Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 169,792 G→A 7.3% intergenic (+756/+2931) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122169,7920GA 7.3% 345.4 / 12.4 117intergenic (+756/+2931)PP_RS08750/fhuA_1tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
Reads supporting (aligned to +/- strand):  ref base G (56/52);  new base A (4/4);  total (61/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

AAACTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTT  >  ACB122/169659‑169923
                                                                                                                                     |                                                                                                                                   
aaaCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGc                                                                                                                                    >  6:343294/1‑135 (MQ=32)
  aCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGCACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                                                                                                                  >  6:296223/1‑135 (MQ=17)
     ttGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTa                                                                                                                                 >  6:375118/1‑133 (MQ=32)
        tCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGGGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAg                                                                                                                             <  2:88008/134‑1 (MQ=17)
         cTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGCCTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCa                                                                                                                           <  7:284045/135‑1 (MQ=17)
           gAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAACCCGTGTACGTACAAGCAGt                                                                                                                         >  2:95298/1‑135 (MQ=11)
           gAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTCCGTACAAGCAGt                                                                                                                         >  8:278115/1‑135 (MQ=255)
              tatGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTggg                                                                                                                      <  4:229596/135‑1 (MQ=32)
              tatGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTggg                                                                                                                      <  3:365571/135‑1 (MQ=32)
                   ggggACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGTGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                                  <  4:286601/134‑1 (MQ=17)
                     ggACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTAc                                                                                                               >  8:118119/1‑135 (MQ=32)
                         cATCCTCCAAGGCTAAATACTACTGACTTACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCAGTAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGCGAGCCTACTTTg                                                                                                           >  8:44008/1‑135 (MQ=255)
                              tccAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAgg                                                                                                      >  5:260981/1‑135 (MQ=32)
                                   ggCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt                                                                                                 <  1:118248/135‑1 (MQ=32)
                                   ggCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt                                                                                                 <  1:184432/135‑1 (MQ=32)
                                    gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                                <  4:305706/135‑1 (MQ=32)
                                    gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                                <  7:242156/135‑1 (MQ=32)
                                    gCTAAATACTACTGACTGACCGATAGTGAACCAGGACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                                <  6:302629/135‑1 (MQ=17)
                                      tAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGc                                                                                               >  7:286976/1‑134 (MQ=32)
                                         aTACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                           <  2:368717/135‑1 (MQ=32)
                                          tactacTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAcc                                                                                          >  3:387454/1‑135 (MQ=32)
                                           actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCt                                                                                         >  7:389364/1‑135 (MQ=32)
                                                tgactgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGt                                                                                    >  6:284311/1‑135 (MQ=32)
                                                 gactgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGt                                                                                    >  7:402605/1‑134 (MQ=32)
                                                  actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAcc                                                                                          <  1:52637/127‑1 (MQ=32)
                                                  actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                                   >  5:333282/1‑134 (MQ=32)
                                                  actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                                   >  2:451351/1‑134 (MQ=32)
                                                   ctgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGtat                                                                                  <  1:266621/134‑1 (MQ=11)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGCCTGCGTACCTTTTGTATAAt                                                                               >  6:311291/1‑135 (MQ=17)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                               >  4:39905/1‑135 (MQ=32)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                               >  7:376999/1‑135 (MQ=32)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                               >  5:92463/1‑135 (MQ=32)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                               >  5:373217/1‑135 (MQ=32)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                               >  3:391053/1‑135 (MQ=32)
                                                     gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTCGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                               >  7:286031/1‑135 (MQ=17)
                                                       ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                                                                                                                  <  7:380838/82‑1 (MQ=33)
                                                       ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                                                                                                                  >  8:380838/1‑82 (MQ=33)
                                                       ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCa                                                                                                                           >  6:342690/1‑89 (MQ=33)
                                                       ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCa                                                                                                                           <  5:342690/89‑1 (MQ=33)
                                                       ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATgg                                                                             >  2:318863/1‑135 (MQ=32)
                                                        aaaTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATggg                                                                            <  7:337594/133‑1 (MQ=17)
                                                         gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATggg                                                                            >  6:47927/1‑134 (MQ=32)
                                                          atcgTGAACCAGTACCGTGAGGGAAAGGCGAACAGAACCGCGGCGAGGTGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  5:6342/132‑1 (MQ=11)
                                                          aTAGTGCACCAGTACCGTTAGCGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  1:324465/135‑1 (MQ=12)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  7:278115/135‑1 (MQ=11)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAc                                                                                                                                >  4:201198/1‑81 (MQ=33)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAc                                                                                                                                <  3:201198/81‑1 (MQ=33)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  6:333282/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  7:392728/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  1:146049/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  1:226685/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  3:343405/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  1:300520/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  4:387454/135‑1 (MQ=32)
                                                          aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  4:38790/135‑1 (MQ=32)
                                                                   cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACtt                                                                  >  2:221693/1‑134 (MQ=32)
                                                                   cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTCAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACtt                                                                  >  3:99002/1‑134 (MQ=17)
                                                                   cAGTACCGTGAGGGAAAGGCGAAAAGAACACCGGAGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGAGGGAGCACGCTGAGGCGTGAGACTGCGTACCTTTTGAATAATTGGTCAGCGACtt                                                                  >  5:276185/1‑134 (MQ=11)
                                                                     gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata                                                               >  2:439429/1‑135 (MQ=32)
                                                                        ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                              >  8:319822/1‑133 (MQ=32)
                                                                        ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                              >  1:440578/1‑133 (MQ=32)
                                                                         cGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCa                                                           <  4:83902/135‑1 (MQ=32)
                                                                           tGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          >  7:135261/1‑118 (MQ=32)
                                                                           tGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  8:135261/118‑1 (MQ=32)
                                                                           tGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                         >  6:274504/1‑135 (MQ=32)
                                                                              gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  7:66992/115‑1 (MQ=32)
                                                                              gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          >  8:66992/1‑115 (MQ=32)
                                                                              gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTgg                                                       >  4:205233/1‑134 (MQ=32)
                                                                              gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                      <  7:72877/135‑1 (MQ=32)
                                                                                gAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                                                                                                                  >  6:347769/1‑57 (MQ=35)
                                                                                gAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                                                                                                                  <  5:347769/57‑1 (MQ=35)
                                                                                   aGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGc                                                  >  2:52500/1‑134 (MQ=32)
                                                                                   aGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGc                                                  >  6:248673/1‑134 (MQ=32)
                                                                                   aGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt                                                 <  1:318863/135‑1 (MQ=32)
                                                                                                   ggAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTAGTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAg                                 <  1:421955/135‑1 (MQ=17)
                                                                                                     agagGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTAGATTCAGTGGCTAGCTTAACCGAATAGGGGAGGc                               >  4:133008/1‑135 (MQ=17)
                                                                                                          ggAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt                                                                                                 >  6:190490/1‑64 (MQ=35)
                                                                                                          ggAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt                                                                                                 <  5:190490/64‑1 (MQ=35)
                                                                                                            aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa                        <  5:375118/135‑1 (MQ=32)
                                                                                                            aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa                        <  3:145538/135‑1 (MQ=32)
                                                                                                            aGTGAAATAGAACCTGAAACCGGGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa                        <  5:296223/135‑1 (MQ=17)
                                                                                                             gTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          >  5:402972/1‑84 (MQ=33)
                                                                                                             gTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                          <  6:402972/84‑1 (MQ=33)
                                                                                                              tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa                      <  5:321030/135‑1 (MQ=32)
                                                                                                               gAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGa                                                                     <  2:151078/87‑1 (MQ=33)
                                                                                                               gAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGa                                                                     >  1:151078/1‑87 (MQ=33)
                                                                                                               gAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa                      <  8:402605/134‑1 (MQ=32)
                                                                                                                 aaTAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                           >  5:453891/1‑63 (MQ=35)
                                                                                                                 aaTAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                           <  6:453891/63‑1 (MQ=35)
                                                                                                                 aaTAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAg                     >  3:162369/1‑133 (MQ=32)
                                                                                                                 aaTAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGc                    <  4:162369/134‑1 (MQ=32)
                                                                                                                         cTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa            >  1:397843/1‑134 (MQ=32)
                                                                                                                         cTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa            >  6:195408/1‑134 (MQ=32)
                                                                                                                           gAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATa         >  7:210945/1‑135 (MQ=32)
                                                                                                                           gAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATa         >  7:156886/1‑135 (MQ=32)
                                                                                                                              aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                                >  2:329576/1‑45 (MQ=38)
                                                                                                                              aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                                <  1:329576/45‑1 (MQ=38)
                                                                                                                              aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAggg      >  8:142949/1‑135 (MQ=32)
                                                                                                                              aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAggg      >  4:298802/1‑135 (MQ=32)
                                                                                                                                  tgtgCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGtt  <  1:179600/135‑1 (MQ=31)
                                                                                                                                     |                                                                                                                                   
AAACTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTT  >  ACB122/169659‑169923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: