Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,287,386 G→A 6.1% G323G (GGC→GGT mmsA ← Methylmalonate‑semialdehyde dehydrogenase[acylating]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,287,3860GA 6.1% 218.6 / 3.7 68G323G (GGC→GGTmmsAMethylmalonate‑semialdehyde dehydrogenase[acylating]
Reads supporting (aligned to +/- strand):  ref base G (40/23);  new base A (2/2);  total (43/25)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

ACCTTGACGTCACGGCCGTCGAGCTCGAGCTTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCCCACCAGC  >  ACB122/5287261‑5287518
                                                                                                                             |                                                                                                                                    
aCCTTGACGTCACGGCCGTCGAGCTCGAGCTTGGCGCCTTCCGTGCTACCGCTTTCGATCAGGCCCCGCACACGCTCCTTGGCACGCTTGGAACCCACCGGGCCCACGTCGGTGCCCGGCTCCCCGCCCGCATTg                                                                                                                             >  3:78993/1‑135 (MQ=255)
aCCTTGACGTCACGGCCGTCGAGCTCGAGCTTGGCGCCGTCCTTGAGACCGCGTTCGATCAGGCCCAGCACCCGCTCCTTGGCACGCTTGGAAACCACCGGGCCCACGTCGGTGCCCGGCGCCCACCCAGCATTg                                                                                                                             >  5:223814/1‑135 (MQ=255)
      aCGTCACGGCCGTCGAGCTCGAGCTTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAATCCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCtt                                                                                                                        <  6:2849/134‑1 (MQ=255)
       cGTCACGGCCGTCGAGCTCGAGCTTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCACCGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTgc                                                                                                                      >  7:310435/1‑134 (MQ=255)
                 gTCGAGCTCGAGCTTGGCGCCTTCCTTGATAGCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTggc                                                                                                            <  3:53445/135‑1 (MQ=255)
                 gTCGAGCTCGAGCTTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTggc                                                                                                            <  3:35177/135‑1 (MQ=255)
                 gTCGAGCTCGAGCTTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTggc                                                                                                            <  3:364278/135‑1 (MQ=255)
                     agctcgagctTGGCGCCTTCCTTGATACCGCTTTAGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACCGCCCGCATTGACCTTGAGCTTGCTGGCGGcc                                                                                                        >  2:99036/1‑135 (MQ=255)
                            gctTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGa                                                                                                 >  2:78424/1‑135 (MQ=255)
                             ctTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGat                                                                                                <  8:248043/135‑1 (MQ=255)
                                 gcgcCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATc                                                                                             <  2:63953/134‑1 (MQ=255)
                                          ttGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGACTCCGTGATATCCGGCAACCAt                                                                                   >  4:92056/1‑135 (MQ=255)
                                               aCCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCGCCTTGATATCCGGCAACCATTcgcg                                                                              >  8:84709/1‑135 (MQ=255)
                                                    tttCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCt                                                                         >  8:307816/1‑135 (MQ=255)
                                                      tCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTg                                                                       >  7:86917/1‑135 (MQ=255)
                                                      tCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTg                                                                       >  1:287285/1‑135 (MQ=255)
                                                      tCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTg                                                                       >  4:185897/1‑135 (MQ=255)
                                                      tCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTg                                                                       >  3:105162/1‑135 (MQ=255)
                                                      tCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTg                                                                       >  5:112641/1‑135 (MQ=255)
                                                      tCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGCCCAACTTCGCTGCCCGCCTCACAGCCAGCATTGACCATGAGCTTGCTCGCGGCCTCCTTGATATCCCGCAACCAAACGCGGGCCTTg                                                                       >  2:244611/1‑135 (MQ=255)
                                                         aTCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGcc                                                                     <  6:180730/134‑1 (MQ=255)
                                                             ggCCCCGCACACGCTCCTTGGCACGGATGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCccacca                                                                <  8:104085/135‑1 (MQ=255)
                                                              gCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCaa                                                               <  2:224323/135‑1 (MQ=255)
                                                                 cAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAacac                                                            >  6:127955/1‑135 (MQ=255)
                                                                  aGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACg                                                           <  2:31869/135‑1 (MQ=255)
                                                                       acGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCAc                                                      >  3:208949/1‑135 (MQ=255)
                                                                         gcgccTTGCCACGCTAGCAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGACAGCATTGCCCTGGCGCTTCCTGGCGGCCTCCTTGATACCCGGAAACCATTCGCGGGCCTTGCCCACCAACACGGCCACgg                                                    <  1:334699/132‑1 (MQ=255)
                                                                         gCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACgg                                                    <  6:223814/135‑1 (MQ=255)
                                                                           tCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCACCCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAg                                                  >  3:240906/1‑135 (MQ=255)
                                                                           tCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAac                                                              <  1:248077/123‑1 (MQ=255)
                                                                           tCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAac                                                              >  2:248077/1‑123 (MQ=255)
                                                                           tCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAg                                                  >  7:318938/1‑135 (MQ=255)
                                                                           tCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAg                                                  >  8:39325/1‑135 (MQ=255)
                                                                            ccTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCCCCAACCCGGCTACGGAgg                                                 >  1:129095/1‑135 (MQ=255)
                                                                            ccTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCACCACGGCCACGGAgg                                                 >  1:292125/1‑135 (MQ=255)
                                                                            ccTTGGCACGCTTGGAAACAACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCCCCAACACGGCCACGGAgg                                                 >  1:11287/1‑135 (MQ=255)
                                                                              ttGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAgg                                                 <  3:305914/133‑1 (MQ=255)
                                                                                  cACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGcc                                            <  6:67161/134‑1 (MQ=255)
                                                                                   aCGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCAt                                          >  6:288868/1‑135 (MQ=255)
                                                                                      cTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCa                                       >  6:369442/1‑135 (MQ=255)
                                                                                        tGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCACCCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAgc                                     >  2:264267/1‑135 (MQ=255)
                                                                                          gAAACCACCGTGCCAACTTAGTTGCACGTCTAACAGCCAGCATTTACCTTGATCTTGCTGGCGGCCTCCTTGATATCAGGCAACCATTCGCGTGCCTTGCCCTACTACACGGCAACGGAGGTCGCCATTCAgcgc                                   >  7:244004/1‑135 (MQ=255)
                                                                                          gAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCACCACGGCCACGGAGGTCGCCAGGCAgcgc                                   >  1:98402/1‑135 (MQ=255)
                                                                                          gAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACCCGGCCACGGAGGTCGCCATGCAgcgc                                   >  6:61858/1‑135 (MQ=255)
                                                                                          gAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAgcgc                                   >  2:201096/1‑135 (MQ=255)
                                                                                                  cGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCgg                                                                                          <  4:168550/72‑1 (MQ=255)
                                                                                                  cGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCgg                                                                                          >  3:168550/1‑72 (MQ=255)
                                                                                                  cGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCccgc                           >  8:199499/1‑135 (MQ=255)
                                                                                                  cGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCccgc                           >  4:182657/1‑135 (MQ=255)
                                                                                                    ggCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCCACACGGCCCCGGAGGTCGCCATGCAGCGCTGGCccgccg                         >  5:309021/1‑135 (MQ=255)
                                                                                                    ggCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCccgccg                         >  2:200955/1‑135 (MQ=255)
                                                                                                    ggCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCccgccg                         >  4:32020/1‑135 (MQ=255)
                                                                                                    ggCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCccgccg                         >  6:297759/1‑135 (MQ=255)
                                                                                                       cAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCcgcg                       >  8:86945/1‑134 (MQ=255)
                                                                                                               gTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCa              <  4:76462/135‑1 (MQ=255)
                                                                                                               gTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCa              <  2:287285/135‑1 (MQ=255)
                                                                                                                tGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAg             <  3:182657/135‑1 (MQ=255)
                                                                                                                 gCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGc            >  3:257952/1‑135 (MQ=255)
                                                                                                                  cccGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGcc           >  4:15033/1‑135 (MQ=255)
                                                                                                                  cccGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGcc           >  3:30417/1‑135 (MQ=255)
                                                                                                                  cccGGCTCACAACCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGcc           >  2:175012/1‑135 (MQ=255)
                                                                                                                  cccGGCTCACAACCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCGTGATATCCGGCAACCATTCGCGGGCCGTGCCCCCCAACCCCGCCACGGAGGTCGCCAAGCAGCGCTGGCCCGCCGCGCCCAACGCAccc           >  2:180038/1‑135 (MQ=255)
                                                                                                                    cGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCGTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGcccc         <  7:39325/135‑1 (MQ=255)
                                                                                                                       cTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCccac      <  5:39556/135‑1 (MQ=255)
                                                                                                                       cTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCccac      <  4:105162/135‑1 (MQ=255)
                                                                                                                       cTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCccac      <  8:310435/135‑1 (MQ=255)
                                                                                                                       cTCACAACCCGCATTGACCTTGAGGTTGCTGGGGGCCTCCTTGATATCCGGCAACAATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCccac      <  1:180038/135‑1 (MQ=255)
                                                                                                                       cTCACAACCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCccac      <  1:175012/135‑1 (MQ=255)
                                                                                                                           cagccagcATTGACCTTGAGCTTGCTGGCGGCGTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCCCACCAgc  <  6:170470/135‑1 (MQ=255)
                                                                                                                             |                                                                                                                                    
ACCTTGACGTCACGGCCGTCGAGCTCGAGCTTGGCGCCTTCCTTGATACCGCTTTCGATCAGGCCCAGCACACGCTCCTTGGCACGCTTGGAAACCACCGGGCCAACGTCGGTGCCCGGCTCACAGCCAGCATTGACCTTGAGCTTGCTGGCGGCCTCCTTGATATCCGGCAACCATTCGCGGGCCTTGCCCACCAACACGGCCACGGAGGTCGCCATGCAGCGCTGGCCCGCCGCGCCAAAGGCAGCCCCCACCAGC  >  ACB122/5287261‑5287518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: