Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 1,806,746 G→C W15C (TGG→TGC)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,806,7460GC98.5% 241.0 / ‑3.9 66W15C (TGG→TGC) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (39/26);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

CACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTG  >  ACB122/1806612‑1806871
                                                                                                                                      |                                                                                                                             
cacGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCca                                                                                                                             <  6:145865/137‑1 (MQ=255)
    aTGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAg                                                                                                                         >  1:88187/1‑137 (MQ=255)
          cTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTAc                                                                                                                   >  7:90097/1‑137 (MQ=255)
            ggAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                 <  1:48081/137‑1 (MQ=255)
             gAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCgg                                                                                                                >  3:177768/1‑137 (MQ=255)
              aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGc                                                                                                               >  7:119661/1‑137 (MQ=255)
              aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGc                                                                                                               >  1:231146/1‑137 (MQ=255)
              aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGc                                                                                                               >  3:4321/1‑137 (MQ=255)
                gACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGcat                                                                                                             >  6:119757/1‑137 (MQ=255)
                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                 <  6:79232/127‑1 (MQ=255)
                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                 >  5:79232/1‑127 (MQ=255)
                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTcc                                                                                                       >  3:256837/1‑137 (MQ=255)
                      aacaaGGCCGTCGACCTGATGCAGGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCCGGATAACATCTCCTGACAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTcc                                                                                                       <  2:200927/137‑1 (MQ=255)
                          aGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAgg                                                                                                   >  8:19172/1‑137 (MQ=255)
                              cctCGTCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGTGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGCCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                               <  1:298198/135‑1 (MQ=255)
                                   aCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGtt                                                                                          >  3:232881/1‑137 (MQ=255)
                                   aCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGtt                                                                                          >  2:118542/1‑137 (MQ=255)
                                         tGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGata                                                                                    <  8:90097/137‑1 (MQ=255)
                                          gCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGatat                                                                                   >  4:301153/1‑137 (MQ=255)
                                          gCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGatat                                                                                   >  2:110015/1‑137 (MQ=255)
                                               aaGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgtgt                                                                              <  3:62044/137‑1 (MQ=255)
                                               aaGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgtgt                                                                              <  3:67126/137‑1 (MQ=255)
                                               aaGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgtgt                                                                              <  2:203344/137‑1 (MQ=255)
                                                aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGtt                                                                             >  7:106246/1‑137 (MQ=255)
                                                aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGtt                                                                             >  2:154354/1‑137 (MQ=255)
                                                 ggCAAGAGCCTCCGTAGCGTGATCCACTTCTGAGGGCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                            <  2:167295/137‑1 (MQ=255)
                                                 ggCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                            <  1:21548/137‑1 (MQ=255)
                                                 ggCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                            >  2:70459/1‑137 (MQ=255)
                                                 ggCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                            <  4:4321/137‑1 (MQ=255)
                                                   cAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGc                                                                          >  8:217559/1‑137 (MQ=255)
                                                    aaGAGCATCCGTAGCGTGATCAACTTCTGATGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGATGCCACAAGCGTTACCGGCATCATTCCAAGGTGATGGGTTGCGATATGGTGTTCGCt                                                                         >  7:242362/1‑137 (MQ=255)
                                                          atgcggaGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCCGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTAc                                                                   <  8:153472/131‑1 (MQ=255)
                                                          atgcGTATCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGACGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCGGGGTTGCGATATGGTGTTCGCTGTGTAc                                                                   <  8:242362/134‑1 (MQ=255)
                                                          aTCCGTAGCGGGATCCACTTCTGAGGTCTGTCCTGAGCCTGGATAACATCTCCTGCCATAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTAc                                                                   <  8:120762/137‑1 (MQ=255)
                                                               tAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgcc                                                              >  1:195104/1‑137 (MQ=255)
                                                                 gCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgccgc                                                            <  8:119661/137‑1 (MQ=255)
                                                                         cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGcc                                                    >  6:303896/1‑137 (MQ=255)
                                                                         cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGcc                                                    >  8:25146/1‑137 (MQ=255)
                                                                         cACTTCTGAGGGATGTCCTCAGCCTGGATAACCTCTCCTGCCAGAAGAGCTTCGGCGGCTTCCACAAGCGTTACCGGCTTCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGcc                                                    <  8:212765/137‑1 (MQ=255)
                                                                          aCTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGccc                                                   >  2:164202/1‑136 (MQ=255)
                                                                                gAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                               <  2:276900/87‑1 (MQ=255)
                                                                                gAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                               >  1:276900/1‑87 (MQ=255)
                                                                                    tCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGa                                         >  6:310262/1‑137 (MQ=255)
                                                                                    tCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGa                                         >  2:226998/1‑137 (MQ=255)
                                                                                    tCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGa                                         >  7:333826/1‑137 (MQ=255)
                                                                                      tGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaa                                       >  6:176218/1‑137 (MQ=255)
                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                    >  8:210284/1‑137 (MQ=255)
                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                    <  2:88187/137‑1 (MQ=255)
                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                    <  1:259313/137‑1 (MQ=255)
                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                    >  1:249206/1‑137 (MQ=255)
                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGAGATCGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                    >  6:190229/1‑137 (MQ=255)
                                                                                              gCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                               <  4:298165/137‑1 (MQ=255)
                                                                                              gCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                               <  7:210284/137‑1 (MQ=255)
                                                                                              gCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                               <  7:217559/137‑1 (MQ=255)
                                                                                              gCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                               <  3:27512/137‑1 (MQ=255)
                                                                                                   gATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAATCTGCCGGTGCt                          <  1:118542/137‑1 (MQ=255)
                                                                                                     tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTgg                        >  5:278326/1‑136 (MQ=255)
                                                                                                     tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGt                        >  6:170566/1‑137 (MQ=255)
                                                                                                     tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGt                        >  2:142253/1‑137 (MQ=255)
                                                                                                                gCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc             >  2:175475/1‑137 (MQ=255)
                                                                                                                gCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc             >  3:161545/1‑137 (MQ=255)
                                                                                                                gCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc             <  2:74903/137‑1 (MQ=255)
                                                                                                                      agagCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTc       >  2:322284/1‑137 (MQ=255)
                                                                                                                      agagCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTc       >  3:59670/1‑137 (MQ=255)
                                                                                                                           ttCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTg  >  6:75727/1‑137 (MQ=255)
                                                                                                                           ttCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTg  >  6:53498/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                             
CACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTG  >  ACB122/1806612‑1806871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑