Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,384,4070CG32.6% 53.4 / 17.0 43intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (9/20);  new base G (14/0);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.09e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTC  >  ACB122/5384275‑5384538
                                                                                                                                     |                                                                                                                                   
gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCgttt                                                                                                                                 >  4:215557/1‑137 (MQ=255)
 cccGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCgtttt                                                                                                                                >  7:232225/1‑137 (MQ=255)
     gcGGGCGGCTGCGGATTAAATCCGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AACACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                            <  1:283005/136‑1 (MQ=255)
         gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGcgg                                                                                                                        >  2:8298/1‑135 (MQ=255)
                                           agacaggtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                      <  1:199267/131‑1 (MQ=255)
                                              gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                   <  7:97824/137‑1 (MQ=255)
                                              gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                   <  6:307782/137‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                 <  2:198898/137‑1 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCCAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTGTTTTTTGCGTGGAATTTGGGCGCGGCGGGTCGAAAAAACGGATTTTAccc                                                                                >  8:260284/1‑137 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCAGGTATTTTGGGGCTACGGGTCGAAAAAACGCATTTAAccc                                                                                >  8:218333/1‑137 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGTATTTTGGGGCGGGGGGGGGGAAAATCGGAttttaaac                                                                                >  7:38719/1‑132 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGAATTTTGGGGCGGGGGGGCGAAAAAAGGGATTTTAccc                                                                                >  2:81062/1‑137 (MQ=255)
                                                   aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGGCGGGAAATTGGTCGGGGGCGGTGGAAAAATAGGATTTAAcaccg                                                                              >  4:260998/1‑136 (MQ=255)
                                                   aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACCCCCTCGGGCGTCTTTTTTTGTTTCATGATTTTTGGGGCTGCGGGGGGAAAAAACCGATTTAAcacaa                                                                              >  8:174654/1‑133 (MQ=255)
                                                       aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCCCCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGTAATTGGGCGGAGCGGGGCGAAAAAACGGATTTAAACCCATTCt                                                                          >  8:161360/1‑137 (MQ=255)
                                                       aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGGCGGGAATTTGGGCGGGGGGGGTGGAAAAATACGATTTAAACCCgttgt                                                                          >  8:278427/1‑135 (MQ=255)
                                                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGGGGGGATTTTTGGGCGGGGGGTCGGAAAAACGGATTTTACACCTTTAtt                                                                         >  5:91405/1‑137 (MQ=255)
                                                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTGTTTTTTTGGCTGGAATTTTGGGGGGGCGGGTGGAAAAATAGGATTTTAACCCtgtgtt                                                                         >  8:117955/1‑132 (MQ=255)
                                                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTGTTTTTTTTGCGGGTATTTGGGGGCGGCGGGGGGAAAAATAGGATTTAAACACATTCTTGt                                                                       >  4:231688/1‑137 (MQ=255)
                                                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTGTGGCGGGTATTTTGGGGGGGGGGGTGGAAAAAGACGATTTAACACCtttggtgt                                                                       >  6:321141/1‑132 (MQ=255)
                                                             gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                    <  4:98043/137‑1 (MQ=255)
                                                               gTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCTGGGATTTTGGCGCGGCGGGTGGAAAAATCGGATTTAACCCCTTTCTTggtgtgt                                                                  >  2:199267/1‑131 (MQ=255)
                                                                tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                 <  8:231795/137‑1 (MQ=255)
                                                                 aCTGGCGGACGGGGGTTCAAATCCCACCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGAAATTTGGGGCTGCGGGTCGAAAAATAGCAGTTTCCCCCGTTCTTGTTGGGCgg                                                                >  6:234248/1‑136 (MQ=255)
                                                                  cTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGtt                                                               <  3:314021/137‑1 (MQ=255)
                                                                   tGGCGGACGTGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt                                                              <  8:18361/137‑1 (MQ=255)
                                                                    ggCGGACGGGTGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                             <  6:3855/137‑1 (MQ=255)
                                                                    ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                             <  1:201951/137‑1 (MQ=255)
                                                                    ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                             <  5:12058/137‑1 (MQ=255)
                                                                    ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                             <  4:239198/137‑1 (MQ=255)
                                                                           gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGGTGGGATTTTGGGGGGGCGGGGGGAAAAAAAGGATTTTAACCCGTTTTTGTTGTGGGGTTGCCAAAGa                                                      >  8:12659/1‑137 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCGGGCATTTTGGGGCGGCGGGTGGAAAAATCGGATTTTAACCCCTTGTTGTAGGGCGTTTGCCATAGaaa                                                    >  4:126009/1‑135 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGGGCGGCGGGGGGCTAAATCGGATTTAAAACCGGTGTTGTTGGGGGGTTACCAAACATa                                                    >  2:9262/1‑137 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTTTTGGTGGGTTTTTGGGGCGGGGGGGGGGAAAATACGATTTAAACCCCTTTGTGTTGGGGGGTTGCCAAAGAtt                                                    >  2:108461/1‑136 (MQ=255)
                                                                                  cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAAGACCCCTGGGGGGGGGTTTTTTTTTGCGGGGAATTTGGGGGGGGGGGGGGGAAAAATCGGATTTAACCCCTTTGTTGTTGGGCGTTTGCCAAAGATAatgc                                                >  6:165925/1‑137 (MQ=255)
                                                                                    aaTCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcctg                                             <  6:111485/137‑1 (MQ=255)
                                                                                      tCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcg                                           <  8:265473/137‑1 (MQ=255)
                                                                                         cgccGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCt                                        <  1:79470/135‑1 (MQ=255)
                                                                                             ggCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGTGCGCGGCGGGTGGAAAAATAGGAGTTAACCCCGGTATTGTAGGGGGGTTCCCAAAGAAAAAGGCTGCGCCGGttt                                    >  8:101832/1‑137 (MQ=255)
                                                                                               cTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                  <  4:32505/137‑1 (MQ=255)
                                                                                                       aaTCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                          <  4:17638/137‑1 (MQ=255)
                                                                                                                      caCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg            <  7:118050/136‑1 (MQ=255)
                                                                                                                                ggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTTACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc  <  1:158248/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTC  >  ACB122/5384275‑5384538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑