Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,927,8340AC47.8% 4.4 / 13.6 47W194G (TGG→GGG) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base A (17/7);  new base C (9/13);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 7.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCGGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAG  >  ACB122/4927699‑4927967
                                                                                                                                       |                                                                                                                                     
gcgcgGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAg                                                                                                                                      >  8:85970/1‑137 (MQ=255)
        aGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCCCCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTgggg                                                                                                                              >  1:142226/1‑135 (MQ=255)
        aGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTgggg                                                                                                                              >  1:8728/1‑135 (MQ=255)
                 aGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGcgcgtg                                                                                                                     >  1:23969/1‑132 (MQ=255)
                      gggggtCTTGCCCATGCCCGCGGGGCCGACCATGGCGACCCCCCCACCCTCTTCGTTCGGCTCTATTTCAGGCACTTCGATCATCCGGGCCAAGTGCGCCAGCAGCATGCGCCCGGCCGGGCGCGGCTCTTCGAtct                                                                                                                <  6:293353/134‑1 (MQ=255)
                          gtCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGAGCGGCGCGATTTCCGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTcggc                                                                                                            <  6:250150/137‑1 (MQ=255)
                           tCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGGTATTCGATCTcggcg                                                                                                           >  8:115495/1‑137 (MQ=255)
                           tCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCTTCGCTCTaggcg                                                                                                           >  7:25550/1‑137 (MQ=255)
                                      cccGCCGGCCCGCCCAGGGCGCTCCCCCCCCCCTCTCCGCGCGGCTGGATTACCGGCCCTTCGCCCCGCCGGGCCAAGTGACCCCGCCGCCGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCcag                                                                                                <  4:150721/137‑1 (MQ=255)
                                        cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCTCGTGGATGTCGGGGGGCCGGTCcagcc                                                                                              >  6:3240/1‑136 (MQ=255)
                                        cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGCTTCGAGAGCGGCGGTCCGGGCccgca                                                                                              >  3:142780/1‑137 (MQ=255)
                                        cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGGCGGGCGCGGCTCTGCGCTCTCGGCGGGGAGGtaccgcc                                                                                              >  5:146896/1‑131 (MQ=255)
                                             ggCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCTGGCGGGGCGGTGGGCTCTCGGGGGGGAGGGCCagcagcgcc                                                                                         >  4:308281/1‑134 (MQ=255)
                                                 gACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGTCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                     <  2:287311/137‑1 (MQ=255)
                                                 gACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                     <  6:241944/137‑1 (MQ=255)
                                                 gACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                     <  8:78351/137‑1 (MQ=255)
                                                 gACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCCATCATCCGTGCCAAGTGAGCCAGCAGCAGGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                     <  4:203974/137‑1 (MQ=255)
                                                 caCCATGGCGACCCCCCCACCCTCTTCGAGCGCCGCGATTTCAGCCACTTCTACCAGCCGTCCCAAGGGAGCCCGCCCCAGGCGCCCGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                     <  2:313107/136‑1 (MQ=255)
                                                 caCCAGGGCGACCCCCCCACCCGCTCCGAGCGCCGCGATTCCAGGCACTGCGATCCGCCGTGCCACGTGCGCCACCAGCATGCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCggg                                                                                     <  6:318443/136‑1 (MQ=255)
                                                 caCCAGGCCGATCCCCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCTATCATCCGGCCCAAGGGAGCCAGCAGCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                     <  4:79213/136‑1 (MQ=255)
                                                    cATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCGTCGCTCTCGGGGGTCAGGTCCAGCCGCTCGCGGGccc                                                                                  >  5:250133/1‑135 (MQ=255)
                                                     aTGGCGATCCCCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCTTCGATCTCGGCGGGCAGGTCCAGCCGCTCGCGGGCGAt                                                                                 >  8:283423/1‑137 (MQ=255)
                                                          gATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGGTCTTCGATCTCGGCGGGCAGGTCCAGCCGCGCGCGGGCGGTCGGcc                                                                            >  3:223402/1‑137 (MQ=255)
                                                           agcaccccacccTCTGCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGGGAGCCAGCAGCAGGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGccc                                                                           <  6:182552/135‑1 (MQ=255)
                                                                cccacccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCAGGCGCGGGTCTTCGATATCGGCGGTGAGGTCCAGCAGCTAGCGGGCCAAAGGccccgccc                                                                      >  6:282455/1‑132 (MQ=255)
                                                                  cacccTCTTCGTGCGGCTCGATTTCAGGCACTTCCATCAGCCGTCCCAAGTCCCCCAGCAGCAGGCGCCCGGCGTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAgg                                                                    <  5:282030/137‑1 (MQ=255)
                                                                     ccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGCTATCGGCGGTCCGGTCCCGCAGCTCGCCGGCGATCGGCCCGGCCACGccc                                                                 >  1:38544/1‑136 (MQ=255)
                                                                            gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCGTCGATCTCGGCGGTCAGGTCCAGCAGCAAGCGGGCGCTAGGCCCCGCCCGGCCCAGgcgcg                                                          >  6:30300/1‑136 (MQ=255)
                                                                               gggCGCGATAGCAGGCACGACGATCACCCGTCCCACCCGACCCACCACCAAGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCa                                                       <  3:107604/136‑1 (MQ=255)
                                                                               cGGCTCTATTGCCCGCACTTCGACCATCCGGGCCAAGTGACCCAGCAGCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCa                                                       <  5:254486/137‑1 (MQ=255)
                                                                                 gCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGATCTCGGCGGTCAGGTCCACCCGCACGCCGGCGATCGGCCCCGCCCGCCCCATGCGCAGCAgg                                                     >  4:172578/1‑137 (MQ=255)
                                                                                     taTTTCCGGCACTGCGATCAGCCGTGCCAAGTGCGCCAGCCGCTGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAggcggc                                                 <  3:201403/136‑1 (MQ=255)
                                                                                      aTTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGCTGCGCTGCAGgcggcg                                                >  2:198913/1‑137 (MQ=255)
                                                                                          ccggCACTTCCTACAGCCGGCCCAAGTGACCCCCCAGCCGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCaa                                            <  2:174398/135‑1 (MQ=255)
                                                                                               cctccGATCAGCCGTGCCACGTGACCCCGCAGCATGCGCCCGGCGTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGtt                                       <  6:284377/133‑1 (MQ=255)
                                                                                                     agcatcCGTGCCAAGTGAGCCCCCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCctg                                 <  2:29176/135‑1 (MQ=255)
                                                                                                            gccccACGTGACCCAGCCGCAGGCGCCTGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGc                          <  4:162666/134‑1 (MQ=255)
                                                                                                            gTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCTTGGCTCTCGGCGGGGCGGGCCCGCAGCTCGCGGGCGATCGGCCCCGCCCGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTTGGCTGCTGCGGc                          >  4:194199/1‑137 (MQ=255)
                                                                                                                    gccgCCAGCAGCCTGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGcc                  <  4:129345/134‑1 (MQ=255)
                                                                                                                     gAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGGCCAGCAGCTCGCCGGCGATCGGCCCGGACAGGCCGAAGCGCTGCAGGCGGCCCCAAACGTTGGCCTGCTGCGGCTTGCTGccc                 >  6:149133/1‑137 (MQ=255)
                                                                                                                      aGCCAGCAGCATGCCCCCGGCCTGGCGCGGCTCTTCGATCAAGGCGGTCAGGTCCAGCAGCGCGCCGGCGATCGTCCCGGAAAGGCCCATCCGCGCCCGGCGGCCCCACAGGTTGGCCTGAGTCGGCTtgctgcacg                >  6:241312/1‑134 (MQ=255)
                                                                                                                         cagcagCATGCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa             <  1:242054/137‑1 (MQ=255)
                                                                                                                          agcagcATGCGCCAGGCCTGGCGCGGCTCTTAGATCTCGGCGGGCAGGTCCAGCCGCTCGCGGGCGATAGGCCCGGCCAGGCCGATGCGAGGCAGGCGGCGCCCAAGGTTTGCCTGCTGAGGCTTTCTGCCCTGCAg            >  8:75921/1‑137 (MQ=255)
                                                                                                                           gcagcaTGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCCGCTCGCGGGCGATCGGCCCGGCCAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCGGCGGCTTGCTGCCCTGCAGc           >  8:93425/1‑137 (MQ=255)
                                                                                                                           gcagcaTGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCCGCCCGGACCGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTACAGc           >  6:71553/1‑137 (MQ=255)
                                                                                                                            cagcaTGCGCCAGGCCTGGCGCGGCTCTTCGATGTAGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGCCAGGCCGCTGCGCTGCAGGCGGGGCCAAAGGTTGGCCTGCGGCGGCTTGCTGGCCGGCCGcg          >  7:290449/1‑136 (MQ=255)
                                                                                                                                    gcCAGGCCTGGCGCGGCTCTTCGCTCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCGGCAGCTGATTcccc  >  8:332978/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GCGCGGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAG  >  ACB122/4927699‑4927967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑