Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,528,308 A→C 24.7% P87P (CCA→CCC argJ → Arginine biosynthesis bifunctional protein ArgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,528,3080AC24.7% 72.5 / 6.9 73P87P (CCA→CCCargJArginine biosynthesis bifunctional protein ArgJ
Reads supporting (aligned to +/- strand):  ref base A (42/11);  new base C (16/4);  total (58/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGC  >  ACB122/1528177‑1528438
                                                                                                                                   |                                                                                                                                  
aGCGTGGCTGGCGTGTTCACCCTCCACGCCTTCTGCGCGGCGCCGGTGCTCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTACCGCCAACGCCGGCCCCGGCGCGCCAGGc                                                                                                                                 >  2:302506/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGCCCAACACGGGTAACGCCAACGCCGGCCCCGGCGCGCCCGGc                                                                                                                                 >  7:258744/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGc                                                                                                                                 >  6:262428/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGc                                                                                                                                 >  7:48095/1‑135 (MQ=255)
  cGTGGCTGGCGTGTTCCCCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTCCCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGcca                                                                                                                               >  4:432936/1‑134 (MQ=255)
  agTGGCTGGCGTGTTCACAATCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTAAAGGTCACCGAGCGCTACCTGCTGAACAACACGGGTAACGCCAACGCCGGCACCGGCGCGACAGGCCt                                                                                                                               >  6:401316/2‑135 (MQ=255)
         ggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGgccgcc                                                                                                                        >  8:41286/1‑135 (MQ=255)
          gcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGCTCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCGGgccgccg                                                                                                                       >  3:81376/1‑135 (MQ=255)
                 cACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAgcg                                                                                                                <  6:350548/135‑1 (MQ=255)
                    ccTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCgcgccccc                                                                                                             >  6:415374/1‑131 (MQ=255)
                       cAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCCCCGACCGccccag                                                                                                          >  7:346362/1‑133 (MQ=255)
                          cGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGCCCGCCGCCGCGCGCACCTgcgc                                                                                                       >  6:346331/1‑135 (MQ=255)
                           gCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCGGGCCGCCGCCGCGCGCCCCTGCGcc                                                                                                      >  3:271995/1‑135 (MQ=255)
                            ccTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCAGCGccc                                                                                                     >  6:15966/1‑134 (MQ=255)
                            ccTTCTGCGCGGCGCCGGTGATCCTCTCCAAACAGCGTGTACAGGGCACCGTGCGCTCACTGCTGACCAACACGTGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGccc                                                                                                     >  1:334502/1‑134 (MQ=255)
                                    gcggcgCCGGTGACCCTGTCCAAACAGCGGGTACCGGCCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGcc                                                                                             <  8:137746/135‑1 (MQ=255)
                                        cgcCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCCGCCTGGCCGCCGCCGCGCGCCCCAGCGCCAACCTGGCCgccc                                                                                         >  5:334648/1‑132 (MQ=255)
                                            ggTGACCCTGTCCAACCCGCGTGTACCGGGCACCGTGCGCTACCTGCTGACCAACACGGGAAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAActggc                                                                                     <  2:265807/135‑1 (MQ=255)
                                                aTCCTGGCCACACCGCGTGAACCGGGCCCCGGGCGCAACCTGCTCACCAACCCGGGCACCGCCAACCCCGCCACCGGCGCGCCCGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACtggctggc                                                                                 <  7:292838/135‑1 (MQ=255)
                                                     gTCCAAACCGGGTGTACTGGGCAGGGTGGGAAACCTGGTCCCCAACAGGGGTACGGCCACGGCCGCCACGGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                            <  6:64728/135‑1 (MQ=255)
                                                     gTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCCAGCTGGCCGACCGGGCTGGCGTAcc                                                                            >  8:424798/1‑135 (MQ=255)
                                                     gTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                            >  3:41069/1‑135 (MQ=255)
                                                     gTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCAGACAAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCAGGACGCCGCCGAGCGCCCCTGCGCCAAGCAGGCCGAACTGGCAGGCGTAcc                                                                            >  4:263451/1‑135 (MQ=255)
                                                        cAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGCACGGGCTGGCGTACCGGc                                                                         >  1:392631/1‑135 (MQ=255)
                                                        cAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCCCCCAGCGCACCTGCGCCAAGCTGGCCGAACATGCTGGCGTACCGGc                                                                         >  1:307074/1‑135 (MQ=255)
                                                         aaaCAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCCAGCAGGCCGAACAGGCTGGCGTACCGGcc                                                                        >  5:306469/1‑135 (MQ=255)
                                                          aaCCGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCg                                                                       <  8:138892/135‑1 (MQ=255)
                                                          aaCAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCCGc                                                                         >  7:98567/1‑133 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACAGGCTGGCGTACCGGCCGAGTCGGTa                                                               >  6:443439/1‑135 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTa                                                               >  6:3506/1‑135 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCGGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTAGGtt                                                               >  6:212937/1‑134 (MQ=255)
                                                                   tACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGACCCCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTAc                                                              >  3:8724/1‑135 (MQ=255)
                                                                    aCAGGGCACCGGGCGCTACCTGCTCACCACCACGTTAAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACGGCCGAGTCGGTACTg                                                            <  2:287767/135‑1 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTTCTGCCggt                                                       >  5:98137/1‑133 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGtt                                                       >  8:25966/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAACCTGGCCGCACTGGCTGGCGTACCGGCCGCGGCGGGTCTGCCgct                                                       >  2:203569/1‑133 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGtt                                                       >  7:227559/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCGGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTTCCGGCCGCGTCGGGACTGCCg                                                         >  3:266040/1‑133 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCGTGCCCAACTGGCTGGCGTACCGGCCGAGTAGGTACTGCCGtt                                                       >  8:168945/1‑135 (MQ=255)
                                                                           aCCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCCCACCGGCGCCCAGCTGGCCGAACTGGCTGGCGTTCCGGCCGAGTCGGTACTGCCGtta                                                      >  6:202290/1‑134 (MQ=255)
                                                                              gTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTcc                                                   >  5:78410/1‑135 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAACCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTTCTGCCCGTCTCCAc                                                 >  1:283123/1‑135 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGATCTGGCTGGCCTACCGGCCGAGTCGGTACTGCCGTTCTcccc                                                 >  3:358851/1‑133 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGGTCTCCAc                                                 >  3:310514/1‑135 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCACGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTTCCGTTCTCCAc                                                 >  1:349068/1‑135 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCg                                               >  4:134102/1‑135 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCg                                               >  1:94692/1‑135 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCg                                               >  4:225000/1‑135 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCAAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTCCGCCAAGCATGCCGAACAGGCTGCCGTACCGGCCGAGACGGTACTGCCGTTCTGCAccc                                               >  8:169577/1‑134 (MQ=255)
                                                                                   cTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGGGGGTACTGCCGGTAGCCAcccg                                              >  1:182547/1‑133 (MQ=255)
                                                                                     aCCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGAGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCg                                            >  5:228118/1‑135 (MQ=255)
                                                                                                     gggTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGGACTGCCGTTCTCCCCCGGCGGGCTTGGCGGGCCGcg                            >  2:265349/1‑134 (MQ=255)
                                                                                                     gggTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCCAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACAGCCCGTCTCCCCCGGCGTGAATGGCGAGccccg                            >  2:265342/1‑132 (MQ=255)
                                                                                                     gggTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCt                            >  7:302291/1‑135 (MQ=255)
                                                                                                          acgccaacgccGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGTGGAGCCGCGTCCgg                       >  2:143923/1‑135 (MQ=255)
                                                                                                          acgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCCGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGAATTGCGCGCCGCAGCCgg                       >  1:126794/1‑135 (MQ=255)
                                                                                                          acgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGGTTGGCGCGCCGCTGCCgg                       >  3:380242/1‑135 (MQ=255)
                                                                                                           cgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTACACCGGCGTGATTGTCGAGCCGCTGCCggg                      >  2:297573/1‑134 (MQ=255)
                                                                                                            ccccccgccGGCCCCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTc                     <  3:366646/130‑1 (MQ=255)
                                                                                                                    cGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGCACGGGCTGGCGTACCGGCCGCGTCGGGACTGCCGTTCTCCACCGGCGTGATTGGGGCGGCGCGGCCGGTCGCAAAGAt             >  7:60469/1‑135 (MQ=255)
                                                                                                                    cGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGAt             >  8:315510/1‑135 (MQ=255)
                                                                                                                    cGGCACCGGCGCGCCAGGCCTGGCCCCCGCCGCGCGCAACTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGGCGGTACTGCCCGTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAACAt             >  7:69965/1‑135 (MQ=255)
                                                                                                                         cgggCGCTCCCGGCCTGGCCGGCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg        <  5:401316/133‑1 (MQ=255)
                                                                                                                         cgcccgcccCCGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg        <  5:312953/131‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCTGGTCGAAAAGATCGAAg        <  5:415374/135‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg        <  8:227559/135‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg        <  1:410562/135‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg        <  6:359102/135‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg        <  5:122703/135‑1 (MQ=255)
                                                                                                                               cgcCCGGCCTGGCCGCCGCCGAGCGCAACTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGCGTCGGTTCTGCCGTTCTCCACCGGCGTGGTTGGCGAGCCGCGGCCGGTCGAAAAGATCGAAGgcgcgc  >  4:173523/1‑135 (MQ=255)
                                                                                                                               cgcCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGGTCTCCACCCGCGGGGTTGGGGGGCCCCGGGCGGGGGGAAAAATAGAACgcgcgc  >  6:439152/1‑135 (MQ=255)
                                                                                                                               cgcCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGgcgcgc  >  2:269272/1‑135 (MQ=255)
                                                                                                                               cgcCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGGGATTGGCGCGCCGCTGCCGGTCGAAAACATCGACGgcgccc  >  8:83564/1‑133 (MQ=255)
                                                                                                                                   |                                                                                                                                  
AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGC  >  ACB122/1528177‑1528438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: