Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,384,4280GT27.9% 53.7 / 10.0 43intergenic (+549/‑309)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (8/23);  new base T (12/0);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 8.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAA  >  ACB122/5384318‑5384558
                                                                                                               |                                                                                                                                  
agacaggtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                                          <  1:199267/131‑1 (MQ=255)
   gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                                       <  7:97824/137‑1 (MQ=255)
   gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                                       <  6:307782/137‑1 (MQ=255)
     agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                                     <  2:198898/137‑1 (MQ=255)
      gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCCAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTGTTTTTTGCGTGGAATTTGGGCGCGGCGGGTCGAAAAAACGGATTTTAccc                                                                                                    >  8:260284/1‑137 (MQ=255)
      gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCAGGTATTTTGGGGCTACGGGTCGAAAAAACGCATTTAAccc                                                                                                    >  8:218333/1‑137 (MQ=255)
      gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGTATTTTGGGGCGGGGGGGGGGAAAATCGGAttttaaac                                                                                                    >  7:38719/1‑132 (MQ=255)
      gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGAATTTTGGGGCGGGGGGGCGAAAAAAGGGATTTTAccc                                                                                                    >  2:81062/1‑137 (MQ=255)
        aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTTTGGCGGGAAATTGGTCGGGGGCGGTGGAAAAATAGGATTTAAcaccg                                                                                                  >  4:260998/1‑136 (MQ=255)
        aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACCCCCTCGGGCGTCTTTTTTTGTTTCATGATTTTTGGGGCTGCGGGGGGAAAAAACCGATTTAAcacaa                                                                                                  >  8:174654/1‑133 (MQ=255)
            aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCCCCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGTAATTGGGCGGAGCGGGGCGAAAAAACGGATTTAAACCCATTCt                                                                                              >  8:161360/1‑137 (MQ=255)
            aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGGCGGGAATTTGGGCGGGGGGGGTGGAAAAATACGATTTAAACCCgttgt                                                                                              >  8:278427/1‑135 (MQ=255)
             cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTTTGGGGGGGATTTTTGGGCGGGGGGTCGGAAAAACGGATTTTACACCTTTAtt                                                                                             >  5:91405/1‑137 (MQ=255)
             cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTGTTTTTTTGGCTGGAATTTTGGGGGGGCGGGTGGAAAAATAGGATTTTAACCCtgtgtt                                                                                             >  8:117955/1‑132 (MQ=255)
               gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTGTTTTTTTTGCGGGTATTTGGGGGCGGCGGGGGGAAAAATAGGATTTAAACACATTCTTGt                                                                                           >  4:231688/1‑137 (MQ=255)
               gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTGTGGCGGGTATTTTGGGGGGGGGGGTGGAAAAAGACGATTTAACACCtttggtgt                                                                                           >  6:321141/1‑132 (MQ=255)
                  gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                                        <  4:98043/137‑1 (MQ=255)
                    gTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCTGGGATTTTGGCGCGGCGGGTGGAAAAATCGGATTTAACCCCTTTCTTggtgtgt                                                                                      >  2:199267/1‑131 (MQ=255)
                     tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                                     <  8:231795/137‑1 (MQ=255)
                      aCTGGCGGACGGGGGTTCAAATCCCACCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCGTTTTTTGTGCCTGGAAATTTGGGGCTGCGGGTCGAAAAATAGCAGTTTCCCCCGTTCTTGTTGGGCgg                                                                                    >  6:234248/1‑136 (MQ=255)
                       cTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGtt                                                                                   <  3:314021/137‑1 (MQ=255)
                        tGGCGGACGTGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt                                                                                  <  8:18361/137‑1 (MQ=255)
                         ggCGGACGGGTGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                                 <  6:3855/137‑1 (MQ=255)
                         ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                                 <  5:12058/137‑1 (MQ=255)
                         ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                                 <  4:239198/137‑1 (MQ=255)
                         ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                                 <  1:201951/137‑1 (MQ=255)
                                gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGGTGGGATTTTGGGGGGGCGGGGGGAAAAAAAGGATTTTAACCCGTTTTTGTTGTGGGGTTGCCAAAGa                                                                          >  8:12659/1‑137 (MQ=255)
                                  gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGTTTTTTTTTGGCGGGCATTTTGGGGCGGCGGGTGGAAAAATCGGATTTTAACCCCTTGTTGTAGGGCGTTTGCCATAGaaa                                                                        >  4:126009/1‑135 (MQ=255)
                                  gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGGGCGGCGGGGGGCTAAATCGGATTTAAAACCGGTGTTGTTGGGGGGTTACCAAACATa                                                                        >  2:9262/1‑137 (MQ=255)
                                  gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGGTTTTTTTTGGTGGGTTTTTGGGGCGGGGGGGGGGAAAATACGATTTAAACCCCTTTGTGTTGGGGGGTTGCCAAAGAtt                                                                        >  2:108461/1‑136 (MQ=255)
                                       cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAAGACCCCTGGGGGGGGGTTTTTTTTTGCGGGGAATTTGGGGGGGGGGGGGGGAAAAATCGGATTTAACCCCTTTGTTGTTGGGCGTTTGCCAAAGATAatgc                                                                    >  6:165925/1‑137 (MQ=255)
                                         aaTCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcctg                                                                 <  6:111485/137‑1 (MQ=255)
                                           tCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcg                                                               <  8:265473/137‑1 (MQ=255)
                                              cgccGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCt                                                            <  1:79470/135‑1 (MQ=255)
                                                  ggCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGTGCGCGGCGGGTGGAAAAATAGGAGTTAACCCCGGTATTGTAGGGGGGTTCCCAAAGAAAAAGGCTGCGCCGGttt                                                        >  8:101832/1‑137 (MQ=255)
                                                    cTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                                      <  4:32505/137‑1 (MQ=255)
                                                            aaTCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                              <  4:17638/137‑1 (MQ=255)
                                                                           caCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                                <  7:118050/136‑1 (MQ=255)
                                                                                     ggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTTACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc                      <  1:158248/137‑1 (MQ=255)
                                                                                              ttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg             <  1:38014/137‑1 (MQ=255)
                                                                                                         gAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCaa  <  7:12659/137‑1 (MQ=255)
                                                                                                         gAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCaa  <  4:81326/137‑1 (MQ=255)
                                                                                                         gAATTTGGGCGCTGCGGGTCGAAAAAGCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCaa  <  4:219502/137‑1 (MQ=255)
                                                                                                               |                                                                                                                                  
CATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAG‑AAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAA  >  ACB122/5384318‑5384558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑