Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,312,455 A→C 24.3% S250A (TCG→GCG)  cynR_3 ← HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,312,4550AC24.3% 68.5 / 2.6 63S250A (TCG→GCG) cynR_3HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base A (17/27);  new base C (10/7);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAT  >  ACB122/3312322‑3312587
                                                                                                                                     |                                                                                                                                    
gCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCACCGAc                                                                                                                                     >  4:348287/1‑135 (MQ=255)
 catcatGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGAcc                                                                                                                                    <  3:179199/135‑1 (MQ=255)
     atGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCTTGCCCTCCAGCGACCAccg                                                                                                                                >  2:291554/1‑133 (MQ=255)
           gTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTg                                                                                                                          <  3:361448/135‑1 (MQ=255)
                 gcgcACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCCCCAg                                                                                                                    >  4:51682/1‑135 (MQ=255)
                     cccGCCTGCATCGCCGGGCTGAATGCGCGTGCCGGGTGCCCGGCCAACCCCACCCTCAGCGGCGTGACGGGGGCGCTGTTGGGCACCGGGTCGAGGGGCTGGCCCTCCAGCCACCACGGCCGGTGCACCAGGTcc                                                                                                                <  3:348287/134‑1 (MQ=255)
                       cGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGCTGGTCACCGTCTCGCTGCGCTTGCCCGCCCGCGCCCCCCGCCGGGGCCCCCGGTCCgc                                                                                                              >  2:209585/1‑134 (MQ=255)
                       cGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATTGTGCCCGGATCGCAGCGGTTGCCCACCAGCGACCAAGGCCGGTGCACCCGGGCCg                                                                                                               >  8:192239/1‑134 (MQ=255)
                          cTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCCGCGCCCACCGCCGGTGCCCCAGGTCCGACAg                                                                                                           >  6:315850/1‑135 (MQ=255)
                            gCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGGTGGTGCCCGTCTCGATGCGCTTGCCCGCCCGCGCCCACCGCCCGGGCCCCAGGTCCGGCAGGa                                                                                                         >  1:453705/1‑135 (MQ=255)
                                    gggCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTGACCGGCTCGCTGGGCTTGGCCGCCCGCGGCCACGGCCGGTGCACCCGGGCCGCCCGGCTCGCCCCt                                                                                                 >  1:358785/1‑135 (MQ=255)
                                    gggCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGc                                                                                                                         >  7:233363/1‑111 (MQ=255)
                                    gggCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGc                                                                                                                         <  8:233363/111‑1 (MQ=255)
                                     ggCTGATGGCCCGTTCCCGGTGCCCGGCCAACCCCAGGCCCATCGGCGTGCCGGGGTCGCTGAGGGCCACCGCCTCGAGGCGCTGGCCCGCCCCCGCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTc                                                                                                <  7:425850/135‑1 (MQ=255)
                                       cTGAATGCCCGTTCCCGGGGCCAGGCCACCCCCCCGCTCATCGGCTTCACGGGGGGGTTGGTGGTCCCCGGCGCGTGGCGCTGGCCCCCCCGCCCCCACGGCCGGGGCACCAGGTCCGACAGGATCGCCACTCCg                                                                                              <  2:67597/135‑1 (MQ=255)
                                            ggCCCGTTCCCGGGCCCAGGCCAACCCCCCGCTCATCGGCGTGACCGGGGCGCTGATGGTCACCGGCTCGATGCGCTTGCCCTCCAGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGcc                                                                                         <  2:16437/134‑1 (MQ=255)
                                             gCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCCGCGCCCACGGCCGGTGCCCCAGGTCCGACAGGCTCGCCCCTCCGCTGccc                                                                                        >  7:67386/1‑134 (MQ=255)
                                              cccgcccccGGGCCCAGGCCAACCCCACGCTCCCCGGCGGCACGGGGGCGCGGAGGGGCACCGGCTCGAGGCGCTTCCCCGCCAGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCAt                                                                                       <  4:12550/129‑1 (MQ=255)
                                              cccGTTCCCGGGGCCAGGCCAACCCCACCCTCTGCGGCGTGCCCGGGTCGCTGAGGGGCACCGTCGCGATGCGCTTGCCCTCCAGCGGCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCAt                                                                                       <  6:142099/135‑1 (MQ=255)
                                                 gtgcccGGTGCCAGGCCACCCCCCCGCTCAGCGGCGTGACGGGGTCGCTGAGGGTCACCGTCGCGATGCGCTTCCCCCCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTgg                                                                                    <  4:311545/132‑1 (MQ=255)
                                                   tCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACAGTCTCGATGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATAGCCCCTCAGCTGCCCATGGCg                                                                                  >  6:373287/1‑135 (MQ=255)
                                                    cccGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCGGATGGTCACCGTCTAGATGCGCTTGCCCTCCCGCGCCCCCGGCCGGTGCACCAGGTACGACACGATCGCCACTCCGCAGCCATTGGcca                                                                                 >  2:52988/1‑133 (MQ=255)
                                                      cGGTGCCCGGCCACCCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGGCCCCGTCTCGAGGCGCTTGCCCGCCAGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGAcc                                                                               <  2:257501/135‑1 (MQ=255)
                                                      cGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGGCGCTGATGGGCACCGTCTCGAGGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGAcc                                                                               <  7:2821/135‑1 (MQ=255)
                                                            cAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCTTGCCCTCCCGCGCCCACGGCCGGTGCACCAGGTCCGCCAGGATCGCCACACCGCTGCCATTGGCGACCAtgctg                                                                         >  7:331968/1‑135 (MQ=255)
                                                              ggCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCTTGCCCTCCAGCGACCACGGCCGGTGCCCCACGGCCGACAGGATCGCCCCTCCGCTGCCATTGGCGACCATGCTgcg                                                                       >  6:214740/1‑135 (MQ=255)
                                                              gcccACCCCCCCGCCCAGCGGGGGGCCCGGGGCGCGGATGGGCCCCGGCTCGATGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTgcg                                                                       <  5:130550/133‑1 (MQ=255)
                                                              gcccAACCCCACGCTCATCGGCGTGACCGGGTCGCTGAGGGTCACCGTCGCGAGGCGCTTGCCCTCCACCGACCACGGCCGGTGCACCAGGGCCGACAGGATCGCCACTCCGCTGACATTGGCGACCATGCTgcg                                                                       <  7:247941/133‑1 (MQ=255)
                                                                 cAACCCCCCGCGCATCGGCGTGCCCGGGGCGCTGAGGGGCACCGGCTCGAGGCGCTGGCCCCCCCGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCAc                                                                    <  3:25433/135‑1 (MQ=255)
                                                                      ccACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCCCTCCGCTGCCATTGGCGCCCAAGCTGCGCACGGCCt                                                               >  6:450156/1‑135 (MQ=255)
                                                                      ccACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGGCGCGCTGCGCATGCCCTCCCGCGACCACGCCCGGGGCCCCCGGGCCCCCAGGCTCGCCCCGCCGCTGCCATTGGCGCCCCTGCTGCGCCCGGccg                                                               >  5:149505/1‑134 (MQ=255)
                                                                      acACGCTCATCGGCGTGACCGGGTCTCTGATGGCCACCGTCTCGTTGCGGTTCCCCTCACCCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCt                                                               <  4:283349/134‑1 (MQ=255)
                                                                       cACGCTCATCGGCGTGACCGGGTCGCTGATTGTCACCCTGTAGAAGCGCTAGCCCACCACCGCCCACGGCCGGTGGCCCAGGGCCGCCAGGCACTCCACTCCCCAGCCAATGGCGACCCAGCAGCGCAAggacgc                                                              >  8:184086/1‑131 (MQ=255)
                                                                            tCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCAATTGCCACCATTCTGCGCACGGCCTCCCCCg                                                         >  2:441464/1‑135 (MQ=255)
                                                                            tCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCGCCAGCGCCCCCGGCCGGTGCCCCAGGGCCGCCACGATCGCCCCGCCGCTGCCCTTGGCGCCCCTGCTGCGCCCGGCCTCccccc                                                         >  5:117465/1‑134 (MQ=255)
                                                                                 ggCGTGACCGGGTCGCTGTTGGTGACCGGCTCGGTGCGCTTGCGCGCCACCGCCCCCCGCCCGTGCCCCACGGCCCCCACGATCGCCCCACCGCGGCCATTGGCGCCCCTGCAGCGCCCGGCCGCccccccgcag                                                    >  6:264724/1‑129 (MQ=255)
                                                                                 ggCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTg                                                    >  8:304099/1‑135 (MQ=255)
                                                                                   cttcaCCGGGTCGCTGATGGTCCCCTTCTCGATGCGCTGGCCCTCCAGCGACCAGGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  1:52988/131‑1 (MQ=255)
                                                                                   cgggcccGGGGCGCTGAGGGCCCCCGCCTCGATGCGCTGCCCCTCCCCCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  1:314394/130‑1 (MQ=255)
                                                                                   cgggACGGGGGCGCGGATGGGCACCGTCGCGATGCGCTTGCCCTCCAGCCACCACGGCCGGGGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  2:309722/132‑1 (MQ=255)
                                                                                   cgggACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  7:294803/132‑1 (MQ=255)
                                                                                   cGTGACGGGGGCGCTGTTGGCCACCGTCTCTATGCGCTGGCCCCCCCCCCCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  5:450156/135‑1 (MQ=255)
                                                                                   cGTGACCGGGTCGCTGATGGTGACCGGGTCGCTGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCACGTCCGCCACGATCGCCCCGCCGCGGCCATTGGGGGCCAAGCTGCGCCCGGCCGCCCCCGAGCGGGt                                                  >  6:38878/1‑135 (MQ=255)
                                                                                   cGTGACCGGGTCGCTGATGGTCACCGTCTCGAGGCGCTTGCCCCCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  3:157322/135‑1 (MQ=255)
                                                                                   cGTGACCGGGGCGCTGTGGGTCACCGTCACTTTGCGGTGGCCCGCCAGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  4:247116/135‑1 (MQ=255)
                                                                                   cGTGACCGGGGCGCTGAGGGTCCCCGGCTCGAGGCGCTTGCCCGCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  7:355616/135‑1 (MQ=255)
                                                                                   cGTCACGGGGTCGCTGATTGCCACCGGCTCCATGCGCTCGCCCACCCGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  3:155746/135‑1 (MQ=255)
                                                                                    gTGCCCGGGCCGATGCTGGACACCGTCTCCATGCGGTGGCCCGCCCGCCCCCACGGCGGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTg                                                 <  3:407539/135‑1 (MQ=255)
                                                                                        ccGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACCGGATCGCCACTCCGCTGCCCTTTGCGACCCAGCTGCGCACGGCCTCCACCGACCTGGTgcgcc                                             >  4:76738/1‑134 (MQ=255)
                                                                                           ggTCGCTGATGGTCACCGTCTCGATTGGCTTGCCCACCCGCCCCCACCGCCGGTGCCCCACGTCCCACAGGATCGGCACTCCCCTGCCCTTGGCGCCCATGCTGCGCACCGCCTCCCCCGCGCTGGTGCGGAcc                                           >  6:288585/1‑134 (MQ=255)
                                                                                                              ctcGAGGCGCTGGCCCGCCCGCGCCCACGGCCGGTGCACCAGGTCCGACAGGAGCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                       <  2:179087/135‑1 (MQ=255)
                                                                                                                gtcggcgcTCGCCCGCCCGCCCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCgg                     <  3:249734/131‑1 (MQ=255)
                                                                                                                ggATGGGCTGGCCGTCCCGCCACCACGGCCGGTGCTCCAGGTGCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCATCCACCGAGCTGGTGCTCACCCTTACGCTTGGCCGTTGCCCgg                     <  2:258674/134‑1 (MQ=255)
                                                                                                                cgctGCGCTTGCCCCCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCgg                     <  5:214740/132‑1 (MQ=255)
                                                                                                                cGATCCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCgg                     <  6:124845/135‑1 (MQ=255)
                                                                                                                  cggcgcTTCCCGCCCCGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCgg                     <  5:375474/131‑1 (MQ=255)
                                                                                                                  aTGCGCTGGCCCCCCCGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCgg                     <  1:291554/133‑1 (MQ=255)
                                                                                                                   tGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCt                  <  1:244643/135‑1 (MQ=255)
                                                                                                                          gCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCa           >  4:364005/1‑135 (MQ=255)
                                                                                                                          gCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCa           >  3:378216/1‑135 (MQ=255)
                                                                                                                          gCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCAAGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCa           >  7:204189/1‑135 (MQ=255)
                                                                                                                          ccccTCCAGCGCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCa           <  6:465399/134‑1 (MQ=255)
                                                                                                                                   cGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAt  >  4:115592/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAT  >  ACB122/3312322‑3312587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: