Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 542,104 C→G intergenic (‑365/+164) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974542,1040CG86.3% 78.7 / 14.9 51intergenic (‑365/+164)argD/RPPX_02300acetylornithine aminotransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/7);  new base G (6/38);  total (6/45)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

AGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATTTGCG  >  CP009974/541956‑542221
                                                                                                                                                    |                                                                                                                     
ggggaACAGGATGTACGTCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGa                                                                                                                     <  1:3220001/150‑1 (MQ=17)
 ggggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGat                                                                                                                    <  1:4729072/151‑1 (MQ=18)
 ggggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGat                                                                                                                    <  1:4729145/151‑1 (MQ=18)
  gggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGata                                                                                                                   >  2:2266883/1‑151 (MQ=18)
  gggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGata                                                                                                                   <  1:1983385/151‑1 (MQ=18)
    gACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATc                                                                                                                 <  1:1487643/151‑1 (MQ=18)
    gACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATc                                                                                                                 <  1:1487792/151‑1 (MQ=18)
    gACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATc                                                                                                                 <  2:5536/151‑1 (MQ=18)
       aGGATGTACGGCGATGTTGATGTGTTGGCGGGTAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAg                                                                                                              <  2:4424162/151‑1 (MQ=17)
        tcttGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGCGCGCCGCCCGCGCGGCGCTTCGCGGGTAAGCCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                                             <  1:1845468/148‑1 (MQ=17)
        ggATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                                             <  1:2266883/151‑1 (MQ=17)
        ggATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                                             >  1:2994349/1‑151 (MQ=17)
         gctGTACGGTGATGTTGATGTGTGCGCGCGGAGACCGCGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCGTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggt                                                                                                            <  2:4875417/149‑2 (MQ=14)
         gATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggt                                                                                                            <  1:3356929/151‑2 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGGCGGGATATCGAggtgt                                                                                                          >  2:1263122/1‑148 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                                          <  2:1955803/151‑4 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCCCCTGCGCCCGGCGCGCGCGACCGCCTCGTCTGTTCGACGGGAGATCGAggaga                                                                                                          >  2:1845468/1‑148 (MQ=17)
            gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                                         <  2:3779377/151‑5 (MQ=17)
            gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                                         <  1:4728859/151‑5 (MQ=17)
            gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                                         <  1:1423709/151‑5 (MQ=17)
              aCGGGGATGTTGATGTGTTGGCGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTgcg                                                                                                       <  1:4474010/151‑1 (MQ=14)
              aCGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtgcg                                                                                                       <  2:4356629/151‑7 (MQ=17)
                    aTGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAgg                                                                                                 <  1:3232601/151‑1 (MQ=14)
                     ggTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGc                                                                                                <  1:4568596/150‑1 (MQ=14)
                      gTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCg                                                                                               <  1:1218108/151‑1 (MQ=14)
                       ttGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGt                                                                                              <  1:4544614/151‑1 (MQ=14)
                        tGATGTGTTGGCGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                                             <  2:131273/151‑1 (MQ=14)
                        tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                                             <  2:3693187/151‑1 (MQ=14)
                        tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                                             <  1:1669614/151‑1 (MQ=14)
                        tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                                             <  2:1230604/151‑1 (MQ=14)
                         gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                                            <  1:1263122/151‑1 (MQ=14)
                         gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                                            <  1:1816659/151‑1 (MQ=14)
                         gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                                            <  1:3659702/151‑1 (MQ=14)
                          aTGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcg                                                                                           <  2:3608867/151‑1 (MQ=14)
                           tgtgTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgc                                                                                          <  2:2994349/151‑1 (MQ=14)
                             tgtTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgcgc                                                                                        <  2:1928986/151‑1 (MQ=11)
                                   ccgggAGATCGGCCGCCGCCCGCGTGGCGCTTCGCGGGTAGACCCTCTCCTACGCTTGTTTCGGGCCAATCCCTCCTGCGCCAGGCGCGCGCGACCGCCATGTATGTTCGACGGGAGATCGAGGTGTGCGGCAAGGCGTTCGCGCGCAAAt                                                                                  <  2:4015087/149‑1 (MQ=11)
                                   cGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAAt                                                                                  <  2:2667824/151‑1 (MQ=11)
                                    ggggAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATg                                                                                 <  1:2574871/151‑1 (MQ=11)
                                         gagcGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCcaca                                                                            <  1:1991167/148‑1 (MQ=0)
                                            cgggcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAGGc                                                                         <  2:1604987/148‑1 (MQ=0)
                                                       cgagcggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGCACCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGccc                                                              <  1:4205026/148‑1 (MQ=1)
                                                                ctgcgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGCACCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGCCCGaaacaaac                                                     <  1:3185244/148‑1 (MQ=1)
                                                                                gcgccTGCGTTTGTTTCGGGCCAGTCCCTCCTGCACCGGTCGCGCGCGCCCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTAc                                     <  2:1922187/148‑1 (MQ=1)
                                                                                       cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa                              <  1:4127392/148‑1 (MQ=1)
                                                                                       cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa                              <  1:4127480/148‑1 (MQ=1)
                                                                                       cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa                              <  2:3981971/148‑1 (MQ=1)
                                                                                        gtgtgtttCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTTTTTGTACGACGCAATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAAGCATAATTGGCCTGAAACAAGCGTAGGAGCGGGTTTACCCGCGAAg                             <  1:1796067/148‑1 (MQ=1)
                                                                                             ttgcGGGCCAGGCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTGGTACGACGCGATATCGAGACAGGCACCAAGGCGTTCGCGCGCGAATCCCACAGGCATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccg                        <  1:4614198/148‑1 (MQ=1)
                                                                                                                   cccggCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATTTgcg  >  1:4968101/4‑151 (MQ=1)
                                                                                                                   cccggCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCCCGATTTgcg  >  2:14472/4‑151 (MQ=1)
                                                                                                                                                    |                                                                                                                     
AGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATTTGCG  >  CP009974/541956‑542221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑