Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,384,4150GT34.2% 50.5 / 11.4 41intergenic (+536/‑322)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (7/20);  new base T (14/0);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 4.40e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  ACB122/5384280‑5384547
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gcGGGCGGCTGCGGATTAAATCCGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AACACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                                     <  1:283005/136‑1 (MQ=255)
    gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGcgg                                                                                                                                 >  2:8298/1‑135 (MQ=255)
                                      agacaggtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                               <  1:199267/131‑1 (MQ=255)
                                         gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                            <  7:97824/137‑1 (MQ=255)
                                         gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                            <  6:307782/137‑1 (MQ=255)
                                           agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                          <  2:198898/137‑1 (MQ=255)
                                            gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCCAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTGTTTTTTGCGTGGAATTTGGGCGCGGCGGGTCGAAAAAACGGATTTTAccc                                                                                         >  8:260284/1‑137 (MQ=255)
                                            gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCAGGTATTTTGGGGCTACGGGTCGAAAAAACGCATTTAAccc                                                                                         >  8:218333/1‑137 (MQ=255)
                                            gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGTATTTTGGGGCGGGGGGGGGGAAAATCGGAttttaaac                                                                                         >  7:38719/1‑132 (MQ=255)
                                            gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGAATTTTGGGGCGGGGGGGCGAAAAAAGGGATTTTAccc                                                                                         >  2:81062/1‑137 (MQ=255)
                                              aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGGCGGGAAATTGGTCGGGGGCGGTGGAAAAATAGGATTTAAcaccg                                                                                       >  4:260998/1‑136 (MQ=255)
                                              aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACCCCCTCGGGCGTCTTTTTTTGTTTCATGATTTTTGGGGCTGCGGGGGGAAAAAACCGATTTAAcacaa                                                                                       >  8:174654/1‑133 (MQ=255)
                                                  aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCCCCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGTAATTGGGCGGAGCGGGGCGAAAAAACGGATTTAAACCCATTCt                                                                                   >  8:161360/1‑137 (MQ=255)
                                                  aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGGCGGGAATTTGGGCGGGGGGGGTGGAAAAATACGATTTAAACCCgttgt                                                                                   >  8:278427/1‑135 (MQ=255)
                                                   cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGGGGGGATTTTTGGGCGGGGGGTCGGAAAAACGGATTTTACACCTTTAtt                                                                                  >  5:91405/1‑137 (MQ=255)
                                                   cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTGTTTTTTTGGCTGGAATTTTGGGGGGGCGGGTGGAAAAATAGGATTTTAACCCtgtgtt                                                                                  >  8:117955/1‑132 (MQ=255)
                                                     gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTGTTTTTTTTGCGGGTATTTGGGGGCGGCGGGGGGAAAAATAGGATTTAAACACATTCTTGt                                                                                >  4:231688/1‑137 (MQ=255)
                                                     gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTGTGGCGGGTATTTTGGGGGGGGGGGTGGAAAAAGACGATTTAACACCtttggtgt                                                                                >  6:321141/1‑132 (MQ=255)
                                                        gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                             <  4:98043/137‑1 (MQ=255)
                                                          gTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCTGGGATTTTGGCGCGGCGGGTGGAAAAATCGGATTTAACCCCTTTCTTggtgtgt                                                                           >  2:199267/1‑131 (MQ=255)
                                                           tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                          <  8:231795/137‑1 (MQ=255)
                                                            aCTGGCGGACGGGGGTTCAAATCCCACCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGAAATTTGGGGCTGCGGGTCGAAAAATAGCAGTTTCCCCCGTTCTTGTTGGGCgg                                                                         >  6:234248/1‑136 (MQ=255)
                                                             cTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGtt                                                                        <  3:314021/137‑1 (MQ=255)
                                                              tGGCGGACGTGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt                                                                       <  8:18361/137‑1 (MQ=255)
                                                               ggCGGACGGGTGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  6:3855/137‑1 (MQ=255)
                                                               ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  5:12058/137‑1 (MQ=255)
                                                               ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  4:239198/137‑1 (MQ=255)
                                                               ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  1:201951/137‑1 (MQ=255)
                                                                      gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGGTGGGATTTTGGGGGGGCGGGGGGAAAAAAAGGATTTTAACCCGTTTTTGTTGTGGGGTTGCCAAAGa                                                               >  8:12659/1‑137 (MQ=255)
                                                                        gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCGGGCATTTTGGGGCGGCGGGTGGAAAAATCGGATTTTAACCCCTTGTTGTAGGGCGTTTGCCATAGaaa                                                             >  4:126009/1‑135 (MQ=255)
                                                                        gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGGGCGGCGGGGGGCTAAATCGGATTTAAAACCGGTGTTGTTGGGGGGTTACCAAACATa                                                             >  2:9262/1‑137 (MQ=255)
                                                                        gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTTTTGGTGGGTTTTTGGGGCGGGGGGGGGGAAAATACGATTTAAACCCCTTTGTGTTGGGGGGTTGCCAAAGAtt                                                             >  2:108461/1‑136 (MQ=255)
                                                                             cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAAGACCCCTGGGGGGGGGTTTTTTTTTGCGGGGAATTTGGGGGGGGGGGGGGGAAAAATCGGATTTAACCCCTTTGTTGTTGGGCGTTTGCCAAAGATAatgc                                                         >  6:165925/1‑137 (MQ=255)
                                                                               aaTCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcctg                                                      <  6:111485/137‑1 (MQ=255)
                                                                                 tCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcg                                                    <  8:265473/137‑1 (MQ=255)
                                                                                    cgccGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCt                                                 <  1:79470/135‑1 (MQ=255)
                                                                                        ggCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGTGCGCGGCGGGTGGAAAAATAGGAGTTAACCCCGGTATTGTAGGGGGGTTCCCAAAGAAAAAGGCTGCGCCGGttt                                             >  8:101832/1‑137 (MQ=255)
                                                                                          cTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                           <  4:32505/137‑1 (MQ=255)
                                                                                                  aaTCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                   <  4:17638/137‑1 (MQ=255)
                                                                                                                 caCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                     <  7:118050/136‑1 (MQ=255)
                                                                                                                           ggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTTACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc           <  1:158248/137‑1 (MQ=255)
                                                                                                                                    ttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg  <  1:38014/137‑1 (MQ=255)
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TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  ACB122/5384280‑5384547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑