067388‑M1/151‑14 (MQ=255)
                                                                                              aacgaaaacacaGCGGCGAGGACCCAGTGAGCGAGTCAGGCGAGGGCAAAATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGtt                                                        <  2:4690352/151‑1 (MQ=18)
                                                                                                cgaaaacgatGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagg                                                                                                             <  2:5020166/96‑1 (MQ=255)
                                                                                                cgaaaacgaaGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagg                                                                                                             >  1:5020166/1‑96 (MQ=255)
                                                                                                cgaaaacgaaGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttggcagccaaggc                                                      <  1:1786871‑M1/151‑22 (MQ=255)
                                                                                                  aaaacgaaGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctg                                                    >  1:212280‑M1/1‑128 (MQ=255)
                                                                                                      ggaaGCGGCGGGGGCCGAGCGAGGGATGGAGGTCAGAGCGACAGTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATc                                                <  2:4385939/150‑1 (MQ=21)
                                                                                                      cgaaGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataa                                                <  1:3923889‑M1/151‑28 (MQ=255)
                                                                                                      cgaaGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATc                                                <  2:28471/151‑1 (MQ=255)
                                                                                                          gcgacgagCAGCCAGCCAGGGATGCAGGTCAAAGCAACAATGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCa                                            <  2:4174710/144‑1 (MQ=25)
                                                                                                             ccAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagat                                         <  2:570308‑M1/151‑35 (MQ=255)
                                                                                                              cAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTTCCCGCGGCTCGCGTTAGAGATCGGCagagcg                                       >  2:31220/1‑146 (MQ=25)
                                                                                                                  cACCCAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggt                                    >  1:4445520‑M1/1‑112 (MQ=255)
                                                                                                                    cccAGCCAGCGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccccgtggctcccaagagcgataagcaagatcgggtcc                                  >  1:4575098‑M1/1‑110 (MQ=255)
                                                                                                                       agccagcGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagc                               >  1:3049790‑M1/1‑107 (MQ=255)
                                                                                                                        gcgagcgatTCAGTTCAAGGCTAGGTTGTGGGGCTAGAGGTCAGCGGGGCGCGCGCCTGCGTGGCAGGCAGGGGGTCGGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagca                              <  2:4069608‑M1/148‑46 (MQ=255)
                                                                                                                          cagcGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTcccc                            <  2:515152/151‑1 (MQ=255)
                                                                                                                           agcGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaatgctgataagcaagatcgggtccagcacac                           >  1:2297583‑M1/1‑103 (MQ=255)
                                                                                                                            gcGATTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCtt                          >  1:3975781/1‑151 (MQ=255)
                                                                                                                               aTTCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagcaaaccggt                       <  2:2267648‑M1/151‑53 (MQ=255)
                                                                                                                                 tCAGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagcaaaccggtct                     >  2:3284913‑M1/1‑97 (MQ=255)
                                                                                                                                   aGTTCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccacggccgatccgccagctcgggtccagccaccccgtctag                   >  2:5168683‑M1/1‑95 (MQ=255)
                                                                                                                                      tCAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgctcagcagccaatgctggtacgcaagatcgggtccagcaaaccggtcttgtag                >  2:4538983‑M1/1‑92 (MQ=255)
                                                                                                                                       cAAAGCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagcaaaccggtcttgaagg               <  1:1383296‑M1/151‑61 (MQ=255)
                                                                                                                                           gCTACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagcaaaccggtcttgaaggtaag           <  1:2584789‑M1/151‑65 (MQ=255)
                                                                                                                                             tttgtttcGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGGTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGa         >  1:3493648/8‑151 (MQ=18)
                                                                                                                                             tACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcat                                                                                                                  >  1:1574105/1‑46 (MQ=255)
                                                                                                                                             tACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAatttcgcttagcagccaaggctgatacgccagatcgggtccagcacaccggtcttgaaggtaagca         >  2:4356934‑M1/1‑85 (MQ=255)
                                                                                                                                             tACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgctcagcagccaaggctgatacgcaagatcgggtccagcaacccggtcttgaaggtaagaa         >  1:235515‑M1/1‑85 (MQ=255)
                                                                                                                                             tACATTGCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcat                                                                                                                  <  2:1574105/46‑1 (MQ=255)
                                                                                                                                              ttgtttcGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGac        >  2:3971945/7‑151 (MQ=18)
                                                                                                                                                aTTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                >  1:3203601/1‑61 (MQ=255)
                                                                                                                                                aTTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                <  2:3203601/61‑1 (MQ=255)
                                                                                                                                                aTTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacca                                                                            <  1:305747/81‑1 (MQ=255)
                                                                                                                                                aTTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAattccgcttagcagccaaggctgataagcaagatcgggtccagcaaaccggtcttgaaggtaagaaggt      >  1:1157067‑M1/1‑82 (MQ=255)
                                                                                                                                                    cGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGcc  >  1:2030255/1‑151 (MQ=21)
                                                                                                                                                    |                                                                                                                                                      
GATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAGCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCTACATTGCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCC  >  CP009974/3843979‑3844277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: