| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,123,238 | 0 | C | A | 57.7% | 5.4 / 21.1 | 27 | intergenic (‑240/+64) | gcvH_1/CJ019_01062 | Glycine cleavage system H protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (8/3); new base A (15/0); total (24/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGC > ACB122/1123115‑1123365 | gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGGTaagacggatg > 5:26186/1‑133 (MQ=255)gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGGAACGAGGTatg > 2:331198/1‑135 (MQ=255) tttACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCGGTGTATCGTTTTCGCTACGACGTAGCGaaaa < 7:29222/137‑1 (MQ=255) tACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTTGGTACGGCGGTGCGGAAAcc > 6:43054/1‑136 (MQ=255) ataaGCCTTTTTTATTGTTGGGAATGATATCGTAACCGGGCCCTCTGAGAGGAAACGGGTTTGAGAAACAAACCCTTGTCCACCGACGGCGAGGAGGCGGTGTATCGTTTCCGCAACCACGAACCTAAAACGATACa < 8:309546/135‑1 (MQ=255) gtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTTCGACGGTGCGAAAAAGATACAAGTGGTGgctgggctggg > 3:125417/1‑136 (MQ=255) gtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTAACACGTAGCGAAAACGAGAAAAGTTGTGCCTGGACTggg > 5:50481/1‑136 (MQ=255) gAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAg > 7:191639/1‑137 (MQ=255) cGTTACAGGTGACTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTCCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACCTAGCGAAAAAGATAAATGTGGTGGCTGGCCAGGCGATGTGCCAATg > 1:185556/1‑137 (MQ=255) cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGCAAACGATAAATGTGGTGGCTGGACTGGGGAGGTGCCAATGGCCtt > 7:67668/1‑137 (MQ=255) tGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATAAATGTGGTGCCGGGACTGGCGAGGTGCCAATGGCCTTCGGt > 7:237540/1‑137 (MQ=255) tctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTATCGAAAACGCGACATGTGGTGGCTGGACTGGCGAGGTGCCAATGGCCGTCGGTCAtg > 7:325145/1‑136 (MQ=255) tctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACATTTGGTGCCTGGACTGGCGAGGTGGCAATGGCCTTAGGTCAta > 4:77393/1‑136 (MQ=255) ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGAAGTAGCGAAAAAGATACATGTGGTGCCTGGACTGGCGAGTTGCCAATGGCCTTAGGTCAtat > 8:265910/1‑135 (MQ=255) ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACATGTGGTGGCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtct > 8:77165/1‑137 (MQ=255) ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTAAGACGGAGCGAAAACCATAAATTTGGTGCCTGGCCTGGGGAGGTGCCAATGGCCTTCGGTCAtct > 5:231121/1‑137 (MQ=255) aaaaGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGGAGCGCAAACGATACATGTGGTGCCTGGGCTGGCGCGGTGGCAATGGGCTTCGGTCATCTCACtttaa > 5:117512/1‑135 (MQ=255) aGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTTCGCCCTAGGGGAAACCATACATGTTGTTGCTGGGCTGGCGCGGTGGCCATGGCGGTCGGTCCTGTGACTTTgagca > 2:295884/1‑137 (MQ=255) gtgCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGTAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCCGAGAAAACGCGcc > 5:148564/1‑137 (MQ=255) gCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTac < 7:294116/38‑1 (MQ=255) gCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTac > 8:294116/1‑38 (MQ=255) aCCGACGGCGAGGAGGCTGTGTATCGTTTTAGCAACGACGTTGCGAAAACGAAACATGTTGTGGCTGGACTGGCGAGGGGCCAATGGCCTTCGGTGCTGTCACATTAAGCAGATGATCCAGCGAAAACGCGCCGgcc > 5:285121/1‑136 (MQ=255) gCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGGCTGGGCTGGCGAGGTGCCAATGGCCGTAGGTCATCGCACTTTAAGCAGATGATCCCGCGAAAACGCGCCGGCGCCTCggg > 6:284566/1‑137 (MQ=38) gCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGTAGCGAAAACGATACATGTGGGGCCGGGACTGGCGAGGTGGCCATGGGCTTGGGTCATCGCACTTTCAGCCGGTGGTCCAGCGAAACCGCGGCGGCGCCTCggg > 8:253874/1‑137 (MQ=255) gCGAGGAGGCTGTGTATCGTTTTAGCGACGCCGGAGCGAAAACGATAAATGGGGTGGCGGGGCTGGGGCGGTGGCCATGGCCTTCGGGCATGTCACTTTGCGCAGATTCTACAGCGCAAACGCGCCGGCGCCGCggg > 8:38001/1‑137 (MQ=255) gCGAGGAGGCTGTGTATCGTTTTAGCGACGACGGAGCGAAAACGATACAAGGGGTGCCTGGCCTGGCGGGGTGCCAATGGGCGTGGGTCATCTCACGTTCACCCTCTGATCCAGCGAAAACGCGCCCGCGCCGCggg > 2:90014/1‑137 (MQ=255) tATCGTTTTCGGTTCGACGTAGCGAAAACGATACATGTGGGGGCTGGACTGGCGAGGTGGCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGCTCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGagcagc > 6:338297/1‑137 (MQ=255) | GTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGC > ACB122/1123115‑1123365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |