Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 690,144 A→G 100% intergenic (+21/‑2438) bauC → / → PP_RS08750 Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122690,1440AG87.5% 11.5 / ‑3.2 8intergenic (+21/‑2438)bauC/PP_RS08750Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (7/0);  total (8/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

GCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTG‑GGGGTTGGTTG  >  ACB122/690020‑690184
                                                                                                                             |                                         
gCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCgggggg                                 >  5:239634/1‑130 (MQ=255)
        ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCCGGGCGccccc                         >  7:461196/1‑134 (MQ=255)
                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCTCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGTCCCGCCGCCCCCCGGTGTTTTTG‑GTgtttggtt   >  3:383545/1‑135 (MQ=255)
                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCCGGCCCCCCCCCGTGTTTTTTG‑TGggtgggtt   >  8:305043/1‑135 (MQ=255)
                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCGGCCGCCCCCGCGGTGTTTTTG‑Ggggtgggtg   >  4:73771/1‑134 (MQ=255)
                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGC‑GGCCGCCCCCCGGGGTTTTTGTGGggtgggtt   >  8:290434/1‑135 (MQ=255)
                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGCGCGGCGCCCCCCCGGGGTTTTTG‑GGggtggggt   >  2:401561/1‑133 (MQ=255)
                              gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCTTCCCCGCTTTGTTTTTG‑GGGgttggttg  >  2:319766/1‑135 (MQ=255)
                                                                                                                             |                                         
GCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTG‑GGGGTTGGTTG  >  ACB122/690020‑690184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑