Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,413,5530AC26.3% 90.8 / 11.0 57I7L (ATC→CTC) CJ019_04183hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/17);  new base C (0/15);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 3.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGAATGCCCTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCCTAAGGCTA  >  ACB122/4413418‑4413688
                                                                                                                                       |                                                                                                                                       
cGAATGCCCTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGGCCGCTGGATCGGCCGGCGCATCCAACAAAAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAAt                                                                                                                                        <  2:8578/137‑1 (MQ=255)
     gCCCTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACt                                                                                                                                   >  5:283566/1‑137 (MQ=255)
     ccccTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCGGTGTGCGGCGTCCGCTGGATCGGCCGGCGCATCCAACAAAAAATTCAACGTCTTGCTTGAGGTTACCCATCCCCACATAAAACAAAATCGACt                                                                                                                                   <  1:237355/136‑1 (MQ=255)
         tGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGccc                                                                                                                               >  6:111924/1‑137 (MQ=255)
          gACGGGCCGCGCTACAAGGCGATCGGCAACAGCACGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAACTCGACTGCCCg                                                                                                                              <  6:253982/137‑1 (MQ=255)
               gcggcgcTACATGGCTATCGGCAAGAGCAAGGCGGGGTTGGTCGGCCGCTGGAGCGGCCGGTGCATCCAACAACAACTTGAACGTTTTGCTTGATGTTACCCTTGCCCACAAAAAACAAAATCGACTGCCCGGCAtt                                                                                                                         <  8:106713/134‑1 (MQ=255)
               gCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCAtt                                                                                                                         <  7:214309/137‑1 (MQ=255)
                   cgcTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGcac                                                                                                                     >  5:33696/1‑137 (MQ=255)
                    gcTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGcaca                                                                                                                    >  3:262002/1‑137 (MQ=255)
                    gcTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATACAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCTCAGAAAACAAAATCGACTGCCCGGCATTGcacc                                                                                                                    >  4:5533/1‑136 (MQ=255)
                           cgcgcTCGCCAACCTCAAGGCCGGGTGGGTGGCCCGGGTGAACTGCCGGCGCATCAAAAAAAAACTTAACCTTGTTGATTGAGGTTACCATCCCCCACAAAACAAAAACTCGACTGCCCGGCATTGCACAAGCGAAt                                                                                                             <  6:43589/136‑1 (MQ=255)
                               aTCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGAATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACACAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTc                                                                                                         <  5:196017/137‑1 (MQ=255)
                                tCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCg                                                                                                        >  6:62471/1‑137 (MQ=255)
                                   gCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCt                                                                                                     >  4:300492/1‑137 (MQ=255)
                                   gCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCt                                                                                                     >  1:93428/1‑137 (MQ=255)
                                   gCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCt                                                                                                     >  5:287469/1‑137 (MQ=255)
                                   gCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCt                                                                                                     >  2:307466/1‑137 (MQ=255)
                                       cAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTGTTGGTTGAGGTTACCCATGCCCACAGAAACAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTAccc                                                                                                 <  3:263644/137‑1 (MQ=255)
                                              gCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTTAGGTTACCCATGCCCACACAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGc                                                                                          <  2:317165/137‑1 (MQ=255)
                                                cGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGa                                                                                        >  4:284348/1‑137 (MQ=255)
                                                     tcgtcgTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGAATGCCCGGCATTTCACAAGCGAATGTTCGGCTACCCGAACGGCGATcgcg                                                                                   >  4:39979/1‑137 (MQ=255)
                                                            ccGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGACTTGCTTGAGGTTACCCATGCCCACAGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCcgcg                                                                            <  4:311837/137‑1 (MQ=255)
                                                                 ggATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACgg                                                                       >  6:184110/1‑137 (MQ=255)
                                                                  gATGGGCCGCGGCAACCAACAACACATTCACCGGCTGGGTGTAGGTTACCCATGCCCACAGAAAAAAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGt                                                                      <  6:198467/137‑1 (MQ=255)
                                                                     cggccggcGAAACAAAAAAAAACTTTAACTTCTTGCTTGAGGTTACCCAGGCCCACAGAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCg                                                                   <  7:226386/137‑1 (MQ=255)
                                                                          ggcGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGc                                                              >  4:60104/1‑137 (MQ=255)
                                                                               aTCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGtt                                                         >  8:155644/1‑137 (MQ=255)
                                                                                 ccAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTAc                                                       <  2:93428/137‑1 (MQ=255)
                                                                                        caccTTGAACGTCTTGCTTGAGGTTACCCATGCCCACATAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAcc                                                <  1:275513/134‑1 (MQ=255)
                                                                                              gAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAACGCCGaa                                          >  6:188021/1‑137 (MQ=255)
                                                                                                        cttgAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTg                                >  4:8629/1‑137 (MQ=255)
                                                                                                        cttgAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTg                                >  3:312802/1‑137 (MQ=255)
                                                                                                               ttACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCga                         >  6:56853/1‑137 (MQ=255)
                                                                                                                  cccATGCCCACACAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGAt                      <  6:191488/137‑1 (MQ=255)
                                                                                                                    cATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATgg                    >  7:150963/1‑137 (MQ=255)
                                                                                                                     aTGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGc                   >  3:21804/1‑137 (MQ=255)
                                                                                                                     aTGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGc                   >  3:184811/1‑137 (MQ=255)
                                                                                                                     aTGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGc                   >  8:255146/1‑137 (MQ=255)
                                                                                                                      tGCCCACATAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  4:214131/137‑1 (MQ=255)
                                                                                                                      tGCCCACAGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  1:1606/137‑1 (MQ=255)
                                                                                                                      tGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  4:262002/137‑1 (MQ=255)
                                                                                                                      tGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  5:256698/137‑1 (MQ=255)
                                                                                                                      tGCCCACAGAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  3:249126/137‑1 (MQ=255)
                                                                                                                      tGCCCACAGAAAAAAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCGTCCCTGGTGTCGAGATGGcc                  <  5:270304/137‑1 (MQ=255)
                                                                                                                      tGCCCACAGAAAAAAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  1:238796/137‑1 (MQ=255)
                                                                                                                      ggCCCACCCAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGcc                  <  4:208716/136‑1 (MQ=255)
                                                                                                                            ccaaaaaCAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  6:108011/134‑1 (MQ=255)
                                                                                                                            caGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  8:60277/137‑1 (MQ=255)
                                                                                                                            caGAAAACAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  3:107689/137‑1 (MQ=255)
                                                                                                                            caGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  5:111924/137‑1 (MQ=255)
                                                                                                                            caGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  3:276045/137‑1 (MQ=255)
                                                                                                                            caGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCAACGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  5:188021/137‑1 (MQ=255)
                                                                                                                            aataaaaaaaaCTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  1:307466/134‑1 (MQ=255)
                                                                                                                            aaGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  4:89774/136‑1 (MQ=255)
                                                                                                                            aaGAAAAAAAACTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAAcccc            <  5:262976/136‑1 (MQ=255)
                                                                                                                               aaaaCAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGAGTCGAGATGGCCAACCCCCct         >  5:266523/1‑137 (MQ=255)
                                                                                                                                aaaCAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCcta        >  1:144925/1‑137 (MQ=255)
                                                                                                                                    aaaaTCGCCTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCAAGCTGGTTACCGTCACCGCCGACCGCATCCCTGGTGTCGAGATGGCCAACCCCCctaaggc    >  1:311949/1‑137 (MQ=255)
                                                                                                                                      aaTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCctaaggcta  <  5:184110/137‑1 (MQ=255)
                                                                                                                                      aaTCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCctaaggcta  <  5:130849/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CGAATGCCCTGACGGGCCGCGCTACAAGGCGATCGGCAACAGCAAGGCCGTGTTCGTCGTCCGCTGGATCGGCCGGCGCATCCAACAACAACTTGAACGTCTTGCTTGAGGTTACCCATGCCCACAGAAAACAAAATCGACTGCCCGGCATTGCACAAGCGAATGTTCGGCTACCCGTACGGCGATCGCGTTCCGCGTACGGTCCGCATGCTGGTTACCGTCACCGCCGAACGCATCCCTGGTGTCGAGATGGCCAACCCCCCTAAGGCTA  >  ACB122/4413418‑4413688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑