Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,384,4180CG38.5% 47.6 / 15.8 40intergenic (+539/‑319)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (4/20);  new base G (15/0);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.54e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  ACB122/5384284‑5384547
                                                                                                                                       |                                                                                                                                 
gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGcgg                                                                                                                                 >  2:8298/1‑135 (MQ=255)
                                  agacaggtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                               <  1:199267/131‑1 (MQ=255)
                                     gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                            <  7:97824/137‑1 (MQ=255)
                                     gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                            <  6:307782/137‑1 (MQ=255)
                                       agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                          <  2:198898/137‑1 (MQ=255)
                                        gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCCAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTGTTTTTTGCGTGGAATTTGGGCGCGGCGGGTCGAAAAAACGGATTTTAccc                                                                                         >  8:260284/1‑137 (MQ=255)
                                        gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCAGGTATTTTGGGGCTACGGGTCGAAAAAACGCATTTAAccc                                                                                         >  8:218333/1‑137 (MQ=255)
                                        gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGTATTTTGGGGCGGGGGGGGGGAAAATCGGAttttaaac                                                                                         >  7:38719/1‑132 (MQ=255)
                                        gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGAATTTTGGGGCGGGGGGGCGAAAAAAGGGATTTTAccc                                                                                         >  2:81062/1‑137 (MQ=255)
                                          aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGGCGGGAAATTGGTCGGGGGCGGTGGAAAAATAGGATTTAAcaccg                                                                                       >  4:260998/1‑136 (MQ=255)
                                          aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACCCCCTCGGGCGTCTTTTTTTGTTTCATGATTTTTGGGGCTGCGGGGGGAAAAAACCGATTTAAcacaa                                                                                       >  8:174654/1‑133 (MQ=255)
                                              aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCCCCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGTAATTGGGCGGAGCGGGGCGAAAAAACGGATTTAAACCCATTCt                                                                                   >  8:161360/1‑137 (MQ=255)
                                              aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGGCGGGAATTTGGGCGGGGGGGGTGGAAAAATACGATTTAAACCCgttgt                                                                                   >  8:278427/1‑135 (MQ=255)
                                               cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGGGGGGATTTTTGGGCGGGGGGTCGGAAAAACGGATTTTACACCTTTAtt                                                                                  >  5:91405/1‑137 (MQ=255)
                                               cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTGTTTTTTTGGCTGGAATTTTGGGGGGGCGGGTGGAAAAATAGGATTTTAACCCtgtgtt                                                                                  >  8:117955/1‑132 (MQ=255)
                                                 gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTGTTTTTTTTGCGGGTATTTGGGGGCGGCGGGGGGAAAAATAGGATTTAAACACATTCTTGt                                                                                >  4:231688/1‑137 (MQ=255)
                                                 gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTGTGGCGGGTATTTTGGGGGGGGGGGTGGAAAAAGACGATTTAACACCtttggtgt                                                                                >  6:321141/1‑132 (MQ=255)
                                                    gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                             <  4:98043/137‑1 (MQ=255)
                                                      gTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCTGGGATTTTGGCGCGGCGGGTGGAAAAATCGGATTTAACCCCTTTCTTggtgtgt                                                                           >  2:199267/1‑131 (MQ=255)
                                                       tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                          <  8:231795/137‑1 (MQ=255)
                                                        aCTGGCGGACGGGGGTTCAAATCCCACCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGAAATTTGGGGCTGCGGGTCGAAAAATAGCAGTTTCCCCCGTTCTTGTTGGGCgg                                                                         >  6:234248/1‑136 (MQ=255)
                                                         cTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGtt                                                                        <  3:314021/137‑1 (MQ=255)
                                                          tGGCGGACGTGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt                                                                       <  8:18361/137‑1 (MQ=255)
                                                           ggCGGACGGGTGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  6:3855/137‑1 (MQ=255)
                                                           ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  5:12058/137‑1 (MQ=255)
                                                           ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  4:239198/137‑1 (MQ=255)
                                                           ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                      <  1:201951/137‑1 (MQ=255)
                                                                  gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGGTGGGATTTTGGGGGGGCGGGGGGAAAAAAAGGATTTTAACCCGTTTTTGTTGTGGGGTTGCCAAAGa                                                               >  8:12659/1‑137 (MQ=255)
                                                                    gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCGGGCATTTTGGGGCGGCGGGTGGAAAAATCGGATTTTAACCCCTTGTTGTAGGGCGTTTGCCATAGaaa                                                             >  4:126009/1‑135 (MQ=255)
                                                                    gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGGGCGGCGGGGGGCTAAATCGGATTTAAAACCGGTGTTGTTGGGGGGTTACCAAACATa                                                             >  2:9262/1‑137 (MQ=255)
                                                                    gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTTTTGGTGGGTTTTTGGGGCGGGGGGGGGGAAAATACGATTTAAACCCCTTTGTGTTGGGGGGTTGCCAAAGAtt                                                             >  2:108461/1‑136 (MQ=255)
                                                                         cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAAGACCCCTGGGGGGGGGTTTTTTTTTGCGGGGAATTTGGGGGGGGGGGGGGGAAAAATCGGATTTAACCCCTTTGTTGTTGGGCGTTTGCCAAAGATAatgc                                                         >  6:165925/1‑137 (MQ=255)
                                                                           aaTCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcctg                                                      <  6:111485/137‑1 (MQ=255)
                                                                             tCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcg                                                    <  8:265473/137‑1 (MQ=255)
                                                                                cgccGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCt                                                 <  1:79470/135‑1 (MQ=255)
                                                                                    ggCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGTGCGCGGCGGGTGGAAAAATAGGAGTTAACCCCGGTATTGTAGGGGGGTTCCCAAAGAAAAAGGCTGCGCCGGttt                                             >  8:101832/1‑137 (MQ=255)
                                                                                      cTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                           <  4:32505/137‑1 (MQ=255)
                                                                                              aaTCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                   <  4:17638/137‑1 (MQ=255)
                                                                                                             caCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                     <  7:118050/136‑1 (MQ=255)
                                                                                                                       ggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTTACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc           <  1:158248/137‑1 (MQ=255)
                                                                                                                                ttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg  <  1:38014/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
GCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  ACB122/5384284‑5384547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑