Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,246,491 T→G 39.0% intergenic (‑84/+23) rnfA ← / ← metG Na( )‑translocating ferredoxin:NAD( )oxidoreductase complex subunit A/Methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,246,4910TG39.0% 14.6 / 5.3 41intergenic (‑84/+23)rnfA/metGNa( )‑translocating ferredoxin:NAD( )oxidoreductase complex subunit A/Methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (8/15);  new base G (13/3);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 8.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAG  >  ACB122/1246362‑1246619
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gattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGTGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATT‑TTTGTGCGCGGCAGGCC‑GCGcccc                                                                                                                              >  6:14166/1‑133 (MQ=255)
gattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCGCCca                                                                                                                              >  6:81384/1‑134 (MQ=255)
      tCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCGACCCCCATGGc                                                                                                                       >  7:259672/1‑135 (MQ=255)
         gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCAGGGC‑GCGCCCACAATTGCCCg                                                                                                                    >  7:369694/1‑135 (MQ=255)
           gcggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATGGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCGCCCACAATGGCCCGCa                                                                                                                  <  4:83505/135‑1 (MQ=255)
           gcggcgCTGAGCAGGACCAGGATGTAGGCATTCATGAGATGCCGATAGCCGGGAAGCGGGGCG‑ATTGGCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCa                                                                                                                  <  2:242428/135‑1 (MQ=255)
             ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCCGGCC‑GCTGCCACCATGGCCCGCCgg                                                                                                                >  7:221308/1‑135 (MQ=255)
             ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCCGGCC‑GCGGCCCCAAAGGCCCGCAgg                                                                                                                >  2:335405/1‑135 (MQ=255)
             ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCCGGCC‑GCGCCCCCAATGGCCCGCAgg                                                                                                                >  7:215764/1‑135 (MQ=255)
                  tGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCggg                                                                                                           >  4:343343/1‑134 (MQ=255)
                  tGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCCGGGC‑GCGACCACAATGGCCCGCAGGGCggg                                                                                                           >  6:116764/1‑134 (MQ=255)
                       aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGGGCG‑GGCCGGCC‑GCGCCCCCCAAGGCCCCCAGGGCCGTTAGtt                                                                                                      >  7:93/1‑135 (MQ=255)
                       aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACt                                                                                                       >  4:188431/1‑134 (MQ=255)
                       aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTGGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCAGGGC‑GCTCCCGCAATGGCCCGCAGGGCgggttggt                                                                                                      >  3:132245/1‑129 (MQ=255)
                          aCCAGTATGTAGTCATTCATGATATGACGATCGCCGGGAAGCGGGGCG‑ATGGTCCGGGTTTGACATGATGGGGCCTGCGTAATTGGGGGCGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGAt                                                                                                   <  1:80090/135‑1 (MQ=255)
                          aCCAGTATGTAGTCATTCATGAGATGACGATAGCCGGGAAGCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGAt                                                                                                   <  5:179884/135‑1 (MQ=255)
                                 tGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTTGGGGCC‑GGGACGCC‑GCACCCCCCAAAGCCCCCACGGGGGGTACATTATGCGctgg                                                                                            >  1:78690/1‑135 (MQ=255)
                                      tcattcatGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGGGCG‑GGGAGGCC‑GCGGCCACAATGGGCCGCAGGGCGGGTACTTGATTCgctgggctgg                                                                                       >  3:145316/1‑135 (MQ=255)
                                      tcattcatGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCCCAAATGCCCCCAGGGCGGGTACTTGATTCGctggcctgg                                                                                       >  6:68056/1‑135 (MQ=255)
                                            atGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGGGCG‑GGGAGGGC‑GCGCCCCCAAAGGCCCGCACGGGGGGTTCTTTATTCGCTGGGCAGGCTTGgc                                                                                 >  7:30727/1‑135 (MQ=255)
                                            atGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCCGGCC‑GCGCCCACAATTGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgc                                                                                 >  3:148366/1‑135 (MQ=255)
                                              gagaTGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCCGGCC‑GCTCCCACAATGGCCCGCCGGGGGGGTACTTGATGCGCTGGCCTGGCTTTgcgc                                                                               >  1:243680/1‑135 (MQ=255)
                                               agaTGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc                                                                               <  1:309729/134‑1 (MQ=255)
                                                    aCGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCGCCCACCATGGCCCGCAGGGCGGTTTATTGATGCGCTGGGCAGGCGTTGcgccgcggg                                                                         >  6:283669/1‑132 (MQ=255)
                                                       aTAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCg                                                                      >  4:44003/1‑135 (MQ=255)
                                                          cgcGGGTATCCGGGCGAATGGCCCGGGTTTCACAGCAGGGGGCCTG‑GTTAATGGGGGTCCG‑GGCACGCCGGCCCCAAAAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCg                                                                      <  8:221308/131‑1 (MQ=255)
                                                            cggtactCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGGGGGCGCG‑GGCAGGCC‑GCGCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTc                                                                 <  4:12528/129‑1 (MQ=255)
                                                             gggAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTTGGGGCC‑GGCAGGCC‑GCGCCCCCAAAGGCCCCCACGGGGGTTACTTTATGCGCTGGCCTGGGTTTGCGCCGGGTTCCCGGCTcc                                                                >  6:229659/1‑134 (MQ=255)
                                                                 aTCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCCGGCC‑GCTCCCCCCATGGCCCGCCGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGGCCGGGCTcacccg                                                            >  8:200985/1‑131 (MQ=255)
                                                                                      ttGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAg                                        <  8:369694/135‑1 (MQ=255)
                                                                                          caggAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc                                     <  7:42817/131‑1 (MQ=255)
                                                                                          cATGACGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc                                     <  3:343343/134‑1 (MQ=255)
                                                                                          cATGACGGGGCCCGCGTAATTGTGGGCGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc                                     <  3:182412/134‑1 (MQ=255)
                                                                                          cATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc                                     <  3:188431/134‑1 (MQ=255)
                                                                                               cggggCCGGCGTAATGGGGGGAGCG‑GGCAGGCC‑GCGCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa                                <  5:342916/133‑1 (MQ=255)
                                                                                               aGGGGCCTGCGTAATTGGGGGCGCG‑GGCTGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa                                <  8:267317/134‑1 (MQ=255)
                                                                                               aGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa                                <  6:348878/134‑1 (MQ=255)
                                                                                                     cggCGTATTTGGGGGCGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGa                         <  5:283669/133‑1 (MQ=255)
                                                                                                     cTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGa                         <  3:149442/135‑1 (MQ=255)
                                                                                                                             ggCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAg  <  2:42305/135‑1 (MQ=255)
                                                                                                                             ggCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCAGCAGCCATGACCATGCCTTCGGACACGCCAAAg  <  3:192570/135‑1 (MQ=255)
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GATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAG  >  ACB122/1246362‑1246619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑