Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,651,471 A→G G393G (GGT→GGC RPPX_20730 ← 5,10‑methylene tetrahydromethanopterin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,651,4710AG95.8% 779.0 / 12.7 238G393G (GGT→GGCRPPX_207305,10‑methylene tetrahydromethanopterin reductase
Reads supporting (aligned to +/- strand):  ref base A (5/5);  new base G (125/103);  total (130/108)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GCAGCTGGCCGCCGCGGCCGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAACCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTCGGCGGCGGTGA  >  CP009974/4651323‑4651619
                                                                                                                                                    |                                                                                                                                                    
       gccgccGCGGCCGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATg                                                                                                                                             >  1:4015738/1‑151 (MQ=255)
             gcgGCCGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTa                                                                                                                                       <  2:4605574/151‑1 (MQ=255)
             gcgGCCGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTa                                                                                                                                       <  1:3085393/151‑1 (MQ=255)
               ggCCGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAgg                                                                                                                                     <  2:370518/151‑1 (MQ=255)
                 ccGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGt                                                                                                                                   >  1:5149532/1‑151 (MQ=255)
                  cGAACAGCTTGTGGGGCAGGGGGCCCTCGGTGGAGCGTAGCGGATAGCGGCGGCGCCTTTGCAGCTCGGGCACCAGCAGGTCGAATACAGTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTc                                                                                                                                  <  2:4064893/151‑1 (MQ=255)
                  cGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTc                                                                                                                                  >  2:438242/1‑151 (MQ=255)
                    aaCAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTcca                                                                                                                                <  1:4491767/151‑1 (MQ=255)
                    aaCAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTcca                                                                                                                                >  2:4667784/1‑151 (MQ=255)
                    aaCAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGAAGAAGCCGTCGATGCCGGTAGGGTcca                                                                                                                                >  2:1262303/1‑151 (MQ=255)
                              ggCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTc                                                                                                                      <  2:3807436/151‑1 (MQ=255)
                                cgcAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCa                                                                                                                    >  1:872550/1‑151 (MQ=255)
                                cgcAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCa                                                                                                                    <  2:1447574/151‑1 (MQ=255)
                                 ggaGGGGGCCGTCGGTGTGGCGCCGCGGGTAGCGGGCGCGGCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTGCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAg                                                                                                                   <  2:3998483/149‑1 (MQ=255)
                                  cAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAACCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGc                                                                                                                  <  1:2617241/151‑1 (MQ=255)
                                   aGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTCCATCGGCCAGGTCGACCGGTAGCACGGCGTGGAGCAGGATGGTGCCGTCGATGCCGGTCGGCTCCTGCCAGGATACCAGCt                                                                                                                 >  2:1089288/1‑151 (MQ=255)
                                   aGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCt                                                                                                                 >  2:286965/1‑151 (MQ=255)
                                   aGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCt                                                                                                                 >  1:3747992/1‑151 (MQ=255)
                                     ggggCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCACGTCGTCCGGTTGCACGGCGTAGGTCAGGGTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTgg                                                                                                               >  2:3315929/1‑151 (MQ=255)
                                        gCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCg                                                                                                            >  2:2400049/1‑151 (MQ=255)
                                          ccTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGc                                                                                                          <  1:4325818/151‑1 (MQ=255)
                                          ccTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGc                                                                                                          >  1:4254857/1‑151 (MQ=255)
                                           cTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGcc                                                                                                         <  2:4410272/151‑1 (MQ=255)
                                             cGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCAc                                                                                                       >  2:3011729/1‑151 (MQ=255)
                                                 gtAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCg                                                                                                   >  2:1286204/1‑151 (MQ=255)
                                                 gtAGCTCAGCGGGGAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCg                                                                                                   <  1:3408382/151‑1 (MQ=255)
                                                    gCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTg                                                                                                <  1:1665365/151‑1 (MQ=255)
                                                         gCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAgc                                                                                           <  2:593394/151‑1 (MQ=255)
                                                            ggTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGcc                                                                                        <  1:1147557/151‑1 (MQ=255)
                                                             gTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCg                                                                                       >  1:3870285/1‑151 (MQ=255)
                                                               aGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGAt                                                                                     <  2:2474024/151‑1 (MQ=255)
                                                                  ggCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAg                                                                                  >  1:3513416/1‑151 (MQ=255)
                                                                      gcgcCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACg                                                                              >  1:805492/1‑151 (MQ=255)
                                                                           gTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGcc                                                                         <  2:1393594/151‑1 (MQ=255)
                                                                            ttGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGCTCAGCACGGGGCcg                                                                        >  2:4518544/1‑151 (MQ=255)
                                                                             tGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCcgc                                                                       <  1:4621845/151‑1 (MQ=255)
                                                                              gCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCcgcg                                                                      >  2:3353740/1‑151 (MQ=255)
                                                                               cAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGAGCAGCACGGGGCCGCGa                                                                     >  1:135318/1‑151 (MQ=255)
                                                                                aGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCcgcgcc                                                                    >  2:3752744/1‑149 (MQ=255)
                                                                                aGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGAc                                                                    <  1:1933744/151‑1 (MQ=255)
                                                                                aGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGCTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGCCCGCGAc                                                                    >  2:4161630/1‑151 (MQ=255)
                                                                                  cTCGCGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGCCCg                                                                  >  2:4317843/1‑151 (MQ=255)
                                                                                     gggCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGccc                                                               >  2:3920396/1‑151 (MQ=255)
                                                                                     gggCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGccc                                                               >  2:4682207/1‑151 (MQ=255)
                                                                                     gggCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAACCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGccc                                                               >  2:352957/1‑151 (MQ=255)
                                                                                       gCACCAGCAGGTCGACTGCATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAg                                                             <  2:2553938/151‑1 (MQ=255)
                                                                                       gCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAg                                                             <  2:1951517/151‑1 (MQ=255)
                                                                                        cACCAGCAGGTCGGCTACATGGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAgg                                                            <  2:4233542/151‑1 (MQ=255)
                                                                                        cACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAgg                                                            >  2:2146089/1‑151 (MQ=255)
                                                                                        cACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAgg                                                            >  1:2280312/1‑151 (MQ=255)
                                                                                        cACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAgg                                                            >  1:3267667/1‑151 (MQ=255)
                                                                                            agcagGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCaa                                                        <  1:2438659/151‑1 (MQ=255)
                                                                                            agcagGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCaa                                                        >  1:2721333/1‑151 (MQ=255)
                                                                                             gcagGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAAc                                                       >  2:2124157/1‑151 (MQ=255)
                                                                                              cagGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACg                                                      <  1:610288/151‑1 (MQ=255)
                                                                                              cagGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCCACCGCCCAGGCCAACg                                                      >  2:183215/1‑151 (MQ=255)
                                                                                               agGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGa                                                     >  1:2086404/1‑151 (MQ=255)
                                                                                                 gTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAAc                                                   >  1:3949872/1‑151 (MQ=255)
                                                                                                 gTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAAc                                                   <  1:2366854/151‑1 (MQ=255)
                                                                                                  tCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACt                                                  <  2:577994/151‑1 (MQ=255)
                                                                                                  tCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACt                                                  <  1:1369708/151‑1 (MQ=255)
                                                                                                   cGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTc                                                 <  1:1714547/151‑1 (MQ=255)
                                                                                                       tACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTcggcg                                             >  1:4820996/1‑151 (MQ=255)
                                                                                                        aCATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTcggcgg                                            >  1:2695655/1‑151 (MQ=255)
                                                                                                          aTTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCAACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCt                                          <  1:406704/151‑1 (MQ=255)
                                                                                                           ttGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCtt                                         <  2:4897908/151‑1 (MQ=255)
                                                                                                            tGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTc                                        >  2:3187469/1‑151 (MQ=255)
                                                                                                              gCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTcgc                                      >  2:1880569/1‑151 (MQ=255)
                                                                                                                cAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGt                                    <  1:2398841/151‑1 (MQ=255)
                                                                                                                 aGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTa                                   >  1:4230151/1‑151 (MQ=255)
                                                                                                                   gtcgtcCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACg                                 >  1:3443965/1‑151 (MQ=255)
                                                                                                                      gtcCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTc                              >  1:1836184/1‑151 (MQ=255)
                                                                                                                       tcCGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGCCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTcc                             >  2:5061987/1‑151 (MQ=255)
                                                                                                                        ccGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCa                            >  1:120758/1‑151 (MQ=255)
                                                                                                                        ccGGTTGCACGGCGTAGGTCAGGTTGAAACCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCa                            <  1:3331229/151‑1 (MQ=255)
                                                                                                                         cGGTTGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAg                           <  1:4930694/151‑1 (MQ=255)
                                                                                                                           gTTGCACGGCGTGGGTCAGGTTGGAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAgcg                         <  2:2755408/151‑1 (MQ=255)
                                                                                                                            ttGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAgcgg                        >  1:3411402/1‑151 (MQ=255)
                                                                                                                             tGCACGGCGTAGGTCAGGTTGAGGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAgcggc                       <  1:1628709/151‑1 (MQ=255)
                                                                                                                             tGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGCCCGCCCAGGCCAACGAACTCGGCGGCGTCGCGTACGGTCCAgcggc                       >  2:4211598/1‑151 (MQ=255)
                                                                                                                             tGCACGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAgcggc                       >  2:1221004/1‑151 (MQ=255)
                                                                                                                              gcgcggcggAGGTGGGGTGGAAGCCGTCGGTGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAgcggcg                      <  2:2374645/148‑1 (MQ=255)
                                                                                                                               cACGGCGGAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGGCCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTCCGGTCCAGCGGCGa                     >  1:1039636/1‑151 (MQ=255)
                                                                                                                                aCGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAg                    <  1:5011261/151‑1 (MQ=255)
                                                                                                                                 cGGCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGTCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAgg                   >  2:798532/1‑150 (MQ=255)
                                                                                                                                   gCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTgg                 <  2:866659/151‑1 (MQ=255)
                                                                                                                                   gCGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTgg                 <  1:1518458/151‑1 (MQ=255)
                                                                                                                                    cGTAGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTggg                <  1:516188/151‑1 (MQ=255)
                                                                                                                                       aGGTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTc             <  1:3741917/151‑1 (MQ=255)
                                                                                                                                        ggTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcg            >  1:3793001/1‑151 (MQ=255)
                                                                                                                                         gTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcgg           <  1:5116738/151‑1 (MQ=255)
                                                                                                                                         gTCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcgg           <  1:5231521/151‑1 (MQ=255)
                                                                                                                                          tCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggc          >  1:2052344/1‑151 (MQ=255)
                                                                                                                                          tCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggc          <  1:286965/151‑1 (MQ=255)
                                                                                                                                          tCAGGTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggc          <  1:286320/151‑1 (MQ=255)
                                                                                                                                             ggTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggcggc       <  1:3233669/151‑1 (MQ=255)
                                                                                                                                             ggTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGAGACCGCCCAGGCCAGCGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggcggc       >  2:5013823/1‑151 (MQ=255)
                                                                                                                                              gTTGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggcggcg      >  1:4127095/1‑151 (MQ=255)
                                                                                                                                               ttGAAGCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTcggcggcgg     >  2:4757256/1‑151 (MQ=255)
                                                                                                                                                  aaaCCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTCGGCGGCGGTGa  >  2:1590005/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGCTGGCCGCCGCGGCCGAACAGCTTGTGGCGCAGGGGGCCCTCGGTGTAGCTCAGCGGGTAGCGGCCGCGCCGTTGCAGCTCGGGCACCAGCAGGTCGACTACATTGGCCAGGTCGTCCGGTTGCACGGCGTAGGTCAGGTTGAAACCGTCGATGCCGGTAGGGTCCAGCCAGGTTTCCAGCTGGTCGGCCACCTCGCTGGCAGCGCCGATCAGCACGGGGCCGCGACCGCCCAGGCCAACGAACTCGGCGGCTTCGCGTACGGTCCAGCGGCGAGTGGGGTCGGCGGCGGTGA  >  CP009974/4651323‑4651619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: