Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,718,463	T→G	34.2%	Q65P (CAG→CCG)	CJ019_01614 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,718,463	0	T	G	34.2%	43.4 / 11.1	60	Q65P (CAG→CCG)	CJ019_01614	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (7/30);  new base G (14/8);  total (22/38)
Fisher's exact test for biased strand distribution p-value = 7.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGG  >  ACB122/1718335‑1718592                                                                                                                                 |                                                                                                                                  tgatgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgcg                                                                                                                             >  3:281360/1‑135 (MQ=255) tgatgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                              <  7:200792/134‑1 (MQ=255)     gaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAg                                                                                                                         <  4:305230/135‑1 (MQ=255)           cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCAc                                                                                                                   >  7:92066/1‑135 (MQ=255)           cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCggggccc                                                                                                                   >  5:283245/1‑131 (MQ=255)           cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTcgc                                                                                                                   >  1:187350/1‑133 (MQ=255)               ttGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCa                                                                                                               <  8:317357/135‑1 (MQ=255)                   cATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGGCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAgggg                                                                                                           <  3:299517/135‑1 (MQ=255)                        aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGGGCGGCAGGGGCGTcc                                                                                                      >  1:12390/1‑135 (MQ=255)                              ctGCGCGCACTACCCGCATTGCGGTGGCCAGGCTTGCCCTGCGCGGGGTCATAGGGGAGTTGGAACGGCAGCGGCACGTTGCCTTGGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGc                                                                                                <  2:65935/134‑1 (MQ=255)                              aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGGGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                <  7:296059/135‑1 (MQ=255)                              aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                <  3:373363/135‑1 (MQ=255)                              aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                <  7:202106/135‑1 (MQ=255)                                 cgcgcACTTACCGCATAGCGGTGGGCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                             <  7:24075/135‑1 (MQ=255)                                 cgcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGGGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                             <  7:79312/135‑1 (MQ=255)                                 cgcgcACTTACCGCATAGCGGTGGCCAGGCGTGACCGGCGCGGGGGCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                             <  5:86/135‑1 (MQ=255)                                 cgcgaAATTACCGCATAGGGGGGGCCCGGCTTGACCGGGGGGGGGTCATCGGGGCGAGGCACCGGCCGCGGCACGTGGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGac                                                                                             <  4:23378/135‑1 (MQ=255)                                  gcgcACTTACCTCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTTGCGCGCCAGGGTCACCGCCGGGGCGTGCAGCcgcgcc                                                                                             >  6:267052/1‑132 (MQ=255)                                  gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGCCCGGGTCCCGGCAGGGGCGGCCAACAGCgccc                                                                                            >  6:22373/1‑132 (MQ=255)                                  gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGGCCCTCAGCGcca                                                                                            >  4:153257/1‑135 (MQ=255)                                  gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCCCGGCCGGGGCGGCCCTCCGCGaca                                                                                            >  5:66975/1‑135 (MQ=255)                                   cgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGacac                                                                                           <  8:57311/135‑1 (MQ=255)                                      aCTTACCGATTAGCGGTGGCCAGGCTGGACGTGCGCGGGGTCATTGGTGACAGGGAACGGCAGCGGCAGGTTGCGTTTGACGGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATc                                                                                        <  4:327797/135‑1 (MQ=255)                                       cTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGGACGGCCGGGGCGGGCCGCAGCGACCCCTcc                                                                                       >  8:16813/1‑135 (MQ=255)                                            cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCCGGGGCGGCCCTCCGCGACCCCGCCTGCAg                                                                                  >  8:202106/1‑135 (MQ=255)                                                   cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                            <  4:332367/134‑1 (MQ=255)                                                   cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCCCGGCCGGGGCGGCCCTCCCCGCCACATCCGTCCGTTccccc                                                                           >  2:346922/1‑132 (MQ=255)                                                   cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACg                                                                           <  2:281646/135‑1 (MQ=255)                                                           aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGGCCCGGCGGGGGCGGCCCTCAGCGCCCCATCCTGCGGGGCCGCGCGCAGGGt                                                                   >  3:325256/1‑135 (MQ=255)                                                               ttGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGCCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                               <  7:130837/135‑1 (MQ=255)                                                               gtgcccTGCGGGGGGGGATGGGTGAGCTGCACCGGACGCGCCACGTGGCGGTTGCCCGGGCGGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGGCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                               <  7:86637/131‑1 (MQ=255)                                                               gtGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                               <  4:59614/134‑1 (MQ=255)                                                                tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGTCCCTCAGCGCCACCTCCTGCAGTTCCCCGCGCCGGGGggcgg                                                              >  6:77998/1‑135 (MQ=255)                                                                tGACCTGCGCGGGGTCATAGGTGAGATGCAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcgg                                                              <  5:267052/135‑1 (MQ=255)                                                                    gtGCGCGGGGTCTTAGGTGACATGTAACGCCAGCGGCAGGTTGCCTTTGACCGGGCCGTCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                          <  3:183853/134‑1 (MQ=255)                                                                    cTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                          <  8:265502/135‑1 (MQ=255)                                                                    cTGCGCGGGGGCATAGGTGAGATGGACCGGCCGCGGCACGTTGCCTGTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                          <  1:44959/135‑1 (MQ=255)                                                                     tGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGCGCGGCCGGGGCGGCCCTCAGCGCCCCCGCCTGCAGGTCCCCCCTCCGGGGggcgggcggc                                                         >  5:187422/1‑130 (MQ=255)                                                                          ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCAGGCGCGCCACGGGCACCGCCGGGGCGTCCATCCGCGACACCTCCAGCAGTTACACGCTCACGGGggcggccggcggccg                                                    >  3:107889/1‑133 (MQ=255)                                                                              tCATAGGTTAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCa                                                <  3:129386/135‑1 (MQ=255)                                                                               cATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGGGGGCGCGCCCGGGGCCCGGCCCGGGGGGGGCGCAGCGACACATCCTGCCGGTACACGCGGAGGGTGGCGGCCGGCGGCCGGGcca                                               >  8:244836/1‑133 (MQ=255)                                                                                aTAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAAt                                              <  3:184402/135‑1 (MQ=255)                                                                                    gggagaGGGAACGGCAGCGCCACGTTGCCTTTGCCCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                          <  2:187350/133‑1 (MQ=255)                                                                                    gTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGGACCGCAGGGGCGGCCCTCAGCGCCACAACCAGCCCTTCCCCGCGCAGGGTGGCGGCCCGCGGCAGGGCCATgcgc                                          >  3:262796/1‑135 (MQ=255)                                                                                    gTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                          <  7:244836/135‑1 (MQ=255)                                                                                          tGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCAGGGGCGGCCATCAGCGCCACAACCTGCACGTCCCCGCGCATGGTGGCGGCCGGCGGCCGGGCCATGCGCGGCAgg                                    >  6:249962/1‑135 (MQ=255)                                                                                                 gCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGGCCGGGGCCCGGCCGGGGGGGCCATCCGCGCCACAGCCGGCAGATCCCCGCGCCGGGGGGCGGGCGGCGGCCGGGCCATGCGCGGCAgggggcag                              >  8:59111/1‑128 (MQ=255)                                                                                                   cggggCAGGTTGCCTTTGCCGGGGCGGGCGGGGGGCGCCTGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                           <  2:180218/132‑1 (MQ=255)                                                                                                   cggggCACGTGGCCTTTGACGGGGCCGGCGTGGTGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGTCAATGCGCTGCAGGTAGAAGACt                           <  1:158365/132‑1 (MQ=255)                                                                                                   cgCGGCAGGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                           <  5:344722/134‑1 (MQ=255)                                                                                                   aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                           <  5:189187/135‑1 (MQ=255)                                                                                                   aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                           <  2:31027/135‑1 (MQ=255)                                                                                                   aGCGGCACGTTGCCGTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                           <  4:223204/135‑1 (MQ=255)                                                                                                     cGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCGCCCCAACCAGCAGTTCCACGCGCAGGGTGGCGGCCGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTc                         >  6:186783/1‑135 (MQ=255)                                                                                                                 tttGCCCGGGCCGGCTGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCt             <  5:372116/135‑1 (MQ=255)                                                                                                                    gACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTg            <  3:243589/133‑1 (MQ=255)                                                                                                                     aCCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGcc         <  7:19258/135‑1 (MQ=255)                                                                                                                      ccGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGGAGAAGACTTCACCGTCCAGGCTGGcc         <  7:19263/134‑1 (MQ=255)                                                                                                                          gccggccgGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt    <  5:34358/135‑1 (MQ=255)                                                                                                                          gccggccTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt    <  8:92066/135‑1 (MQ=255)                                                                                                                            cggccGGGCGCGCCAGGGTCACGGCCGGGGCGTCCATAAGCGACCCAACCTGGAGTTCCACGCGCCGGGTGGCGGCCGGCGGCCGGGCAATGCGCGGCCGGGTGAACACTTCCACGTCCAGGGGGGCCTGGGtgg  >  6:207347/1‑135 (MQ=255)                                                                                                                                 |                                                                                                                                  TGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGG  >  ACB122/1718335‑1718592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑