Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 2,856,886 T→G S297A (TCG→GCG)  hdfR_4 → HTH‑type transcriptional regulator HdfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,856,8860TG100.0% 11.3 / NA 11S297A (TCG→GCG) hdfR_4HTH‑type transcriptional regulator HdfR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (1/10);  total (1/10)

ACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGG  >  ACB122/2856758‑2857012
                                                                                                                                |                                                                                                                              
aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGCGGCGCGCCGGc                                                                                                                         >  8:1377/1‑136 (MQ=255)
                                                  gggggcCACCGGCGGTTGCACACTCCCCGCCCGCGCACGGGGGTGCCCCAGGCGCGGTGGGTGTGGCCGCGGGCGGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt                                                                       <  7:1377/135‑1 (MQ=255)
                                                   gggggCCCCGGCTATTCCACCCAATCCAGACGCGCACGGGCGAGCCCCGGGCGCGGTGTGGGTTGCGGGGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt                                                                       <  6:212959/135‑1 (MQ=255)
                                                            cGCTGTCCCACCCTCACCTCCCGCTCCCGGGCGGGCACCCGGCGGGGGGGGGGTGGCCGGGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCggtggt                                                             <  1:26796/136‑1 (MQ=255)
                                                                              tgccGCTCCGGGGCGGGCCCCTGGCGGGGTGGGTGTGGCGGGGGGCGGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTgg                                            <  6:10435/132‑1 (MQ=255)
                                                                                    tCACGGGGGTGCACCAGGGGCGGGGGGTGTTGCGGCGGGCGGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                     <  2:144208/136‑1 (MQ=255)
                                                                                    gcaggggcgggcCCCTGGGGCGCGGGGTGGGGCGGGGGGCCGGGGCGCGGGCCGGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                     <  3:87519/132‑1 (MQ=255)
                                                                                     cccgggCGTGCCCCCGGCGCGGGGGGGGTGGCCGCGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                     <  5:114665/132‑1 (MQ=255)
                                                                                      acgggCGCGCACCAGGCGGGCTGGGTGTTGCCGGGGGCGGGCGCGCCGGCCCCTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                     <  3:89587/132‑1 (MQ=255)
                                                                                        gggcgggcACCAGGCGCGGGGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGt                                 <  3:78052/136‑1 (MQ=255)
                                                                                                                       ggggCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTggcgg  <  3:61311/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                              
ACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGG  >  ACB122/2856758‑2857012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: