| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,524,728 | 0 | T | G | 48.0% | 8.9 / 11.2 | 25 | P68P (CCA→CCC) | CJ019_01439 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/6); new base G (0/12); total (7/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGG > ACB122/1524594‑1524863 | cAGGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTg < 8:201296/136‑1 (MQ=255) acaTGGCAGGGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCGGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCgg < 2:221913/136‑1 (MQ=255) ctgggCGGTGCGCGCGGGCAGCCGTTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCACCTGGCGCGGGGCCTGGTTCAGCAGCTGGCGCAGCGGGCGAACCGGGCGCAGCTGGGCGTATGGTGGCTGgggggg < 4:239810/133‑1 (MQ=255) caTGGCTGTGCGGGCGGGCAGCCGGTTGCAACTGGCTTTCCCGCAGGCGCGGCATGTGCGCCCGGCGCTCGGCCTGGTTCCGCAGCAGGCGCAGCGGGCGCACGTGGCGCAGCAGGGCGGAAGGGggcggggcgg < 5:49784/135‑1 (MQ=255) aTGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCggg > 6:220689/1‑135 (MQ=255) gCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGAAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGGGGCTGGGCGGGGGCGGGttt < 3:84633/136‑1 (MQ=255) cgcgcgGGCAGCCGGTTGCAACTGGCTTGCCAGCTGGCGCTGAAAGTGCTCCCGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCCGGGCGTAAGGTGGCTGGGCGGGGGAGGGttt < 5:211980/133‑1 (MQ=255) tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGttgg > 6:185240/1‑133 (MQ=255) tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg > 2:186035/1‑135 (MQ=255) gCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCa > 2:19029/1‑136 (MQ=255) ccGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATgg > 5:199159/1‑135 (MQ=255) tggaaaCGGGATTGCCAGATGGCGCTTAACGGGCGCCCGGCGCTCGGCGTGGTACCGCATCAGGCGCAGGGGGCGAAACGGGCGCAGAAGGGCGTAAGGGGGCTGGGCGTGGGAGGGTTTGTAGGCCATGGGGGTa < 8:219208/132‑1 (MQ=255) ggatttCCAGCAGGCGTGGCTGTTGAGCCAGGCGGTGGGCCGTGTACCCAAGAAGGCGCAGCGGTCCCACCTGGCGCAGCAGGGCGTAAGGTGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa < 4:12229/132‑1 (MQ=255) ccgccggCGCTGAACGTGCGCCCGGGGCGCGGCGTGGTCCTGCCGCCGGCGCCGCGGGCCCCCCGGGCGCCGCGGGGCGTATGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt < 7:152656/131‑1 (MQ=255) cagccgGCGCGGCAAGGGCTCCCGGCGCGCGGCCGGGTTCAGCAGCAGGCGCCGGGGGCGCACCTGGGGCACCCGGGCGGAAGGGGGCGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt < 2:105773/136‑1 (MQ=255) gccggCGCTGCATGTGCTCCAGGCGCGCGGCCGGGTTCAGCAGCCGGCGCAGCGGGCGCACGGGGCGCAGCCGGGCGTATGGGGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg < 1:95445/133‑1 (MQ=255) gccggCGCTGAATGTGCTCCCGGGGCGGGGCGTGTTGCCGCTGCCGGCGCAGGGGGCGCACCTGGCGCAGCAGGGCGGAAGGGGGCGGGGCGGGGTCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg < 8:225458/133‑1 (MQ=255) cagGCGCTGCATGTGCACCCGGCGCTCGGCGTGGTACAGCAGCAGCCGCTGGGGGCGCAGCTGGCGCAGCAGGCGGTAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg < 6:52278/135‑1 (MQ=255) cAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTcc > 5:232313/1‑135 (MQ=255) cggCGCTCGGCCGGGCTCCCCCGCCGGCGCCGCGGGCGCACCGGGCGCAGCCGGGCGGAAGGGGGCGGGGCGGGGGTGGGTTGGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt < 3:342/135‑1 (MQ=255) ccTGGTGCCGCCGCGGGGGCAGGGGGCGCACGTGGCGCAGCTGGGGGGTGGGGGGCTGGGGGGGGGTGGGTGTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGcc < 4:172714/136‑1 (MQ=255) agcagGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTcgc > 3:172714/1‑136 (MQ=255) gCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAccc < 7:128855/136‑1 (MQ=255) gcACCTGGCGCAGCAGGGCGGAAGGGGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTcc < 6:59640/135‑1 (MQ=255) cagctgGGGGGATGGGGGGGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTa < 3:91365/136‑1 (MQ=38) tGGCGGGGCGGGGGAGGGTTTGGAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTACCATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAgg < 1:93179/136‑1 (MQ=255) | CAGGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGG > ACB122/1524594‑1524863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |