Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB122 | 3,773,715 | C→A | 5.8% | T370K (ACA→AAA) | nikA → | Nickel‑binding periplasmic protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 3,773,715 | 0 | C | A | 5.8% | 202.2 / 4.0 | 70 | T370K (ACA→AAA) | nikA | Nickel‑binding periplasmic protein |
Reads supporting (aligned to +/- strand): ref base C (39/27); new base A (2/2); total (41/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01 |
TCAAGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTCGGCATGA > ACB122/3773583‑3773846 | tCAAGCGCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGTCGCGTCTGTCCGCAGTAAATGCGGCGAACCGTTGTCCATCGAGCTGGTATTCCTCGGCACaa < 8:317350/135‑1 (MQ=255) tCAAGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACaa < 7:350924/135‑1 (MQ=255) tCAAGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACaa < 3:2918/135‑1 (MQ=255) tCAAGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGGCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCCCaa > 8:416682/1‑135 (MQ=255) aaGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGGCTGTGCGCAGGAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTTTTCCTCGGCCCaact > 4:1540/1‑133 (MQ=255) aGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTg < 5:418577/135‑1 (MQ=255) gCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACaa < 3:354549/131‑1 (MQ=255) gCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACaa > 4:354549/1‑131 (MQ=255) ccTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTGTGTTCGGAGTAAAAGCGGCGCACCCGTGGCCCTCGCGCTGGTATTCCTCGGCACAAGTGCCTTGCa > 5:23899/1‑134 (MQ=255) ccTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGGAGTAAAGGCGGCGAACCCTGGGCCCTCGAGCTGGTATTACTCGGCCCAGGTGCCTTGCa > 1:4283/1‑134 (MQ=255) aCCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGACCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGcc > 4:334147/1‑135 (MQ=255) aCCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACCAGTGCCTTGCAGCAGAGcc > 8:102029/1‑135 (MQ=255) aCCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCAGCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGcc > 8:368198/1‑135 (MQ=255) cGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTgg < 7:60156/135‑1 (MQ=255) cGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTgg < 8:235706/135‑1 (MQ=255) gcgcgcgcCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCCGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGc > 1:311415/1‑134 (MQ=255) gcgcgcgcCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGcc < 4:181386/135‑1 (MQ=255) gcgcgcgcCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGGCTGTGCGCAGTAAAGGCGGCGCACCCTTGGCCCTCGCGCGGGGATTCCTCGGCCCAAGTGCCGTGCAGCAGCGCCTGGcc > 2:396801/1‑135 (MQ=255) gcgcgcgcCGCCCATGCCCTTGATGCTGCCGGGTGGACGCGGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGGGCCTTGCAGAAGAGCCTGGcc < 7:90791/135‑1 (MQ=255) cgcgcgcCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGcc > 1:269220/1‑134 (MQ=255) ccATGCCCTTGATGCTGCCGGGGGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTTAAGGCGGCGAACACTTGGCCCTCGAGCTGGTATTCCTCGGGACACCTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCa < 1:295286/135‑1 (MQ=255) gctgcCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGACCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTTGCCGAAGTGCTGCAGGGCGCGCTGGc > 1:323787/1‑134 (MQ=255) gctgcCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGc > 8:74092/1‑134 (MQ=255) cGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAAt > 1:310864/1‑135 (MQ=255) cGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAAt > 6:157243/1‑135 (MQ=255) cGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAAt > 3:231470/1‑135 (MQ=255) gggTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGACCCCTTGGCCATCGAGCTGGGATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAATATc > 1:420607/1‑135 (MQ=255) gggTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGACCCCATGGCCATCGAGCTGGTATTACTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGATCTGGCGAATATc > 1:229512/1‑135 (MQ=255) gggTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGCGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATc > 5:412484/1‑135 (MQ=255) gggTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGCGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATc > 5:133564/1‑135 (MQ=255) gggTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCGGGTATTCCTCGGCACAAGTGCCTTGCAGAAGCGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATc > 2:326738/1‑135 (MQ=255) gTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCgg < 1:138799/135‑1 (MQ=255) gTGGACGCGGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCgg < 8:249438/135‑1 (MQ=255) cTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCcac > 4:316768/1‑135 (MQ=255) ggCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACAt > 4:212156/1‑135 (MQ=255) gCCCCAGGCGCGTCGGTGCGCAGTAAAGGCGGCGAACCCTTGGCCAGCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATc < 7:243427/135‑1 (MQ=255) aGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCt < 3:417719/135‑1 (MQ=255) gcgcGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCtgc < 2:310225/135‑1 (MQ=255) gcgcGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCtgc < 6:243435/135‑1 (MQ=255) gcgTCTGTGCGCAGTAAAGGCGGCGACCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCtgagt > 6:237451/1‑135 (MQ=255) gcgTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCtgcgt > 7:32354/1‑135 (MQ=255) cTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCgg < 2:229512/135‑1 (MQ=255) cTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTACTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGCCCTGCGTGCgg > 6:93155/1‑135 (MQ=255) gtgCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGt > 1:78553/1‑134 (MQ=255) gcgcAGTATAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTga < 2:5547/135‑1 (MQ=255) gcAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTgaag < 6:23899/135‑1 (MQ=255) gcAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTgaag < 2:78553/135‑1 (MQ=255) gcAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTAGGCACAAGTGCCTTGCAGAAGAGACTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTgaa > 8:384749/1‑134 (MQ=255) aaaGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAggg > 2:316805/1‑135 (MQ=255) aGGCGGCGAACCCTTGGCCATCGAGCTTGTATTCCTCGGCACAGGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAgggg < 2:4283/134‑1 (MQ=255) cggcgAACCCTTGGCCATCGAGCTGGTATTCCTCGGCAAAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTga < 7:75646/123‑1 (MQ=255) cggcgAACCCTTGGCCATCGAGCTGGTATTCCTCGGCAAAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTga > 8:68311/1‑123 (MQ=255) cggcgAACCCTTGGCCATCGAGCTGGTATTCCTCGGCAAAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTga < 7:68311/123‑1 (MQ=255) cggcgAACCCTTGGCCATCGAGCTGGTATTCCTCGGCAAAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTga > 8:75646/1‑123 (MQ=255) ggcgCACCCTTGGCCATCGAGCGGGTATGCCTCGGCACAAGTGCCTGGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTTCGGTTGAAGAAGGGGCTTTg < 7:87403/135‑1 (MQ=255) gcgAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTgg > 4:435664/1‑135 (MQ=255) aaCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCgg < 1:87828/91‑1 (MQ=255) aaCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCgg > 2:87828/1‑91 (MQ=255) aaCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTgc > 2:369522/1‑135 (MQ=255) aaCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTgc > 2:378640/1‑135 (MQ=255) aaCCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTgc > 5:152444/1‑135 (MQ=255) cATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGCGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAAcg > 8:384735/1‑135 (MQ=255) aGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACg > 2:399241/1‑135 (MQ=255) gCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACgg > 6:417691/1‑135 (MQ=255) tATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGa < 7:127700/133‑1 (MQ=255) ttCCTCGGCACACGGGCCTGGCAGAAGAGCCTGGCCGAAGGGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGCCCGGGGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTc < 8:263010/135‑1 (MQ=255) tCCTGGGCACTTGTGCCTTGCTGACGTGCCTGGCAGAAGTGCTGCAGGGGGAGCTGGCGAAAATGGGAATCCTCCTCGTCCTGCGTGCGGTTGAAGATGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTCg < 5:212446/135‑1 (MQ=255) cGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTCGGCa > 2:335371/1‑134 (MQ=255) ggCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTCGGCATg > 5:248716/1‑135 (MQ=255) gCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTCGGCATGa < 3:124964/135‑1 (MQ=255) cacaAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCg < 3:439958/122‑1 (MQ=255) cacaAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCg > 4:439958/1‑122 (MQ=255) | TCAAGCCCTACCCTTACGACCCGGCGCGCGCCGCCCATGCCCTTGATGCTGCCGGGTGGACGCTGGCCCCAGGCGCGTCTGTGCGCAGTAAAGGCGGCGAACCCTTGGCCATCGAGCTGGTATTCCTCGGCACAAGTGCCTTGCAGAAGAGCCTGGCCGAAGTGCTGCAGGGCGAGCTGGCGAAAATCGGCATCCACATCGACCTGCGTGCGGTTGAAGAAGGGGCTTTGGTGCGGCGCCAACGCGACGGCGACTTCGGCATGA > ACB122/3773583‑3773846 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |