Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,086,5790AC52.9% 2.7 / 11.9 17intergenic (‑307/+30)aruC/cdhR_7Succinylornithine transaminase/acetylornithine aminotransferase/HTH‑type transcriptional regulator CdhR
Reads supporting (aligned to +/- strand):  ref base A (5/3);  new base C (9/0);  total (14/3)
Fisher's exact test for biased strand distribution p-value = 8.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCACCAAAGA  >  ACB122/5086452‑5086697
                                                                                                                               |                                                                                                                      
gggggCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCGGGCTTTGGGGCCCCACCCCAACCCCCAGGCCTac                                                                                                                >  7:210562/1‑136 (MQ=255)
gggggCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCGCGATTTGCGCCCCCACCCCAACCCCAAGGCCTac                                                                                                                >  7:161959/1‑136 (MQ=255)
  gggCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCCAGGCATacac                                                                                                              >  1:54714/1‑136 (MQ=255)
  gggCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGAGCCGCCTGGGCGGCGCTCGTTTTGCGCCCCAACACCAACCCCAACGCAAaccc                                                                                                              >  1:90168/1‑134 (MQ=255)
       gggTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGCGCGCCGCGGGGGGGGCGCGGGTTTTGCGGCCCCACCCCACCCCCCAGGCCTACACCCCgc                                                                                                         >  1:220923/1‑135 (MQ=255)
       gggTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGGGGGCCGCGCGCGATTTGCGCCCCCACCCCCACCCCCACGCCTACCCCCCgc                                                                                                         >  3:199543/1‑135 (MQ=255)
                              aTTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGACCGCCGCGTGGGCGGCGCGCGATTTTCGCCCCCACACCAACCCCAATGCCTAGCACCCGCACCCCCCCGAAAACACAGcccct                                                                                  >  1:8526/1‑134 (MQ=255)
                                   gAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGCGCGCCGCGTGGGCGGCGCTCGCTTTGCGCCCCACCCCCCACCCCCAGGCATACACCCCGCACCCCCCCGCAATCTGAGcccggttga                                                                              >  5:65369/1‑129 (MQ=255)
                                          cAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGGTTTTCGCCCCCACCCCAACCCCCAGGCATGCACCCCCCCCCCCCCCGCAATCTCAGCCCGTTTCAGGCGgcgc                                                                      >  7:181804/1‑136 (MQ=255)
                                          cAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCCAGGCCTACACCCCCCACCCCCCCGCAATCTCACCCCGTTTAAGCCGgcg                                                                       >  6:190317/1‑135 (MQ=255)
                                          cAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCCCGTGGGCGGCGGTCGCTTTTCGCCCCACCCCCCCCCCCCAGGCCTTCCCCCCCCCCCCCCCCGCAATCTCACCCCGTTTGCGCCCgcgc                                                                      >  7:18347/1‑136 (MQ=255)
                                                      cGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCGCGATTTGCGCCCCACCCCCCACCCCCACGCATACCCCCCGCCCCCCCCCGCAATCGCAGCCCGTTTAAGCCGGCGCCGGGCTCAAtt                                                           >  7:196076/1‑135 (MQ=255)
                                                        aTGTTGAGGTGTTGCGGGGGAGCTCGCGCGCCGGGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCaa                                                        <  8:189667/136‑1 (MQ=255)
                                                           ttgtggtgtGGACGGGGCGATCGCGCGCCGCGGGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACg                                                     <  2:54714/131‑1 (MQ=255)
                                                                                                             cgcCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCACCAAAg   <  8:207741/136‑1 (MQ=255)
                                                                                                               ccccAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCACCAAAGa  >  3:61293/1‑135 (MQ=255)
                                                                                                               ccccAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCACCAAAGa  >  3:102536/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                      
GGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCACCAAAGA  >  ACB122/5086452‑5086697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: