Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,101,657 C→T 5.7% G213R (GGA→AGA)  yeiR ← Zinc‑binding GTPase YeiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,101,6570CT 5.7% 223.6 / 2.5 70G213R (GGA→AGA) yeiRZinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand):  ref base C (39/27);  new base T (2/2);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAGCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATC  >  ACB122/6101529‑6101790
                                                                                                                                |                                                                                                                                     
tttCAGCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCTCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGAtt                                                                                                                                 <  4:255535/135‑1 (MQ=255)
     gCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCTGCAATTACTCCAACAACCCTCTCCCTGCTGCACATGGCAATTCGGTTTAGTCGGGTCAGCCGAGATTGATGCTGGAGCGTTTCTATGTTTGtgg                                                                                                                             >  6:266695/1‑132 (MQ=255)
     gCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCTAAGATTGTCg                                                                                                                             >  6:266700/1‑134 (MQ=255)
                    ccTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCa                                                                                                             <  2:371290/134‑1 (MQ=255)
                      tgttgtCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCaa                                                                                                            >  6:53368/1‑134 (MQ=255)
                       gttgtCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGa                                                                                                          >  4:297340/1‑135 (MQ=255)
                         tgaccAGGGTGCCACCGCCAGCCAATACTCCAACCACCATCTCCCTCCTGGACAAGGCAAATCGGTACACTCGGGTCACCCCACAATGCTGCTGGAGCGATTCCAACAATGACGACAATCCTGTTTATCAAGAAt                                                                                                        >  7:245407/4‑135 (MQ=255)
                           tCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  1:10803/134‑1 (MQ=255)
                           tCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  6:315844/134‑1 (MQ=255)
                           tCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTACCTGCTTGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  5:15500/134‑1 (MQ=255)
                           tCCAGGGTGCCACCGCCAGCCAATACTCCAACAACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATgg                                                                                                      >  2:158239/1‑135 (MQ=255)
                                ggTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTg                                                                                                  >  8:159661/1‑134 (MQ=255)
                                ggTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTg                                                                                                  >  7:235223/1‑134 (MQ=255)
                                 gggCCACCGCCATCCAATACTCAAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGGTGGCTCGGGTCAGCCCACAATGCTGCTTGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTc                                                                                                <  8:225372/133‑1 (MQ=255)
                                 gTGCCAACGCCAGCAAATACTCCAACAACCCGCTCCCTGCGGGACAAGGCAAATCGGTTGACTCGGGGCAGCCCACAATGCTGCTGTAGCGATTCCAAGATTGTGGAGAATCCTGTTTTTCAAGAATGGGCTGTc                                                                                                <  6:242308/135‑1 (MQ=255)
                                     cACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTcaaca                                                                                            >  3:245059/1‑135 (MQ=255)
                                        cGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATc                                                                                         >  6:325187/1‑135 (MQ=255)
                                        cGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATc                                                                                         >  6:299525/1‑135 (MQ=255)
                                             gccaATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCtgt                                                                                      >  3:11728/1‑133 (MQ=255)
                                              ccaATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGga                                                                                   <  5:129416/135‑1 (MQ=255)
                                               caATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGgag                                                                                  <  2:343600/135‑1 (MQ=255)
                                                     tCCAACCACCCTCTCCCTGCTCGACAAGGCAAAACGCTTGACTCGGGTCAGCCCACAATGCTCCTCGAGCGATTCCAAGATTGTCGACAATCATGTTTTTCAAGAAGATGATGTCAAAAATCTCTGGAGATAcct                                                                            >  2:280987/1‑134 (MQ=255)
                                                          ccaccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCg                                                                         >  7:317178/1‑133 (MQ=255)
                                                          ccaccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCg                                                                         >  6:372715/1‑133 (MQ=255)
                                                           caccCTCTCCCTGCTGGAGAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      <  5:5158/135‑1 (MQ=255)
                                                           caccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      >  2:254918/1‑135 (MQ=255)
                                                           caccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      >  7:321147/1‑135 (MQ=255)
                                                           caccCTCTCCCTGCTGGACAAGGCAAATCGGGTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      <  4:26587/135‑1 (MQ=255)
                                                            accCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAg                                                                     >  4:353342/1‑135 (MQ=255)
                                                            accCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAg                                                                     >  1:325267/1‑135 (MQ=255)
                                                                tctcCCTGCTTGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCTAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACTATCTGTGGAGATACCGTCGGGAGGCCg                                                                 <  5:266695/135‑1 (MQ=255)
                                                                tctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCTAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCg                                                                 <  5:266700/135‑1 (MQ=255)
                                                                tctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGcc                                                                  >  6:225477/1‑134 (MQ=255)
                                                                 ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATgg                                                                                                      >  1:10764/1‑97 (MQ=255)
                                                                 ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATgg                                                                                                      <  2:10764/97‑1 (MQ=255)
                                                                 ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCgg                                                                >  5:190978/1‑135 (MQ=255)
                                                                 ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATCCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTAGGGAGGCCgg                                                                >  3:228612/1‑135 (MQ=255)
                                                                           gACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGAc                                                              >  8:15128/1‑127 (MQ=255)
                                                                           gACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGAc                                                              <  7:15128/127‑1 (MQ=255)
                                                                           gACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAg                                                      >  6:262887/1‑135 (MQ=255)
                                                                            aCAAGGCAAATCGGGTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCa                                                       <  5:123880/133‑1 (MQ=255)
                                                                                   aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACTATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  5:86059/135‑1 (MQ=255)
                                                                                   aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGCCAAAGGCAGCTGCCCtt                                              >  2:282231/1‑135 (MQ=255)
                                                                                   aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  3:138276/135‑1 (MQ=255)
                                                                                   aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              >  6:53417/1‑135 (MQ=255)
                                                                                   aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  8:321147/135‑1 (MQ=255)
                                                                                   aaaTCGGATGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  6:34028/135‑1 (MQ=255)
                                                                                   aaaGCGGTTGACTCGGGGCAGCCCACAAGGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  5:220626/135‑1 (MQ=255)
                                                                                        ggTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACg                                         >  7:91727/1‑135 (MQ=255)
                                                                                        ggTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACg                                         >  8:54794/1‑135 (MQ=255)
                                                                                             aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       >  5:360144/1‑68 (MQ=255)
                                                                                             aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  6:360144/68‑1 (MQ=255)
                                                                                             aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTcc                                     <  6:142139/134‑1 (MQ=255)
                                                                                             aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGACGGACAAAGGCAGCTGCCCTTGCACGGTcc                                     >  7:373031/1‑134 (MQ=255)
                                                                                               tCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCcaca                                  >  4:344354/1‑135 (MQ=255)
                                                                                                  ggTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        <  8:81172/110‑1 (MQ=255)
                                                                                                  ggTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        >  7:81172/1‑110 (MQ=255)
                                                                                                      aGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGAtctt                           >  7:287445/1‑135 (MQ=255)
                                                                                                           acaATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCttctttt                      >  7:183897/1‑135 (MQ=255)
                                                                                                            caATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCttcttttc                     <  3:344354/135‑1 (MQ=255)
                                                                                                                gctgctGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCCCATGATCGTCTTTTCTTg                  >  1:366823/1‑134 (MQ=255)
                                                                                                                     tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        <  5:5502/91‑1 (MQ=255)
                                                                                                                     tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        >  6:5502/1‑91 (MQ=255)
                                                                                                                     tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAAttt            >  1:316951/1‑135 (MQ=255)
                                                                                                                     tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAAttt            >  8:87911/1‑135 (MQ=255)
                                                                                                                     tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAAtt             <  1:249283/134‑1 (MQ=255)
                                                                                                                     tgGAGCGATTCCAAGATTGTCGACAAGCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCCGCTGCCCTTGCCCGGTCCACATGATCTTCGTTTCTTGAAAtat            >  8:83800/1‑133 (MQ=255)
                                                                                                                        aGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATtta           <  8:235223/133‑1 (MQ=255)
                                                                                                                          cGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTAc       >  5:290872/1‑135 (MQ=255)
                                                                                                                            aTTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACtt     <  4:171675/135‑1 (MQ=255)
                                                                                                                                cAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATc  <  7:76485/134‑1 (MQ=255)
                                                                                                                                cAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATc  <  7:159661/134‑1 (MQ=255)
                                                                                                                                cAAGATTGTCGACAAGCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATTCCGTCGGGAGGCCGGACAAAGGCAGCTGACCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATc  <  1:280987/134‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
TTTCAGCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATC  >  ACB122/6101529‑6101790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑