Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,194,920 T→C 6.6% F397F (TTT→TTC)  puuB_3 → Gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,194,9200TC 6.6% 186.5 / 2.2 61F397F (TTT→TTC) puuB_3Gamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (31/25);  new base C (2/2);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCGGCGGCCAAGTTGCTGG  >  ACB122/3194786‑3195047
                                                                                                                                      |                                                                                                                               
tgGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGttt                                                                                                                                 >  6:87669/1‑135 (MQ=255)
tgGAGTCGTTACCTCAATGGCGCCTAAGACTTTATCCATGTTATCGGAGCCATCGGCGCGAAACAGAACATTTACTACGCCACCGCCAGCTCGGGCCACGGGCTGGCCACGCAATCGTTGATCGCCAATCTCttt                                                                                                                                 >  6:301664/1‑135 (MQ=255)
          gcGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  3:268414/2‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  8:21611/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  3:274795/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  4:109708/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  6:193352/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  1:194156/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCaaa                                                                                                                       >  6:141661/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACAATTACTACCCCACCGGCTGCGCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTAGCCCAGCaaa                                                                                                                       >  5:7814/1‑135 (MQ=255)
          aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCCGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaa                                                                                                                        >  1:200031/1‑134 (MQ=255)
          aCGACAGTGCCGACAACGACTTTCTTCCGGTCATAGGTGCAATAGTAGACAAACAGAACATTTACTACGCCAACTGCTGATCTGTCCAAGGGCTTGGCACGCAATCGTTGATACTCAAGCTGTTTGCCCAGCaaa                                                                                                                       >  8:261544/1‑135 (MQ=255)
               aGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAGTCAAc                                                                                                                  >  7:208433/1‑135 (MQ=255)
               aGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAAc                                                                                                                  >  1:83557/1‑135 (MQ=255)
                  ggCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGc                                                                                                               >  2:137157/1‑135 (MQ=255)
                  ggCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGc                                                                                                               >  1:236884/1‑135 (MQ=255)
                    cgcCTACGACTTTCTGCCGGTGATCGGTGCAATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGCGCCACGGGCTGGGCACGCAAACGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCa                                                                                                             >  3:188641/1‑135 (MQ=255)
                    cgcCTACGACGTTATGCCCGTGATCGGTGCCATAGGCGAGAAACAGAACAATTACTACGCCACCGGCTGCTACGGCCACGGGCAGGGACAGCAATCGTTCATCGGCAAGCTGTTTGCCCAGCAAATCAAAGGCCa                                                                                                             >  8:254962/1‑135 (MQ=255)
                         aCGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACAGTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGgcgc                                                                                                        >  3:128385/1‑135 (MQ=255)
                                     cGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            <  7:21611/135‑1 (MQ=255)
                                     cGGTGATCGGTGCCAGGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAgctgc                                                                                               <  6:146705/132‑1 (MQ=255)
                                            cGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACt                                                                                     <  4:274795/135‑1 (MQ=255)
                                             ggggCCCTGGGCGCGAAACAGACCATTTCCTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTg                                                                                    <  1:152664/132‑1 (MQ=255)
                                                gcattGGGCGCGAACCAGAACTTTACCTCCGCCACCGGCTGCGCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCATCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCaa                                                                                 <  5:53955/131‑1 (MQ=255)
                                                     gggCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACaa                                                                            <  4:268414/135‑1 (MQ=255)
                                                       gcgcGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACaaaa                                                                          <  2:39242/135‑1 (MQ=255)
                                                        cgcgAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAAc                                                                         <  8:165713/135‑1 (MQ=255)
                                                            aaaCAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCg                                                                     >  1:49253/1‑135 (MQ=255)
                                                            aaaCAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            >  6:14657/1‑112 (MQ=255)
                                                            aaaCAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            <  5:14657/112‑1 (MQ=255)
                                                               cAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTcc                                                                  <  5:117318/135‑1 (MQ=255)
                                                               cAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTcc                                                                  <  4:72580/135‑1 (MQ=255)
                                                                      tttACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTtgctgc                                                           <  5:141661/135‑1 (MQ=255)
                                                                          ctacGCCACCGGCTGCTCGGGCCACTGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTg                                                        <  7:62244/134‑1 (MQ=255)
                                                                          ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            >  4:45025/1‑98 (MQ=255)
                                                                          ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            <  3:45025/98‑1 (MQ=255)
                                                                          ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTg                                                        <  4:168713/134‑1 (MQ=255)
                                                                          ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTg                                                        <  3:33709/134‑1 (MQ=255)
                                                                           tacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCTAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGaa                                                      <  2:216131/135‑1 (MQ=255)
                                                                            acGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAc                                                     >  5:298792/1‑135 (MQ=255)
                                                                              gCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAccc                                                   <  1:10475/135‑1 (MQ=255)
                                                                               ccACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGTCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCt                                                  >  8:231256/1‑135 (MQ=255)
                                                                               ccACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACCCCGTCCTTGCTGCCTGAACCCt                                                  >  8:225550/1‑135 (MQ=255)
                                                                                  ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            >  8:277021/1‑90 (MQ=255)
                                                                                  ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg                                                                                            <  7:277021/90‑1 (MQ=255)
                                                                                  ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTg                                                >  7:160944/1‑134 (MQ=255)
                                                                                  ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTg                                                >  6:145355/1‑134 (MQ=255)
                                                                                   cGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAccc                                                   >  2:338818/1‑130 (MQ=255)
                                                                                   cGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAccc                                                   <  1:338818/130‑1 (MQ=255)
                                                                                   cGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCa                                              <  8:309767/135‑1 (MQ=255)
                                                                                     gctgctCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAAt                                            <  3:339309/135‑1 (MQ=255)
                                                                                     gctgctCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAAt                                            >  6:154200/1‑135 (MQ=255)
                                                                                      ctgctCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATg                                           <  7:48282/135‑1 (MQ=255)
                                                                                            gggCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGCGCTGCTGGCATCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTccgc                                     >  2:72621/1‑135 (MQ=255)
                                                                                             ggCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTgcg                                      <  8:229020/133‑1 (MQ=255)
                                                                                                  cGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTCCCCAGAAAACCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTaca                               <  5:306013/135‑1 (MQ=255)
                                                                                                           cACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCtt                      >  2:374764/1‑135 (MQ=255)
                                                                                                           cACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCtt                      >  1:374861/1‑135 (MQ=255)
                                                                                                           cACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCtt                      >  6:62817/1‑135 (MQ=255)
                                                                                                                  tCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTgcggcg               <  5:87669/135‑1 (MQ=255)
                                                                                                                  tCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTgcggcg               >  8:98905/1‑135 (MQ=255)
                                                                                                                    gTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCggcggc             <  2:219284/135‑1 (MQ=255)
                                                                                                                               aGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCGGCGGCCAAGTTGCTgg  >  8:127938/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                               
TGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCGGCGGCCAAGTTGCTGG  >  ACB122/3194786‑3195047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑