Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,273,8950CA24.0% 76.2 / 20.0 50D40E (GAC→GAA) fabG_53‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base C (21/17);  new base A (0/12);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 5.14e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGC  >  ACB122/3273766‑3274029
                                                                                                                                 |                                                                                                                                      
ggAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGc                                                                                                                                 <  3:259639/137‑1 (MQ=255)
 gAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCa                                                                                                                                >  5:230518/1‑137 (MQ=255)
  aggcaggcATGTCATAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAg                                                                                                                               <  4:246657/137‑1 (MQ=255)
       ggcaTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgct                                                                                                                          >  4:225987/1‑137 (MQ=255)
       ggcaTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgct                                                                                                                          <  2:90039/137‑1 (MQ=255)
         caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGTCCGACAAACTGCAGCCgctgc                                                                                                                        <  3:105413/137‑1 (MQ=255)
         caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctgc                                                                                                                        <  3:110589/137‑1 (MQ=255)
         caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctgc                                                                                                                        >  4:303621/1‑137 (MQ=255)
                         aaaGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACAcgc                                                                                                        >  2:295459/1‑137 (MQ=255)
                                 gCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcg                                                                                                >  4:148217/1‑137 (MQ=255)
                                   tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcggc                                                                                              >  1:316431/1‑137 (MQ=255)
                                         gcGGGGCGGGCGACGCCACTGGCGGCGAGAGCGCCAAGCGTTTGGCGCGTGAGGGCTATATTGCCTGCGAACCCCGGCGCCCGGCCGACAAACTCCAGCCGCTGCTGGAGCAAAACCACGCCGCTGGCGGCCAGGcc                                                                                        <  1:179677/136‑1 (MQ=255)
                                              gggggcgACGCCACTGGCGGCGAGATCGCAATGGGTTTTGGGCGTGAGGGCTTTATTGCCTGCTTAACCCGGCGCCAGGCCTAAAAACTGCAGCCGCTGGTGGTGTAAATACACGCCGCTGGCGGCCAGGCCTACgg                                                                                   <  6:192997/135‑1 (MQ=255)
                                              gcgggcgCCGCCAGTGGCGGCCAGAGCGACACGCGTTTTGCGCGGGCGGCCTAAATTCCCTGCTAAACCCGTCGCCAGCCCTAAAAACTGCCGCCGCTGCTGTAGAAAATCCACGCCGCTGGCGGCCAGGCCTACgg                                                                                   <  4:165254/137‑1 (MQ=255)
                                              gcgggcgACGCAACTGGCGGAGATATCGCAAAGCTTTTTGCGCTTGAGGGATATATTGCCTGCGTAACCCGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACgg                                                                                   <  7:191041/137‑1 (MQ=255)
                                                     aCGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGc                                                                            >  8:106942/1‑137 (MQ=255)
                                                        ccccttgcggcgAGATCGCCAAGCGTTTTGCGGGTGATGGCTATTTTGCCTGCGTACCCGGTCGCCTGGCCTACAAACTGCACCCGCGGCGGCAGGAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGctct                                                                         <  8:92386/131‑1 (MQ=255)
                                                          aCTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGa                                                                       >  4:261585/1‑137 (MQ=255)
                                                          aCTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGa                                                                       >  7:274979/1‑137 (MQ=255)
                                                                  ggttagcGCCATGCGTTTTGCGCGTGAGGTATTTTTTGCCTGCTAAACCCTGCGCCTGGCCTAAAAACTGCAGCCGGTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCa                                                               <  3:303621/131‑1 (MQ=255)
                                                                               ttttttGGGGGGGAGGGATATATTGGGTGCGAAACCCGTCGCCTGCCCTAAAAAATCACGGCGGTGGTGTATTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGt                                                  <  2:161750/135‑1 (MQ=255)
                                                                                 ttttGCGGGTGCGGGCTATATTGCCTGCGTACCCGGGCGCCCGGCCGAAAAACTGCCGCCGCGGGTGGAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTgg                                                <  3:146350/137‑1 (MQ=255)
                                                                                 ttttGCGGGTGAGGGATATATTGCGTGCGTAACCGGTCGCCCGGCCTAAAAACTCCCGCCGGTGGTGGCGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTgg                                                <  6:205101/137‑1 (MQ=255)
                                                                                 ttttGCGCGTGAGGGCTATATTGCCTGCTTACCCCGTCGCCAGGCCGAAAAACTGCAGCCGCTGCTGTAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTgg                                                <  5:132205/137‑1 (MQ=255)
                                                                                 ttttGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATg                                                                     >  8:311236/1‑116 (MQ=255)
                                                                                 ttttGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATg                                                                     <  7:311236/116‑1 (MQ=255)
                                                                                   ttGCTCGTTAGGGAAATATTCCCGGCTAAACCCGTCCCCAGGCAGACAAACTGCCTCCGGGGCTGGAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                              <  6:255823/137‑1 (MQ=255)
                                                                                   ttGCGCGTGAGGCCTATATTGCCTGCTAAACCCGCCGCCAGGCCGACAAACTGCCGCCGCGGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                              <  7:186479/137‑1 (MQ=255)
                                                                                   ttGCGCGGTAGGTATATATTGCCTGCGTAACCCGTTCCAAGGCCTACAAACGGCCGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                              <  2:202015/137‑1 (MQ=255)
                                                                                    tGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCg                                             >  8:310230/1‑137 (MQ=255)
                                                                                        ggggAGGGATATATTGCCTGCTAAACCCGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGAGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                                         <  6:230518/134‑1 (MQ=255)
                                                                                           ttgggCTATTTTGCCTGCGTAACCCTGCCCCAGGCCCAAAAACTGCAGCCGCTGCTGGAGTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGt                                      <  5:170141/135‑1 (MQ=255)
                                                                                               gCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgct                                                                                                                          <  5:180635/49‑1 (MQ=255)
                                                                                               gCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgct                                                                                                                          >  6:180635/1‑49 (MQ=255)
                                                                                                  attttGCCTGCGAAACCCGCCCCCAGGCCTAAAAACTGCCGCCGCTGCTGTAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt                               <  3:225987/134‑1 (MQ=255)
                                                                                                  atatTGCGGGCTAAACCCGCCGCCCGGCCCACAAACTGCAGCCGCTGGTGGAGCAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt                               <  2:102832/137‑1 (MQ=255)
                                                                                                  atatTCCCTGCGTAACCGGTCGCCAGTCATACAAACTGCAGCCGCTGGTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTTGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt                               <  1:23844/137‑1 (MQ=255)
                                                                                                     ttGCCTGCGTAACCCGTCGCCAGGCCTACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATc                            <  2:11413/137‑1 (MQ=255)
                                                                                                      tGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCg                           >  6:21715/1‑137 (MQ=255)
                                                                                                       gCCTGCGTAACCCGGCCCCCGGCCGACAAACTGCCGCCGCTGCTGGAGGAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa                          <  8:169436/137‑1 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCgg                      >  4:230226/1‑136 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt                      >  7:233890/1‑137 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt                      >  4:319195/1‑137 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt                      >  1:1630/1‑137 (MQ=255)
                                                                                                             gTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGa                    >  5:336849/1‑137 (MQ=255)
                                                                                                                aCCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGAGGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGAGTGatat                 >  5:308610/1‑137 (MQ=255)
                                                                                                                      ggCCAGGCCGAAAAACTGCCGCGGCTGGGGGTGTAATTACACGCCGCTGGCGGCGAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGACGAGGTGGCCGAAGTGGTAGAGACTATCGAGCGTGATATAGGTcc           <  7:75285/136‑1 (MQ=255)
                                                                                                                      cGCCAGGCCGACAAACTGCAGCGGCTGCGGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTcc           <  6:338813/137‑1 (MQ=255)
                                                                                                                         cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGAt        >  4:272905/1‑137 (MQ=255)
                                                                                                                               gACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGc  >  5:60799/1‑137 (MQ=255)
                                                                                                                                 |                                                                                                                                      
GGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGC  >  ACB122/3273766‑3274029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑