Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,835,1670CG30.4% 51.7 / 19.3 47R171T (AGA→ACA) CJ019_02688hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (15/17);  new base G (13/1);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 3.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGA  >  ACB122/2835035‑2835299
                                                                                                                                    |                                                                                                                                    
tCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACt                                                                                                                                  >  4:149408/1‑137 (MQ=255)
 ccTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACtt                                                                                                                                 <  2:287574/137‑1 (MQ=255)
       cccGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTTGGtt                                                                                                                           >  3:68685/1‑137 (MQ=255)
        ccGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTtg                                                                                                                          <  5:5038/137‑1 (MQ=255)
        acGAGACAAAGTCTTTGCCGCCAAGCAGAGTAATCTCCGCTCGATGTCGAGGGTTGTTGATAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTATACTTTCGGTtg                                                                                                                          <  6:79701/136‑1 (MQ=255)
          gAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTtgtg                                                                                                                        <  1:5651/137‑1 (MQ=255)
              cAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTtg                                                                                                                          >  1:108449/1‑131 (MQ=255)
              cAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTtg                                                                                                                          <  2:108449/131‑1 (MQ=255)
                       tGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACa                                                                                                           <  1:203232/137‑1 (MQ=255)
                                gcagcGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGGCAGTTCGGTTGTGGGTAATCTATACAACGCTGttt                                                                                                  >  6:7147/1‑137 (MQ=255)
                                gcagcGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGttt                                                                                                  <  2:299296/137‑1 (MQ=255)
                                  agcGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACGTTAGGTTGTGGTTTATCTATACAACGCTGTTTCg                                                                                                >  8:204713/1‑137 (MQ=255)
                                    cGTAAGCTCCGCGCGATGTCGAGTGTTGTTGCTAGCCACTGATGTTAGTGCATCGGGAGTCATAGGTTTACCGTATGAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATAGAACGCTGTTTCGAt                                                                                              <  2:328064/137‑1 (MQ=255)
                                       aaGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCTTGTTCATTGGGTTGTGGTGTATCTTTACAACGCGGTTTAGATGGt                                                                                           >  5:2015/1‑137 (MQ=255)
                                               cgcgATGTCGAGTGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATATGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATg                                                                                   <  7:322486/137‑1 (MQ=255)
                                               cgcgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATg                                                                                   <  2:11266/137‑1 (MQ=255)
                                                 cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGaa                                                                                 >  5:151791/1‑137 (MQ=255)
                                                 cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTAGATGGTTGCTAATGaa                                                                                 >  3:115565/1‑137 (MQ=255)
                                                 cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTAATTTAGGTTGTTGAATATATATAAAAAGCTGTTTAGAAGGTTTCTAATGaa                                                                                 >  5:167684/1‑137 (MQ=255)
                                                  gATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCGACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGGTAATGaat                                                                                >  3:244983/1‑136 (MQ=255)
                                                                 tgCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGGTAATCTATAAAACGCTGTTTAGATGGTTGCTAATGAATCAGCAGAAGCTTAAt                                                                 >  8:49791/1‑137 (MQ=255)
                                                                   cTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTAATTTCGGTTGTGGTTAATCTATAAAAAGCTGTTTAGATGGTTTATAATAAATCAGCAGAATCTTAAttt                                                               >  1:259194/1‑136 (MQ=255)
                                                                   cTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTAGGGTGTGGGTAATATATACAACCCTGTTTCGATGGTTGCTAATTAAGCAGCAGAAGCTTCAttt                                                               >  3:303428/1‑136 (MQ=255)
                                                                   cTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGGCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTg                                                               <  8:95750/137‑1 (MQ=255)
                                                                        cACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTCGGTTGTGGATTATATATAAAAAGCTGTTTCGATGGTTGCTAATTAAGCAGCAGAAGCTTCATTGGCacc                                                          >  5:37352/1‑135 (MQ=255)
                                                                         aCTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTCGGTTGTGGTTAATCTATACAACGCTGTTTAGATGGTTGCTAATTAAGCAGCAGAAGCTTCATTGGCAgcg                                                         >  7:108840/1‑137 (MQ=255)
                                                                              tgatAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGGACTGTAGGTTGTGGTTATTATATACAACACTGTTTGGTTGGTTGCTAATACATCAGCCGAAGATTCATTTGAAgcgaaagg                                                    >  2:162910/1‑132 (MQ=255)
                                                                                atAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGGGCTTTAGGTTGGGGTTAATATATTCAAAGCTGTTTCGATGGTTGGTAATGAAGCAGCAGCAGCGTAATTGGCCGCGCATCGAc                                                  >  4:100173/1‑137 (MQ=255)
                                                                                     gCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACGTTGGGTTGTTGGTTATATATAAAAAGCTGTTTGGATTGTTTCTAATTAAGCAGCAGAAGCTTAATTGGCAGCGCATCGACTGATg                                             >  1:49915/1‑137 (MQ=255)
                                                                                       aTCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCa                                           <  6:151791/137‑1 (MQ=255)
                                                                                          gggAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACtt                                                                                                                                 >  4:37122/1‑48 (MQ=255)
                                                                                          gggAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTCGGGTGTGGATTATCTATAAAACGCTGTTTAGATGGTTGGTAATTAAGCAGCAGAAGCTTCATTTGAAGCGCATAGACTGATGCAGcc                                        >  5:270429/1‑137 (MQ=255)
                                                                                          gggAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACtt                                                                                                                                 <  3:37122/48‑1 (MQ=255)
                                                                                          gggAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATTAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGcc                                        >  5:332007/1‑137 (MQ=255)
                                                                                          gggAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGcc                                        >  2:35707/1‑137 (MQ=255)
                                                                                           ggAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTCGGTTGTGGTTTATCTATAAAACGCGGTTTAGATGGTTGGTAATGTAGCAGCAGAAGCTTCATTTGCAGCGCATCGACTGATGGAGCCa                                       >  2:298412/1‑137 (MQ=255)
                                                                                            gAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGGTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCaa                                      >  7:213465/1‑137 (MQ=255)
                                                                                              ttcttAGGTTTACCGTATCTATAAATTAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGGAGCGCATCGACTGATGCAGCCAAGc                                    <  5:188440/133‑1 (MQ=255)
                                                                                                  tAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACGTTGGGTTGTGGATAATCTATACAACCCTGTTTGGATGCTTGCTAAATAAGCAGCAGAATCTTCATTTGCAGCGCATCGACTGAATCAGCCAAGCGttt                                >  2:6784/1‑136 (MQ=255)
                                                                                                    ggTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTCGGGTGTGGATAATATATACAACGCAGTTTCGATGGTTGCTAATTAAACAGCAGAAGCTTCATTGGCAGCGCATCGACGTCTGCACCCAAGCGTTAcc                              >  8:228880/1‑137 (MQ=255)
                                                                                                    ggTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGTGTTAcc                              <  2:49915/137‑1 (MQ=255)
                                                                                                           cGTATCAAAAAAATAGACCGGTCGTCGAGTTTAGGTTGTGGATTATTTATAAAACCCGGTTTCGTTGGTTTGTAATTTATCATCAGAAGCGTCAATGTCAGCGCAGAGCCTGAGGCAGCCAAGCCTTAACACGCggg                       >  4:3867/1‑136 (MQ=255)
                                                                                                             tATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACt                     <  6:37352/137‑1 (MQ=255)
                                                                                                             tATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACt                     <  4:115565/137‑1 (MQ=255)
                                                                                                                  aaaaaaTAGACCGGTCGTGTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTAGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCCGCCAAGCGTTACCACGCGGACTTCGCt                >  6:156848/1‑137 (MQ=255)
                                                                                                                        tAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGGTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCGAAGCGTTACCACGCGGACTTCACTTTGTAg          <  6:167684/137‑1 (MQ=255)
                                                                                                                              gtgagtcTACTTTCGGTTTTGGATAATCTATACAACTCTGTTTCGATGGTTGCTAATGATGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTAGCACGCGGACTTCGCTTTGTAGCAGAAg    <  8:94283/133‑1 (MQ=255)
                                                                                                                              ggTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAg    <  8:133271/137‑1 (MQ=255)
                                                                                                                                tcgtcTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGa  <  2:283433/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
TCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGA  >  ACB122/2835035‑2835299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑