Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,688,831 T→G 27.8% L281R (CTG→CGG)  yhaJ_2 → HTH‑type transcriptional regulator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,688,8310TG27.8% 34.4 / 2.3 46L281R (CTG→CGG) yhaJ_2HTH‑type transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base T (11/21);  new base G (5/9);  total (16/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCTG  >  ACB122/4688700‑4688948
                                                                                                                                    |                                                                                                                       
cTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCTggg                                                                                                                      >  7:37421/1‑135 (MQ=255)
     tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCCGTGGGCCGGGgggcgc                                                                                                                 >  7:81779/1‑135 (MQ=255)
     tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCGGGgcgcgc                                                                                                                 >  3:264362/1‑135 (MQ=255)
     tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCTGGgcgcgc                                                                                                                 >  7:260703/1‑135 (MQ=255)
       ccGCAGCCATGGGTCGAACCGCTGATTGCCGCGGGCGCACTGGCCGAGGTGCGCTTCGACACCATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGa                                                                                                               <  7:315424/135‑1 (MQ=255)
          cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCGGGGCGCGCGgcgc                                                                                                            >  3:267561/1‑135 (MQ=255)
          cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCCGTGGGGCGGGGCGCGcgccgc                                                                                                            >  3:272562/1‑135 (MQ=255)
          cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCACCCGTTGGGCCTGGGCGCGCGACg                                                                                                             >  6:275857/1‑134 (MQ=255)
          cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCACCCGTTGGGCCTGGGCGCGCGACg                                                                                                             >  6:271090/1‑134 (MQ=255)
           aGCCATTGGGCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGGGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGACCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCt                                                                                                           <  8:321130/135‑1 (MQ=255)
                ttGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCGTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTTGGCCGGGGGGCGCGCCGCGACCTg                                                                                                      >  1:208414/1‑135 (MQ=255)
                   gtctacccGCTGATTGCCTCGGGCGCAGTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGAAAAAGCCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGa                                                                                                    <  4:371846/128‑1 (MQ=255)
                                       gggCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCGTTGGGCCTGGGCGCCCGGCGGGACCCGGAAGTCATG‑CGCCCGCTGtt                                                                                 >  2:6707/1‑132 (MQ=255)
                                       gggCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCTCCCTGGAAGTCATG‑CGCCAGCTGtt                                                                                 >  4:57052/1‑132 (MQ=255)
                                           gcACTGGCCGCGGTGCGCTTCGAAAACATGGCCAACCGCCTGCGGTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAg                                                                          <  4:250747/135‑1 (MQ=255)
                                            cagtGGCCGTGGGGGGGTTCTACAACTTGGCAAACCGCGTGGGCTGGGGGT‑TAGACATCCTGTGTATCAGGGCGCGCCGGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAgg                                                                         <  4:267561/132‑1 (MQ=255)
                                            cACTGGCGGTGGGGCGCTTCGAAAACAGGGCCAACCGCCGGCGGTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCGGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAgg                                                                         <  6:381583/135‑1 (MQ=255)
                                            cACGGGCGGTGGTGCGCTTCGACAAC‑TGGCCCACCCGCGGGGGTTGGGGGTTATACATCTTGTGGATCACGTCGCGCCGGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAgg                                                                         <  4:272562/135‑1 (MQ=255)
                                                ggcggtggTGGGCTTCGACACCCTGGCCAACCGCATGCGCTTGGGGG‑TCGAAATCATCGGCAGCAAGGCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑cc                                                                    <  8:354196/129‑1 (MQ=255)
                                                 gtcgtggtgcggtgcGACAACCTGCCCAACCGCCGGCGCGTGTGGC‑TCGACCTCATGTGTATCTTGTCGGGCCCGTTGGGGCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑ccc                                                                   <  6:60235/130‑1 (MQ=255)
                                                    gtggTGCGCGTCCACAACATGCCCAACCGCGGGCGCTGGGGGG‑TCCACATCATCTGGATCACGGCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑cccccc                                                                <  7:177001/133‑1 (MQ=255)
                                                    gAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCGTTGGGCCTGGGCGCGCGCCGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGCAGGAG‑cccccc                                                                >  7:59537/1‑135 (MQ=255)
                                                    gAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGGCTGGGCGCGCGCCGCGACCTGGAAGTCATG‑CGCCAGCTGTCGGAGAAGGCG‑cccccc                                                                >  3:319025/1‑135 (MQ=255)
                                                           gctggaacaactGGGCCAACCGCCGGGGGTTTGGGT‑TACAAATCATCTGAACCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCt                                                         <  6:347627/129‑1 (MQ=255)
                                                               cGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCGACCTGGAAGTCCTG‑CGGCCGCGGGTCGAGAAGGCG‑CCCCCCCCGGCCGGat                                                      >  4:350036/1‑134 (MQ=255)
                                                                     cttGGCCAACCGCCGGCGCTTGTGGG‑TACACTCCATCTGGAGCACGGCGCGCCCGTTGGGCGGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  7:155266/133‑1 (MQ=255)
                                                                     ctgggCCACCCGCCGCCGCTTGGGGG‑TAGACATCTGTTGGAGAACGCCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  1:362870/132‑1 (MQ=255)
                                                                     caTGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCTTCTGGATCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  4:99023/135‑1 (MQ=255)
                                                                     caTGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCTACCGGGAAGTCATG‑CGCCAGCGGTTCGAGAAGGAG‑CCCCCCAGGGCCGGATATCCCtt                                               >  8:308382/1‑135 (MQ=255)
                                                                     caTGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  3:185870/135‑1 (MQ=255)
                                                                     caTGGCCAACCGCCTGCGCTTGTGGG‑TAGAAATCAGCTGGATCACGCCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  2:56516/135‑1 (MQ=255)
                                                                      aggcccAACCGCCTGCGCTTGTGGG‑TAGACACCATCTGGAGCAAGTCGCGCCCGTGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTg                                              <  3:116230/131‑1 (MQ=255)
                                                                             cccGCCTGCGCTTGTGGG‑TAGCCATCATCTGCATCAGGTCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGt                                       <  5:103567/134‑1 (MQ=255)
                                                                               cGCCTGCGCTTGTGGC‑TACACATCATCTGGATCAAGACGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTcc                                     <  1:248607/135‑1 (MQ=255)
                                                                                                   aCATCTTCTGTACCAAGTCGCGCCGGTTGGGCGTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  2:208414/135‑1 (MQ=255)
                                                                                                   aCATCATCTGGATCATGCGGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  2:317861/135‑1 (MQ=255)
                                                                                                   aCATCATCTGGATCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  2:77997/135‑1 (MQ=255)
                                                                                                   aCATCATCTGGATCAAGCCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  4:102677/135‑1 (MQ=255)
                                                                                                   aCATCATCTGGATCAACTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  4:161764/135‑1 (MQ=255)
                                                                                                   aCATAATCGGCATCACGCCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  8:167892/135‑1 (MQ=255)
                                                                                                     atcatcTGGATCAAGTCGCGCCCCTTGGGCCTGGGGGCCCGCCGCGACCGGGGACTGATGCCGCCA‑CTGGTGGACAAAGAGCCCCCCCCGGGCCGGTTA‑ACCGTGCCCCCGGCCCCGCCCAGCCGGCGTTCGccc                >  8:176040/1‑135 (MQ=255)
                                                                                                             gATCCAGGCGCACCCGGTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCttt        <  5:271090/135‑1 (MQ=255)
                                                                                                              aTCAAGTCGCACCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCttt        <  5:275857/134‑1 (MQ=255)
                                                                                                                   ggcgcgCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg  <  3:350036/133‑1 (MQ=255)
                                                                                                                   gccgcgCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg  <  1:118035/133‑1 (MQ=255)
                                                                                                                   gTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg  <  8:37421/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                       
CTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCTG  >  ACB122/4688700‑4688948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑